We manipulated the amount of food and water (soil moisture) available to natural populations of larval tiger beetles in Arizona. Supplemental food increased survivorship of first instars to the second instar in all species in at least one of two years. Supplemental soil moisture usually increased, but sometimes decreased, survival of first instars. Interaction of food and water treatments was significant in a few cases, but the nature of the interaction varied greatly among species and years. Significant mortality of first instars from natural enemies occurred, and may explain the anomalous cases of first—instar survival with food exclusion (since enemies were also excluded by this treatment). Food supplementation increased first—instar—to—adult survivorship for all species and third—instar—to—adult survivorship for most species; in contrast, water supplementation had no effect on most species. Food manipulation, but not water manipulation, also affected development time or size of adults (usually both) for all seven species tested. Supplemental food produced larger adults and resulted in more rapid maturation. Reduced food resulted in delayed development, and this usually resulted in death. The results of this study provide one of the few cases in which simultaneous effects of two major ecological factors have been analyzed for a large set of similar species in an array of habitats. The prevalence of food limitation and the frequent lack of a detectable consistent interaction between food availability and water availability in this system indicate that food limitation and resource competition may be the dominant factors controlling these organisms.

The Pacific lamprey is an iconic native fish of great importance to the ecosystem and indigenous cultures in the U.S. Pacific Northwest. Pacific lamprey populations have declined in abundance from historic levels, and conservation aquaculture has been proposed as a technique to restore these populations. The present research focused on expanding the culture methods for larval lamprey. The larvae filter feed and cohabitate with juvenile salmonids in the wild, therefore the effluent water from rearing salmonids may be a viable source of water and food for culturing lamprey. This approach could be a sustainable method for raising lamprey at existing salmonid hatcheries. A nine week trial investigated the effects of rearing in effluent water from salmonid culture on the growth, survival, proximate composition, and fatty acid profile of larval lamprey. This trial also explored the potential of this rearing strategy to improve the water quality by removing nutrients from the effluent. The trial included three treatments testing the use of the effluent from steelhead trout (Oncorhynchus mykiss), a conventional lamprey diet (control diet), or both combined as a means for rearing lamprey. A fourth treatment where lamprey were excluded served as a water quality control to test the effect of lamprey presence on effluent quality. Lamprey survival was not affected by treatment. Lamprey reared solely on the effluent waste matched the survival and growth of fish fed the conventional diet. Lamprey fed the combination treatment grew faster than the conventional diet fed fish. Whole body lipid levels were elevated in lamprey from the combination treatment relative to the conventional diet fed lamprey. Crude protein in the whole bodies of effluent fed lamprey was low compared to fish from either of the treatments where the conventional diet was fed. Lamprey offered the effluent nutrients were high in saturated fats relative to the control fed fish, which reflected the lipid profile of this diet. However, lamprey from the combination treatment were lower in long chain polyunsaturated fatty acids relative to the control or the effluent treatment lamprey. There was no measureable improvement in water quality due to the presence of lamprey, though lamprey were able to sequester approximately 1.3% of the effluent dry matter as lamprey biomass. Overall, it appears the larval stage of lamprey can be effectively reared on salmonid effluent, and this method provides superior growth when used in combination with a conventional lamprey diet.

Pesticides are increasingly recognized as relevant stressors in stream ecosystems. Stream biota is exposed to pesticides with low water solubility, e.g. pyrethroid insecticides, via water, habitat, and food. However, long-term effects of simultaneous exposure pathways are unknown. In this context, we conducted a microcosm experiment with the caddisfly Anabolia nervosa exposing the larvae to the pyrethroid insecticide esfenvalerate (EFV) at 0.1 and 1.0μgL−1 via (i) water, (ii) food or a (iii) combination of water and food. Combined exposure through water and food significantly reduced emergence by 60% and significantly postponed emergence timing at the highest EFV level, whereas none of the single-phase exposures showed significant effects. Moreover, our study revealed that successfully emerged females from the highest biphasic treatment level were characterised by altered composition of storage lipids indicative of reduced energy reserves. Consequently, a realistic test scenario that represents simultaneous exposure of organisms and their food may reveal substantially increased long term effects of pyrethroids when compared with current ecological risk assessment applying only single phase exposure. We recommend that relevant concurrent exposure routes of pesticides should be considered in order to derive realistic regulatory acceptable concentrations of pesticides.

