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Antagonistic Activity of Bacillus spp. Against Fire Blight Disease In vitro and In planta Полный текст
2021
Haris Butt | Kubilay Kurtulus Bastas
Fire blight, affecting more than one hundred and thirty species in the Rosaceae, is probably the most destructive disease affecting pear and apple cultivars in many countries. Currently, there are no effective synthetic compounds with systemic properties. Other major problem is the occurrence and spread of strains of Erwinia amylovora with resistance to streptomycin and copper. Taken into consideration the human and environmental health, the use of biocontrol agents either as an alternative or as a supplement within an integrated fire blight management strategy has attracted worldwide attention. In this study, E. amylovora solution of 107 CFU ml-1 was treated with bio-control agents, Bacillus subtilis str. QST 713, B. amyloliquefaciens str. MBI 600 and their mixture (at solution densities of 106, 107 and 108 CFU ml-1 for each one) on Petri dishes, containing King’s B medium and, compared with positive (streptomycin sulphate) and negative (sterile distilled water) controls. In vivo studies were performed on two-year-old apple cv. Gala seedlings grown in 45-cm-diameter pots containing a sterilized mix of soil–sand–peat under controlled greenhouse conditions (85% relative humidity, 25°C temperature and 16h of day light). The plants were irrigated as needed by drip-irrigation and each pot received a mineral solution (NPK: 20–20–20) at 2 g l-1 twice. When plant shoots reached a length of 30-35 cm, bio-control agents, individually and their mixture, were applied to the plants by a hand-sprayer. Obtaining the data, 108 CFU ml-1 of Bacillus spp. suspension mixture showed strongest in vitro antibacterial effect (26mm) among the tested treatments after positive control streptomycin (28.6mm). Parallel to in vitro findings, the mixture was most effective against the pathogen on cv. Gala (66.03%). Findings show that the use of mixture of beneficial microorganisms with individual antagonistic properties against the pathogen can be an effective strategy as a natural alternative to agrochemicals in the scope of good agriculture practices.
Показать больше [+] Меньше [-]Current Situation and Position of Fisheries Production of Sinop Province in Turkey’s Fisheries Production Полный текст
2021
Şennan Yücel | Birol Baki | Tuçe Altın | Gülşen Uzun Gören
In the present study, the production amount in the last five years (2015-2019) of Sinop province, which is located in the Black Sea Region and a significant part of fisheries production is carried out, was evaluated. For this purpose; Sinop Provincial Directorate of Agriculture and Forestry and TUIK (Turkish Statistical Institute) data were used. There are 2.326 commercial fishermen and 444 fishing boats in Sinop province. In the sea area of Sinop, 5 enterprises still produce culture fish in cages. The 2 potential marine culture areas identified in Sinop have an annual production capacity of 32.430 tons (31 enterprises). In addition, 5 bivalves (4.940 tons/year) and 1.000.000 number/year capacity juvenile fish production facilities are at the project stage. The total fisheries production of Sinop Province in 2019 was 34.620,9 tons, and 1.505 tons were obtained from aquaculture. In addition to these, 3.924 tons of fish processing facilities per year, and 35 tons of fish meal and oil processing facilities per year are operated. 66% of sea fish caught in Sinop are anchovy (Engraulis encrasicolus ponticus), 16% sprat (Sprattus sprattus phalericus), 13% horse mackerel (Trachurus trachurus), 3% bluefish (Pomatomus saltatrix) and 2% consists of other species. The most preferred marine fish by the consumers are anchovy (Engraulis encrasicolus ponticus), horse mackerel (Trachurus trachurus), whiting (Merlangus merlangus), red mullet (Mullus barbatus), bluefish (Pomatomus saltatrix), turbot (Psetta maxima) and bonito (Sarda sarda) and sea bream (Sparus aurata), sea bass (Dicentrarchus labrax) and rainbow trout (Oncorhyncus mykiss) are the culture fish species offered for sale throughout the year.
