The aim of this study was to apply optical microscopy and scanning electron microscopy (SEM) techniques to identify and assess dripper clogging of an irrigation system. Three dripper models (Taldrip, Streamline, and Tiran) underwent 1200 hours of irrigation with three types of effluents: treated domestic wastewater, water with a high calcium content, and urban water supply. Samples of the material adhered to the inside of drippers were analyzed by means of the smear method aiming at their biological identification. Other samples were taken to obtain SEM images. The presence of algae, bacteria, and biological components of biofilm was observed in drippers operating with wastewater and water with calcium, this latter in a low number. The formation of biofilm and chemical precipitates of calcium were observed in SEM images, leading to emitter clogging. Optical microscopy and SEM analyses allowed identifying the degree of fouling by biofilm and chemical precipitates in dripper clogging.

ABSTRACT The aim of this study was to apply optical microscopy and scanning electron microscopy (SEM) techniques to identify and assess dripper clogging of an irrigation system. Three dripper models (Taldrip, Streamline, and Tiran) underwent 1200 hours of irrigation with three types of effluents: treated domestic wastewater, water with a high calcium content, and urban water supply. Samples of the material adhered to the inside of drippers were analyzed by means of the smear method aiming at their biological identification. Other samples were taken to obtain SEM images. The presence of algae, bacteria, and biological components of biofilm was observed in drippers operating with wastewater and water with calcium, this latter in a low number. The formation of biofilm and chemical precipitates of calcium were observed in SEM images, leading to emitter clogging. Optical microscopy and SEM analyses allowed identifying the degree of fouling by biofilm and chemical precipitates in dripper clogging. | RESUMO O objetivo da pesquisa foi aplicar as técnicas de microscopia óptica e Microscopia Eletrônica de Varredura (MEV) para identificar e avaliar os causadores da obstrução dos gotejadores de irrigação localizada. Três modelos de gotejadores (Taldrip, Streamline e Tiran) foram submetidos a 1200 horas de irrigação com três tipos de efluentes: água residuária de esgoto doméstico tratado água com elevado teor de cálcio e água de abastecimento urbano. Amostras do material aderida no interior dos gotejadores foram analisadas pelo método de esfregaço para fazer a identificação biológica, outras amostras foram retiradas para realizar as imagens com MEV. Foi possível verificar a presença de algas, bactérias e componentes biológicos do biofilme, tanto nos gotejadores operando com água residuária, quanto naqueles que operavam com água com cálcio, esta última em menor quantidade. Nas imagens com MEV foi possível verificar a formação de biofilme que causou a obstrução dos emissores e também os precipitados químicos de cálcio. A análise por MEV e microscopia óptica permitiu identificar o grau de incrustação por biofilme e precipitados químicos na obstrução de gotejadores.

The northeastern semiarid region faces some of the droughts of the last centuries, resulting in serious reductions of agriculture yields. The water scarcity for agricultural requires rational use practices and reuse of water, such as use of domestic effluent sewage. Alternatively, the use of treated domestic sewage effluent utilizing for irrigation of forage species, such as the forage cactus, can be highlighted. In order to evaluate the effects of fertirrigation with treated sewage effluent on mineral nutrient composition of forage cactus (Opuntia Tuna L. Mill, Mexican elephant ear), an experiment was carried out utilizing a split plot 3 x (4 + 1) with a randomized complete block design randomized block and four replications, being three planting density (66,667.00, 52,282.00 and 33,333.00 plants ha-1) and two water sources associated with irrigation frequency of plates fixed on 3.5 mm per irrigation (Frequencies of 2.3 days with water tap and 7, 14 and 21 days with Sewage Effluent) and cactus cultivated in Rainfed agriculturerainfed (control). The analyze of the nutritional contents of cladodes indicated that there was a significant effect of the intervals from irrigation events only for Ca and Mg concentrations. The highest levels of Ca and Mg were found with the irrigation frequency of 2.3 using tap water. The macronutrients concentration in greater quantity by the forage cactus were N e, K followed by, Ca, Mg and P. The nutritional balance indicated excess of K and P and deficiency of N, Ca and Mg, in this order.

