Passiflora cincinnata Mast. has become more popular in the market because the unusual flavor of its fruits and natural beauty of its flowers, and has great potential for breeding programs of Passiflora edulis f. flavicarpa, because its resistance to diseases and drought. The objective of this work was to evaluate seven wild passion fruit (P. cincinnata) accessions, using morphological and agronomic descriptors and molecular markers type ISSR, to identify their morphoagronomic and genetic variabilities and potential for use in breeding programs. A randomized block experimental design was used with five replications and two plants per plot. Thirteen qualitative and twenty-one quantitative, vegetative and floral characteristics were used for morphoagronomic characterization. Twelve ISSR primers were evaluated for molecular characterization. Among the qualitative characteristics, only the color variations were significantly different between the accessions. According to the mean squares of the quantitative characteristics evaluated, obtained from analysis of variance, the means of accessions showed significant differences (p<0.01) for all characteristics. The IAL (internode average length) was the morphological descriptor that most contributed to diversity, with 43.12%, followed by DH5 (stem diameter at 5 cm height) and SW (sepal width). The average genetic similarity found was 68%. Despite the low genetic variability found among accessions, the primers UBC-887 and UBC-841 stood out with high percentage of polymorphism with 14 and 11 polymorphic fragments, respectively, and higher values of polymorphism information content (PIC), resolving power (RP) and marker index (MI), denoting suitability for use in diversity studies of P. cincinnata. Low variability was found among accessions evaluated.

ABSTRACT Passiflora cincinnata Mast. has become more popular in the market because the unusual flavor of its fruits and natural beauty of its flowers, and has great potential for breeding programs of Passiflora edulis f. flavicarpa, because its resistance to diseases and drought. The objective of this work was to evaluate seven wild passion fruit (P. cincinnata) accessions, using morphological and agronomic descriptors and molecular markers type ISSR, to identify their morphoagronomic and genetic variabilities and potential for use in breeding programs. A randomized block experimental design was used with five replications and two plants per plot. Thirteen qualitative and twenty-one quantitative, vegetative and floral characteristics were used for morphoagronomic characterization. Twelve ISSR primers were evaluated for molecular characterization. Among the qualitative characteristics, only the color variations were significantly different between the accessions. According to the mean squares of the quantitative characteristics evaluated, obtained from analysis of variance, the means of accessions showed significant differences (p<0.01) for all characteristics. The IAL (internode average length) was the morphological descriptor that most contributed to diversity, with 43.12%, followed by DH5 (stem diameter at 5 cm height) and SW (sepal width). The average genetic similarity found was 68%. Despite the low genetic variability found among accessions, the primers UBC-887 and UBC-841 stood out with high percentage of polymorphism with 14 and 11 polymorphic fragments, respectively, and higher values of polymorphism information content (PIC), resolving power (RP) and marker index (MI), denoting suitability for use in diversity studies of P. cincinnata. Low variability was found among accessions evaluated. | RESUMO A espécie Passiflora cincinnata Mast. vem se popularizando no mercado pelo sabor incomum dos seus frutos, beleza natural de sua flores e possui grande potencial para a cultura de Passiflora edulis f. flavicarpa, pois apresenta resistência a doenças e déficit hídrico. Este trabalho teve como objetivo avaliar sete acessos de maracujá-do-mato (P. cincinnata) por meio de descritores morfológicos, descritores agronômicos e marcadores moleculares do tipo ISSR visando identificar variabilidade morfoagronômica e genética e o potencial para serem utilizados em programas de melhoramento. O delineamento experimental foi em blocos casualizados, com cinco repetições e duas plantas por parcela. Para caracterização morfoagronômica foram avaliadas 13 características qualitativas e 21 características quantitativas vegetativas e florais. Para caracterização molecular foram testados 12 primers de ISSR. Entre as características qualitativas apenas as variações de coloração apresentaram diferenças marcantes entre os diferentes acessos. De acordo com os quadrados médios obtidos das análises de variância para as características quantitativas avaliadas pode-se ressaltar as diferenças significativas (p<0,01) entre as médias dos acessos para todos os caracteres avaliados. Verificou-se que para os 21 descritores morfológicos avaliados, o que mais contribuiu para a diversidade foi o MI (média internódio) com 43,12%, seguido por DH5 (diâmetro das hastes a 5 centímetros do solo) e LS (largura da sépala). A similaridade genética média encontrada foi 68%. Apesar de ser diagnosticada baixa variabilidade genética entre os acessos avaliados, os primers UBC-887 e UBC-841 se destacaram com alto percentual de polimorfismo, com 14 e 11 fragmentos polimórficos respectivamente e valores altos para conteúdo da informação de polimorfismo (PIC), poder de resolução do primer (RP) e índice do marcador (MI) dos primers, demonstrando aptidão para serem utilizados em pesquisas de diversidade em P. cincinnata. Foi diagnosticada baixa variabilidade entre os acessos avaliados.