Показать больше [+] Меньше [-]An Analysis of the Change in the Attitudes and Behaviors of Users before and after the Pandemic: Case Study; Ankara Çubuk-1 Dam Полный текст
2021
Sertaç Güngör | Elif Nur Doğan
During the pandemic process that has been going on for more than a year since the pandemic period was declared in 2020, the troubles and restrictions faced by people negatively affect the morale motivation of people. In this study, the definition of recreation areas, which are the leading places where people feel happy and peaceful, has been defined, what they contain is mentioned, and the adequacy of the recreational areas has been discussed in the survey and it has been determined that they are one of the most important needs in the cities. In the example of the recreation area of Çubuk-1 dam, it was investigated whether there is a change in the attitudes and behaviors of people before and after the pandemic, not face to face due to the pandemic, but through a voluntary survey based on social media. Although individuals thought that it was crowded after the pandemic, they preferred to use the recreation area of Çubuk-1 dam by paying attention to mask, distance and hygiene rules. The positive effects of physical activity, socialization, and outdoor travel on health are generally accepted by individuals. During the pandemic process, it has been observed that people have changed the way they spend their free time and the places they want to be, as they are overwhelmed by staying in their homes due to the prohibitions. The psychological and physical positive effects of recreation areas on human health and the changes caused by the pandemic in people's attitudes and behaviors paralleled the responses of the participants to the questionnaire.
Показать больше [+] Меньше [-]Effects of Environmentally Relevant Ammonium Nitrate Levels Caused by Agricultural Activities on Four Amphibian Species in The Eastern Black Sea Region Полный текст
2021
Handan Karaoglu
Within the scope of this research, the chronic effects of ammonium nitrate, which is the most widely used fertilizer worldwide, on the tadpoles of Marsh Frog (Pelophylax ridibundus), the Iranian Long-Legged Frog (Rana macrocnemis), the Caucasian Parsley Frog (Pelodytes caucasicus) and the Variable Green Toad (Bufotes variabilis) were examined. To assess the chronic effects of the fertilizer, the tadpoles of all the species were exposed to 0, 5, 10, 15, 20, 25 mg/L concentrations in same conditions. The chronic concentrations were applied from the 25th developmental stage to the 42nd developmental stage. It was defined at the end of the experiments that although there were some variability between different populations of the same species or between different species in the observed effects, chronic levels of ammonium nitrate caused decreased growth rate, prolonging in time to complete metamorphosis, increased abnormalities, and mortality in general. Among the 4 amphibian species, the Variable Green Toad was the most damaged one in terms of growth reduction (on average 31-41 %), abnormality rates (on average 50-75 %), prolonged time to complete metamorphose (14-21 days on average) and mortality rates (%54-100). The most adaptive species and the least damaged one was an Iranian long-legged frog for growth reduction (on average 0-15 %), prolonged time to complete metamorphose (7-9 days on average), and mortality rates (%9-15). All the harmful effects of chronic fertilizer levels caused by agricultural activities in the region had very important for examined species in our researches and it can be said that important environmental and biodiversity problems may occur if certain precautions are not taken regarding the use of the fertilizers and if the attitudes of the farmers on this issue cannot be changed.
Показать больше [+] Меньше [-]The Essential Oil Components of Helichrysum pallasii Flowers Полный текст
2021
Emine Yurteri | Fatih Seyis | Haydar Kuplemez
The genus Helichrysum contains many species displaying therapeutical effects and use in folk medicine. Helichrysum pallasii (Spreng.) Ledeb. is one of these species, used in the region for traditional medicine and ornamental purposes. Plants have been used in the treatment in folk medicines since the earliest times of human history. Chemical composition, antimicrobial and other medicinal properties of plants are being investigated in laboratories throughout the world using the extracts and essential oils derived from medicinal plants. Essential oils are usually complex mixtures of natural compounds of both polar and apolar structure. Essential oils consist mainly of terpenoids and their oxygenated derivatives. Essential oils, which have antioxidant and antimicrobial activities, have therapeutic properties and are the raw materials of cosmetics, as well as acting as natural additives in foods and food products. In this study, the volatile components of the H. pallasii plants were investigated which collected from its natural habitat in the Armutlu district of Bayburt province (Turkey) at the time of flowering. The proportion of essential oils of the plant harvested during the full flowering period and the aromatic components in the essential oil of the plant were determined using the SPME (Solid Phase Microextraction) method in a Gas Chromatography (GC-MS) device. Almost 37 different components were found as a result of the analysis. The main components were found as α-Springene (34.89%), (-)-Isoledene (7.11%), δ-Selinene (5.79%) and Aristolene (5.09).