RESUMO O semiárido nordestino enfrenta umas das mais severas secas dos últimos séculos, resultando em reduções severas na produção agrícola. A escassez hídrica na agricultura exige práticas de uso racional e reaproveitamento da água como, por exemplo, a utilização de efluentes de esgoto doméstico . Alternativamente pode-se destacar o uso de esgoto doméstico tratado para irrigação de espécies forrageiras, como a palma. Objetivando avaliar os efeitos da fertirrigação com efluente de esgoto doméstico tratado sob a composição mineral de nutriente em palma forrageira (Opuntia Tuna L. Mill, Orelha-de-elefante Mexicana), foi conduzido um experimento em blocos casualizados em esquema de parcelas subdivididas 3 x (4 + 1), com 4 repetições, sendo três densidades de plantio (66.667,00; 52.282,00 e 33.333,00 plantas ha-1) e duas fontes hídrica associadas a frequência de irrigação com lâmina fixa de 3,5 mm por rega (Frequências de 2,3 dias com água de abastecimento e de 7, 14 e 21 dias com Efluente de Esgoto) e, palma cultivada em sequeiro (testemunha). As análises dos teores nutricionais dos cladódios indicam que houve efeito significativo dos intervalos entre eventos de irrigação apenas para as concentrações de Ca e Mg. Os maiores teores de Ca e Mg foram encontrados com a frequência de irrigação de 2,3 utilizando água de abastecimento. Os macronutrientes em maior quantidade pela palma forrageira foram N e K seguido de Ca, Mg e P. O balanço nutricional indicou excesso de K e P e déficit de N, Ca e Mg, nesta ordem. | ABSTRACT The northeastern semiarid region faces some of the droughts of the last centuries, resulting in serious reductions of agriculture yields. The water scarcity for agricultural requires rational use practices and reuse of water, such as use of domestic effluent sewage. Alternatively, the use of treated domestic sewage effluent utilizing for irrigation of forage species, such as the forage cactus, can be highlighted. In order to evaluate the effects of fertirrigation with treated sewage effluent on mineral nutrient composition of forage cactus (Opuntia Tuna L. Mill, Mexican elephant ear), an experiment was carried out utilizing a split plot 3 x (4 + 1) with a randomized complete block design randomized block and four replications, being three planting density (66,667.00, 52,282.00 and 33,333.00 plants ha-1) and two water sources associated with irrigation frequency of plates fixed on 3.5 mm per irrigation (Frequencies of 2.3 days with water tap and 7, 14 and 21 days with Sewage Effluent) and cactus cultivated in Rainfed agriculturerainfed (control). The analyze of the nutritional contents of cladodes indicated that there was a significant effect of the intervals from irrigation events only for Ca and Mg concentrations. The highest levels of Ca and Mg were found with the irrigation frequency of 2.3 using tap water. The macronutrients concentration in greater quantity by the forage cactus were N e, K followed by, Ca, Mg and P. The nutritional balance indicated excess of K and P and deficiency of N, Ca and Mg, in this order.

Plant growth promoter microorganisms have been studied as important tools for increasing crop production. Lettuce is the most consumed hardwood crop in the world. Numerous microorganisms are capable of acting in a beneficial way in the growth of this culture. The objective of the present study was to evaluate the efficacy of Trichoderma and Pseudomonas on the microbial biomass, enzymatic activities in sandy soil and lettuce production. The experimental design was completely randomized with ten replicates and treatments: CONT (absolute control); CM (control with cattle manure fertilization); CMB (with fertilization and Pseudomonas sp.); CMF (with fertilization and T. aureoviride) and CMBF (with fertilization and the two microorganisms combined). The fertilizer used was organic with cattle manure in a dose recommended for the culture. This study evaluated the production of lettuce, microbial biomass and the enzymatic activity of acid phosphatase, alkaline phosphatase and urease. The combined application of CMBF was efficient in increasing lettuce production, because it increased 85% of the cv. Veronica cultivated on sandy soil. The combined use of plant growth promoting microorganisms resulted to an increase in microbial biomass. In lettuce crops, it is recommended to use T. aureoviride URM 5158 and Pseudomonas sp. UAGF 14 in lettuce crops, because improved lettuce production, improves the biochemical quality of soils measured by absolute and specific enzymatic activities per unit of microbial biomass.