Banana farming is an activity of great economic and social importance, and is carried out in most tropical countries. The aim of this work was to evaluate the biomass accumulation and levels of nitrogen (N), phosphorus (P), potassium (K), calcium (Ca) and magnesium (Mg) in micropropagated plants of the banana “Prata Catarina” during the acclimatization phase, under different types and doses of biofertilisers. The experimental design included randomised blocks in a 2 × 5 + (2) factorial scheme, with two types of liquid biofertilisers (bovine biofertiliser with anaerobic and aerobic fermentation) and five biofertiliser doses (0.25, 0.50, 0.75, 1.00, and 1.25 L plant-1 week-1), as well as two additional treatments (control and recommended mineral fertilisation). The following variables were analysed: dry weight of the leaves and roots, and mineral element content (N, P, K, Ca, and Mg) in different parts of the plant (leaf and root). During 90 days of acclimatization, the nutritional contribution of bovine biofertiliser with anaerobic fermentation was greater in comparison with the biofertiliser with aerobic fermentation and the control, but lower in comparison with mineral fertilisation. The 1000-mL dose of the biofertiliser with anaerobic fermentation promoted greater dry weight accumulation in the leaves and roots of the banana “Prata Catarina”. The biofertiliser with anaerobic fermentation promoted higher levels of N, K, and Ca in the leaves, whereas the biofertiliser with aerobic fermentation promoted higher levels of P in the leaves and roots.

RESUMO A bananicultura destaca-se como uma atividade de grande importância econômica e social, sendo explorada na maioria dos países tropicais. O presente trabalho propôs avaliar o acúmulo de biomassa e os teores de nitrogênio (N), fósforo (P), potássio (K), cálcio (Ca) e magnésio (Mg) em mudas micropropagadas de bananeira cv. Prata Catarina, durante a fase de aclimatização sob diferentes tipos doses e de biofertilizantes. O delineamento experimental foi em blocos casualizados em arranjo fatorial 2 × 5 + (2), referentes a dois tipos de biofertilizantes líquidos (bovino de fermentação anaeróbia e aeróbia) e cinco doses de biofertilizantes (0,25; 0,50; 0,75; 1,00 e 1,25 L planta-1 semana-1), com dois tratamentos adicionais (controle e adubação mineral recomendada). Foram analisadas as seguintes variáveis: massa seca das folhas, das raízes e teores de elementos minerais (N, P, K, Ca e Mg) nas diferentes partes da planta (folha e raiz). O biofertilizante bovino com fermentação anaeróbia apresenta maior aporte nutricional em relação biofertilizante de fermentação aeróbio e ao controle, porém menor em relação à adubação mineral para as mudas durante os 90 dias de aclimatização. A dose de 1000 mL do biofertilizante com fermentação anaeróbia promove maior acúmulo de massa seca nas folhas e raízes da bananeira Prata Catarina. O biofertilizante com fermentação anaeróbia proporciona maiores teores de N, K e Ca nas folhas, enquanto, o biofertilizante com fermentação aeróbia promove maiores teores de P nas folhas e raízes. | ABSTRACT Banana farming is an activity of great economic and social importance, and is carried out in most tropical countries. The aim of this work was to evaluate the biomass accumulation and levels of nitrogen (N), phosphorus (P), potassium (K), calcium (Ca) and magnesium (Mg) in micropropagated plants of the banana “Prata Catarina” during the acclimatization phase, under different types and doses of biofertilisers. The experimental design included randomised blocks in a 2 × 5 + (2) factorial scheme, with two types of liquid biofertilisers (bovine biofertiliser with anaerobic and aerobic fermentation) and five biofertiliser doses (0.25, 0.50, 0.75, 1.00, and 1.25 L plant-1 week-1), as well as two additional treatments (control and recommended mineral fertilisation). The following variables were analysed: dry weight of the leaves and roots, and mineral element content (N, P, K, Ca, and Mg) in different parts of the plant (leaf and root). During 90 days of acclimatization, the nutritional contribution of bovine biofertiliser with anaerobic fermentation was greater in comparison with the biofertiliser with aerobic fermentation and the control, but lower in comparison with mineral fertilisation. The 1000-mL dose of the biofertiliser with anaerobic fermentation promoted greater dry weight accumulation in the leaves and roots of the banana “Prata Catarina”. The biofertiliser with anaerobic fermentation promoted higher levels of N, K, and Ca in the leaves, whereas the biofertiliser with aerobic fermentation promoted higher levels of P in the leaves and roots.

