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EARLY DEVELOPMENT OF CASTOR BEANS GROWN UNDER SALINITY CONDITIONS (VARIETIES BRS ENERGIA, MPA 34 AND MPB 01) Полный текст
2016
PRESOTTO, RAFAEL ANTÔNIO | ALVES, GHILHERME ZOLLI | NASCIMENTO, ELISAMARA CALDEIRA DO | GENUNCIO, GLAUCIO DA CRUZ | ZONTA, EVERALDO
ABSTRACT Studies have shown that some plant species exhibit satisfactory production levels when grown under high salinity levels, whereas others exhibit decreased production due to sodium sensitivity even at low sodium concentrations. The castor bean is moderately sensitive to salinity. The aim of the present study was to evaluate the early growth of three castor bean varieties (BRS Energia, MPA 34 and MPB 01) grown in nutrient solution with increasing sodium concentrations (control, 25, 50, 75 and 100 mmol L-1). A completely randomized split-plot experimental design was used, with a 5x3 factorial scheme and three replicates per treatment. Salinity resulted in decreased dry weight of all castor bean varieties grown for 31 days under hydroponic conditions. The dry weight accumulation was less affected at the root than at the shoot level. Nevertheless, the shoot dry weight decreased with the increasing salinity. MPA 34 exhibited higher early growth than the remaining tested varieties. Salinity affected the early development of the tested castor bean varieties, and this effect was more pronounced at the shoot than at the root. Variety MPA 34 is promising for cultivation under moderate salinity levels. | RESUMO Pesquisas demonstram produções satisfatórias de espécies quando cultivadas em altos níveis de salinidade, enquanto outras reduzem a sua produção por apresentarem sensibilidade ao sódio, mesmo em baixas concentrações. A mamoneira é moderadamente sensível à salinidade e, neste sentido, este trabalho teve por objetivo avaliar o crescimento inicial de três cultivares de mamoneira (BRS Energia, MPA34 e MPB 01), cultivadas em solução nutritiva, com concentrações crescentes de sódio (controle; 25; 50; 75 e 100 mmol L -1). O delineamento experimental foi inteiramente casualizado, em fatorial 5x3 e parcela subdividida, com três repetições. A salinidade reduziu a matéria seca de todas as variedades de mamoneira cultivadas por 31 dias em sistema hidropônico. Entretanto, observou-se que o sistema radicular foi menos afetado quanto ao acúmulo de matéria seca, quando comparado ao acúmulo da parte aérea. Ainda assim, a matéria seca da parte aérea reduziu gradativamente a partir do aumento da salinidade. A variedade MPA 34 apresentou maior crescimento inicial, quando comparada as demais variedades. A salinidade afetou o desenvolvimento inicial das variedades de mamoneira, sendo a parte aérea mais afetada que o sistema radicular. O cultivo da variedade MPA 34 demonstrou-se promissor sob condições de moderados níveis de salinidade.
