Domuz ayrığı (Dactylis glomerata L.) yaygın olarak yetiştiriciliği yapılan, ekonomik açıdan önemli çok yıllık bir buğdaygil yem bitkisidir. Bu araştırma, Ege Tarımsal Araştırma Enstitüsü yem bitkileri ıslah programı kapsamında geliştirilen elit domuz ayrığı klonlarının akrabalık derecelerini ve genetik çeşitliliğini belirlemek amacıyla yürütülmüştür. Çalışmada 32 genotip ve 24 SSR primeri kullanılmıştır. Moleküler analiz sonucunda SSR primerleri toplamda 126 allel üretmiştir. Allel sayısı 3 ila 7 arasında değişmiş ve lokus başına düşen ortalama allel sayısı 5,25 olarak bulunmuştur. Elde edilen allel büyüklükleri ise 101 bp ile 354 bp arasında değişmiş ve polimorfizm oranı her primer için %100 olarak gerçekleşmiştir. Bireyler arasında uzaklık derecesi Jaccard genetik uzaklık katsayısı kullanılarak elde edilmiş ve 0,21 ile 0,84 arasında değişmiş ve genetik çeşitlilik seviyesi yüksek bulunmuştur. Genotiplerden elde edilen 126 allelin 28’nin nadir alleller olduğu ortaya çıkmıştır. Jaccard genetik uzaklık katsayısı kullanılarak yapılan neighbor-joining analizi sonucunda oluşturulan dendrogram 3 ana gruba ayrılmıştır. A grubu en büyük grubu oluşturmuş ve bünyesinde 15 genotip barındırmıştır. B grubu orijini aynı bölge olan 13 genotiple ikinci büyük grubu oluşturmuştur. C grubu ise en küçük grup olup orijini Türkiye’nin kuzeyi olan genotipleri barındırmıştır. Moleküler analizler domuz ayrığı genotiplerinin önemli derecede genetik varyasyon taşıdığı ve ıslah programı için değerli kaynaklar olduğu ortaya çıkmıştır. Bunlara ek olarak, SSR tekniğinin domuz ayrığı genotiplerini moleküler olarak tanımlamada oldukça uygun ve etkili bir teknik olduğu sonucuna varılmıştır.

Listeria monocytogenes is a food pathogen responsible for listeriosis, a relevant disease in public health worldwide. The genus Listeria spp., corresponds to cosmopolitan bacteria and capable of surviving different adverse conditions, which increases the risk for the food to be contaminated at any stage of the food chain. Fish and fish products are foods of high production level and, due to their chemical or nutritional composition, are highly susceptible to deterioration and contamination by pathogens in their productive chain relating to cases of listeriosis. Derived from the incidence and human mortality due to causative agents of listeriosis, along with their resistance to antimicrobials, they have acquired a greater emphasis on human health, animal health and food industry, resulting in the implementation of safety systems such as good hygiene practices, Hazard Analysis and Critical Control Points (HACCP) system, analytical methods and microbiological criteria, as some of the actions to contribute to the food safety and public health protection. The purpose of this review document is to provide, in a general way, aspects involved in foodborne illnesses, specifically listeriosis and its association with fish as a transmitting food, considering the prevention and control measures of this disease through food. It also includes aspects related to antimicrobial resistance by bacterial isolates obtained from fish, their implications and health risks.

Herbal medicine also called botanical or phyto-medicine refers to using a plant's seeds, berries, roots, leaves, bark, or flowers for medicinal purposes. It is becoming more main stream as improvements in analysis and quality control along with advances in clinical research show the value of herbal medicine in the treating and preventing disease. This study was focused on a traditionally prepared and used a product consist of an oil complex (olive oil, Nigella sativa seed oil, rosemary oil); which were researched antimicrobial effect of the product alone, and each oil by disc diffusion methods. Gram-positive and -negative bacteria and yeasts were employed in the susceptibility tests. The antibiotics of imipenem and vancomycin were exploited as positive controls. While there wasn’t effect on Gram-negative bacteria, strong antimicrobial effects were observed on Gram-positive bacteria and yeasts. Nigella sativa seed oil was the most effective. There wasn’t antagonistic and/or synergetic effect for the oil complex product. Although essential oils may be assigned the same name, they may differ widely as antimicrobial agents due to the extraction method used, which may explain the distinctive antimicrobial results reported in different studies. This was the first study to investigate the antibacterial and antifungal effects of this herbal medicine.