The objective of this work was to evaluate the effects of the application of phosphogypsum and vinasse on soil chemical properties and productivity and nutritional characteristics of alfalfa (Medicago sativa). The experiment was conducted in a randomized block design, using a 3×5 factorial arrangement, with three vinasse rates (0, 150 and 300 m3 ha-1) and five phosphogypsum rates (0, 3, 6, 9 and 12 Mg ha-1). The alfalfa chemical composition and shoot dry matter (SDW) and soil chemical properties (in the layers 0.0-0.2 and 0.21-0.4 m) were evaluated. The vinasse rates increased the soil potassium contents, while the phosphogypsum rates promoted linear increases in soil calcium and sulfur contents. The base saturation was increased and the magnesium content showed a quadratic response on the layer 0.21-0.4 m with the increase in phosphogypsum rates. The calcium, magnesium and phosphorus contents in the alfalfa leaves were lower with vinasse application. The phosphogypsum rates promoted linear increases in alfalfa SDW. Vinasse rated 150 m3 ha-1 was been enough to SDW increase. Calcium and magnesium contents in the leaves fitted a quadratic model, with maximum calcium content in the phosphogypsum rate of 9.5 Mg ha-1 and the minimum magnesium content in the phosphogypsum rate of 8.7 Mg ha-1. The leaf sulfur contents in all vinasse rates and leaf potassium contents in the highest vinasse rate showed maximum accumulation at near 9 Mg ha-1 of phosphogypsum.

RESUMO O objetivo do trabalho foi avaliar os efeitos da aplicação de gesso e vinhaça na produção de matéria seca, nutrição e atributos químicos do solo cultivado com alfafa. O experimento foi realizado em delineamento experimental de blocos ao acaso, em esquema fatorial 3 × 5, com três doses de vinhaça, 0, 150 e 300 m3 ha-1 e cinco doses de gesso agrícola (0, 3, 6, 9 e 12 Mg ha-1). Foram avaliados a composição química e matéria seca da parte aérea (MSPA) e os atributos químicos do solo (0-0,2 e 0,21-0,4 m). As doses de vinhaça aumentaram os teores de potássio, enquanto as doses de gesso promoveram acréscimos lineares nos teores de cálcio e enxofre no solo. A saturação de bases foi crescente e o magnésio teve efeito quadrático na camada de 0,21-0,4 m em função das doses de gesso. Os teores de cálcio, magnésio e fósforo na folha da alfafa foram menores com a aplicação de vinhaça. As doses de gesso promoveram acréscimos lineares na MSPA da alfafa. A dose de 150 m3 ha-1 já foi suficiente para aumentar a MSPA. Os teores de cálcio e magnésio na folha tiveram ajuste quadrático, com máximo acúmulo de cálcio em 9,5 Mg ha-1 e o magnésio com ponto de mínimo na dose de 8,7 Mg ha-1 de gesso. Os teores foliares de enxofre sob doses de vinhaça e os de potássio na maior dose obtiveram máximo acumulo na presença de 9 Mg ha-1 de gesso. | ABSTRACT The objective of this work was to evaluate the effects of the application of phosphogypsum and vinasse on soil chemical properties and productivity and nutritional characteristics of alfalfa (Medicago sativa). The experiment was conducted in a randomized block design, using a 3×5 factorial arrangement, with three vinasse rates (0, 150 and 300 m3 ha-1) and five phosphogypsum rates (0, 3, 6, 9 and 12 Mg ha-1). The alfalfa chemical composition and shoot dry matter (SDW) and soil chemical properties (in the layers 0.0-0.2 and 0.21-0.4 m) were evaluated. The vinasse rates increased the soil potassium contents, while the phosphogypsum rates promoted linear increases in soil calcium and sulfur contents. The base saturation was increased and the magnesium content showed a quadratic response on the layer 0.21-0.4 m with the increase in phosphogypsum rates. The calcium, magnesium and phosphorus contents in the alfalfa leaves were lower with vinasse application. The phosphogypsum rates promoted linear increases in alfalfa SDW. Vinasse rated 150 m3 ha-1 was been enough to SDW increase. Calcium and magnesium contents in the leaves fitted a quadratic model, with maximum calcium content in the phosphogypsum rate of 9.5 Mg ha-1 and the minimum magnesium content in the phosphogypsum rate of 8.7 Mg ha-1. The leaf sulfur contents in all vinasse rates and leaf potassium contents in the highest vinasse rate showed maximum accumulation at near 9 Mg ha-1 of phosphogypsum.

