Sunulan çalışmada in vitro embriyo üretiminde kültür medyumlarına eklenen antioksidanların blastosist gelişim oranlarına etkisinin belirlenmesi amaçlandı. Çalışmanın materyalini mezbahadan alınan ovaryumlardan toplanan oositler oluşturdu. Kumulus oosit kompleksleri (COC) stereo mikroskop altında toplandı ve sınıflandırıldı. Çalışmaya alınacak oositler maturasyon ve fertilizasyon aşamalarına tabi tutuldu. Muhtemel zigotlar antioksidanların (L-ergotiyonin 100 μM (n:163), Vitamin E 100 μM (n:151) Sisteamin 50 μM (n:154) ilave edildiği kültür (IVC) droplarına aktarılarak tri gaz (Hera Cell- %6 O2, %6CO2, %88N) inkübatörde kültüre bırakıldı. Kültür ortamındaki 6. ve 7. gün blastosist oranları ve embriyo kaliteleri değerlee yerleştirildi. IVMFC aşamalarında oluşan farklılıklar Ki-kare testi ile değerlendirildi. Mezbahadan toplanan 162 ovaryumdan 966 adet oosit toplandı. Ovaryum başına oosit sayısının 5,96 olduğu A ve B kalite oosit sayısının ise 4,26 olduğu belirlendi. İn vitro maturasyona alınan toplam 690 oositin 655’inin (%94,93) mature olduğu belirlendi. Grupların cleavage oranları sırasıyla %83,44; %80,79; %79,87 ve %83,96 olduğu belirlendi. Kültür aşamasına alınan 655 oositten 140 (%21,37) adet blastosist elde edildi ve gruplardaki blastosist oranları sırasıyla %33,13; %8,61; %7,79 ve %32,62 olduğu görüldü. Yapılan çalışma sonucunda L-ergotiyonin eklenen gruptaki bilastosist oranlarının kontrol grubu ile benzer olduğu ancak sisteamin ve Vitamin E eklenen gruplarda blastosisit oranlarının önemli oranda düştüğü belirlendi. Bu düşüşün antioksidanların dozu yada oosit gelişim yeterliliğinden etkilenmiş olabileceği düşünüldü.

This experiment was carried out to investigate the effects of variant doses of rosemary (Rosmarinus officinalis L.) essential oils (REO), vitamin E (α‐tocopheryl acetate) and antibiotic (chlortetracyclin) on the concentration of vitamin E, fatty acid composition, cholesterol levels in the yolk. In this experiment, 32 weeks old Bovans genotype and 240 white laying hens were randomly separated to six treatment groups with five replicas. While the control group was fed with basal diet the treatment groups were supplemented with 500 mg/kg antibiotics; 200 mg/kg vitamin E; 100, 200, and 300 mg/kg REO to basal diet for 90 consecutive days. The results showed that addition of antibiotics, vitamin E and REO to the diet had no effects on egg cholesterol, while fatty acid composition and vitamin E contents of the yolk were significantly affected. Addition of 100 mg/kg REO to the diet increased total mono unsaturated fatty acids (MUFA) by increasing oleic acid level but decreased Polyunsaturated fatty acids (PUFA) by decreasing linoleic acid content of the yolk. Addition of 200 mg/kg vitamin E to the diet significantly increased vitamin E content of egg. The study showed that the changes in yolk fatty acid composition and vitamin E content due to the feed additives, could affect positively in chemical composition of eggs. In conclusion, 100 mg/kg REO and 200 mg/kg vitamin E supplementation to the diet might cause high antioxidant capacity and could help to produce eggs that were more resistant to lipid oxidation.

The present study, it was aimed to determine the effect of antioxidants added to culture media on blastocyst development rates in in vitro embryo production. The material of the study consisted of oocytes collected from the ovaries taken from the slaughterhouse. Cumulus oocyte complexes (COC) were collected and classified under a stereomicroscope. Oocytes included in the study were subjected to maturation and fertilization stages. Probable zygotes were transferred to the culture (IVC) containing antioxidants (L-ergothionine 100 μM (n: 163), Vitamin E 100 μM (n: 151) Cysteamine 50 μM (n: 154) and were cultured in a tri gas incubator (Hera Cell- 6% O2%, 6%CO2, 88%N). Blastocyst rates and embryo quality were determined on the 6th and 7th days in culture medium. Differences in IVMFC stages were evaluated by chi-square test. 966 oocytes were collected from 162 ovaries collected from the slaughterhouse. It was determined that the number of oocytes per ovary was 5,96, and the number of A and B quality oocytes was 4.26. It was determined that 655 (94.93%) of a total of 690 oocytes undergoing in vitro maturation were mature. The cleavage rates of the groups were 83.44%; 80.79%; 79.87%, and 83.96%, respectively. 140 (21.37%) blastocysts were obtained from 655 oocytes taken into the culture stage and the blastocyst rates in the groups were 33.13%; 8.61%; 7.79%, and 32.62%, respectively. As a result of the study, it was determined that the rates of blastocysts in the L-ergothioneine added the group was similar to the control group, but the blastocyst rates decreased significantly in the cysteamine and Vitamin E added groups. It was thought that this decrease might have been affected by the dose of antioxidants or the adequacy of oocyte development

The purpose of this study was to determine the effects of selenium, vitamin E, vitamin A, and vitamin D3 treatments on reproductive parameters in Awassi sheep fed solely dry grass and grain stubble during the breeding season with estrus synchronization. Seventy-five sheep were implanted with intravaginal sponges containing 20 mg of flugestone acetate for 9 days for estrus synchronization. On the day the sponges were inserted, the first group received an intramuscular injection of a supplement containing 200,000 IU of vitamin A, 30,000 IU of vitamin D3, and 20 mg of vitamin E, as well as a supplement containing 1 mg of sodium selenite and 60 mg of vitamin E. The second group was the control group, with no supplementary vitamin injected. On the day of sponge removal, the sheep received intramuscular injections of 500 IU PMSG and 250 mcg cloprostenol sodium in both groups. The sheep in the first group were given a second injection of the supplement on the same day that contained 20 mg of vitamin E, 30,000 IU of vitamin D3, and 200,000 IU of vitamin A. The sheep that showed signs of estrus were mated naturally after the estrus synchronization. Estrus rates were 86.11% and 85.29%, conception rates were 74.19% and 58.62%, pregnancy rates were 63.88% and 50%, lambing rates were 100% and 100%, and litter size was found to be 126.08% and 123.52% in Group I and II, respectively (P=0.858). Although there was no significant difference in the reproductive characteristics between the groups (P>0.05), Group I had a greater pregnancy rate and litter size. During the breeding season, it is believed that the regular application of vitamin and mineral supplements on a program basis in sheep fed only dry pasture and grain stubble contributed to fertility.