ABSTRACT Plant growth promoter microorganisms have been studied as important tools for increasing crop production. Lettuce is the most consumed hardwood crop in the world. Numerous microorganisms are capable of acting in a beneficial way in the growth of this culture. The objective of the present study was to evaluate the efficacy of Trichoderma and Pseudomonas on the microbial biomass, enzymatic activities in sandy soil and lettuce production. The experimental design was completely randomized with ten replicates and treatments: CONT (absolute control); CM (control with cattle manure fertilization); CMB (with fertilization and Pseudomonas sp.); CMF (with fertilization and T. aureoviride) and CMBF (with fertilization and the two microorganisms combined). The fertilizer used was organic with cattle manure in a dose recommended for the culture. This study evaluated the production of lettuce, microbial biomass and the enzymatic activity of acid phosphatase, alkaline phosphatase and urease. The combined application of CMBF was efficient in increasing lettuce production, because it increased 85% of the cv. Veronica cultivated on sandy soil. The combined use of plant growth promoting microorganisms resulted to an increase in microbial biomass. In lettuce crops, it is recommended to use T. aureoviride URM 5158 and Pseudomonas sp. UAGF 14 in lettuce crops, because improved lettuce production, improves the biochemical quality of soils measured by absolute and specific enzymatic activities per unit of microbial biomass. | RESUMO Os micro-organismos promotores do crescimento de plantas vêm sendo estudados como uma importante ferramenta para o aumento da produção agrícola. Lactuca sativa (alface) é a hortaliça folhosa mais consumida em todo mundo. Inúmeros micro-organismos podem atuar de forma benéfica no crescimento desta cultura. O objetivo do presente trabalho foi avaliar a eficácia de isolados de Trichoderma e Pseudomonas sp. na biomassa microbiana, atividades enzimáticas em solo arenoso e produção da alface cultivada com alface. O experimento foi conduzido em delineamento experimental inteiramente casualizado com dez repetições, sendo os tratamentos: CONT (controle absoluto); CM (controle apenas com a fertilização); CMB (com fertilização e Pseudomonas sp.); CMF (com fertilização e T. aureoviride) e CMBF (com fertilização e os dois micro-organismos combinados). A fertilização utilizada foi a orgânica pela adição de esterco bovino em dose recomendada para a cultura. Avaliou-se a produção de alface, a biomassa microbiana e a atividade enzimática da fostafase ácida, fosfatase alcalina e urease. A aplicação CMBF foi eficiente para o aumento na produção de alface, pois incrementou 85% da produção da cv. Verônica em solo arenoso. O uso combinado de micro-organismos promotores de crescimento de plantas incrementam a biomassa microbiana. Recomenda-se o uso de T. aureoviride URM 5158 e Pseudomonas sp. UAGF 14 no cultivo de alface, pois além de melhorar a produção, melhora a qualidade bioquímica dos solos mensurados por atividades enzimáticas absolutas e específicas por unidade de biomassa microbiana.

The adoption of measures to prevent and control erosive processes requires information about the factors affecting the erosion and the sediment transport conditions. However, the sediment yield of a basin depends on the availability of eroded material and the sediment transport capacity. Thus, the objective of this study was to identify the factors that affect the sediment transport capacity at different spatial scales in an area with caatinga vegetation. The study was carried out in the Iguatu Experimental Basin, in the state of Ceará, Brazil, from 2009 to 2014, by monitoring two scale levels: watershed with 2.06 ha, and erosion plot with 20 m². The variables evaluated for the rainfall events were precipitation, intensity of rainfall, antecedent soil moisture, precipitation of the antecedent five days, consecutive dry days, peak flow, runoff depth, and sediment yield. During the study period, 263 rainfall events (>2 mm) and 86 events generating runoff were recorded. Three Principal Components (PC) were developed using the Principal Component Analysis, which explained more than 79% of the total variance. Variables connected to the kinetic energy capacity of the rainfall to disaggregate soil particles, the energy for sediment transport, and the soil water content were framed in the CP1, CP2, and CP3, respectively. In the evaluated scales, the sediment yield presented a high correlation with the runoff depth, which indicates limiting conditions for sediment yield by the mass flow energy.