The proper soil use and management are essential for a sustainable production, thus the pursuit for systems that minimize damages to the environment and improve soil fertility is continuous. The objective of this study was to evaluate changes in soil fertility by the use and management of an Oxisol (Latossolo Vermelho - amarelo, SiBCS) in the Amazon biome. The soil uses and management systems evaluated were native forest, native pasture, minimum tillage system and conventional tillage system. The chemical atributes of the soil layers 0.00 - 0.10, 0.10 - 0.20, 0.20 - 0.30 and 0.30 - 0.40 m were evaluated. The soil pH (CaCl 2 ) and the contents of organic matter, available phosphorus and exchangeable potassium, calcium, magnesium and aluminum were evaluated and the saturation by Al, effective cation exchange capacity and soil potential were determined. The highest pH values were found in the native pasture area, and the highest exchangeable aluminum contents were found in the layers 0.00 - 0.10 and 0.10 - 0.20 m of the conventional tillage system. The highest phosphorus availability was found in the layer 0.00 - 0.10 m of the native forest area. The highest potassium availability was found in the minimum tillage system. The lowest calcium and magnesium values were found in the layers 0.00 - 0.10 and 0.10 - 0.20 m of the conventional tillage system. The highest soil organic matter content was found in the layer 0.00 - 0.10 m of the native forest area. The nutrient availability for plants decreases in soils managed using the conventional tillage system. Soil uses and management systems of little soil disturbance are recommended to minimize environmental degradation in the Amazon biome.

ABSTRACT The proper soil use and management are essential for a sustainable production, thus the pursuit for systems that minimize damages to the environment and improve soil fertility is continuous. The objective of this study was to evaluate changes in soil fertility by the use and management of an Oxisol (Latossolo Vermelho-amarelo, SiBCS) in the Amazon biome. The soil uses and management systems evaluated were native forest, native pasture, minimum tillage system and conventional tillage system. The chemical atributes of the soil layers 0.00-0.10, 0.10-0.20, 0.20-0.30 and 0.30-0.40 m were evaluated. The soil pH (CaCl2) and the contents of organic matter, available phosphorus and exchangeable potassium, calcium, magnesium and aluminum were evaluated and the saturation by Al, effective cation exchange capacity and soil potential were determined. The highest pH values were found in the native pasture area, and the highest exchangeable aluminum contents were found in the layers 0.00-0.10 and 0.10-0.20 m of the conventional tillage system. The highest phosphorus availability was found in the layer 0.00-0.10 m of the native forest area. The highest potassium availability was found in the minimum tillage system. The lowest calcium and magnesium values were found in the layers 0.00-0.10 and 0.10-0.20 m of the conventional tillage system. The highest soil organic matter content was found in the layer 0.00-0.10 m of the native forest area. The nutrient availability for plants decreases in soils managed using the conventional tillage system. Soil uses and management systems of little soil disturbance are recommended to minimize environmental degradation in the Amazon biome. | RESUMO O uso e manejo do solo adequados são fundamentais para a produção sustentável, sendo constante a busca por sistemas que minimizem os danos causados ao ambiente e que melhorem a fertilidade do solo. Objetivou-se avaliar as alterações nos atributos de fertilidade em decorrência do uso e manejo em Latossolo Vermelho-Amarelo (LVA) na Amazônia. Os seguintes sistemas de uso e manejo do solo num LVA foram avaliados: floresta nativa, pastagem nativa e áreas preparadas por sistema de cultivo mínimo e cultivo convencional. As avaliações dos atributos químicos do solo foram realizadas nas profundidades de 0 -0,10, 0,10-0,20, 0,20-0,30 e 0,30-0,40 m. Foram avaliados o pH do solo (CaCl2), o teor de matéria orgânica do solo, os teores de fósforo disponível e potássio trocável, cálcio, magnésio e alumino trocáveis, sendo ainda determinados a saturação por Al, a capacidade de troca catiônica efetiva e potencial do solo. Os valores de pH mais elevados foram verificados no sistema de pastagem nativa e os maiores teores de alumínio trocável nas profundidades de 0-0,10 e 0,10-0,20 m foram verificadas no sistema de cultivo convencional. O sistema de floresta nativa apresentou a maior disponibilidade de fósforo na profundidade de 0-0,10 m. As maiores disponibilidades de potássio foram verificadas no sistema de cultivo mínimo. Os menores valores de cálcio e magnésio nas profundidades de 0-0,10 e 0,10-0,20 m ocorreram no sistema de cultivo convencional. O sistema de floresta nativa apresentou o maior teor de matéria orgânica do solo na profundidade de 0-0,10 m. A disponibilidade de nutrientes para as plantas é reduzida em solos manejados em sistema de plantio convencional. Os sistemas de uso e manejo de menor revolvimento do solo são indicados para minimizar a degradação ambiental na Amazônia.

