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The Detection and Enumeration of Coliform Bacteria in Some Ready-to-Eat Unpackaged Food in Fethiye Region
2021
Mehtap Çiftçi | Nilgün Öncül
Since coliform bacteria are common both in the intestine and in nature (soil, plant, etc.), they are considered as a sanitation indicator in the food industry. It is known that the majority of bacteria defined as fecal coliform in the coliform group are Escherichia coli. The presence of E. coli or fecal coliform bacteria in any sample is an indication that the necessary hygienic measures are not taken during production, storage, and sale. That means the sample is directly or indirectly contaminated with faeces, and/or other intestinal pathogens may also exist. In this study, raw milk, freshly squeezed fruit juices, unpackaged ice cream, shaved ice, and ice-cold samples were purchased from the famous touristic destination Fethiye and analyzed for coliform bacteria. For this purpose, the samples were purchased from local marketplaces, buffets, cafes, patisseries, restaurants, and roadsides at Fethiye. In total 60 samples were analyzed using Violet Red Bile (VRB) Agar. The results of coliform bacteria ranged
Показать больше [+] Меньше [-]Determination of the Antagonistic Effects of Some Rhizospheric Bacteria against Macrophomina phaseolina under In Vitro Conditions
2021
Özden Salman | Fatma Rana Bayram | Nuh Boyraz | Raziye Koçak
Macrophomina phaseolina (Tassi) Goid. is a fungal pathogen causes charcoal rot disease (Sin: Rhizoctonia bataticola) and is responsible for significant yield losses in many plants. In our study, we aimed to evaluate the antagonistic ability of 39 different bacteria, isolated from the fields of sugar beet in 2019, against the pathogen Macrophomina phaseolina isolated from sugar beet, beans and chickpeas. Approximately 31% of the bacteria showed antibiosis effect against the pathogen. It was determined that the effectiveness level of Lelliottia amnigena, Bacillus atrophaeus, B.pumilus and B. cereus (7 isolates) was moderate to high against Macrophomina phaseolina. Bacillus atrophaeus (PTo15-1a) showed the highest efficacy of 80%, 72.94% and 82.35% against Macrophomina phaseolina of chickpea, bean and sugar beet respectively. Lelliottia amnigena (Pto 14-1b) was moderately effective (57.78%) against the chickpea isolate of the pathogen. It was observed that of the seven Bacillus cereus isolates used in the experiment, three isolates (Pto14-1a, Pto12-1b, Pto17-1b) were highly effective against the chickpea pathogen, two (Pto12-1b, Pto14-2b) against bean pathogen, and one (Pto15-1b) against sugar beet isolate. Results have shown varied level of antagonism by different test bacterial against different Macrophomina phaseolina isolates, while the highest level of antibiosis shown by Bacillus atrophaeus against all pathogenic isolates indicated that it can be a potential future bioagent in managing the disease.
Показать больше [+] Меньше [-]In Vitro Regeneration of Tea (Camellia sinensis (L). O. Kuntze) By Somatic Embryogenesis from Immature Cotyledon Tissues
2021
Emine Yurteri | Mücahit Salih Can | Fatih Seyis | Haydar Kuplemez
Tea (Camellia sinensis) is the world's most popular beverage plant, as well as an important plantation crop with high commercial value. It has been maintained for centuries through conventional vegetative propagation. Tea clonal propagation in vitro has the advantage of producing a large number of elite plants. If an efficient in vitro regeneration technology is available, this technique could be exploited for selection of tea plants for desired trait. The selected plants could be later on multiplied through in vitro or ex vitro techniques. The study aimed to induced somatic embryogenesis from immature embryo explants to genetic variaton. Different concentrations of phenylboronic acid with benzyladenine and phenylboronic acid with kinetin were tested in MS medium with 30 g/L sucrose and 8 g/L agar. MS medium without any plant growth regulators was used as control group. Considering the embryo survival rate, 1.5 mg/ L-1 phenylboronic acid + 1 mg/ L-1 kinetin produced highest result as 87.3% while lowest was in control group as 36.7%. The highest plant regeneration rate was found in 1,5 mg/ L-1 phenylboronic acid + 1 mg/ L-1 kinetin and 1.5 mg/ L-1 phenylboronic acid + 1 mg/ L-1 benzyladenine medium respectively as 58.3% and 55.6%. Kinetin treatment with increasing phenylboronic acid concentrations gave the best results in terms of somatic embryo survival rate. Also, kinetin treatment produced better results when compared to benzyladenine concentrations.