ABSTRACT The adoption of measures to prevent and control erosive processes requires information about the factors affecting the erosion and the sediment transport conditions. However, the sediment yield of a basin depends on the availability of eroded material and the sediment transport capacity. Thus, the objective of this study was to identify the factors that affect the sediment transport capacity at different spatial scales in an area with caatinga vegetation. The study was carried out in the Iguatu Experimental Basin, in the state of Ceará, Brazil, from 2009 to 2014, by monitoring two scale levels: watershed with 2.06 ha, and erosion plot with 20 m². The variables evaluated for the rainfall events were precipitation, intensity of rainfall, antecedent soil moisture, precipitation of the antecedent five days, consecutive dry days, peak flow, runoff depth, and sediment yield. During the study period, 263 rainfall events (>2 mm) and 86 events generating runoff were recorded. Three Principal Components (PC) were developed using the Principal Component Analysis, which explained more than 79% of the total variance. Variables connected to the kinetic energy capacity of the rainfall to disaggregate soil particles, the energy for sediment transport, and the soil water content were framed in the CP1, CP2, and CP3, respectively. In the evaluated scales, the sediment yield presented a high correlation with the runoff depth, which indicates limiting conditions for sediment yield by the mass flow energy. | RESUMO A adoção de medidas preventivas ou de controle dos processos erosivos requer um entendimento não somente dos fatores que influenciam a erosão, mas também das condições de transporte. Contudo a produção de sedimentos de uma bacia é função da disponibilidade de material erodido e da capacidade de transporte. Assim, objetivou-se com esse estudo identificar os fatores relacionados com a capacidade de transporte de sedimentos em área com vegetação de caatinga, em escalas espaciais distintas. O estudo foi desenvolvido na Bacia Experimental de Iguatu, Ceará, com monitoramento nas escalas de microbacia com 2,06 ha e parcela de 20 m2. As variáveis investigadas no período de 2009 a 2014 foram: altura pluviométrica, intensidades das chuvas, umidade antecedente do solo, precipitação antecedente dos últimos 5 dias, dias consecutivos secos, vazão de pico, lâmina escoada e produção de sedimentos. No período de estudo foram registrados 263 eventos de chuvas (> 2 mm) e 86 eventos geradores de escoamento. Com auxílio de Análises das Componentes Principais, foram formadas três Componentes Principais (CP) explicando mais de 79 % da variância total. Nas CP1, CP2 e CP3 foram enquadradas, respectivamente, variáveis relacionadas ao poder energético da chuva em desagregar partículas de solo; a energia para o transporte de sedimentos e ao conteúdo de água no solo. Nas escalas estudadas a produção de sedimentos apresentou elevada correlação com a lâmina escoada, indicando condições limitantes de produção de sedimento pela energia do fluxo de massa.

The objective of this study was to quantify the necromass stock in a Mixed Ombrophilous Forest (MOF) fragment in the National Forest of Irati, State of Paraná, Brazil. Two sampling methods were tested: FA1, consisting of a fixed area (FA) approach with sample units measuring 2,500 m2 (50 m × 50 m); and FA2, consisting of fixed area sampling units measuring 500 m2 (10 m × 50 m) and line intercept sampling (LI) using 50 m lines. Data were collected on permanent sample plots installed in the area, consisting of 25 square blocks of 1 ha. Fallen dead wood pieces with a diameter = 10 cm were used in the analysis. The dead wood was classified into three degrees of decomposition, and masses were calculated as the corresponding density at each class. The tested sampling methods were evaluated using coefficient of variation and relative sampling error, and the nonparametric Kruskal-Wallis test was used to compare the results between the methods. Volume size of fallen dead wood did not statistically differ between the methods, but variation in necromass volume was lower in the FA1 method, whereas the FA2 method had a smaller sampling error. Overall sampling error ranged from 23.4-27.92%; lowering the sampling error to 15% would require a high sampling intensity (FA1: 42 area units a.u., FA2: 99 a.u., and LI: 236 a.u.). Total necromass weights amounted to 4.67 Mg.ha-1 (FA1); 5.16 Mg.ha-1 (FA2) and 4.58 Mg.ha-1 (IL), and carbon stock estimates were 2.00 Mg.C.ha-1 (FA1); 2.20 Mg.C.ha-1 (FA2) and 1.96 Mg.C.ha-1 (IL).

