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Determination of Expression Level of Genes Associated with Anthocyanin Biosynthesis in Some Myrtle (Myrtus communis L) Genotypes
2022
Emine Açar | Yıldız Aka Kaçar
The Myrtle plant (Myrtus communis L.) is a plant species of the Myrtaceae family and a member of the maquis community, which naturally spreads in Mediterranean regions. Being resistant to arid conditions, the ecological distribution areas of the myrtle plant have been allowed to expand. The myrtle plant has been used of medicinal and aromatic plants, having interesting and beautiful flowers, and rich nutrient content of the fruit in terms of valuable phytochemicals, in particular, the nutritional content of its fruits and valuable metabolites have allowed the myrtle plant to be among the healthier foods. Antioxidant activity, which neutralizes reactive oxygen species (ROS), which causes many medical problems, is one of the most important features of the myrtle plant. Investigation of the biosynthesis of anthocyanin, which leads to antioxidant activity, and determination of the biosynthesis in different tissues and genotypes is important, especially in the development of production activities. Furthermore, this study aimed to investigate the anthocyanin biosynthesis in different genotypes with white and black fruits and various tissues of genotypes. For this purpose, the expression levels of CHS, CHI, F3H and PAL genes, which are involved in the anthocyanin biosynthesis pathway, were determined by qRT-PCR. In the study, it was determined that there was an increase in the level of genes related to the biosynthesis of anthocyanin in the leaf and fruit tissues of the genotype with white fruits. It was determined that the expression level of genes related to the biosynthesis of anthocyanin was observed to be higher in the leaf and fruit tissues of the genotype with black fruits, and the highest gene expression level was found in black fruits. It was observed that anthocyanin biosynthesis was synthesized in different tissues of the plant, and anthocyanin biosynthesis was higher in fruits compared to leaves.
Показать больше [+] Меньше [-]Chemical Constituents of Essential oil of Syringa vulgaris flowers
2022
Esma Nur Gecer
Medicinal plants gain a great interest in the drug development process due to their bioactive compounds content. Syringa vulgaris has been used as traditional medicine and it has considerable biological effects. In this study, essential oil (EO) was generated from Syringa vulgaris flowers by hydrodistillation and the chemical constituents were identified by GC/MS/MS analysis. The GC/MS/MS analysis revealed the presence of 57 compounds, and linalool (26.34%), α-terpineol (10.84%), trans geraniol (9.83%), α-bisabolol (4.50%), cis-nerol acetate (5.28%), lavandulyl acetate (4.32%) were found as major products.
Показать больше [+] Меньше [-]The Effect of Variety and Maturity on Quality Criteria and Sensory Properties of Hatay Olive Oil
2022
Gülçin Gündüz | Dilşat Bozdoğan Konuşkan
In this study, the quality criteria and sensory properties of olive oil samples of Saurani, Karamani and Halhali olive varieties collected in 3 different harvest times from Hatay province were investigated depending on the variety and harvest time. For this purpose, in olives; oil yield and ripening index, in the obtained olive oils; free fatty acids, peroxide number, UV specific absorbance and sensory properties were determined. The oil content of olives ranges between 23.77-34.77% and the highest oil yield was determined in the olive variety, Karamani (3rd maturity). In olive oils, free fatty acids were found in the range of 0.33-1.02% (oleic acid), K232 values ranging between 0.33-0.88, K270 values ranging between 0.004-0.177 and peroxide numbers between 2.47-8.40 meq O2/kg. The total phenolic content values of olive oils vary between 156.78-584.25 mg GAE/kg, and the highest phenolic content was determined during the first harvest of the Halhali variety. It was determined that the amount of phenolic content of the cultivars decreased with maturity. In the sensory analysis of olive oil samples, fruitiness was determined as greater than 0 by all panelists. The median of fruitiness in olive oil samples ranged from 3.62 to 5.88, and the median of fruitiness decreased with maturity in all varieties. The median of bitterness ranged from 2.62-5.23 and decreased with maturity. The pungency median varied in the range of 3.12-5.34, and pungency was reduced with maturity. The median of fruitiness, bitterness and pungency of Halhali olive oil was determined the highest.