Показать больше [+] Меньше [-]The Comparison of Different Honey Bee Genotypes by Some Biochemical Parameters (Total Protein, Total RNA, Catalase and Malondialdehyde)
2021
Adnan Ünalan | Ethem Akyol
In this study, some biochemical characteristics (total protein, total RNA, Catalase: CAT enzyme activity and malondialdehyde: MDA level) of Italian bee (A. m. ligustica) and Caucasian bee (A. m. caucasica), and Muğla and Anatolian bees (A. m. anatolica) from local honey bee races were investigated comparatively. Laboratory analyzes of biochemical characteristics were performed on worker bees aged 24 days old with 10 repetitions using appropriate methods informed in the literature. The amounts of total protein of bee races given above were 18.39±1.28, 20.71±0.63, 18.56±1.24 and 20.95±2.15 g/dL, respectively; the amounts of total RNA were 11.46±0.18, 12.10±0.26, 11.87±0.20 and 12.27±0.26 µg/µL, respectively; the CAT activities were 4.59±0.46, 5.12±0.67, 4.88±0.48 and 5.25±0.53 kU/g P, respectively; the levels of MDA were 0.52±0.04, 0.50±0.04, 0.48±0.02 ve 0.43±0.05 mmol/mg, respectively. Variance analysis showed that statistically significant differences among races in terms of the all characteristics examined. The results of CAT activity which is one of the indicators of antioxidant defense system, and levels of MDA which is an indicator of peroxidation of membrane lipids; and similarly total amount of protein also includes various proteins such as antioxidants and enzymes; it can be said that the Anatolian and Caucasian bee races (due to higher total protein, total RNA and CAT activities, and lower MDA level) are more resistant to various negative environmental factors (e.g. climate, flora, pesticide, etc.) than the Muğla and Italian bee races in the conditions of the Central Anatolia Region; there are significant differences between the bee races in terms of amounts of total RNA and this parameter can be also used in the characterization of bee races.
Показать больше [+] Меньше [-]Kurutma Yöntemlerinin Deveci Armudunun Kurutma Kinetiği ve Renk Parametreleri Üzerine Etkisi
2021
Ilknur Alibas | Aslıhan Yılmaz | Seda Günaydın | Begüm Arkain
Kuru baza göre başlangıç nemi %83,95 ± 0,01 y.b. (5,24 ± 0,003 kg su kg KM-1) olan 100 ± 0,10 g ağırlığındaki Deveci armudu dilimleri (Pyrus communis L. cv. Deveci) son nemi %11,40 ± 0,06 y.b. (0,13 ± 0,001 kg su kg KM-1) değerine ulaşıncaya dek gölgede kurutma, 60, 80 ve 100°C’de sıcak havayla kurutma yöntemleriyle kurutulmuş ve bu yöntemlerin kurutma süreçleri sırasıyla 11150, 437, 252 ve 148 dakikada tamamlanmıştır. Çalışmada deneysel olarak elde edilen zamana bağlı ayrılabilir nem oranı değerleri yirmi farklı ince tabaka kurutma eşitliği kullanılarak modellenmiştir. Buna göre 60°C ve 100°C için deneysel verilere en yakın sonuçları veren modelin Modified Henderson & Pabis eşitliği olduğu; buna karşın gölgede kurutma ve 80°C’de kurutma yöntemlerinde ise sırasıyla Alibas eşitliğinin ve Jena & Das eşitliğinin en iyi modeller olduğu görülmüştür. Gölgede kurutma yönteminde her hangi bir enerji tüketimi olmamasına karşın bu yöntemin oldukça uzun olması ve ürünün kalite parametreleri üzerinde olumsuz etkilere yol açması gibi nedenler Deveci armudunun kurutulmasında gölgede kurutma yönteminin kullanışlı bir yöntem olmadığını ortaya koymuştur. Bununla birlikte toplam enerji tüketiminin kurutma sıcaklığının artmasıyla yükseldiği görülmüştür. Ayrıca sıcaklık artışının kalite parametrelerini de olumsuz etkilediği tespit edilmiştir. Kurutma süresi ve özgül enerji tüketimi gibi işletim parametrelerinin yanı sıra parlaklık, kırmızılık, sarılık, kroma, hue açısı, toplam renk değişimi ve kahverengileşme indeksi gibi kalite parametrelerinin taze ürüne oldukça yakın olmasından dolayı 60°C’de sıcak havayla kurutma yönteminin Deveci armudunun kurutulması için uygun bir yöntem olduğu görülmüştür.
