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Investigation of Antiproliferative Effects of Home-Made and Commercial Apple Vinegars on Myeloma Cells
2021
Muhammet Mükerrem Kaya | Soner Tutun | Melike Sultan Usluer | Hidayet Tutun
Vinegar is an aqueous food product made by a succession of yeast and acetic acid bacteria activities from fruits that contain high carbohydrates such as apples and grapes. Vinegar has been used as a dietary spice and natural remedy since ancient times due to its therapeutic properties including antimicrobial, antidiabetic, and anticancer activities. It has been shown that some bioactive compounds exhibiting antioxidant activity in vinegars lead to anticancer activity. The aim of the present study was to investigate antiproliferative effect of commercial and home-made apple vinegars in native and neutralized form on myeloma cells. In order to neutralize the vinegars, sodium hydroxide (NaOH) was used. A serial two-fold dilutions of the vinegars (50%, 25%, 12.5%, 6.25%, 3.12%, 1.56%, 0.78%, 0.39%) prepared with cell medium were treated to the cells. The MTT (3-(4.5-Dimethylthiazol-2-yl)-2.5-Diphenyltetrazolium Bromide) assay was used to determine the cellular viability in the cells treated with the vinegars. In this study, while commercial vinegar possessed a stronger antiproliferative activity than home-made vinegar, all native vinegars possessed stronger antiproliferative effect than neutralized vinegars. Interestingly, when home-made vinegar (both native and neutralized) concentrations were from 6.25 to 1.56%, the cell viability increased. Apple vinegar exhibited antiproliferative activity on myeloma cells; however, further studies are required to clarify the mechanisms underlying this activity.
Показать больше [+] Меньше [-]Evaluation of Different Land Preparation Techniques for Preparing Medium Textured Soil in Rice Production under Agro-Ecological Conditions of Sheikhupura-Pakistan
2021
Usman Hassan | Muhammad Shahbaz | Muhammad Saleem Kashif | Liaqat Ali | Muhammd Tariq Chaudhary | Wardah Qamar
Tillage is an important factor affecting different soil properties and crop yields. Tillage relates to applying forces to soil using different implements for preparation of a proper seed bed. For rice crop, land preparation is a rigorous and time consuming operation that needs special attention for preparing a puddled soil condition to transplant the weak and tender rice seedlings. A field experiment was established under agro-ecological conditions of Sheikhupura to evaluate different land preparation techniques during fall in 2017 and 2018 years. The experiment was performed at Adaptive Research Farm Sheikhupura. It was laid out in randomized complete block design (RCBD) with four different land preparation techniques including T1= Cultivator (4 times) + Planking (2 times) (Farmer’s Practice), T2=MB plough (1 time) + Disc Harrow (1 time) + Planking (2 Times), T3=MB plough (1 time) + Cultivator (2 times) + Planking (2 Times) and T4= Rotavator (1 time) + Disc Harrow (1 time) + Planking (2 Times), each treatment replicated thrice during both the years. All the other agronomic and crop husbandry operations were kept uniform throughout the growing season every year. The results revealed that preparing land with MB plough (1 time), Disc Harrow (1 time) and Planking (2 times) is the best land preparation method can increase the paddy yield 13.5-17.5% as compared to the traditional method practised by the farmers. Moreover, highest plant height (134.00 cm and 132.00 cm), number of tillers (224 and 220 m-2), number of grains per spike (130 and 116) and 1000-grain weight (23.0 and 22.0 g) respectively during Kharif-18 and Kharif-19 was also recorded in the same treatment. So, preparation of land for paddy in agro-ecological conditions of Sheikhupura using MB plough (1 time), Disc Harrow (1 time) and Planking (2 times) can significantly enhance the paddy yield.
Показать больше [+] Меньше [-]Seasonal Abundance and Spatial Pattern of Distribution of Liriomyza trifolii (Diptera: Agromyzidae) and Its Parasitoid on Bean and Squash in South Florida
2021
Shashan Devkota | Dakshina Seal
American serpentine leafminer, Liriomyza trifolii, is a polyphagous insect pest that feeds on a wide range of vegetable and ornamental plants around the world. To develop an effective IPM program, information on the seasonal field distribution and population dynamics of leafminer and its parasitoids is very important. Therefore, seasonal abundances and spatial distributions of, L. trifolii on snap bean and squash were studied during four crop growing periods between 2013 to 2015 in Homestead, Florida. The mean numbers of mines, larvae, pupae, emerged adults, and parasitoids on snap bean were highest at 2 weeks after planting during all four growing periods. Whereas, the mean numbers of mines, larvae, pupae, emerged adults, and parasitoids on squash were highest at 3 weeks after planting during all four growing periods. L. trifolii distributions tended to be aggregated on snap bean at 2 weeks after planting during most of growing periods but had uniform distributions on squash at 2 weeks after planting during most of growing periods. Similar results were seen on the distribution of leafminer parasitoids on both bean and squash.
