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Electrophysiologic and behavioral responses mediated by volatiles involved in the repellency of Apis mellifera (Lepeletier) (Hymenoptera: Apidae)
2020
Patricia Daniela da Silva Pires | Josué Sant'Ana | Ricardo Bisotto-de-Oliveira
Repellent volatiles to insects might be an important tool for management of bees in areas which the presence of these organisms is not required. This study aimed to evaluate the electroantennal and behavioral responses of Africanized honeybees (workers), Apis mellifera (Lepeletier) (Hymenoptera Apidae), at different ages, to benzaldehyde (BA) and methyl anthranilate (MA) and to evaluate the potential repellency of these compounds under field conditions. Laboratory tests were conducted to study electroantennographic responses (mV) and chemotactic behavior of worker bees aged 1-5 (young) and 20-30 (old) days in four choice olfactometer. Electrophysiological responses to each compound did not differ between young and old workers. Bees antennae (young) triggered significantly greater responses to BA, in the older ones, a higher response was observed to MA, both compared to control (ethanol). The threshold response to BA and MA was achieved at 10 µg/µL, both compounds repelled bees at the same dose in olfactometer. Treatments with BA and MA, in field conditions, were less visited by scouter honey bees than those without these compounds (control).
Показать больше [+] Меньше [-]Efficiency of botanical extracts against Aphis craccivora Koch (Hemiptera: Aphididae) nymphs in Vigna unguiculata (L.) Walp
2020
Jefferson Auteliano Carvalho Dutra | Victor Emmanuel de Vasconcelos Gomes | Ervino Bleicher | Deivielison Ximenes Siqueira Macedo | Mirla Maria Mesquita Almeida
The present study aimed to evaluate the insecticidal activity of hydroalcoholic plant extracts on Aphis craccivora Koch nymphs in cowpea. The experiments were carried out under greenhouse conditions in a randomized block design with five repetitions. Hydrated ethanol was used as a solvent in the botanical extract preparation. Cowpea plants were infested with five female adult aphids, eleven days after planting. After 48 hours, the adults were removed from the plants, leaving the recently bred nymphs. The evaluation of the nymphs’ survival was carried out forty-eight 48 hours after the application of the plant extracts. The botanical extracts with more than 50% efficiency were: Allium tuberosum leaf, Caesalpinia ferrea leaf, Piper aduncum leaf, Carica papaya seed, Dieffenbachia picta leaf, Cucurbita moschata seed and the control treatment, Annona squamosa seed. The botanical extracts with less than 50% efficiency were: Allium sativum bulb, Anacardium occidentale leaf, Borreria verticillata leaf plus flower, Coffea arabica leaf and seed, Delonix regia leaf and flower, Euphorbia tirucalli leaf, Leucaena leucocephala leaf, Mimosa caesalpiniaefolia leaf, Nerium oleander leaf, Syzygium cumini leaf, endocarp and pericarp, Syzygium malaccense leaf, Zingiber officinale rizome and Ziziphus joazeiro leaf. The highest efficiency levels were obtained by D. picta and C. moschata, rendering these extracts suitable for field trials to further evaluate their efficieny against the cowpea black aphid.
Показать больше [+] Меньше [-]Post-fire recovery of arthropod assemblage in an area of Brazilian savanna
2020
Hélida Ferreira da Cunha | Werther Pereira Ramalho | Amanda Martins Dias | Brenda Romeiro Peixoto | Gabriel Sampaio Jesus | Jennifer de Paula Oliveira | Thamara Missel Pereira da Silva
Fire is a frequent agent of disturbance in tropical savannas (e.g., Brazilian Cerrado), but relatively few studies have analyzed how the arthropod community responds to fire disturbance. Following the incursion of an accidental fire into a Cerrado fragment in Central Brazil, we investigated whether the arthropod community is structured by abiotic (climate or fire) or biotic (succession) factors. Our study commenced one week after fire and during the six months afterward. We found 22 arthropod orders, of which Diptera, Hymenoptera, Hemiptera, Blattaria and Coleoptera were the most representative. More than 40% of the arthropod abundance was recorded 40 days after the fire event. The overall arthropod abundance and richness fluctuated in the six months following the fire and does not seem to be related to climatic variables. Temporal beta diversity was explained by a reduction in richness differences along the intervals of time, but the community recovery needs to be treated with caution. The increase in replacement in the last intervals in relation to the fire event indicates that biotic interactions may occur with the arrival of late colonizers and suggest that arthropod communities need a long time to be restructured. These results indicate that the processes of restructuring of the arthropod communities after human-induced fire events are temporally complex, involving loss, gain and taxon replacement, but long-term studies are still needed to understand the dynamics of communities.