Показать больше [+] Меньше [-]SAMPLE SIZE FOR ASSESS THE LEAF BLAST SEVERITY IN EXPERIMENTS WITH IRRIGATED RICE Полный текст
2016
SARI, BRUNO GIACOMINI | LÚCIO, ALESSANDRO DAL'COL | COSTA, IVAN FRANCISCO DRESSLER DA | RIBEIRO, ANA LÚCIA DE PAULA
RESUMO O objetivo deste trabalho foi determinar o tamanho de amostra necessário para avaliar a severidade da brusone da folha no arroz irrigado em experimentos com diferentes tratamentos fungicidas. Foram utilizados dados de severidade da brusone e área abaixo da curva de progresso da doença de três experimentos utilizando o controle químico realizados no Rio Grande do Sul. Foi realizada a análise de variância, para verificar se a severidade da doença foi diferenciada entre os tratamentos. Também foi verificado se a dispersão da doença foi diferenciada entre os tratamentos e as avaliações, através da razão variância/média e do índice de Morisita. A dispersão da doença entre os tratamentos e ao longo das avaliações é importante para a escolha da fórmula utilizada no cálculo do tamanho da amostra. A dispersão da doença não foi a mesma ao longo dos experimentos, variando entre tratamentos e avaliações. Diante deste comportamento, optou-se por utilizar uma fórmula de cálculo que não estivesse associado a distribuições que indicassem a distribuição espacial da doença no campo (binomial negativa ou Poisson). O tamanho de amostra para a estimação da severidade média da brusone do arroz variou entre os tratamentos e as avaliações. Para avaliar a área abaixo da curva de progresso da doença é necessário avaliar menos folhas. Recomenda-se a avaliação de 293 folhas para estimar a severidade, e 63 para estimar a AUDPC, com 20% de erro. | ABSTRACT The aim of this study was to determine the sample size needed to assess the severity of leaf blast in rice in experiments with different fungicide treatments. The severity and the area under the disease progress curve data of three chemical disease control treatments carried out in Rio Grande do Sul, were used in the study. Analysis of variance was performed to verify whether the severity of the disease differed between treatments. The spread of disease was was also found to be different between treatments and assessments, using the variance/mean ratio and Morisita index. The spatial distribution of the disease among the treatments and during the evaluations is important for the choice of the equation used to calculate the sample size. The spatial distribution of the disease was not the same across the experiments, and it varied between treatments and evaluations. Thus, we decided to use a formula that was not associated with distributions to indicate the spatial distribution (negative binomial or Poisson) of the disease in the field. The sample size to estimate the average of rice leaf blast severity varied between treatments and evaluations. The area under the disease progress curve is necessary to be determined to reduce the number of samples needed. Thus, it is recommended to assess 293 sheets to estimate severity, and 63 to estimate AUDPC at 20% error.
Показать больше [+] Меньше [-]EFFECTS OF PLANTING DENSITYAND ORGANIC FERTILIZATION DOSES ON PRODUCTIVE EFFICIENCY OF CACTUS PEAR Полный текст
2016
SILVA, NALÍGIA GOMES DE MIRANDA E | SANTOS, MÉRCIA VIRGINIA FERREIRA DOS | DUBEUX JÚNIOR, JOSÉ CARLOS BATISTA | CUNHA, MÁRCIO VIEIRA DA | LIRA, MÁRIO DE ANDRADE | FERRAZ, IVAN
RESUMO A palma tem grande importância para a pecuária da região semiárida do Brasil. Essa planta vem apresentando elevada produtividade de forragem, influenciada por vários fatores de manejo. Objetivou-se avaliar o efeito de doses de adubação orgânica (20, 40 e 80 t/ha de esterco bovino/ha/dois anos) e densidades de plantio (20, 40, 80 e 160 mil plantas/ha) sobre a produtividade da palma forrageira Clone IPA-20 (Opuntia ficus indica Mill). O experimento foi conduzido na Estação Experimental de Caruaru, pertencente ao Instituto Agronômico de Pernambuco - IPA. O delineamento experimental foi em blocos casualizados, com arranjo de parcelas subdivididas. Verificou-se maior produtividade de parte aérea com o aumento da densidade populacional e aplicação de 80 t de esterco/ha/dois anos, com valores de 61; 90; 117 e 139 t MS/ha/dois anos, nas densidades de 20, 40, 80 e 160.000 plantas/ha. A produtividade do cladódio-planta e peso seco da raiz foram influenciados pela densidade de plantio, apresentando respostas exponenciais, tendendo a apresentar maior peso do cladódio