Показать больше [+] Меньше [-]The Effect of Humic Acid Applications on the Development of Lilium Candidum Plant, Phenological and Pomological Observations in the Plant Полный текст
2021
Seda Bice Ataklı | Sezer Şahin | Onur Sefa Alkaç
In this study, a pot study was carried out to reveal the effects of direct and foliar applications of humic acid to the growing medium in addition to basic fertilization on the development and plant quality characteristics of lily plants grown in peat + perlite medium. The experiment was carried out in an unheated greenhouse during the summer, in a greenhouse environment with a shade net on it. In the experiment, in addition to the control, the humic acid doses were 3.5 lt/da from soil, 7.5 lt/da from soil, 1.75 lt/da from leaf, 3.75 lt/da from leaf and soil + 3.5 lt/da from leaf + 1, It was applied 3 times as 75 lt/da. In the pot experiment established in three replications according to the randomized plots trial design, 10 lily bulbs were planted in each pot and humic acid applications were made at two-week intervals following plant emergence. In order to see the effect of the applications when the plant is harvested, the length of the flower stem (mm), the thickness of the flower stem (mm), the fresh weight of the branch (mm), the number of buds (pieces), the bud length (cm), the number of leaves (pieces), Full bloom stem (mm) and vase life (days) were examined. As the doses of humic acid increased, the vegetative weight of the lily plant increased, while the effect of the applications on the investigated properties was different. With the mineral elements, hormones and plant growth regulators in humic acid, it has the feature of promoting the increase of biomass in the plant.
Показать больше [+] Меньше [-]The Use of Different Fat Sources on Performance, Egg Quality and Egg Yolk Fatty Acids Content in Laying Quails Полный текст
2021
Rabia Göçmen | Gülşah Kanbur | Yusuf Cufadar
In this study, the performance, egg quality, egg yolk colour and fatty acids profile of quails fed on diets containing different fat sources were determined. During 8 weeks trial, a total of 75, 10-weeks-old laying quails were used. Three diets were formulated to contain soybean oil (SBO), sunflower oil (SFO) and hempseed oil (HSO), respectively. The performance parameters were not significantly influenced by the dietary different oil sources. Eggshell ratio, eggshell thickness, eggshell breaking strength, egg shape index, egg yolk index, albumen index and egg yolk colour values (except a*) were not influenced by the different dietary oil sources. The a* value was significantly affected and the highest a* value was the HSO of group. The different oil sources supplementation to the diets was effective on fatty acid composition of the egg yolk. The highest value in terms of α-linolenic acid, total polyunsaturated fatty acids and total n-3 fatty acids were found in the diet fed group with HSO added. As a result; supplementation of different sources of oil to quail diets without negatively affecting performance and egg quality can be used to change the egg yolk fatty acid composition. Hempseed oil may increase the amount of total polyunsaturated fatty acids and total n-3 fatty acid content of egg yolk.
