It is required to increase the amount of coagulant enzyme substitutes obtained from various sources that are equivalent to animal rennet in order to meet the rising demand for cheese. This study looked into the availability of plant-based enzymes as an alternative to rennet made from animals and microorganisms, which are frequently employed in the manufacturing of white cheese. Using animal (100% chymosin), microbial (obtained from Muchor miehei), and plant-based (Safflower seed, 80% chymosin-20 percent pepsin, purified from Cartamus tinctorius L.) enzymes, 6 varieties of raw and pasteurized milk cheese were made for this purpose. The values of SH, ripening index, water-soluble nitrogen (WSN), nitrogen dissolved in trichloroacetic acid (TCA), and yeast-mold increased after storage in cheeses made with various coagulant enzymes. During storage, the values for dry matter, pH, protein, Total Aerobic Mesophilic Bacteria (TAMB), lactobacilli, lactococci and coliforms all reduced. In terms of L*, a* and b* values, different rennet usage was discovered to be significant among cheese samples. The casein protein began to hydrolyze and the strength of the bands decreased in SDS-PAGE with the breakdown of the αs-casein and β-casein fractions on the 90th day of maturation in P1 and P4 numbered cheeses, which used plant-based enzyme made from raw and pasteurized milk. These changes in casein fractions resulted in a bitter taste in cheese made with plant-based enzymes. The use of plant-based enzymes in the manufacturing of white cheese was shown to produce results that were comparable to those of animal and microbial enzymes and did not have any negative effects on the cheese's physicochemical and microbiological quality parameters. When making fresh white cheese, the plant-based enzyme extracted from Carthamus tinctorius L. can be employed as a promising source of plant coagulants.

This study was carried out to determine the effects of different openings onto the polyethylene (PE) packages used in storage of ‘Sultani Çekirdeksiz’ grape variety on quality, SO2 damage, pathological and physiological disorders of grapes. Grapes were harvested at full maturity and placed in PE bags with ‰0 (control), ‰0.5, ‰1 and ‰2 openings. After pre-cooling grapes in the case of SO2 pets were placed after the mouth of the bags were closed. Grapes were stored at 0°C and 90-95% relative humidity for 4 months, and quality changes were determined in monthly samples. SO2 concentrations detected at the end of storage in PE packages with ‰0, ‰0.5, ‰1 and ‰2 openings were 11.16, 5.80, 2.05 and 0,00 mg/kg respectively. It has been found that grapes in packages without opening had significant SO2 content and SO2 damage, especially the end of storage. However, grapes in packages with ‰2 openings had high weight loss and browning scores in stems. The results showed that ‘Sultani Çekirdeksiz’ grapes could be stored more successfully in PE packages with ‰0.5 openings. ‘Sultani Seedless’ grape variety, it may be suggested to use PE bags without openings for 2 and 3 months storage.

The purpose of this study was to explore the possibilities of improving chilling stress tolerance of eggplant seedlings through exogenous melatonin (MEL) application. Eggplant (Hadrian F1) seedlings were treated with various concentrations (0, 1, 5 or 25 μM) of MEL via soil drench after which they were subjected to chilling stress at 5°C/10°C (night/day) for 3 days. Following stress imposition, the efficacy of MEL applications on enhancing chilling stress tolerance was determined by several physical and physiological measurements and biochemical analyses. The results demonstrated that exogenous application of MEL alleviated the adverse effects of chilling stress in eggplant seedlings. Among the MEL concentrations tested, 5 µM was determined as the most effective concentration since antioxidant enzyme (CAT, POX and APOX) and photosynthetic activities increased while visual and membrane damage decreased in 5 µM MEL-treated seedlings. Also, these results are the first experimental evidence that exogenous application of MEL could improve chilling stress tolerance in eggplant, but further detailed studies are necessary to better understand the mechanism in acquiring chilling tolerance.

Chestnut is an economically important tree because of wood and nut production, which is usually found in warm-temperate species, especially Mediterranean region. The fruit of chestnut is a good antioxidant source besides being rich in vitamin C. Anatolian chestnut (Castanea sativa Mill.) is a natural chestnut species in Türkiye. This study aimed to determine the most appropriate grafting time and grafting method for two chestnut cultivars (Marigoule and Erfelek) collected from the Western Black Sea region. Two different varieties (Marigoule and Erfelek) and three different grafting methods (chip budding, tongue, and cleft) were applied in different environments (in the hoop house and open field) for six months (December, January, February, March, April, and July). Analysis of variance was used for the grafting trials which was established and applied in the factorial trial design. Results showed that grafting success in the hoop house was twice as high in the open field, and the tongue was the most successful grafting method in all months except July. However, chip budding was observed as the most successful method in July. The highest grafting success was achieved in February, followed by July. According to the obtained results, Marigoule (C. crenata x C. sativa) cultivars were more successful than Erfelek cultivars. Marigoule cultivar’s success rate was 86% for the tongue method in February at hoop house. In July, 83% grafting success was achieved.