Weed interference is a factor that limits the productivity of beans and, among these, hairy beggarticks is one of the main species competing with the crop for environmental resources. Thus, the aim of this study is to evaluate the competitive ability of black bean cultivars (BRS Campeiro, IPR Uirapuru, SCS204 Predileto and BRS Supremo) in the presence of a biotype of hairy beggarticks. The experimental design is a completely randomized block with four replications. Treatments were arranged in a replacement series, consisting of a proportion of the crop and the hairy beggarticks: 100:0; 75:25; 50:50: 24:75, and 0:100, which corresponds to 40:0, 30:10, 20:20, 10:30, and 0:40 plant pots1. We accomplished competitive analysis through diagrams applied to the replacement series, as well as using relative competitive indices. The leaf area and shoot dry mass were evaluated at 40 days after emergence of the species. There was competition between bean cultivars and hairy beggarticks for the same environmental resources, causing negative interference in the growth of the species, independent of the proportion of plants. Bean cultivars had a lower relative loss by reducing the morphological variables of the hairy beggarticks, thereby demonstrating superiority in its competitive ability in relation to the weed. Interspecific competition is less damaging than intraspecific competition for both species.

RESUMO A interferência causada pelas plantas daninhas é um dos fatores que limitam a produtividade do feijoeiro e dentre essas destaca-se o picão-preto como uma das principais espécies que competem com a cultura pelos recursos do meio. Assim sendo, objetivou-se com este trabalho, avaliar a habilidade competitiva de cultivares de feijão do tipo preto (BRS Campeiro, IPR Uirapuru, SCS204 Predileto e BRS Supremo) na presença de biótipo de picão-preto. O delineamento utilizado foi o de blocos casualizados, com quatro repetições. Os tratamentos foram arranjados em série substitutiva e constituíram-se de proporções da cultura e de picão-preto: 100:0; 75:25; 50:50: 24:75 e 0:100, o que corresponde a 40:0, 30:10, 20:20, 10:30 e 0:40 plantas vaso-1. A análise da competitividade foi efetuada por meio de diagramas aplicados a experimentos substitutivos, mais uso de índices de competitividade relativa. Avaliou-se a área foliar e a massa seca da parte aérea aos 40 dias após a emergência das espécies. Houve competição pelos mesmos recursos do meio entre as cultivares de feijão e o picão-preto, ocasionando interferência negativa no crescimento das espécies, independentemente da proporção de plantas. As cultivares de feijão apresentaram menor perda relativa ao reduzirem as variáveis morfológicas do picão-preto e demonstram possuir superioridade na habilidade competitiva em relação à planta daninha. A competição interespecífica é menos prejudicial para ambas as espécies envolvidas do que a competição intraespecífica. | ABSTRACT Weed interference is a factor that limits the productivity of beans and, among these, hairy beggarticks is one of the main species competing with the crop for environmental resources. Thus, the aim of this study is to evaluate the competitive ability of black bean cultivars (BRS Campeiro, IPR Uirapuru, SCS204 Predileto and BRS Supremo) in the presence of a biotype of hairy beggarticks. The experimental design is a completely randomized block with four replications. Treatments were arranged in a replacement series, consisting of a proportion of the crop and the hairy beggarticks: 100:0; 75:25; 50:50: 24:75, and 0:100, which corresponds to 40:0, 30:10, 20:20, 10:30, and 0:40 plant pots1. We accomplished competitive analysis through diagrams applied to the replacement series, as well as using relative competitive indices. The leaf area and shoot dry mass were evaluated at 40 days after emergence of the species. There was competition between bean cultivars and hairy beggarticks for the same environmental resources, causing negative interference in the growth of the species, independent of the proportion of plants. Bean cultivars had a lower relative loss by reducing the morphological variables of the hairy beggarticks, thereby demonstrating superiority in its competitive ability in relation to the weed. Interspecific competition is less damaging than intraspecific competition for both species.