In a factorial experiment, fifth-instar Locusta migratoria (L.) (Orthoptera: Acrididae) were given either dry food (lyophilized grass) and drinking water, food only, water only, or neither food nor water. Food consumption and insect weight were measured daily, and the behaviour of each locust was recorded for 5 h on each of four consecutive days and for 2.5 h on the fifth. Consumption declined progressively in locusts given food only, and those given water only were not observed to drink after the first day of food deprivation. The decline in food consumption on the first day was accounted for by a decrease in the average duration of feeds, which remained constant thereafter. The further decline in consumption over subsequent days was due to a progressive decline in the number of feeds. Although food availability did not slow weight loss relative to locusts given neither food nor water, the availability of water without food did. The proportion of time locomoting increased in all deprivation treatments, but the pattern of change across the five observation days differed markedly between treatments. Locusts given food but no water increased locomotion from 20% of the time budget (the value for controls) to 30% on the first day of deprivation, and by the second day had reached a plateau of approximately 65%, which was maintained until the experiment was terminated on day 5. In contrast, locusts given water but no food approached the 65% level of locomotion on the first day, which was statistically greater than the 55% observed in those deprived of both food and water. This increase was due both to an increase in the number of locomotion bouts initiated and an increase in the average duration of locomotion bouts. On the second and third days, all deprivation treatments maintained locomotion at around 65%. By day 4, locomotion had decreased to approximately 15% in locusts deprived of both food and water, but not in those deprived of food only or water only. Unlike those given only food, locusts given only water showed a reduction in locomotion of c. 15% on the fifth day.

In winter 2004/2005, 1532 Water Pipits were recorded during 37 censuses carried out along an established route on a sewage farm flooded with wastewater (Wrocław, SW Poland). Single birds were seen in nearly 39% of all 299 encounters, while the largest concentrations, between 16–28 individuals, accounted for 9%. 78% of all birds were observed on meadows flooded with communal wastewater. The remaining ones stayed around irrigation ditches (n = 172, 11.5%), sedimentation basins (n = 88, 5.9%) and reedbeds (n = 72, 4.8%). The mean size of the Water Pipit concentration was largest on the meadows (mean ± SE = 6.54 ± 0.50 individuals) and smallest at the sedimentation basins (mean = 1.44 ± 0.14). In this winter season (December-first half of March), rainfall enlarged numbers of birds to forage on the meadows, and the thickness of the snow cover was positively correlated with bird abundance at the sedimentation basins. The dominant available prey items inhabiting the warm wastewater were Diptera larvae (96%), 88% of which belonged to the genus Eristalis. The mean (± SD) invertebrate biomass was highest in the basin sediments (1.03 ± 1.14 g/dm³ of deposits), and lowest on the flooded meadows (0.20 ± 0.37 g/dm³ of deposits). The results point to the significance of the artificial environmental conditions created by warm sewage water, which enable the birds to remain largely independent of the weather and thus to overwinter in a cold region of central Europe.

Females of the European beewolf, Philanthus triangulum F. (Hymenoptera, Crabronidae), embalm the provisions of their larvae, paralyzed honeybees, with a secretion from a postpharyngeal gland prior to oviposition. This food wrapping is known to delay fungus infestation of the prey. In the present study, the hypothesis that the food wrapping has an additional function, namely the prevention of prey desiccation, is tested. Water loss of paralyzed but unembalmed honeybees and embalmed honeybees is measured and the composition and quantity of their cuticular hydrocarbons analyzed by coupled gas chromatography-mass spectrometry. Water loss is significantly lower in embalmed compared with unembalmed bees. This might have important advantages for the larvae under the warm and dry conditions that prevail in some brood cells. The embalming by beewolf females increases the total amount of hydrocarbons on the surface of the bees by almost ten-fold. Moreover, the proportion of unsaturated and short-chained hydrocarbons is significantly increased. Unsaturated and short-chain hydrocarbons are usually less effective against water loss, so the increased protection against water loss appears to be mediated mainly by the thickness of the hydrocarbon layer.

The effect of fluoride on the food and dietary water intake and utilisation were studied in final instars of silkworm, Bombyx mori L. race NB₄D₂ fed ad libitum with mulberry leaves treated with sodium fluoride at concentrations of 25 ppm, 50 ppm and 75 ppm. The larval duration increased by two days while food consumption, assimilation rate, assimilation efficiency, feeding and metabolic rates increased significantly in all the treated larvae. On the other hand, the amount of faeces produced, food conversion, conversion rate and conversion efficiencies decreased significantly in all the treated batches when compared to controls. The water intake and utilisation parameters increased significantly while rate of water loss through faeces, water retained in the body and retention efficiency decreased in all the fluoride-treated silkworms. The factors responsible for these changes in treated batches are discussed in relation to the effect of fluoride on cocoon and shell weights.

Sparus aurata larvae reared under controlled water-temperature conditions during the first 24 days after hatching displayed a linear relationship between age (t) and standard length (SL): SL = 2.68 + 0.19 t (r2 = 0.91l). Increments were laid down in the sagittae with daily periodicity starting on day of hatching. Standard length (SL) and sagittae radius (OR) were correlated: SL(mm) = 2.65 + 0.012 OR(mm). The series of measurements of daily growth increment widths (DWI), food density and water temperature were analyzed by means of time series analysis. The DWI series were strongly autocorrelated, the growth on any one day was dependent upon growth on the previous day. Time series of water temperatures showed, as expected, a random pattern of variation, while food consumed daily was a function of food consumed the two previous days. The DWI series and the food density were correlated positively at lags 1 and 2. The results provided evidence of the importance of food intake upon the sagittae growth when temperature is optimal (20ºC). Sagittae growth was correlated with growth on the previous day, so this should be taken into account when fish growth is derived from sagittae growth rates.