In this study, oat bran and blackberry were used separately and together in the production of probiotic yogurt (Lactobacillus acidophilus). It was aimed to determine the effect of oat bran and/or blackberry on probiotic viability, total phenolic content and antioxidant activity levels (DPPH, ABTS, CUPRAC methods) of yoghurts during both cold storage times and in vitro gastrointestinal digestion stages. At the end of the 21 day of storage period, it was determined that the highest and lowest L. acidophilus viability was found in yoghurt containing blackberry (B) (107 cfu/g) and oat bran (Y) (105 cfu/g), respectively. Oat bran and blackberry containing yogurt YB and control yogurt had the highest and lowest total phenolic content at the beginning and end of the storage period, respectively. According to the antioxidant activity results determined by the DPPH method, B yogurt had the highest antioxidant activity, while according to the ABTS and CUPRAC methods, YB yogurt had the highest antioxidant activity. As a result, it has been revealed that the functional properties of probiotic yogurt can be further improved with the addition of oat bran and blackberry, and blackberry can have a prebiotic effect on L. acidophilus.

Honey bees are a very important species in terms of economy, agriculture, and environment. In recent years, there has been a significant reduction in honey bee colonies in some parts of the world. Honeybee losses are not an unusual event, but there has been a significant reduction in honey bee colonies in many countries around the world. Due to the different social behaviors of honey bees, it is difficult to identify the main factors causing colony losses. According to the latest research, colony losses are mainly caused by parasites, diseases, bee keeping practices, and bee management including reproduction, changes in climatic conditions, agricultural practices and pesticide use, pesticides, nutrition, and beekeeping practices. In this review, the structure of prebiotic, probiotic, climate change, and vitollegen, which causes colony losses, is emphasized, and the potential solutions of these factors that will shed light on colony losses in honey bees from a different point of view are emphasized. In addition, bibliometric analysis was performed using the SCOPUS database to emphasize the importance of probiotic microorganisms and vitellogen.

Changing climatic conditions and growing population size lead take precautions against potential risks. Researchers who study in the agricultural field, including animal production, focus on finding out variants and breeds resistant to diseases and environmental stress. Detecting gene regions that affect resistance to diseases and environmental stress might increase the performance of the selection in favor of these traits. Due to these reasons in this study, five Turkish native cattle breeds were investigated for SNPs belonging to TLR2, NOD2, and IFNγ (using EcoRV, BsaHI ve HphI enzymes, respectively) reported to be associated with paratuberculosis previously. In total, 169 animals were analyzed from Native Southern Yellow (NSY= 29), East Anatolian Red (EAR=35), Anatolian Grey (AG=36), South Anatolian Red (SAR=34), and Native Black (NB=35). The genotype and allele frequencies were calculated with the PopGene32 program, and Khi square test was performed to determine whether the populations were in Hardy-Weinberg equilibrium (HWE). While at TLR2- EcoRV and NOD2- BsaHI loci, two alleles (A and C, C and T, respectively), IFNγ- HphI locus was found to be monomorphic for the G allele. For TLR2- EcoRV locus, the C allele was found as predominant except NB breed, and the C allele was predominant for all breeds at NOD2- BsaHI locus. However, in all breeds, NOD2- BsaHI locus was at HWE, for TLR2- EcoRV NSY and SAR breeds showed derivation from HWE.

This study was required to assess the existing tef market outlets and examine factors affecting market outlet choices among smallholder tef producers in western Ethiopia. For this study qualitative and quantitative data were collected from tef producers. Purposive and multi-stage sampling technique was used to determine sample households. The descriptive statistics and multivariate probit model were employed to analyze the collected data. The result shows that there are five alternative main market outlets available for the tef producers that are wholesalers, collectors, cooperatives, consumers, and retailers. These market outlets were affected by household’s demographic such as age, gender, education level, and household size), socio-economics (land allocated for tef, a variety used, livestock holding, and off/non-farm activities), institutional (credit, extension, and market information), and market (volume of tef produced, own transport facility, and nearest market) factors. Based on the findings strengthening farmers’ skills and knowledge through training, advising, and supervision; capacitate farmers by the additional work atmosphere and empowering women farmers by improved variety, working capital, and other recommendations are need attention by respective sectors.

This study was performed to investigate how to smoke solution affects dry matter loss, crude protein (CP) and toxic compound ODAP contents of grass pea in hydroponic media during seven days period. Three doses of smoke solutions (1, 5 and 10%, V: V) by diluting with tap water were applied to seeds as priming agent, and tap water was used as a control (0%). The experiment was arranged according to the split-plot design with three replications. Compared to seed, significant change was detected in hydroponic sprouts depending on the day in terms of dry matter loss and chemical content. Grass pea sprouts had higher CP and lower ODAP content than seed, even in control treatment. Furthermore, smoke solution increased the efficiency of system by increase CP, decrease ODAP content and also decreases dry matter loss, with significant effect on doses. CP of sprouts increased day by day and was the highest on day 6 (36.27%) at the 10% dose of smoke solution followed by 5% on day 7 (36.09%). ODAP decreased up to day 6 and then began to increase, but it was lower than control at all doses of solution during to experiment. This study showed that grass pea is suitable crop for hydroponic forage produce and that smoke solution can make a significant contribution to yield and quality of sprouts, especially at the dose of 10%. However, when the yield, nutritional value, and safe use were evaluated together, it was observed that the best time for harvesting was the end of the 6th day and delaying did not provide a significant advantage and even occurred undesirable results in terms of ODAP and dry matter.