The aim of this work was to perform botanical identification and to estimate genetic diversity in two sequential inbred generations (progenies S1 and S2) of melon accessions from traditional agriculture in the state of Maranhão, in order to generate useful information for commercial melon breeding. Two field experiments were carried out in a completely randomized block, using four replicates of 15 accessions from a first selfing cycle in 2013, and three replicates of 25 subaccessions (generation S2) in 2014. Flower and fruit descriptors were measured to obtain quantitative and qualitative data, in addition to a systematized photographic documentation of fruit for visually comparing the progenies S1 and S2. Distance matrices for quantitative and qualitative data were obtained and used to perform a joint analysis and UPGMA method. Large genetic diversity was found in the accessions analysed, since the presence of melon progenies was observed in the Cucumis melo ssp. agrestis, with its botanical varieties momordica and conomom, and of the Cucumis melo ssp. melo, with the botanical varieties cantalupensis and chandalak. Divergence analysis showed the formation of three groups in generation S1 and four groups in S2. However, the groups were not separated either by subspecies or by botanical variety. Thus, in addition to the large genetic diversity among and within melon accessions from family farming in the state of Maranhão, the progenies presented a large introgression of traits of the different subspecies and their botanical varieties due to the reproductive system and seed management of these species.

RESUMO O trabalho teve como objetivos realizar a classificação botânica e estimar a diversidade genética em duas gerações endogâmicas sequenciais (progênies S1 e S2) de acessos de melão da agricultura familiar do Maranhão, visando gerar informações úteis para o melhoramento do melão comercial. Foram conduzidos dois experimentos de campo em blocos casualizados completos, com quatro repetições e 15 acessos em 2013 e, três repetições e 25 subacessos em 2014. Para avaliação dos acessos foram aplicados descritores quantitativos e qualitativos de flor e fruto, além de uma documentação fotográfica sistematizada dos frutos com análise visual, comparando os frutos das gerações S1 e S2. Foram obtidas as matrizes de distância nos dois tipos de descritores e se fez a análise conjunta e em seguida realizou-se o agrupamento pelo método UPGMA. Verificou-se que nos acessos analisados, existe uma grande diversidade genética, pois foram encontradas progênies de melão da subespécie agrestis e suas variedades botânicas momordica e conomom e da subespécie melo com as variedades botânicas cantalupensis e chandalak. A análise de divergência na geração S1 mostrou a formação de três grupos e na geração S2 foram formados quatro agrupamentos, entretanto os grupos não foram formados nem por subespécie nem por variedade botânica. Assim, além de se ter encontrado uma grande diversidade genética entre e dentro dos acessos de melão da agricultura familiar maranhense, é provável que tenha ocorrido grande introgressão de alelos das subespécies e das diferentes variedades botânicas na área dos agricultores devido ao sistema reprodutivo da espécie a ao manejo de sementes. | ABSTRACT The aim of this work was to perform botanical identification and to estimate genetic diversity in two sequential inbred generations (progenies S1 and S2) of melon accessions from traditional agriculture in the state of Maranhão, in order to generate useful information for commercial melon breeding. Two field experiments were carried out in a completely randomized block, using four replicates of 15 accessions from a first selfing cycle in 2013, and three replicates of 25 subaccessions (generation S2) in 2014. Flower and fruit descriptors were measured to obtain quantitative and qualitative data, in addition to a systematized photographic documentation of fruit for visually comparing the progenies S1 and S2. Distance matrices for quantitative and qualitative data were obtained and used to perform a joint analysis and UPGMA method. Large genetic diversity was found in the accessions analysed, since the presence of melon progenies was observed in the Cucumis melo ssp. agrestis, with its botanical varieties momordica and conomom, and of the Cucumis melo ssp. melo, with the botanical varieties cantalupensis and chandalak. Divergence analysis showed the formation of three groups in generation S1 and four groups in S2. However, the groups were not separated either by subspecies or by botanical variety. Thus, in addition to the large genetic diversity among and within melon accessions from family farming in the state of Maranhão, the progenies presented a large introgression of traits of the different subspecies and their botanical varieties due to the reproductive system and seed management of these species.