Показать больше [+] Меньше [-]Hayvan Yemi Olarak Kullanılan Şekerpancarı Yapraklarının Nitrat İçeriğine Azot-Bor Uygulamasının ve Zamanın Etkisi
2021
Bedriye Bilir | Kadir Saltalı
Şekerpancarı üretiminde verim ve kalite için azot (N) ve bor (B) gübrelemesi önemlidir. Fakat fazla azotlu (N) gübreler kalite parametreleri üzerinde olumsuz etkilere neden olmaktadır. Hayvan yemi olarak kullanılan şeker pancarı yapraklarında nitrat birikiminin artması da olumsuz etkilenen kalite parametrelerinden birisidir. Bu çalışmanın amacı, şeker pancarına farklı dozda azot ve bor uygulamasının bitki yapraklarında nitrat birikimi üzerine etkisini incelemektir. Çalışma, Kahramanmaraş ili Elbistan İlçesinde 2017 yılında B içeriği 0,56 mg kg-1 olan bir arazide yürütülmüştür. Deneme tesadüf blokları faktöriyel deneme desenine göre 3 tekerrürlü olarak kurulmuştur. Denemede 5 farklı N dozu (0, 9,18, 27, 36 kg N da-1) ve 4 farklı B dozu (0, 200, 400, 600 g B da-1) uygulanmıştır. Araştırmada temmuz, ağustos, eylül ve ekim aylarında yaprak örnekleri alınmıştır. Araştırma sonuçlarına göre N dozu arttıkça yaprakların nitrat içeriği istatistiksel olarak önemli derecede artmıştır. Bor uygulamasının yaprakların nitrat içeriği üzerine etkisi önemli bulunmamıştır. Hasat dönemine doğru yaprakların nitrat içeriği istatistiksel olarak önemli düzeyde azalmıştır. Sonuç olarak hasat sonrası şekerpancarı yapraklarının hayvan yemi olarak kullanması, nitrat bakımından hayvanlar için bir risk oluşturmamaktadır.
Показать больше [+] Меньше [-]Aydın İli Zeytin Alanlarında Zeytin Sineği (Bactrocera oleae (Gmelin)) (Diptera: Tephritidae)’ nin Populasyon Değişimleri ve Zararı
2021
Fulya Kaya Apak | Hüseyin Başpınar
Bu çalışma ile Aydın ili Umurlu, Dalama ve Çakmar mahallelerinden seçilen üç zeytin bahçesinde Zeytin sineği (Bactrocera oleae (Gmelin)) (Diptera: Tephritidae)’ nin mevsim içinde ortaya çıkış zamanı, populasyon değişimi ve meyvelerdeki bulaşıklılık oranı belirlenmeye çalışılmıştır. Çalışmada her bir bahçeye birer adet %2 diamonyum fosfat içeren McPhail (McPhail) ve feromon kapsüllü görsel sarı yapışkan tuzak (feromon), ayrıca üçer adet içerisine amonyum asetat konulmuş epondorf tüplerin monte edildiği görsel sarı yapışkan tuzaklar (AA) asılmıştır. Çalışma 2009-2011 yılları arasında yürütülmüş ve tuzaklar haftalık olarak kontrol edilmiştir. Yapılan populasyon takibi çalışmaları sonucunda tuzaklarda ilk ergin sinekler genel olarak Ekim ayının ortalarında yakalanmış ve mevsim süresince devam etmiş ve Aralık ayının ortalarına doğru popülasyonun sıfır düzeyine indiği gözlemlenmiştir. Örnekleme alanlarında Zeytin sineği populasyonu çok düşük düzeylerde seyretmiştir. Ancak popülasyonun maksimuma ulaştığı 2009 yılında Umurlu’ daki bahçede daha yüksek bir popülasyon ortaya çıkmış ve 30 Ekim 2009 tarihinde feromon tuzakta 307,0 birey/tuzak birey saptanmıştır. Popülasyon düzeyleri aynı tarihte aynı bahçede McPhail tuzakta 70,0 birey/tuzak, AA tuzaklarda ise ortalama 51,3 birey/tuzak olarak belirlenmiştir. Meyvelerde en fazla bulaşıklılık oranı ise yine aynı yıl %17,2 olarak Umurlu’da bulunmuştur. Aynı tarihte Dalama ve Çakmar’daki bahçelerde de sırasıyla feromon tuzaklarda 45,0 birey/tuzak ve 3,0 birey/tuzak; McPhail tuzaklarda 8,0 birey/tuzak ve 1,0 birey/tuzak; AA tuzaklarda ise ortalama 4,7 birey/tuzak ve 0,3 birey/tuzak olarak belirlenmiştir. Dalama ve Çakmar’da en fazla vuruk oranları sırasıyla %8,9 ve %3,7 olmuştur. Çalışmadaki diğer yıllarda hem popülasyon hem de meyvelerdeki vuruk oranları çok düşük gerçekleşmiştir.