ABSTRACT The objective of this study was to quantify the necromass stock in a Mixed Ombrophilous Forest (MOF) fragment in the National Forest of Irati, State of Paraná, Brazil. Two sampling methods were tested: FA1, consisting of a fixed area (FA) approach with sample units measuring 2,500 m2 (50 m × 50 m); and FA2, consisting of fixed area sampling units measuring 500 m2 (10 m × 50 m) and line intercept sampling (LI) using 50 m lines. Data were collected on permanent sample plots installed in the area, consisting of 25 square blocks of 1 ha. Fallen dead wood pieces with a diameter = 10 cm were used in the analysis. The dead wood was classified into three degrees of decomposition, and masses were calculated as the corresponding density at each class. The tested sampling methods were evaluated using coefficient of variation and relative sampling error, and the nonparametric Kruskal-Wallis test was used to compare the results between the methods. Volume size of fallen dead wood did not statistically differ between the methods, but variation in necromass volume was lower in the FA1 method, whereas the FA2 method had a smaller sampling error. Overall sampling error ranged from 23.4-27.92%; lowering the sampling error to 15% would require a high sampling intensity (FA1: 42 area units [a.u.], FA2: 99 a.u., and LI: 236 a.u.). Total necromass weights amounted to 4.67 Mg.ha-1 (FA1); 5.16 Mg.ha-1 (FA2) and 4.58 Mg.ha-1 (IL), and carbon stock estimates were 2.00 Mg.C.ha-1 (FA1); 2.20 Mg.C.ha-1 (FA2) and 1.96 Mg.C.ha-1 (IL). | RESUMO O estudo teve por objetivo quantificar o estoque de necromassa, em um fragmento de Floresta Ombrófila Mista, na Floresta Nacional de Irati, estado do Paraná. Dois métodos de amostragem foram testados, sendo área fixa (AF) com unidades amostrais de 2500 m2 (50 m x 50 m) -AF1; área fixa com unidades amostrais de 500 m2 (10 m x 50 m) - AF2 e método de amostragem por Linha Interceptadora (LI), com linhas de 50 m. Os dados foram coletados em parcelas permanentes instaladas na área, constituídas por 25 blocos quadrados de 1 ha. Foram medidas peças de madeira morta caída com diâmetro = 10 cm. A madeira morta foi classificada em três graus de decomposição e a massa foi calculada conforme a densidade correspondente a cada classe. Os métodos de amostragem testados foram avaliados por meio do Coeficiente de Variação e do Erro de Amostragem Relativo. Para comparações dos resultados entre os métodos utilizou-se o teste não paramétrico de Kruskal-Wallis. Os volumes para madeira morta caída não diferiram estatisticamente entre os métodos, porém o método AF1 teve menor variação do volume de necromassa e o método AF2 apresentou menor erro de amostragem. O erro amostral variou de 23,4 % a 27,92 % entre os métodos. Para atingir um erro de amostragem de 15% seria necessária uma elevada intensidade amostral (AF1: 42 unidades de área [u.a.], AF2: 99 u.a., e LI: 236 u.a.). O peso total de necromassa foi de 4,67 Mg.ha-1 (AF1); 5,16 Mg.ha-1 (AF2), e 4,58 Mg.ha-1 (LI) e o estoque de carbono foi de 2,00 Mg.C.ha-1(AF1); 2,20 Mg.C.ha-1 (AF2), e 1,96 Mg.C.ha-1 (LI).

