Protozoan parasites can survive under ambient and refrigerated storage conditions when associated with a range of substrates. Consequently, various treatments have been used to inactivate protozoan parasites (Giardia, Cryptosporidium, and Cyclospora) in food, water, and environmental systems. Physical treatments that affect survival or removal of protozoan parasites include freezing, heating, filtration, sedimentation, UV light, irradiation, high pressure, and ultrasound. Ozone is a more effective chemical disinfectant than chlorine or chlorine dioxide for inactivation of protozoan parasites in water systems. However, sequential inactivation treatments can optimize existing treatments through synergistic effects. Careful selection of methods to evaluate inactivation treatments is needed because many studies that have employed vital dye stains and in vitro excystation have produced underestimations of the effectiveness of these treatments.

The food processing industry is one of the largest consumers of energy and water in the manufacturing sector. It is vital that conservation measures are taken to reduce the use of electricity, fuel, and water for producers to have long-term, sustainable growth. The Pacific Northwest (PNW) region includes some the largest food processers in the United States, particularly with products such as fruit and vegetable preserves, apples products, potato products, and milk. Energy and water consumption in PNW food processing facilities are quantified as well as techniques to increase efficiency and reduce waste. Mechanical drive systems and refrigeration consumes the most electricity in the industry and the implementation of energy management plans has the largest potential to save electricity in PNW facilities. Heating and cooling process needs are the largest consumers of energy in the food processing industry. Implementing cogeneration/trigeneration technology, replacing of older equipment, capturing waste heat, and reusing wastewater can have significant impacts on both energy and water consumption. Novel, emerging technologies such as membrane separation, high-pressure processing, microwave assist, ultrasound, pulsed high electric fields, ozone, and hydrogen/electricity generation have significant potential to benefit the food processing industry by increasing efficiency and allowing companies to stay competitive in an industry where sustainable practices are becoming increasingly important to the public.

A bibliographic search yielded a set of empirical equations that constitute an easy method for the calculation of some thermophysical properties of both liquid water and ice I, properties that are involved in the modeling of thermal processes in the high-pressure domain, as required in the design of new high-pressure food processes. These properties, closely interrelated in their physical derivation and experimental measurement, are specific volume, specific isobaric heat capacity, thermal expansion coefficient, and isothermal compressibility coefficient. Where no single equation was found, an alternative method for calculation is proposed. Keeping in mind the intended applications and considering the availability of both experimental data and empirical equations, the limits for the set of equations where set in -40 to 120 degrees C and 0 to 500 MPa for liquid water and -30 to 0 degrees C and 0 to 210 MPa for ice I. The equations and methods selected for each property are described and their results analyzed. Their good agreement with many existing experimental data is discussed. In addition, the routines implemented for the calculation of these properties after the described equations are made available in the public domain.