Показать больше [+] Меньше [-]Determination of Grafting Adaptation of Some Chestnut (Castanea sativa Mill.) Genotypes with Marigoule (C. Sativa × C. Crenata) Cultivar
2021
Şemsettin Kulaç | Hatice Nihan Nayır
In this study, to reproduce natural chestnut genotypes, the fruit characteristics of the Düzce region determined by local people are good in a healthy way, compatible with Marigoule (C. sativa × C. crenata) hybrid varieties resistant to branch cancer and root rot was investigated. In this study, 24 different chestnut genotypes were used. As the grafting method, the most commonly used tongue grafting, split grafting, chip budding grafting, and side grafting method were used. All procedures related to grafting were carried out in the greenhouses of the Düzce University Faculty of Forestry. In this study, local genotypes that are compatible with Marigoule chestnut were determined. As a result, the native genotype, which showed the best adaptation to Marigoule seedlings, was 87.5% of Yalnızçam, and after that, 79.2% of the Ereğli Sefer genotype. The lowest fit showed Broken genotype with 15% and Akçakoca1genotype with 17.5%. Besides, the compatibility of foreign varieties Maraval, Marigoule, and Betizac were also investigated. As a result, Betizac had the highest compatibility with 95%, while Maraval had 67.5%. The most successful in the grafting methods applied was the side graft (74.2%) followed by tongue grafting (59.9%), splitting grafting (51.4%), and chip budding grafting (29.7%).
Показать больше [+] Меньше [-]Marka Otantikliğinin Satın Alma Niyeti Üzerindeki Etkisinde Marka Bağlılığının Aracılık Rolü: X Marka Gıda Tüketicileri Üzerine Bir Uygulama
2021
Ebru Onurlubaş | Remzi Altunışık
Bu çalışmanın amacı, marka otantikliğinin satın alma niyeti üzerindeki etkisinde marka bağlılığının aracılık rolünü tespit etmektedir. Bu bağlamda, İstanbul İlinde 18 yaşından büyük X marka gıda tüketicilerine kolayda örnekleme yöntemiyle yüz yüze 384 anket uygulanmıştır. Verilerin analizinde, SPSS 23 ve AMOS 20 programı kullanılmıştır. Veriler Cronbach Alpha Katsayısı yöntemi, Normallik testi, Açıklayıcı Faktör Analizi, Doğrulayıcı Faktör Analizi, Yapısal Eşitlik Modeli, Sobel test kullanılarak analiz edilmiştir. Araştırma sonucunda, marka otantikliğinin satın alma niyeti üzerindeki etkisinde marka bağlığının kısmen aracılık rolü olduğu belirlenmiştir. Ayrıca marka otantikliği boyutlarından olan güvenilirlik, orijinallik ve doğallığın marka bağlılığı üzerinde, marka bağlılığı, orijinallik ve doğallığın satın alma niyeti üzerinde anlamlı bir etkisi olduğu tespit edilmiştir.