The objective of this work was to evaluate the effect of plant densities and sidedressed nitrogen (N) rates on nutrition and productive performance of the common bean cultivars IPR 139 and Pérola. For each cultivar, a randomized complete block experimental design was used in a split-plot arrangement, with three replicates. Plots consisted of three plant densities (5, 7, and 9 plants ha-1) and subplots of five N rates (0, 30, 60, 120, and 180 kg ha-1). Aboveground dry matter, leaf macro- and micronutrient concentrations, yield components, grain yield, and protein concentration in grains were evaluated. Lower plant densities (5 and 7 plants m-1) increased aboveground dry matter production and the number of pods per plant and did not reduce grain yield. In the absence of N fertilization, reduction of plant density decreased N concentration in common bean leaves. Nitrogen fertilization linearly increased dry matter and leaf N concentration, mainly at lower plant densities. Regardless of plant density, the N supply linearly increased grain yield of cultivars IPR 139 and Pérola by 17.3 and 52.2%, respectively.

RESUMO O objetivo do trabalho foi avaliar o efeito da densidade de plantas na fileira e de doses de nitrogênio (N) em cobertura na nutrição e desempenho produtivo das cultivares de feijão comum IPR 139 e Pérola. Para cada cultivar, o delineamento experimental utilizado foi em blocos ao acaso, em parcelas subdivididas e três repetições. As parcelas consistiram de três densidades de plantas (5, 7 e 9 plantas m-1 de fileira) e as subparcelas de cinco doses de N (0, 30, 60, 120 e 180 kg ha -1). Foram avaliadas: matéria seca da parte aérea, teores de macro e micronutrientes na folha diagnose, componentes da produção, produtividade de grãos e teor de proteína nos grãos. Menores densidades de plantas (5 e 7 plantas m-1) proporcionaram maior produção de MS e número de vagens por planta e não reduziram a produtividade de grãos. Na ausência da adubação nitrogenada, a redução da densidade de plantas diminuiu o teor de N nas folhas do feijoeiro. A adubação nitrogenada aumentou linearmente a produção de matéria seca e o teor de N nas folhas do feijoeiro, principalmente sob as menores densidades de plantas. Independentemente da densidade de plantas, o fornecimento de N aumentou linearmente a produtividade de grãos das cultivares IPR 139 e Pérola em 17,3 e 52,2%, respectivamente. | ABSTRACT The objective of this work was to evaluate the effect of plant densities and sidedressed nitrogen (N) rates on nutrition and productive performance of the common bean cultivars IPR 139 and Pérola. For each cultivar, a randomized complete block experimental design was used in a split-plot arrangement, with three replicates. Plots consisted of three plant densities (5, 7, and 9 plants ha-1) and subplots of five N rates (0, 30, 60, 120, and 180 kg ha-1). Aboveground dry matter, leaf macro- and micronutrient concentrations, yield components, grain yield, and protein concentration in grains were evaluated. Lower plant densities (5 and 7 plants m-1) increased aboveground dry matter production and the number of pods per plant and did not reduce grain yield. In the absence of N fertilization, reduction of plant density decreased N concentration in common bean leaves. Nitrogen fertilization linearly increased dry matter and leaf N concentration, mainly at lower plant densities. Regardless of plant density, the N supply linearly increased grain yield of cultivars IPR 139 and Pérola by 17.3 and 52.2%, respectively.