The aims of this study were to evaluate the traits of reproductive, milking and growth performance of Saanen and Saanen × Hair crossbreds (F1, B1 and B2) raised at the Bandırma Sheep Research Institute. The data were collected from 868 goats for reproductive performance and milk yield and from 1077 kids for survival performance between 2009-2012. Milk components (fat, protein, lactose and non-fat dry matter) of goats (n=162) and body weight changes of kid’s (n=64) were evaluated for one year in 2012. Estrus rates and survival rates were similar among all genotypes. Kidding rate was significantly higher in F1 goats than Saanen goats. Saanen goat had the lowest average mean daily milk yield compared with other genotypes. The effect of year and age were significant on milk yield. Goats in F1 and B1 genotypes had higher milk protein percentage and non-fat dry matter content in comparison with Saanen and B2 genotype. Milk fat and lactose contents were similar among genotypes. Birth weight and monthly live weight were similar between genotypes while weaning weight, six month live weight and yearling live weight were higher in B1 kids in comparison with Saanen kids. The effect of dam age and birth type were significant on birth weight, monthly live weight and weaning weight. The findings related to performance characteristics in goat genotypes investigated show that B1 genotype could be recommend for semi-intensive production system in the Western Anatolia.

The aim of this study is to determine the effect of zinc (Zn), which is a common nutritional problem for both plant and human health, on corn and wheat plants. In the research carried out in greenhouse conditions, Zn (Zn0: 0 mg Zn kg-1, Zn5: 5 mg Zn kg-1) was applied to Çukurova and Niğde soils having a natural Zn deficiency and yield and mineral nutrient concentrations of wheat and corn plants were determined. Shoot dry matter yields of wheat and corn plants in both soils significant increased by Zn application. Zinc application caused to 20 and 76% shoot dry matter yield increase for wheat in Çukurova and Niğde soils. The increase in corn pants were greater which was 174% in Çukurova soil and 127% in Niğde soil. The Zn concentration was significantly increased with the increased shoot dry matter yield, while shoot Fe, Mn and Cu concentrations were decreased with Zn applications.

Fusarium graminearum species complex is the major Fusarium head blight disease pathogen in worldwide. Fusarium head blight disease lead to damages on small grain cereals. The identification of the F. graminearum species complex is important for improving disease control and management. Traditional identification methods such as macroscopy and microscopy analysis need to be supported by biochemical and genetics assays. Thus, DNA sequencing-based methods is one of the most preferred, reliable, low priced in the identification methods. In the study, 54 F. graminearum isolates obtained from diseased wheat, barley and corn fields in Turkey and Iran were identified by morphological characteristics and then characterized by species-specific SCAR marker. β-tubulin, Tef1-α, 28s rDNA and Histone H3 genes amplified, purified and then sequenced. The merged multiloci length was obtained as last of all 2215 bp. These sequencing results was used to multiloci genotyping assays. Last of all, 20 isolates were determined as F. graminearum sensu stricto by multiloci genotyping analysis. Remaining isolates were identified as F. asiaticum or Fusarium sp. . The findings are important in terms of revealing the first-time identification in Turkish and Iranian isolates as F. graminearum species complex members by amplification four (β-tubulin, Tef1-α, 28s rDNA and Histone H3) highly conserved DNA regions.