The most-used preparation process of protein isolates (PI) involves the isoelectric precipitation of the protein. Heating shortens the preparation time but this procedure may affect the purity, yield, molecular profile of the protein, and the activity of the trypsin inhibitor. This study aimed to investigate the effect of heating in the production of cowpea protein isolates. Crude whole beans (WB) were defatted with hexane, and the protein isolates obtained by isoelectric precipitation with (HPI), and without (NHPI) heating. The protein content of the WB and the PI was determined by the micro-Kjeldahl method, and the extraction yield estimated from the protein content at the end of extraction in relation to this content in the raw material. Possible losses of protein fractions were followed by SDS-PAGE, and the trypsin inhibitor activity determined by an enzymatic assay (BAPNA: benzoyl-DL-arginine-p-nitroanilide). Protein content in HPI was 83.3%, less than in the NHPI (92.2%). The HPI yield was lower (40.0%) as compared to the NHPI (42.3%). Electrophoresis indicated bands ranging from 13 to 262 kDa in WB; and the NHPI presented a protein fraction’s profile closer to that of the WB than to the HPI. The WB had the trypsin inhibitor activity, expressed as Trypsin Inhibitory Units (TIU), of 32.5±0.5 TIU /mg-protein; HPI showed 12.7±0.5 TIU /mg-protein (39% of that observed in WB) and the NHPI, 8.3±0.2 TIU /mg-protein (25.5%). Heating reduces the yield and purity of proteins in the isolates. However, the inhibitory activity of trypsin cowpea is most affected by the isolation procedure.

ABSTRACT The most-used preparation process of protein isolates (PI) involves the isoelectric precipitation of the protein. Heating shortens the preparation time but this procedure may affect the purity, yield, molecular profile of the protein, and the activity of the trypsin inhibitor. This study aimed to investigate the effect of heating in the production of cowpea protein isolates. Crude whole beans (WB) were defatted with hexane, and the protein isolates obtained by isoelectric precipitation with (HPI), and without (NHPI) heating. The protein content of the WB and the PI was determined by the micro-Kjeldahl method, and the extraction yield estimated from the protein content at the end of extraction in relation to this content in the raw material. Possible losses of protein fractions were followed by SDS-PAGE, and the trypsin inhibitor activity determined by an enzymatic assay (BAPNA: benzoyl-DL-arginine-p-nitroanilide). Protein content in HPI was 83.3%, less than in the NHPI (92.2%). The HPI yield was lower (40.0%) as compared to the NHPI (42.3%). Electrophoresis indicated bands ranging from 13 to 262 kDa in WB; and the NHPI presented a protein fraction’s profile closer to that of the WB than to the HPI. The WB had the trypsin inhibitor activity, expressed as Trypsin Inhibitory Units (TIU), of 32.5±0.5 TIU /mg-protein; HPI showed 12.7±0.5 TIU /mg-protein (39% of that observed in WB) and the NHPI, 8.3±0.2 TIU /mg-protein (25.5%). Heating reduces the yield and purity of proteins in the isolates. However, the inhibitory activity of trypsin cowpea is most affected by the isolation procedure. | RESUMO O processo mais utilizado na preparação de isolado proteico (IP) envolve a precipitação isoelétrica da proteína. O aquecimento abrevia o tempo de preparo dos isolados. Entretanto, esse procedimento pode afetar a pureza, rendimento, perfil molecular da proteína e presença do inibidor de tripsina. Este trabalho objetivou investigar o efeito do aquecimento na produção de IP de feijão-caupi. O feijão integral (FI) cru foi moído e desengordurado com hexano e o IP foi obtido por precipitação isoelétrica com aquecimento (IPCA) e sem aquecimento (IPSA). Determinou-se o teor proteico do FI, e dos isolados pelo método de micro-Kjeldahl. Estimou-se o rendimento da extração, pela massa de proteína ao final da extração em relação à existente inicialmente no material seco. Possíveis perdas de frações proteicas foram avaliadas por eletroforese SDS-PAGE. A atividade inibitória da tripsina foi avaliada em ensaio enzimático (BAPNA: benzoil-DLarginina-p-nitroanilida). Obteve-se um IPCA (83,3% de proteína), menos puro que o IPSA (92,2%). O rendimento do IPCA foi menor (40,0%) em relação ao IPSA (42,3%). A eletroforese indicou bandas variando de 13 a 262 kDa no FI, o IPSA apresentou um perfil de frações proteicas mais próximo ao FI que o IPCA. O FI apresentou atividade inibitória, expressa em Unidades Inibitórias de Tripsina (UIT) de 32,5±0,5UIT/mg-proteína, o IPCA apresentou 12,7±0,5UIT/mg-proteína (39% do observado no FI) e, o IPSA, 8,3±0,2 UIT/mg-proteína (25,5%). O aquecimento reduz o rendimento e a pureza da proteína nos isolados. Entretanto, a atividade inibitória da tripsina é mais afetada pelo processo de isolamento.