planta e raízes por área com a elevação da densidade de plantio. A eficiência da adubação orgânica diminuiu com a elevação das doses de esterco. Recomenda-se para plantios com 160.000 plantas/ha aplicação de, no mínimo, 40 t de esterco bovino/ha/dois anos para incremento na produtividade da palma. | ABSTRACT Cactus is crucial for the livestock of semi-arid regions in Brazil. This plant has shown the high productivity of forage, which is influenced by several management factors. This study aimed to evaluate the effect of organic fertilization doses (20, 40 and 80 t/ ha of bovine manure/ha/two years) and planting densities (20, 40, 80 and 160 thousand plants/ha) on the productivity of cactus pear Clone IPA-20 (Opuntia ficus-indica Mill). At the Experimental Station of Caruaru at the Agronomic Institute of Pernambuco, IPA has conducted the experiment. The experimental design was randomized blocks, with split plot arrangements. Higher shoot productivity was observed with increased population density and the application of manure at 80 t ha-1 two years-1 with values of 61, 90, 117 and 139 t DM ha-1 two years-1 at densities of 20, 40, 80 and 160,000 plants ha-1. The planting density influenced the productivity of cladode-plant and root dry weight, showing exponential responses, with higher cladode-plant and roots weight by area observed with increased plant density. The efficiency of organic fertilization decreased with the increase in manure doses. For increase cactus productivity, 40 t of bovine manure ha-1 two years-1 for plantations with 160,000 plants/ha is recommended.
Показать больше [+] Меньше [-]GROWTH AND MICRONUTRIENT CONCENTRATION IN MAIZE PLANTS UNDER NICKEL AND LIME APPLICATIONS Полный текст
2016
GILMAR NUNES TORRES | SÂNIA LÚCIA CAMARGOS | OSCARLINA LUCIA DOS SANTOS WEBER | KELLY DAYANA BENEDET MAAS | WALCYLENE MATOS PEREIRA SCARAMUZZA
The experiment was conducted in a greenhouse located at the Federal University of Mato Grosso, Cuiaba - MT, from March to May 2012. The objective was to assess the effects of different rates of nickel application with and without liming on maize growth and micronutrient levels. The study was a randomized block design in a 2 x 5 factorial arrangement with four replicates, for a total of 40 plots, including with and without liming and five rates of nickel application, on a clayey Red Yellow Latosol (Oxisol, USDA classification and Ferralsol, FAO classification). Both lime and nickel applications influenced plant growth, reducing plant development with increased nickel application without liming. It was also observed that both lime and nickel applications altered micronutrient levels in the maize plants, independent of which part of the plant was evaluated. Nickel played an antagonistic role with manganese and zinc and a synergistic role with copper and iron.
Показать больше [+] Меньше [-]MODELING OF FUEL CONSUMPTION FOR FOREST TRANSPORTATION Полный текст
2016
POMPEU PAES GUIMARÃES | JULIO EDUARDO ARCE | EDUARDO DA SILVA LOPES | ALLAN LIBANIO PELISSARI | GABRIELA SALAMI | VINICIUS GOMES DE CASTRO
MODELING OF FUEL CONSUMPTION FOR FOREST TRANSPORTATION Полный текст
2016
POMPEU PAES GUIMARÃES | JULIO EDUARDO ARCE | EDUARDO DA SILVA LOPES | ALLAN LIBANIO PELISSARI | GABRIELA SALAMI | VINICIUS GOMES DE CASTRO
As fuel costs increase, it is essential to take measures involving planning and control on any activities with high consumption. Thus, the main aim of this work was modeling the fuel consumption of forest road transportation by truck. We collected data about time, driving distance, average speed, fuel consumption and the load carried by the vehicle for loaded trips, unloaded trips, and the total cycle of forest transport in regions between the municipality of Campo do Tenente (forest site) and Piên (factory) located in Paraná state, Brazil. The Pearson’s correlation was used to determine the relationship between variables, while the Stepwise procedure was used to generate regression equations to estimate fuel consumption. The highest correlations were found between fuel consumption and driving distance, average speed and liquid weight of the load; also, there was a significant correlation between driving distance and average speed. Adjusted equations were statistically adequate to estimate fuel consumption based on driving distance, liquid weight of the load, average speed and duration time for loaded trips, unloaded trips and the total forest road transportation cycle.