Показать больше [+] Меньше [-]Tıbbi ve Aromatik Bitkilerin Ekstraksiyon Yöntemleri, Gıdalarda Kullanımı ve Takviye Edici Gıda Alanında Değerlendirilmesi Полный текст
2021
Derya Arslan | Merve Aydın | Selman Türker
Tıbbi ve aromatik bitkiler (TAB), tüketicilerin doğal beslenme ve yaşama olan ilgisinin artmasıyla birlikte son yirmi yılda ön plana çıkmıştır. Türkiye, bulunduğu coğrafi konum, sahip olduğu iklim ve bitki çeşitliliği sayesinde TAB açısından önemli bir yere sahiptir. Oldukça geniş bir varyete gösteren TAB’ın; lezzet, koku, tat verici, antimikrobiyal, antioksidan ve terapötik gibi etkileri nedeniyle çeşitli formlarda (bütün, öğütülmüş, toz, ekstrakt, ekstre, hidrosol veya uçucu yağlar) kullanımları mevcuttur. Bileşimlerindeki sekonder metabolitlerden dolayı tıp ve eczacılık alanlarının yanı sıra fonksiyonel gıda bileşeni ve takviye edici gıda olarak kullanımları da önem taşımaktadır. Son yıllarda dünya ölçeğinde artarak yaşanan küreselleşme, kentleşme, değişen yaşam koşulları ve beslenme alışkanlarının farklılaşması ile birlikte ortaya çıkan yetersiz ve dengesiz beslenme sonucu tüketicilerin takviye edici gıdalara yönelimini artırmıştır. Takviye edici gıdalar pazarında TAB’ın kullanımına daha fazla yer verilmesiyle bu alanda gerekli düzenlemelere gidilmiştir. Bu derlemede, TAB ile bunların takviye edici gıda üretiminde kullanımı üzerine bazı bilgilere yer verilmiştir.
Показать больше [+] Меньше [-]Effect of Ferrous Gluconate on Chromosomal Abnormality Index of Allium Cepa Root Tip Полный текст
2021
Nergis Kaya
In completed research, ferrous gluconate -a food additive- used to preserve black color to prevent discoloration during storage in ripe black olives, and Allium cepa L. species. A. cepa L. roots were treated with different doses of ferrous gluconate. The effective concentration EC50 (0.068 g/l) was determined. A. cepa root tips were treated with EC50/2 (0.034 g/l), EC50 (0.068 g/l), 2XEC50 (0.136 g/l) dose for 24, 48, 72 hours, and afterward, the root tips were prepared for observation under the light microscope according to the method of preparing mitotic preparation. Chromosomal abnormality index (CAI) and genotoxic effect of ferrous gluconate in A. cepa root tip cells were determined. Repeated measurement ANOVA and TUKEY multiple comparison tests were used to investigate the effect of time and dose together on genotoxicity. C-mitosis, polyploidy, polar shifting in anaphase, polar shifting in telophase, equatorial plate shifting, laggard chromosome was observed by microscope. The highest CAI (70.16±4.85) was observed at 72h for 2XEC50 dose. Chromosomal aberration is also observed in control group. While the most common chromosomal aberration is determined as C-mitosis; The least observed chromosomal aberration is determined as polyploidy. Research results revealed that ferrous gluconate has a genotoxic effect on the root tip of A. cepa.
Показать больше [+] Меньше [-]Determination of Some Agronomic and Fruit Quality Characteristics of Some Watermelon Accessions from Turkish Watermelon Germplasm Полный текст
2021
Ercüment Atlı | İlknur Solmaz | Nebahat Sarı | Haşim Kelebek
This study has been conducted to determine the fruit quality parameters such as sugar and carotenoid content as well as plant and fruit characteristics of 11 local watermelon genotypes from watermelon genetic resources collection of Cukurova University, Department of Horticulture. First and 50% male and female flowering period, main stem length, main stem diameter, number of nodes on main stem, total yield, fruit weight, fruit length, fruit diameter, fruit rind thickness, total soluble solids (TSS), sugar and carotenoid contents were examined. Although there is no significant difference for main stem diameter and number of nodes on the main stem, significant differences were obtained for total yield, sugar and carotenoid composition of the genotypes. Carotenoid and sugar analysis were performed with a high-performance liquid chromatographic method coupled with diode-array detector (HPLC-DAD) and HPLC coupled with refractive index detector (RID), respectively. In all studied genotypes, cis-13-lycopene and β-carotene were the most abundant compounds. As expected for watermelon genotypes, the main sugar found in all studied genotypes was fructose. According to PCA analyses, genotypes were characterized by physical and chemical composition. Overall evaluation of results revealed that Kar 147 had better potential with carotenoid, sugar contents and fruit characteristics.
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