This study was conducted in Kumkale Plain of Çanakkale Province. In this research, it is aimed to determine which factors the regional farmers take into consideration in their production and the costs of the products they obtain. The data of the study consisted of primary and original questionnaires provided through face-to-face interviews. The sample volume was determined by stratified sampling method and 99 questionnaires were conducted. The enterprises are ranked according to land size and divided into three layers as 1-50, 51-100, 101 decare and above. Analytical Hierarchy Process (AHS) method was used to determine producer preferences as well as simple cost calculations. The criteria of the study were determined as mechanization, irrigation facilities, supply of inputs, marketing opportunities, aquaculture information, product prices, input prices and labor force. According to the results of AHS obtained from the study, wheat ranked first with 0.213 points and sunflower ranked second with 0.196 points. The remaining alternatives are paddy, tomato, corn (seed) and silage maize respectively. According to AHS results, wheat, sunflower, paddy, tomato, corn and silage maize are preferred. Product costs are calculated as follows; 0.52 TL/kg for tomatoes, 1.56 TL/kg for paddy , 1.16 TL/kg for grain corn, 0.14 TL/kg for silage maize, 0.69 TL/kg for wheat and 1.44 TL/kg for sunflower.

Bu çalışmada, Reseda lutea L. (Resedaceae), Salvia absconditiflora Greuter & Burdet. (Lamiaceae), Thymus leucostomus Hausskn. & Velen. (Lamiaceae), Chorispora purpurascens (Banks & Sol.) Eig (Brassicaceae), Isatis floribunda Boiss. ex Bornm. (Brassicaceae), Linum tenuifolium L. (Linaceae) bitkilerinden elde edilen methanol ekstraktlarının Tribolium confusum Duv. (Col.: Tenebrionidae) ve Rhyzopertha dominica F. (Col.: Bostrichidae) üzerindeki toksik etkileri laboratuvar koşullarında test edilmiştir. Bitkilerden elde edilen methanol ekstraktları zararlıların erginlerine micro-aplicator yardımıyla uygulanmıştır. Yapılan tek doz (%10 (w/v)) etki çalışması sonucunda T. confusum (%23,17) ve R. dominica (%18,35)’ya karşı en yüksek kontakt toksisiteyi C. purpurascens bitkisinden elde edilen ekstrakt göstermiştir.

Ülkemizde mısır üretimindeki artış bir yandan sürerken, bir yandan da dünyada mısırın soyadan sonra en çok genetiği değiştirilmiş ürün olması nedeniyle tüketicinin mısır ile ilgili algısı her geçen gün daha olumsuz olmaktadır. Genetiği değiştirilmiş mısırın potansiyel olumsuz etkileri tartışılmakta iken, mısırda insan sağlığı için gözden kaçmaması gereken en büyük tehdit mikotoksinlerdir. Bu çalışmada, Türkiye’nin 24 ilinde bulunan 552 köyün 634 tarlasından toplanan mısır numuneleri GDO, aflatoksin B1, toplam aflatoksin, fumonisin B1, fumonisin B2, T-2 toksin, HT-2 toksin, zearalenon ve deoksinivalenol yönünden incelenmiştir. Tarama yapılan mısır numunelerinin hiçbirinde transgenik elemente rastlanmamıştır. Analiz edilen 634 numunenin sadece bir tanesinde Türk Gıda Kodeksi limitinin üzerinde aflatoksin B1 miktarına rastlanmış, numunelerin toplam aflatoksin değerleri Türk Gıda Kodeksi limitinin altında çıkmıştır. Paçal oluşturulan numunelerin hiçbirinde T-2 toksin, HT-2 toksin, zearalenon ve deoksinivalenol tespit edilmezken, fumonisin tespit edilen numunelerin toplam fumonisin miktarı Türk Gıda Kodeksi limitinin altında bulunmuştur. Bu sonuçlar, yerli üretim mısırların gıda güvenliği açısından uygun standartları sağladığını göstermektedir.