Показать больше [+] Меньше [-]New records of Sarcophagidae (Insecta: Diptera) collected in Cerrado fragments in the municipality of Campo Grande, Mato Grosso do Sul state, Brazil
2020
Ronaldo Toma | Wilson Werner Koller | Cátia Antunes Mello-Patiu | Ramon Luciano Mello
Collections carried out for a period of 10 weeks from October to December 2013 in two fragments of Cerrado (experimental farm of Embrapa Gado de Corte and Private Reserve of Natural Heritage belong to the Universidade Federal de Mato Grosso do Sul (RPPN-UFMS)) located in the municipality of Campo Grande, state of Mato Grosso do Sul, Midwestern Brazil, with traps baited with decomposing beef liver, and collections conducted for a period of 15 days in January 2014 in the RPPN-UFMS, using Shannon traps baited with dog corpses, resulted in 32 flesh fly species of eight genera, with the first record of the genus Blaesoxipha and 15 new species records to Mato Grosso do Sul.
Показать больше [+] Меньше [-]Egg parasitoids of the cassava hornworm (Erinnyis spp.) associated to cassava in the Pará State, Brazil
2020
Aloyséia Cristina da Silva Noronha | Dimison Garcia Blanco | Valmir Antonio Costa | Ranyse Barbosa Querino | Dênmora Gomes de Araújo | Norman F. Johnson
The aim of this study was to assess the occurrence and identify the egg-parasitoid species of the cassava hornworm (Erinnyis spp.) in cassava plants (Manihot esculenta Crantz - Euphorbiaceae), facilitating their usage in the biological control as a management strategy in the Pará State. During February to December of 2016, cassava hornworm eggs were collected in the Cassava Germplasm Bank area of the Embrapa Amazônia Oriental, located at the city of Belém, State of Pará. In the laboratory, eggs were separated in viable and parasitized and were daily observed until the hatching of the caterpillars and emergence of the parasitoids. The caterpillars were reared until reaching the adult stage and the sex determined at the pupae stage. A total of 482 eggs were collected, with a higher occurrence at March, and 244 caterpillars were obtained with sex ratio of 0.52 with 191 specimens reaching adult stage. Among these adults, 95.81% were Erinnyis ello (Linnaeus) and 4.19% Erinnyis alope (Drury). A total of 1,087 parasitoids were obtained from 131 eggs and belong to four Hymenoptera families: Platygastridae (Telenomus dilophonotae Cameron), Eulophidae (Chrysonotomyia sp. aff. serjaniae), Trichogrammatidae (Trichogramma marandobai Brun, Moraes & Soares) and Encyrtidae (Ooencyrtus sp.). The Erinnyis spp. eggs were mostly parasitized by T. dilophonotae, which was classified along with T. marandobai as constant. The occurrence of these natural enemies highlights the potential for natural biological control against Erinnyis spp.
Показать больше [+] Меньше [-]Ultrastructural studies of sensilla in one fly of forensic importance
2020
Cesar Carriço | Rebecca Leal Caetano | Thalyta Maria de Souza Nascimento | Zeneida Teixeira Pinto
Taxonomic identification is essential in the field of forensic entomology. Insects are important in the decomposition of cadavers. The flies are generally attracted to cadavers and one of the most important contributions is to estimate the postmortem interval. The scanning electron microscopy (SEM), which allows rapid and accurate identification of different species of forensics flies, has been highlighted as it allows better visualization of the external morphology of immature and some adults. The purpose of this study was to examine in details the morphological aspects of sensilla on the antennae of female and male of Xanthacrona bipustulata Wulp using SEM, since many of them could not be observed just by the use of light microscopy.
Показать больше [+] Меньше [-]Agricultural entomology in the state of Acre: a historical overview and future challenges
2020
Weidson Plauter Sutil | Rodrigo Souza Santos
In the state of Acre, Brazil, family-based agriculture is predominant and although it has less economic impact in relation to large-scale plantations, it is also subject to biological imbalances, such as the attack of pests, generating economic losses for producers. Phytosanitary knowledge in the state is still incipient and becomes an obstacle to production, often resulting in mistaken management decisions. In this context, this work aimed to present a history of research carried out in the field of Agricultural Entomology, especially in the areas of integrated management and biological control of pests of the most relevant crops grown in the Acre state. In this review, 55 scientific papers related to Agricultural Entomology were presented, 23 on new insect records and/or new insect associations with host plants, 16 on insect control using plant extracts from the Amazon region, 13 on integrated pest management and three on bioecology and population dynamics of pests in the state of Acre. Through this review, it is observed that research in the area of Agricultural Entomology in the state of Acre is characterized by basic research (surveys, registration of new species, bioecological and dynamic population studies). However, there has been a notable advance in scientific studies aimed at integrated pest management in the state in the past ten years. Still, there is a need to concentrate efforts on research aimed at prospecting and using Amazonian biodiversity (fauna and flora), which subsidize applied programs for integrated pest management of the main crops grown in the Acre state.