Показать больше [+] Меньше [-]Areal Precipitation Estimation Using Satellite Derived Rainfall Data over an Irrigation Area
2021
Mehmet Ali Akgül | Hakan Aksu
The average precipitation on the irrigation field can be estimated from the Meteorology Observation Stations by using spatial interpolation methods such as Thiessen polygon and isohyetal curves. However, the fact that precipitation doesn't occur homogenous in spatial scales, spatial interpolation methodologies need a large number of meteorology stations for more accurate results. In recent years, remote sensing methods have diversified to estimate precipitation. In this study, performance of the satellite-based precipitation data was assessed to determine areal precipitation over an irrigation area. This study was conducted over left bank irrigation area located in the Çukurova Plain of Turkey. Relationship between CHIRPS satellite based on monthly precipitation data and 4 meteorology stations’ data were analyzed. Determination coefficients (R2) of the stations were found between 0.64 and 0.77, for point based comparison, R2 was calculated as 0.84 with Thiessen polygon method. It is concluded that the precipitation amount in the irrigated area can be estimated as accurately as classical methods such as Thiessen polygon with satellite-based precipitation data.
Показать больше [+] Меньше [-]Effect of Tillage Techniques on Depth, Furrow Slice and Water Retention in Maugo Smallholder Rice Scheme in Kenya
2021
Pius Kipchumba Cheboi | Clement Kiprotich Kiptum | Japheth O. Onyando
This study was conducted with the aim of determining the influence of tillage techniques on depth, furrow slice and water retention in Maugo Smallholder Rice Scheme in Kenya. Treatments were arranged in randomized complete block design with four replicates. Four tillage treatments were used. The first one was conventional ox plough practiced by farmers where they first flood the field with water before ploughing. The other three treatments were ox plough, hand hoe and tractor ploughing all with no flooding before ploughing. Data collected included depth of ploughing and harrowing during land preparation. Furrow slices during ploughing and harrowing as well as water retention were recorded. Tractor ploughing had the highest mean depth of ploughing of 42.00 ± 0.81 cm followed by conventional ox ploughing with 17.75 ± 0.75 cm, ox ploughing15.75 ± 0.62 cm and hand hoe ploughing had the lowest mean depth 15.50 ± 0.28 cm. Tractor ploughing had the largest mean furrow size of 62.00 ± 0.91cm followed by conventional ox ploughing 32.25 ± 0.85 cm, ox ploughing 30.25 ± 0.85 cm while hand hoe ploughing had the smallest mean furrow slice of 16.5 ± 0.50 cm. Highest mean of retained water was recorded in week 4 in paddy rice fields prepared using conventional ox ploughing (10.5 cm), ox ploughing (10 cm), hand hoe ploughing (11.5 cm) and tractor ploughing (11.5 cm) while the lowest was recorded in week 15 for conventional plots. There were significant differences in mean depths during both ploughing and harrowing. Tractor ploughing mean depths were significantly different from the other treatments. The weekly mean water depths retained in the plots were more than 6 cm for the entire growing period of rice.
Показать больше [+] Меньше [-]An Important Genotype for Sustainable Extensive Goat Production Systems of Turkey: The Gökçeada Goat
2021
Cemil Tölü | Türker Savaş
In this study, it was aimed to report some traits of Gökçeada goats, which raised under the natural conditions of Gökçeada, with respect to their protection within the scope of genetic resources. In this respect the information obtained from the goats supplied from the Island and reared in a semi-intensive system at Çanakkale Onsekiz Mart University and also the information obtained from the literature were collected and the biological and zootechnical identifications of the Gökçeada goat were performed. Gökçeada goats, which are predominantly black, have yellow or red blazes on both sides of their heads including their eyes. It was seen that Gökçeada goats had similar body measurements and lower live weights as compared to those of the same species on the mainland. The birth weight and the mature live weight of Gökçeada goats were found as 2.55 kg and 38 kg, respectively, whereas their mean kid yield per goat at birth was determined as 1.6 to 1.8 kids. Individuals with a milk yield of 591 kg were striking in the Gökçeada genotype, in which the mean lactation length and the mean lactation milk yield were determined as 251 to 259 days and 227 to 245 kg, respectively. Its milk fat (4.92-5.75%) and milk protein (3.29%) resembled those of our other native breeds. It is necessary to reveal, with more elaborate studies, the potential for Gökçeada goat cheese that is greatly demanded on the Island. It was determined that the Gökçeada kids, which were considered as dairy kids, had some small but nonfat carcass and that their meat was soft and of a light color and had a slight smell. One should be careful about the adaptation of the Gökçeada genotype, which successfully maintains itself under the island conditions and which is the source of income for producers, to intensive and extensive goat production systems.
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