Показать больше [+] Меньше [-]MODELING OF FUEL CONSUMPTION FOR FOREST TRANSPORTATION Полный текст
2016
GUIMARÃES, POMPEU PAES | ARCE, JULIO EDUARDO | LOPES, EDUARDO DA SILVA | PELISSARI, ALLAN LIBANIO | SALAMI, GABRIELA | CASTRO, VINICIUS GOMES DE
RESUMO: Com o aumento nos custos dos combustíveis, é fundamental atuar em medidas que envolvam o planejamento e o controle nas atividades com consumo elevado. Dessa forma, objetivou-se modelar o consumo de combustível da carreta utilizada no transporte rodoviário florestal. Para isso, dados de duração, distância percorrida, velocidade média, consumo de combustível e carga transportada pelos veículos nas operações das viagens carregado, vazio e ciclo total foram coletados nas regiões de Campo do Tenente (planta florestal) a Piên (planta industrial) no estado do Paraná. A correlação linear de Pearson foi utilizada para determinar a relação entre as variáveis, ao passo que o procedimento Stepwise foi empregado para compor as equações de regressão na estimativa do consumo de combustível. As maiores correlações foram observadas entre a distância percorrida, carga líquida e velocidade média com o consumo de combustível; ocorrendo, também, correlações significativas entre a distância percorrida e a velocidade média. As equações ajustadas foram estatisticamente adequadas para estimar o consumo de combustível em função da distância percorrida, da carga líquida transportada, da velocidade média e da duração nas viagens carregado, vazio e ciclo total de transporte rodoviário florestal. | ABSTRACT: As fuel costs increase, it is essential to take measures involving planning and control on any activities with high consumption. Thus, the main aim of this work was modeling the fuel consumption of forest road transportation by truck. We collected data about time, driving distance, average speed, fuel consumption and the load carried by the vehicle for loaded trips, unloaded trips, and the total cycle of forest transport in regions between the municipality of Campo do Tenente (forest site) and Piên (factory) located in Paraná state, Brazil. The Pearson's correlation was used to determine the relationship between variables, while the Stepwise procedure was used to generate regression equations to estimate fuel consumption. The highest correlations were found between fuel consumption and driving distance, average speed and liquid weight of the load; also, there was a significant correlation between driving distance and average speed. Adjusted equations were statistically adequate to estimate fuel consumption based on driving distance, liquid weight of the load, average speed and duration time for loaded trips, unloaded trips and the total forest road transportation cycle.
Показать больше [+] Меньше [-]PERFORMANCE AND QUANTITATIVE AND QUALITATIVE CARCASS TRAITS IN PIGS FED ON CANOLA MEAL Полный текст
2016
LINA MARÍA PEÑUELA SIERRA | IVAN MOREIRA | PAULO LEVI DE OLIVEIRA CARVALHO | ADRIANA GOMEZ GALLEGO | LAURA MARCELA DIAZ HUEPA | CLODOALDO DE LIMA COSTA FILHO
PERFORMANCE AND QUANTITATIVE AND QUALITATIVE CARCASS TRAITS IN PIGS FED ON CANOLA MEAL Полный текст
2016
LINA MARÍA PEÑUELA SIERRA | IVAN MOREIRA | PAULO LEVI DE OLIVEIRA CARVALHO | ADRIANA GOMEZ GALLEGO | LAURA MARCELA DIAZ HUEPA | CLODOALDO DE LIMA COSTA FILHO
Two experiments were performed for the purpose of determining the nutritional value and assess the performance and carcass traits in pigs fed on canola meal (Brassica napus). Experiment I consisted of a total digestibility trial conducted with 14 castrated commercial crossbred male pigs with 60.69 ± 4.26 kg initial live weight. The treatments consisted of a control diet based on maize and soybean meal and another diet containing canola meal (control + 25% canola meal). Each pig was an experimental unit, totalling seven experimental units per diet. The digestible energy and the metabolizable energy of canola meal used was 2,999 and 2,730 kcal.kg-1, respectively. These values were used to formulate the diets of the performance experiment. Experiment II included 50 crossbred pigs with an average initial live weight of 29.90 ± 1.16 kg and an average final live weight of 60.33 ± 3.38 kg during the growing phase, and an average initial live weight of 60.37 ± 1.6 kg and an average final live weight of 90.37 ± 3.19 kg during the finishing phase. The experimental design consisted of randomized blocks, with four treatments (6, 12, 18, and 24% canola meal), 10 repetitions, and one pig per experimental unit. The control diet was formulated with 0% canola meal. The data were assessed using ANOVA test and the averages of the treatments were compared using Dunnett's test and regression analysis at a 5% probability level. The results suggest that it is possible to use up to 24% canola meal in the diets for pigs during the growing and finishing phases without affecting the performance and carcass traits.