This study evaluated the effects of feeding frequency and feeding rate on productive performance of hybrid surubims reared in cages with automatic feeding. Three feeding frequencies (6, 12 and 24 meals day-1), and two feeding rates (4% and 8% of body weight per day, BW day-1) were evaluated. Hybrid surubims (Pseudoplatystoma corruscans × Pseudoplatystoma fasciatum) with average weights of 24.20 ± 0.80 g were distributed in 18 cages of 1.0 m³ at a density of 60 fish m-3. Fish were fed commercial ration containing 40% crude protein, and feeding took place at night using automatic feeders. The study lasted 47 days. With a feeding rate of 4% BW day-1, the final weight (FW) of the surubims was higher when feed was supplied as 24 meals day-1 (66.92 g). However, using 8% BW day-1, the FW was greater with a feeding frequency of 12 meals day-1 (106.90 g). Regardless of feed frequency, the values of daily weight gain and standard length of surubims were higher with the feeding rate of 8% BW day-1. Therefore, the feeding rate of 8% BW day-1, provided in 12 meals day-1, provided better productive performance for hybrid surubims (Pseudoplatystoma corruscans × Pseudoplatystoma fasciatum) reared in cages.

RESUMO Este estudo avaliou os efeitos da frequência e taxa de alimentação sobre o desempenho produtivo de surubins híbridos criados em tanque rede. Três frequências (6, 12 e 24 refeições dia-1) e duas taxas de alimentação (4% e 8% do peso vivo por dia, PV dia-1) foram avaliadas. Híbridos de surubim (Pseudoplatystoma corruscans x Pseudoplatystoma fasciatum), com peso médio de 24,20 ± 0,80 g, foram distribuídos em 18 tanques rede de 1,0 m³ com densidade de 60 peixes m-³. Os peixes foram alimentados no período noturno por alimentadores automáticos, com ração comercial contendo 40% de proteína bruta. O estudo teve duração de 47 dias. Com a frequência de 4% PV dia-1, o peso final (PF) dos surubins foi maior com o fornecimento da ração em 24 refeições dia-1 (66,92 g). Entretanto, usando 8% PV dia-1 o PF foi maior com a frequência de 12 refeições dia-1 (106,90 g). Independente da frequência alimentar, os valores de ganho de peso diário e comprimento padrão dos surubins foram maiores com a taxa de alimentação de 8% PV dia-1. Assim, o fornecimento da ração com taxa alimentar de 8% PV dia-1, dividida em 12 refeições dia-1, proporcionou melhor desempenho produtivo para surubins híbridos (Pseudoplatystoma corruscans × Pseudoplatystoma fasciatum) criados em tanque rede. | ABSTRACT This study evaluated the effects of feeding frequency and feeding rate on productive performance of hybrid surubims reared in cages with automatic feeding. Three feeding frequencies (6, 12 and 24 meals day-1), and two feeding rates (4% and 8% of body weight per day, BW day-1) were evaluated. Hybrid surubims (Pseudoplatystoma corruscans × Pseudoplatystoma fasciatum) with average weights of 24.20 ± 0.80 g were distributed in 18 cages of 1.0 m³ at a density of 60 fish m-3. Fish were fed commercial ration containing 40% crude protein, and feeding took place at night using automatic feeders. The study lasted 47 days. With a feeding rate of 4% BW day-1, the final weight (FW) of the surubims was higher when feed was supplied as 24 meals day-1 (66.92 g). However, using 8% BW day-1, the FW was greater with a feeding frequency of 12 meals day-1 (106.90 g). Regardless of feed frequency, the values of daily weight gain and standard length of surubims were higher with the feeding rate of 8% BW day-1. Therefore, the feeding rate of 8% BW day-1, provided in 12 meals day-1, provided better productive performance for hybrid surubims (Pseudoplatystoma corruscans × Pseudoplatystoma fasciatum) reared in cages.

