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SELEÇÃO ASSISTIDA POR MARCADORES DE DNA EM RETROCRUZAMENTO VISANDO RESISTÊNCIA AO MOFO BRANCO EM FEIJOEIRO
2012
RAFAELA PRISCILA ANTONIO | JOÃO BOSCO DOS SANTOS | FILIPE COUTO ALVES | PAULO ROBERTO CARVALHO GONÇALVES | LETÍCIA APARECIDA DE CASTRO LARA
The present study aimed to use DNA markers to select plants for the first and second backcross (BC) of two distinct populations of common bean, carrying the QTL for resistance to white mold and RAPD and microsatellite markers to identify the plants of the BC population more similar to their recurrent parent. For this, it was used the lines G122 (resistant - P1) as donor parent and VC3 (susceptible - P2) as recurrent parent for generation of the population F1RC1-GV evaluated for resistance to white mold by means of the SCAR Phs and genotyped with RAPD primers. In the F1RC2-EM population were used the lines Ex Rico 23 (resistant) as donor parent and M20 (susceptible) as recurrent parent evaluated for resistance to white mold, by means of RAPD primers O12.1600 and O15.1800 and genotyped with microsatellite (SSR) primers. Genetic similarity (sgij) between each BC plant and the recurrent parent was estimated using the Sorensen-Dice coefficient. The proportion of the SSR alleles derived from the recurrent parent was also estimated and, simultaneously, genetic similarity and the proportion of SSR alleles were efficient for identifying plants more similar to the recurrent parent. It was found that marker assisted selection (MAS) contributes to reducing the number of BC in at least a generation.
Показать больше [+] Меньше [-]SINOPSE DO GÊNERO Nymphaea L. (Nymphaeaceae) NO ESTADO DO CEARÁ
2012
DANILO JOSÉ LIMA DE SOUSA | LÍGIA QUEIROZ MATIAS
The family Nymphaeaceae is represented by aquatic rhizomatic herbs, rooted in the substrate, with floating long petiolate leaves. Its flowers are solitary, and anthesis can be nocturnal and diurnal, pollinated by beetles. The family has six genus, of which Nymphaea and Victoria occurs in the neotropical region. Victoria predominates in the Brazilian Amazon and Pantanal and the Nymphaea is represented by 18 species distributed throughout the country. The floristic study included the analysis of specimens collected on expeditions and materials deposited in Herbariums, in a total of 69 specimens. The identification was made by consulting specific bibliography. The information obtained enabled the preparation of an identification key, illustrations of important morphological characters, significant as evidence Taxonomy, commentaries on the geographical distribution and discussion of relevant issues to taxons identification. For the state of Ceará were found six species: N. amazonum, N. pulchella, N. Jamesoniana, N. lasiophylla, N. lingulata, N. rudgeana and N. tenerinervia. The last two populations presented only in coastal environments. N. lingulata, N. pulchella and N. Jamesoniana presented records in the arid region, in interior of the State. N. amazonum and N. lasiophylla have a wide distribution, being present in the coastal zone to the interior. N. lasiophylla was the most frequent species. Knowledge about the Flora of the State of Ceará contribute to future projects for the conservation and management of temporary and permanent aquatic environments that occur in the Brazilian semiarid.
Показать больше [+] Меньше [-]EMERGÊNCIA DE SEMENTES DE TIMBÓ EM DIFERENTES CONDIÇÕES DE LUZ
2012
MARIA DE FATIMA BARBOSA COELHO | VICTOR LOURENÇO SANCHES | RODRIGO ALEIXO BRITO DE AZEVEDO
The timbó (Magonia pubescens St. Hil.), tree typical of the cerrado, has medicinal, timber, craft, ornamental and larvicidal. The aim of this paper to evaluate seedling emergence of M. pubescens under different light conditions. The experiment was conducted in chambers B.O.D. temperature 30 °C, substrate sand, in a completely randomized design with five treatments and four replications of 50 seeds. The treatments were: 1) a photoperiod of 24 hours, 2) absence of light, 3) a photoperiod of 12 hours, 4) a photoperiod of 8 hours and 5) a photoperiod of 16 hours. The smallest percentage and emergence rate (45% and 14 days) were provided 12 h light and 12 h dark, and the emergence in all treatments was high (90 to 97.5%) and emergency speed too (7.4 to 10.5 days). The seeds of M. pubescens photoblastic are neutral in laboratory conditions at a temperature of 30 ºC and the emergence occurs between four and nine days.
Показать больше [+] Меньше [-]CRESCIMENTO E DESEMPENHO DE BERTALHA (Basella alba L.) EM FUNÇÃO DO TIPO DE PROPAGAÇÃO
2012
RENÊ ARNOUX DA SILVA CAMPOS | GIUSEPPINA PACE PEREIRA LIMA | SANTINO SEABRA JÚNIOR | WILLIAM HIROSHI SUEKANE TAKATA | EWERTON GASPARETTO DA SILVA
Malabar spinach is a non-conventional vegetable propagated mainly by seeds. This research evaluated the propagation by stem cuttings as an alternative method of propagation and verified its effect on growth of the vegetable. Plantlets propagated by seeds and stem cuttings were grown in greenhouse and their growth were evaluated in five successive intervals of 21 days each, with the first evaluation carried out 60 days after the transplanting. The results showed that the propagation methods have influence on the Malabar spinach growth. Plants propagated by seeds display slow, but vigorous growth, with larger relative yield of leaves and tender leaves, while plants propagated by cuttings showed fast initial growth, with rapid fruit formation. Thus, the propagation by cuttings is more suitable for seed production, while propagation by seeds is more profitable for leaves production.
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