ABSTRACT Passiflora cincinnata Mast. has become more popular in the market because the unusual flavor of its fruits and natural beauty of its flowers, and has great potential for breeding programs of Passiflora edulis f. flavicarpa, because its resistance to diseases and drought. The objective of this work was to evaluate seven wild passion fruit (P. cincinnata) accessions, using morphological and agronomic descriptors and molecular markers type ISSR, to identify their morphoagronomic and genetic variabilities and potential for use in breeding programs. A randomized block experimental design was used with five replications and two plants per plot. Thirteen qualitative and twenty-one quantitative, vegetative and floral characteristics were used for morphoagronomic characterization. Twelve ISSR primers were evaluated for molecular characterization. Among the qualitative characteristics, only the color variations were significantly different between the accessions. According to the mean squares of the quantitative characteristics evaluated, obtained from analysis of variance, the means of accessions showed significant differences (p<0.01) for all characteristics. The IAL (internode average length) was the morphological descriptor that most contributed to diversity, with 43.12%, followed by DH5 (stem diameter at 5 cm height) and SW (sepal width). The average genetic similarity found was 68%. Despite the low genetic variability found among accessions, the primers UBC-887 and UBC-841 stood out with high percentage of polymorphism with 14 and 11 polymorphic fragments, respectively, and higher values of polymorphism information content (PIC), resolving power (RP) and marker index (MI), denoting suitability for use in diversity studies of P. cincinnata. Low variability was found among accessions evaluated. | RESUMO A espécie Passiflora cincinnata Mast. vem se popularizando no mercado pelo sabor incomum dos seus frutos, beleza natural de sua flores e possui grande potencial para a cultura de Passiflora edulis f. flavicarpa, pois apresenta resistência a doenças e déficit hídrico. Este trabalho teve como objetivo avaliar sete acessos de maracujá-do-mato (P. cincinnata) por meio de descritores morfológicos, descritores agronômicos e marcadores moleculares do tipo ISSR visando identificar variabilidade morfoagronômica e genética e o potencial para serem utilizados em programas de melhoramento. O delineamento experimental foi em blocos casualizados, com cinco repetições e duas plantas por parcela. Para caracterização morfoagronômica foram avaliadas 13 características qualitativas e 21 características quantitativas vegetativas e florais. Para caracterização molecular foram testados 12 primers de ISSR. Entre as características qualitativas apenas as variações de coloração apresentaram diferenças marcantes entre os diferentes acessos. De acordo com os quadrados médios obtidos das análises de variância para as características quantitativas avaliadas pode-se ressaltar as diferenças significativas (p<0,01) entre as médias dos acessos para todos os caracteres avaliados. Verificou-se que para os 21 descritores morfológicos avaliados, o que mais contribuiu para a diversidade foi o MI (média internódio) com 43,12%, seguido por DH5 (diâmetro das hastes a 5 centímetros do solo) e LS (largura da sépala). A similaridade genética média encontrada foi 68%. Apesar de ser diagnosticada baixa variabilidade genética entre os acessos avaliados, os primers UBC-887 e UBC-841 se destacaram com alto percentual de polimorfismo, com 14 e 11 fragmentos polimórficos respectivamente e valores altos para conteúdo da informação de polimorfismo (PIC), poder de resolução do primer (RP) e índice do marcador (MI) dos primers, demonstrando aptidão para serem utilizados em pesquisas de diversidade em P. cincinnata. Foi diagnosticada baixa variabilidade entre os acessos avaliados.

Passiflora cincinnata Mast. has become more popular in the market because the unusual flavor of its fruits and natural beauty of its flowers, and has great potential for breeding programs of Passiflora edulis f. flavicarpa, because its resistance to diseases and drought. The objective of this work was to evaluate seven wild passion fruit (P. cincinnata) accessions, using morphological and agronomic descriptors and molecular markers type ISSR, to identify their morphoagronomic and genetic variabilities and potential for use in breeding programs. A randomized block experimental design was used with five replications and two plants per plot. Thirteen qualitative and twenty-one quantitative, vegetative and floral characteristics were used for morphoagronomic characterization. Twelve ISSR primers were evaluated for molecular characterization. Among the qualitative characteristics, only the color variations were significantly different between the accessions. According to the mean squares of the quantitative characteristics evaluated, obtained from analysis of variance, the means of accessions showed significant differences (p<0.01) for all characteristics. The IAL (internode average length) was the morphological descriptor that most contributed to diversity, with 43.12%, followed by DH5 (stem diameter at 5 cm height) and SW (sepal width). The average genetic similarity found was 68%. Despite the low genetic variability found among accessions, the primers UBC-887 and UBC-841 stood out with high percentage of polymorphism with 14 and 11 polymorphic fragments, respectively, and higher values of polymorphism information content (PIC), resolving power (RP) and marker index (MI), denoting suitability for use in diversity studies of P. cincinnata. Low variability was found among accessions evaluated.