Показать больше [+] Меньше [-]The Place of Goose Meat in Turkish Gastronomy
2022
Emel Çirişoğlu
Goose meat has always had a gastronomic value for both international and Turkish cuisines. The aim of the present study is to introduce the goose meat dishes that belongs to local Anatolian cuisines, to contribute to the recognition of the traditions of goose meat dishes, to record the goose meat dishes as a gastronomic value and to transfer these values to the next generations. According to the results of the present review there is not enough recordings for traditional goose meat dishes and goose meat is not commonly used in restaurant menus. Also, despite the presence of local traditions in which goose meat is at the center, whether these customs and activities are adequately promoted or not well announced. As a result, these problems should be examined in a wide range from breeding to marketing, and after solving the problems in goose breeding and eliminating technical deficiencies, it is recommended to carry out tourism activities to increase the recognition of the goose meat, which has an important gastronomic value for Turkish cuisine.
Показать больше [+] Меньше [-]Administration of Branched-Chain Amino Acids in the Pre- or Post-Hatch Period Improves the Fiber Characteristics of Pectoralis major Muscle in Turkey Poults Subjected to Early or Delayed Feeding
2022
Canan Kop-Bozbay | Nuh Ocak
Poultry meat quality is controlled by muscle fiber properties associated with body weight gain in the immediate pre- or post-hatch period. This study aimed to determine the effect of administration of branched-chain amino acids blend (BCAAb, 3 L-leucine:1 L-isoleucine:2 L-valine) in the pre- or post-hatch period on the growth performance and fiber characteristics of the Pectoralis major (PM) muscle in turkey poults subjected to early or delayed feeding. Newly hatched poults from eggs injected in ovo with BCAAb or received no injection were used in a 21-d study. Poults (n=192) produced without injection of BCAAb received a starter diet supplemented (BCAA) or not (C) with the BCAAb (2 g/kg) immediately or with a delay of 48 h (48BCAA and 48C) after hatching. Poults (n=36) produced with (IOBCAA) in ovo injection of BCAAb (2 mg /ml) received the diet and water immediately. The BCAA and IOBCAA poults had higher body weight than those of the C, 48C, and 48BCAA birds. The body weight of the C poults was higher than those of the 48C and 48BCAA birds. The BCAA poults had higher PM muscle weight than those of the C and 48C poults, while that of the IOBCAA poults was higher than that of the 48C poults. The IOBCAA treatment increased the protein content of the PM muscle meat, compared with the C and 48C treatments. The 48C treatment increased the type IIB fiber area and the type I and type IIA fiber percentages but decreased the type IIB fiber, compared with other treatments. The numbers of type IIB and total fibers in the IOBCAA poults were higher than those of the 48C birds. In conclusion, administering BCAAb in ovo or in a starter diet enhanced early growth performance and improved the fiber characteristics of the PM muscle in turkey poults.
Показать больше [+] Меньше [-]Vulnerability of Rice Farmers to Climate Change in Kwara State, Nigeria
2022
Sheu-Usman Oladipo Akanbi | Olanrewaju Solomon Olatunji | Olamide Sulaiman Oladipo | Uswat Temitayo Adeyemi | Akinyinka Akinyoade
Climate unpredictability and weather extremes are being projected as capable of presenting additional challenges for farmers currently engaged in the low-technology based food production systems in sub-Saharan countries like Nigeria. This study assessed rice farming households’ vulnerability to climate change in Kwara State, Nigeria. Primary data, collected from 150 respondents using simple random sampling procedure were analysed employing descriptive statistic was use to describe the coping strategies adopted and Human Development Index (HDI) was created to assess vulnerability of rice farmers to climate change. Statistical analyses indicated a vulnerability assessment index of 0.3001, pointing to a fact that the zone is prone to the adverse effects of climatic variability. For this reason, the study empirically underscores the need for farmers to adopt and adapt the planting of drought tolerant and/or early maturing varieties of rice. Importantly, the capacities of the local communities needs to be strengthened vis-à-vis the relationship between climate change and crop production. Capacity building at the farm level is crucial for improving crop, soil and water management, enhancing the demand for and use of better and more efficient production inputs. Tied to farm-level capacity building is the need to refocus public agricultural-based institutions towards exposing the rice farmers to effective mitigation strategies in the wake of climate change, provision of agricultural inputs, expansion of irrigation, efficient and effective extension service delivery, market development and other forms of necessary support.