Changes in the agricultural environment can be determined by providing microbiological indicators of the soil since the soil microorganisms are sensitive to variations in the environment. In this way, the impact of herbicides of long residual effect on the rhizospheric soil microorganisms of sugarcane cultivars was evaluated. The cultivars of sugarcane (SP 81-3250 and RB 867515) were treated with four herbicides (tebuthiuron, diuron, ametryn and mixture of diuron + and hexazinone) applied in pre-emergence. The herbicides were applied seven days after the planting of the gems. At 30, 60 and 90 days after the application, the soil rhizosphere was collected in each treatment to determine the CO2 evolution of the soil (C-CO2), microbial biomass carbon (MBC), metabolic quotient (qCO2), solubility potential of inorganic phosphorus P (PSFI) and percentage of roots colonized by mycorrhizal fungi Arbuscular. No changes were observed in the microbial activity of the sugarcane rhizosphere at 30 days after application of the herbicides (DAA). However, at 90 DAA, all herbicides negatively affected the activity of the rhizospheric microorganisms of sugarcane. The metabolic activity of rhizosphere in soil cultivated with RB 867515 was less affected by herbicides.

ABSTRACT Changes in the agricultural environment can be determined by providing microbiological indicators of the soil since the soil microorganisms are sensitive to variations in the environment. In this way, the impact of herbicides of long residual effect on the rhizospheric soil microorganisms of sugarcane cultivars was evaluated. The cultivars of sugarcane (SP 81-3250 and RB 867515) were treated with four herbicides (tebuthiuron, diuron, ametryn and mixture of diuron + and hexazinone) applied in pre-emergence. The herbicides were applied seven days after the planting of the gems. At 30, 60 and 90 days after the application, the soil rhizosphere was collected in each treatment to determine the CO2 evolution of the soil (C-CO2), microbial biomass carbon (MBC), metabolic quotient (qCO2), solubility potential of inorganic phosphorus P (PSFI) and percentage of roots colonized by mycorrhizal fungi Arbuscular. No changes were observed in the microbial activity of the sugarcane rhizosphere at 30 days after application of the herbicides (DAA). However, at 90 DAA, all herbicides negatively affected the activity of the rhizospheric microorganisms of sugarcane. The metabolic activity of rhizosphere in soil cultivated with RB 867515 was less affected by herbicides. | RESUMO Alterações no ambiente agrícola podem ser determinadas por meio indicadores microbiológicos do solo, uma vez que a microbiota do solo é sensível às modificações do ambiente. Desta forma, avaliou-se o impacto de herbicidas de longo efeito residual sobre a microbiota rizosférica de cultivares de cana-de-açúcar. As cultivares de cana-de-açúcar SP 81-3250 e RB 867515 foram tratadas com quatro herbicidas (tebuthiuron, diuron, ametryn e a mistura diuron + hexazinone) aplicados em pré-emergência. Os herbicidas foram aplicados sete dias após o plantio das gemas. Aos 30, 60 e 90 dias após a aplicação, o solo rizosferico de cada tratamento foi coletado para determinar a evolução de CO2 no solo (C-CO2), o carbono da biomassa microbiana (MBC), o quociente metabólico (qCO2), o potencial de solubilização de P inorgânico (PSFI) e a percentagem de raízes colonizadas por fungos micorrízicos arbusculares. Não foram observadas alterações na atividade microbiana da rizosfera da cana-de-açúcar aos 30 dias após a aplicação dos herbicidas (DAA). No entanto, aos 90 DAA todos os herbicidas afetaram negativamente a atividade dos micro-organismos rizosféricos da cana-de-açúcar. A atividade metabólica da rizosfera em solo cultivado com a RB 867515 foi menos afetada pelos herbicidas.