Показать больше [+] Меньше [-]PERFORMANCE AND QUANTITATIVE AND QUALITATIVE CARCASS TRAITS IN PIGS FED ON CANOLA MEAL Полный текст
2016
PEÑUELA SIERRA, LINA MARÍA | MOREIRA, IVAN | CARVALHO, PAULO LEVI DE OLIVEIRA | GALLEGO, ADRIANA GOMEZ | DIAZ HUEPA, LAURA MARCELA | COSTA FILHO, CLODOALDO DE LIMA
RESUMO Foram conduzidos dois experimentos com o objetivo de determinar o valor nutricional e avaliar o desempenho e características da carcaça de suínos alimentados com farelo de canola (Brassica napus). No Experimento I, foi conduzido um ensaio de digestibilidade total com 14 suínos mestiços de linhagem comercial, machos castrados, com 60,69 ± 4,26 kg de PV inicial. O alimento teste farelo de canola (FC), substituiu em 25% a ração referência à base de milho e farelo de soja. Cada suíno constituiu uma unidade experimental, totalizando sete unidades experimentais por ração. Os valores de energia digestível (ED) e energia metabolizável (EM) na matéria natural para o FC foram de 2995 kcal/kg e 2796 kcal/kg, respectivamente. No Experimento II, foram utilizados 50 suínos mestiços, com peso vivo inicial de 29,90 ± 1,16 kg e final de 60,33 ± 3,38 kg, na fase de crescimento, e peso vivo inicial de 60,37 ± 1,6 kg e final de 90,37 ± 3,19 kg na fase de terminação. Foi utilizado o delineamento de blocos inteiramente casualizados, com quatro tratamentos, (6, 12, 18 e 24% de FC), com 10 repetições e um suíno por unidade experimental. Adicionalmente, foi formulada uma ração testemunha, contendo 0% de FC. Os dados foram submetidos à análise de variância e adicionalmente foi aplicado o Teste de Dunnett, e análise de regressão, a 5% de probabilidade. Os resultados sugerem que é possível incluir até 24% de FC nas dietas de suínos, em crescimento e terminação, sem prejudicar o desempenho e as características da carcaça. | ABSTRACT Two experiments were performed for the purpose of determining the nutritional value and assess the performance and carcass traits in pigs fed on canola meal (Brassica napus). Experiment I consisted of a total digestibility trial conducted with 14 castrated commercial crossbred male pigs with 60.69 ± 4.26 kg initial live weight. The treatments consisted of a control diet based on maize and soybean meal and another diet containing canola meal (control + 25% canola meal). Each pig was an experimental unit, totalling seven experimental units per diet. The digestible energy and the metabolizable energy of canola meal used was 2,999 and 2,730 kcal.kg-1, respectively. These values were used to formulate the diets of the performance experiment. Experiment II included 50 crossbred pigs with an average initial live weight of 29.90 ± 1.16 kg and an average final live weight of 60.33 ± 3.38 kg during the growing phase, and an average initial live weight of 60.37 ± 1.6 kg and an average final live weight of 90.37 ± 3.19 kg during the finishing phase. The experimental design consisted of randomized blocks, with four treatments (6, 12, 18, and 24% canola meal), 10 repetitions, and one pig per experimental unit. The control diet was formulated with 0% canola meal. The data were assessed using ANOVA test and the averages of the treatments were compared using Dunnett's test and regression analysis at a 5% probability level. The results suggest that it is possible to use up to 24% canola meal in the diets for pigs during the growing and finishing phases without affecting the performance and carcass traits.