Показать больше [+] Меньше [-]Current Approaches to Nitrite Reduction Methods in Meat Products and Their Potential for Transfer to Industry
2022
Gülen Yıldız Turp | Tuğçe Avcı
Nowadays, consumer demand for healthy and natural food is reflected in the meat industry and researches and investments on this issue have gained speed. Nitrite is a widely used synthetic additive in meat products due to its contribution to the development of characteristic color and flavor, controls lipid oxidation and has an antimicrobial effect on pathogenic microorganisms, especially Clostridium botulinum. However, the fact that nitrite causes the formation of toxic, mutagenic and carcinogenic N-nitrosamine compounds and constitutes a risk to human health has led to the searches for alternative additives. An important part of the studies on this subject consists of testing the use of natural additives as an alternative to nitrite. In recent years, instead of using nitrate and nitrite in meat products, studies have been carried out to use organic acids and microbial resources. In addition to these methods, up-to-date technology applications such as high pressure, irradiation, encapsulation, active packaging containing nitrite, zinc protoporphyrin IX compound formation and cold plasma technology constitute other research areas that we encounter in reducing the nitrite rate in meat products. Within the scope of this review, the functions of nitrite in cured meat products, current potential methods for reducing nitrite content and the limitations of transferring these methods to industry were examined.
Показать больше [+] Меньше [-]Phytoplasma: Transmission, Disease Development and Functional Genomics
2022
Zehra Mezreli
In recent years the number of phytoplasma diseases that negatively affect yield and quality of many plants has been increasing around the world. As is known grafting, plant propagation materials (seed, bulb, tuber), parasitic plants and insect vectors play active role in the spread of phytoplasmas causing these diseases. These organasim which get through plate in the sieve tube element and systemically colonize the plant, it invade and multiply salivary glands, midgut, heomocel, brain and fat tissue after sucking up insect body with stylet. Subsequently they continue the cycle by moving to a new plant with a diseased insect feeding on a healthy plant. Although the existence of functional genomics in this cycle is known, there is little information about transmission of phytoplasma from vector to host plant or from host plant to vector, the mechanism of disease caused by phytoplasma in the plant and genomics involved in different reactions in the host plant against phytoplasmal infection.
Показать больше [+] Меньше [-]The Effect of Roasting on Volatile Compounds of Ground Coffee and Turkish Coffee Brew
2022
Ceyda Dadalı
Roasting is the most important coffee processing stage that affects coffee flavour. Coffee brew is prepared by various methods and Turkish coffee brewing technique is one of the oldest methods among other coffee preparation techniques. In this study, volatile compounds of light, medium, dark roasted ground coffee and Turkish coffee brews prepared from light, medium, dark roasted coffees were evaluated and the effect of roasting process was determined. The volatile compounds from acid (2), alcohol (1), aldehyde (2), furan (8), furanone (3), ketone (1), lactone (1), phenol (3), pyrazine (19), pyridine (1), pyrrole (6), and thiophene (1) chemical group was detected in all roasted ground coffee samples. However, volatiles compounds from acid, alcohol and furanone group were not detected in any Turkish coffee brew. Pyrazines were main volatile compounds of light roasted ground coffee and Turkish coffee brew of light roasted coffee. On the other hand, furans were main volatile compounds of medium, dark roasted ground coffee and Turkish coffee brew prepared from medium, dark roasted coffees followed by pyrazines.
Показать больше [+] Меньше [-]Analysis of Clade V MLO Gene Expressions in Hazelnut Leaves upon Exposure to Powdery Mildew
2022
Ulku Baykal | Kadriye Özcan
Powdery mildew affecting European hazelnut Corylus avellana L. trees in Turkey is caused by the obligate biotrophic fungus Erysiphe corylacearum. This fungal disease causes significant economic losses by reducing the yield and quality of hazelnuts. Loss-of-function mutations in the mildew resistance locus o (MLO) gene family of many plants confer high levels of broad-spectrum resistance to powdery mildew. The proteins encoded by the genes at the MLO locus are divided into approximately seven different conserved clades. Among them, the phylogenetic clade V has been shown to be involved in PM susceptibility, as inactivation of these genes leads to long-term disease resistance in dicotyledons. In this study, we examined the temporal expression pattern of three hazelnut MLO genes, previously identified as clade V, in response to powdery mildew infection in C. avellana cv. Tombul. Leaves are the main tissue affected by the powdery mildew pathogen in hazelnut plants. Analysis of MLO expression in hazelnut leaves showed that CavMLO2 and CavMLO6 were significantly upregulated after challenge with E. corylacearum, providing preliminary evidence that they may be involved in PM susceptibility. Thus, these results present a basis for the isolation and use of relevant genes in plant breeding for disease resistance. Moreover, expression profiles of the clade V MLO genes are also important to identify candidate genes that need to be silenced or edited for future molecular studies to obtain resistant hazelnut varieties.
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