Показать больше [+] Меньше [-]Study of behavioral patterns and infection analyses in anopheline species involved in the transmission of malaria in Buriticupu and São José de Ribamar municipality, Maranhão State, Brazil
2020
Vera Lúcia Lopes de Barros | Fábio Medeiros da Costa | Antônio Rafael da Silva | Eloisa da Graça Rosário Gonçalves | Denilson da Silva Bezerra | Elias Seixas Lorosa | Wanderli Pedro Tadei
Anopheles darlingi Root and Anopheles aquasalis Curry are the main vectors of malaria that occur in the State of Maranhão. Entomological surveys based on the behavior and infectivity of these vectors are important for the elaboration of disease control strategies. The objectives of this work were to study the behavioral patterns of mosquitoes, determining population and hematophagic peaks, dietary preferences, infectivity rate and characterization of breeding sites in two municipalities in the State of Maranhão: Buriticupu and São José of Ribamar. Larvae and pupae were collected in breeding sites and adult females in home environments. Mosquito behavior, their dietary preferences and Plasmodium spp infection rates were analyzed. The vegetation and physicochemical patterns in the breeding sites found are in agreement with those described for species from the Amazon region and the Brazilian Atlantic coast. Anopheles darlingi was the most prevalent mosquito in Buriticupu breeding and home environments. This species was found mainly fed on human blood and naturally infected with Plasmodium vivax Grassi & Feletti and Plasmodium falciparum Welch. Anopheles aquasalis was more frequent in breeding sites in São José de Ribamar, as well as in home environments, whose specimens were mainly fed with human and bird blood. The main peaks of mosquito occurrence in Buriticupu were between 6 pm to 9 pm and in São José de Ribamar we did not record a definite peak. In the first municipality A. darlingi showed dominance over Anopheles albitarsis Lynch Arribálzaga s.l., Anopheles oswaldoi Peryassú, Anopheles nuneztovari Gabaldón and Anopheles evansae Brèthes,, besides presenting a correlation with rainfall. In the second municipality, A. aquasalis was dominant over A. albitarsis s.l. and there was a correlation between these two species and the rainy season. We conclude that the collected data contribute to elucidate the dynamics of malaria transmission in the region and guide the control actions directed to the elimination of the disease in the country.
Показать больше [+] Меньше [-]Nest camouflage records on five social wasp species (Vespidae, Polistinae) from southeastern Brazil
2020
Marcos Magalhães de Souza | Mateus Aparecido Clemente | Gabriel Teofilo-Guedes
Social insects use different strategies to defend their colonies, including camouflage. In order to expand knowledge, regarding the strategy of camouflage in social wasps, information is presented on the camouflage pattern of the species: Parachartergus smithii (De Saussure), Parachartergus wagneri Du Buysson, Chartergellus communis Richards, Metapolybia cingulata (Fabricius) and Mischocyttarus anthracinus Richards.These species are uncommon in inventory studies in Brazil. Records occur in different Conservation Units, between 2011 and 2019, in the Minas Gerais State, Brazil Southeastern. Camouflage seems to be the main defense strategy for these species, due to the color and shape of their nests in line with the substrate, as well as their docile behavior. The exception is Metapolybia cingulata, which in addition to camouflage shows aggressive behavior in some situations, which suggests the use of different strategies in the colony defense.
Показать больше [+] Меньше [-]Epicauta vittata (Fabricius): occurrence in tomato plant and weeds in the State of Rondônia
2020
Claudemir Schwanz Turcato | Freddy Melo Toledo | Emanoel Pereira Sampaio | Cleiton Dias Alves | Vando Miossi Rondelli | Jairo Rafael Machado Dias | Fábio Régis de Souza
The tomato (Solanum lycopersicum L.) plant stands out for being a species cultivated throughout the national territory, being the most important vegetable in Brazil, from the socioeconomic point of view. However, it has been one of the crops most affected by phytosanitary problems. For example, there are the pest insects of the genus Epicauta (Coleoptera: Meloidae). Thus, the objective of this study was to record the occurrence of a pest insect (beetle) harming vegetative growth and productive performance in the fresh-market tomato crop and feeding on weeds. The tomato plants were found on “jurubeba” (Solanum paniculatum L.) rootstock, spaced 1.0 m x 0.5 m, grown in the municipality of Rolim de Moura, Rondônia state. The insect-pest (beetle) was identified as Epicauta vittata (Fabricius). It was observed that the damages caused by the larvae occurred in the main stem of the tomato, only above the grafting site, causing boring, however no damage was observed in the rootstock (“jurubeba”). Adult insects caused defoliation in both tomato plant and joyweed (Alternanthera tenella Colla), slender amaranth (Amaranthus viridis L.), “trapoeraba” (Commelina benghalensis L.) and morning glory (Ipomoea purpurea [L.]). High population of E. vittata causes severe damages to tomato, damaging the vegetative growth and productive performance of the crop, however does not affect the root system when grafted with “jurubeba” (S. paniculatu). Epicauta vittata in the adult phase is fed alternately of weeds.
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