Показать больше [+] Меньше [-]Antagonistic Activity of Bacillus spp. Against Fire Blight Disease In vitro and In planta
2021
Haris Butt | Kubilay Kurtulus Bastas
Fire blight, affecting more than one hundred and thirty species in the Rosaceae, is probably the most destructive disease affecting pear and apple cultivars in many countries. Currently, there are no effective synthetic compounds with systemic properties. Other major problem is the occurrence and spread of strains of Erwinia amylovora with resistance to streptomycin and copper. Taken into consideration the human and environmental health, the use of biocontrol agents either as an alternative or as a supplement within an integrated fire blight management strategy has attracted worldwide attention. In this study, E. amylovora solution of 107 CFU ml-1 was treated with bio-control agents, Bacillus subtilis str. QST 713, B. amyloliquefaciens str. MBI 600 and their mixture (at solution densities of 106, 107 and 108 CFU ml-1 for each one) on Petri dishes, containing King’s B medium and, compared with positive (streptomycin sulphate) and negative (sterile distilled water) controls. In vivo studies were performed on two-year-old apple cv. Gala seedlings grown in 45-cm-diameter pots containing a sterilized mix of soil–sand–peat under controlled greenhouse conditions (85% relative humidity, 25°C temperature and 16h of day light). The plants were irrigated as needed by drip-irrigation and each pot received a mineral solution (NPK: 20–20–20) at 2 g l-1 twice. When plant shoots reached a length of 30-35 cm, bio-control agents, individually and their mixture, were applied to the plants by a hand-sprayer. Obtaining the data, 108 CFU ml-1 of Bacillus spp. suspension mixture showed strongest in vitro antibacterial effect (26mm) among the tested treatments after positive control streptomycin (28.6mm). Parallel to in vitro findings, the mixture was most effective against the pathogen on cv. Gala (66.03%). Findings show that the use of mixture of beneficial microorganisms with individual antagonistic properties against the pathogen can be an effective strategy as a natural alternative to agrochemicals in the scope of good agriculture practices.
Показать больше [+] Меньше [-]Investigation of The Sufficiency of Antalya Parks
2021
Orhun Soydan | Nefise Çetin
Urban green spaces are areas established to meet the recreational needs of urban people. Although green spaces vary from country to country and region in terms of plan and design features, they were basically created to allow people to meet with nature. Parks are the basic components of urban landscapes that provide environmental and social functional value. Urban parks, in particular, provide spaces for outdoor physical activities. In order to take advantage of the opportunities of activities in the parks, users must have convenient access to these resources. One of the most important aspects for researching the use and potential benefits of urban green spaces is the assessment of their geographic accessibility. The widespread use of smart city systems and the gradual expansion of their usage areas increase the importance of spatial analysis. Spatial analyses are used in today’s urban management in the processes of determining social needs, identifying current problems, and putting forward solutions. When spatial analyses are used together with GIS, the field of application develops even more, and it supports local governments in responding to the changing demands of the society for a better life. In the study, the adequacy and accessibility of 160 city parks in Konyaaltı District of Antalya Province were examined. In terms of the adequacy of the parks, the area value of 10 m2 per person determined with the Construction Plan numbered 3194 was taken as basis. In terms of accessibility, distance values of 200, 400, 800, 1,200 meters were examined. Neighborhood boundaries and population information were obtained from the relevant units, and Arc-GIS software was used in the analysis. It was determined that the parks in Konyaaltı district were insufficient in terms of adequacy and accessibility. Finally, suggestions were made in terms of increasing the adequacy of the parks and ensuring accessibility.