Показать больше [+] Меньше [-]DIVERSITY AND GENETIC STRUCTURE OF NATURAL POPULATIONS OF ARAÇÁ (Psidium guineense Sw.) Полный текст
2016
EDSON FERREIRA DA SILVA | RAFAELA LIMA DE ARAÚJO | CRISTINA DOS SANTOS RIBEIRO MARTINS | LUIZA SUELY SEMEN MARTINS | ELIZABETH ANN VEASEY
Psidium guineense Sw , popularly known as araçá, is a fruit tree there is widely distributed in Brazil and belongs to the Myrtaceae family. In northeastern Brazil, araçá occurs along coast and in the Zona da Mata; its fruit looks like guava but is more acidic and has a stronger smell. There is a little information about this species, which increases the difficulty of conserving its genetic resources and exploiting araçá as an economic resource. The objective of this research was the evaluation of the genetic diversity and genetic structure of P. guineense from Pernambuco’s Zona da Mata. One hundred and fourteen individuals and 18 isozyme loci were evaluated, showing 28 alleles. The percentage of polymorphic loci ( ) and the average number of alleles per locus ( ) were 0.5 and 1.5, respectively, in this population. The expected heterozygosity , which corresponds to the genetic diversity, ranged from 0.22 to 0.23, a high value when considering that isozymes mark access from the functional genome. The differentiation index among the population was ( ) = 0.015; therefore, the populations were not different among the sampled places. The inbreeding values ( ) ranged from - 0.549 to - 0.794, indicating an absence of inbreeding and a greater - than - expected heterozygosity in all the studied populations. The estimated gene flow ( N m ) for a pair of this population ranged from 3.23 to 20.77, sufficient to avoid genetic differentiation among the population and in accordance with the values of genetic divergence found in this study.
Показать больше [+] Меньше [-]QUALITY AND ANTIOXIDANT ACTIVIY OF TOMATO CULTIVATED UNDER DIFFERENT SOURCES AND DOSES OF NITROGEN Полный текст
2016
JOHN SILVA PORTO | TIYOKO NAIR HOJO REBOUÇAS | MARIA OLIMPIA BATISTA MORAES | MARINÊS PEREIRA BOMFIM | ODAIR LACERDA LEMOS | JOSÉ MAGNO QUEIROZ LUZ
Tomatoes are an important component of the human diet because they are rich in minerals and antioxidants that sequester free radicals in cells, preventing various disorders that affect human health. Here, we aimed to evaluate the effects of different nitrogen sources and concentrations on antioxidant capacity and physical and chemical quality of tomato fruit. The experiment was conducted in Vitória da Conquista, state of Bahia, Brazil, in the experimental field of Universidade Estadual do Sudoeste da Bahia. The experimental setup included a randomized block design with four replicates in a factorial layout of 3 × 3 + 1 (control), for a total of 40 plots. The three treatments comprised different nitrogen sources (calcium nitrate, urea, and ammonium sulfate), applied in three doses (140, 280, and 420 kg ha - 1 ). We evaluated firmness, pH, titratable acidity - TA, soluble solid - SS, SS/TA ratio, ascorbic acid, carotenoids, flavonoids, phenolic compounds and DPPH inhibition, were evaluated after samples were harvested. However, increasing concentrations of N adversely affected the acidity and led to an increase in the SS/TA ratio. Increasing the N concentration also reduced the content of bioactive compounds. excluding carotenoids, which consequently impaired antioxidant activity.
Показать больше [+] Меньше [-]GROWTH, PHYSIOLOGY AND YIELD OF FORMOSA ‘PAPAYA’ CULTIVATED UNDER DIFFERENT DOSES OF COATED AND CONVENTIONAL UREA Полный текст
2016
GABRIEL BARBOSA DA SILVA JÚNIOR | ÍTALO HERBERT LUCENA CAVALCANTE | EDUARDO MONTEIRO SANTOS | FRANCISCA GISLENE ALBANO | ADRIEL MOURA SILVA
Mineral fertilizer management is one of the most important agronomic techniques applied in papaya cultivation, which generally extracts large amounts of nutrients from soil, especially nitrogen. This study aimed to assess the phytotechnical and physiological attributes and yield of „Formosa‟ papaya hybrid Caliman 01 as a function of different doses of coated and conventional urea in the region of Bom Jesus - PI, Brazil. The treatments were arranged in a factorial scheme (2 × 4) and distributed in a randomized block design with four replications, corresponding to 2 nitrogen sources (coated urea, 43% of N; and conventional urea, 45% of N) and 4 doses nitrogen (350, 440, 530 and 620 g per plant). The N doses used corresponded to 80, 100, 120 and 140% of the nitrogen fertilization. It was used six plants per plot with two row borders, totaling 192 plants cultivated in the field. It was assessed the intercepted photosynthetically active radiation (int.PAR), radiation interception efficiency (PAREff.), leaf area index (LAI), plant height, stem diameter and yield. The coated urea promotes a higher growth and yield of „Formosa‟ papaya compared to the conventional urea. The application of 1.0 g of coated N promotes the production of 2.87 g of fruit per hectare while the application of 1.0 g of conventional N produces only 1.89 g of fruit per hectare, showing that the source of polymerized N increases the use efficiency of N applied to soil.
Показать больше [+] Меньше [-]INHIBITION OF PATHOGENS BY SPOROGENIC BACTERIA ISOLATED FROM HONEY OF Melipona sp. (APIDAE: APINAE: MELIPONINI) Полный текст
2016
KELY DAMIANA NOVAES DA SILVA | RENAN DO NASCIMENTO BARBOSA | PEDRO DE ASSIS DE OLIVEIRA | MARCELO CASIMIRO CAVALCANTE | HÉLIO FERNANDES DE MELO
The aim of this study was to isolate sporogenic bacteria from the honey of stingless bees Melipona sp., in dry forest, and to evaluate their antagonistic potential for medicinal employment purposes and animal production. The honey samples were collected in Serra Talhada - PE, where honey was taken from four different hives (in triplicate), totaling 12 samples. The samples were diluted and subjected to 80 ºC for 20 minutes to eliminate vegetative cells. The dilutions were plated onto nutrient agar and incubated at 30 ºC for 72 hours. Then the colony forming units (CFU) were quantified. The samples were also plated onto malt agar and Sabouraud agar, and incubated at 30 ºC for 14 days for the growth of yeast and molds. Total and fecal coliforms were quantified by the most probable number method (MPN). Seven isolates (I) of sporogenic bacteria ( Bacillus ) were obtained, however only four showed probiotic potential. Isolate I - 5 showed the greatest probiotic potential and inhibited the growth of Escherichia coli , Klebsiella sp., Pseudomonas aeruginosa, Salmonella sp., and Staphylococcus aureus . The growth of the Sarcina sp. was not inhibited by any isolate. No yeast, molds or coliforms were found. The Melipona sp. honey is a source of spore - forming bacteria and is antagonistic to microorganisms that contaminate honey. It has good microbiological quality.
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