Показать больше [+] Меньше [-]Determination of Prevalence and Reservoir Weed Species of Tomato spotted wilt tospovirus-TSWV on Peppers Grown in Greenhouses in Kumluca District of Antalya, Turkey
2021
Serkan Yeşil | Özder Gömlekli
There are many viruses that infect pepper and limit its production. Among these viruses, Tomato spotted wilt tospovirus (TSWV) infects crops in 35 plant families that are economically important, including pepper. In the present study; totaly 156 leaf samples were collected, including 57 from pepper plants showing virus-like symptoms and 99 from weeds and/or plants other than peppers in and around the greenhouse, through surveys carried out in pepper greenhouses in Kumluca district of Antalya province, from September to December 2020. Then, the plant leaf samples were tested to determine TSWV infections by the Double Antibody Sandwich Enzyme-linked Immunosorbent Assay (DAS-ELISA) method. According to result of the tests, it was determined that 55.76% of the tested leaf samples were infected with TSWV, while this rate was determined as 96.49% for pepper samples and 32.32% for other plant samples. During the survey studies, it was revealed that the leaf samples of 13 out of 31 weed and different plant species except pepper were infected with at least one of the viruses. In addition, pepper plants showing symptoms TSWV-like symptoms in pepper greenhouses were counted during the survey, and the prevalence of this virus disease was calculated on the basis of Kumluca district and neighborhoods. As a result of these calculations, the prevalences of TSWV; for Kumluca, Mavikent, Beykonak, Salur, Hacıveliler, Adrasan, Merkez, and Kavakköy were determined as 26.93%, 26.92%, 32.27%, 20.66%, 21.13%, 17.66%, 13%, and 25%, respectively.
Показать больше [+] Меньше [-]An Analysis of the Change in the Attitudes and Behaviors of Users before and after the Pandemic: Case Study; Ankara Çubuk-1 Dam
2021
Sertaç Güngör | Elif Nur Doğan
During the pandemic process that has been going on for more than a year since the pandemic period was declared in 2020, the troubles and restrictions faced by people negatively affect the morale motivation of people. In this study, the definition of recreation areas, which are the leading places where people feel happy and peaceful, has been defined, what they contain is mentioned, and the adequacy of the recreational areas has been discussed in the survey and it has been determined that they are one of the most important needs in the cities. In the example of the recreation area of Çubuk-1 dam, it was investigated whether there is a change in the attitudes and behaviors of people before and after the pandemic, not face to face due to the pandemic, but through a voluntary survey based on social media. Although individuals thought that it was crowded after the pandemic, they preferred to use the recreation area of Çubuk-1 dam by paying attention to mask, distance and hygiene rules. The positive effects of physical activity, socialization, and outdoor travel on health are generally accepted by individuals. During the pandemic process, it has been observed that people have changed the way they spend their free time and the places they want to be, as they are overwhelmed by staying in their homes due to the prohibitions. The psychological and physical positive effects of recreation areas on human health and the changes caused by the pandemic in people's attitudes and behaviors paralleled the responses of the participants to the questionnaire.
Показать больше [+] Меньше [-]The Essential Oil Components of Helichrysum pallasii Flowers
2021
Emine Yurteri | Fatih Seyis | Haydar Kuplemez
The genus Helichrysum contains many species displaying therapeutical effects and use in folk medicine. Helichrysum pallasii (Spreng.) Ledeb. is one of these species, used in the region for traditional medicine and ornamental purposes. Plants have been used in the treatment in folk medicines since the earliest times of human history. Chemical composition, antimicrobial and other medicinal properties of plants are being investigated in laboratories throughout the world using the extracts and essential oils derived from medicinal plants. Essential oils are usually complex mixtures of natural compounds of both polar and apolar structure. Essential oils consist mainly of terpenoids and their oxygenated derivatives. Essential oils, which have antioxidant and antimicrobial activities, have therapeutic properties and are the raw materials of cosmetics, as well as acting as natural additives in foods and food products. In this study, the volatile components of the H. pallasii plants were investigated which collected from its natural habitat in the Armutlu district of Bayburt province (Turkey) at the time of flowering. The proportion of essential oils of the plant harvested during the full flowering period and the aromatic components in the essential oil of the plant were determined using the SPME (Solid Phase Microextraction) method in a Gas Chromatography (GC-MS) device. Almost 37 different components were found as a result of the analysis. The main components were found as α-Springene (34.89%), (-)-Isoledene (7.11%), δ-Selinene (5.79%) and Aristolene (5.09).
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