Microbiological Examination Methods of Food and Water (2nd edition) is an illustrated laboratory manual that provides an overview of current standard microbiological culture methods for the examination of food and water, adhered to by renowned international organizations, such as ISO, AOAC, APHA, FDA and FSIS/USDA. It includes methods for the enumeration of indicator microorganisms of general contamination, indicators of hygiene and sanitary conditions, sporeforming, spoilage fungi and pathogenic bacteria. Every chapter begins with a comprehensive, in-depth and updated bibliographic reference on the microorganism(s) dealt with in that particular section of the book. The latest facts on the taxonomic position of each group, genus or species are given, as well as clear guidelines on how to deal with changes in nomenclature on the internet. All chapters provide schematic comparisons between the methods presented, highlighting the main differences and similarities. This allows the user to choose the method that best meets his/her needs. Moreover, each chapter lists validated alternative quick methods, which, though not described in the book, may and can be used for the analysis of the microorganism(s) dealt with in that particular chapter. The didactic setup and the visualization of procedures in step-by-step schemes allow the user to quickly perceive and execute the procedure intended. Support material such as drawings, procedure schemes and laboratory sheets are available for downloading and customization. This compendium will serve as an up-to-date practical companion for laboratory professionals, technicians and research scientists, instructors, teachers and food and water analysts. Alimentary engineering, chemistry, biotechnology and biology (under)graduate students specializing in food sciences will also find the book beneficial. It is furthermore suited for use as a practical/laboratory manual for graduate courses in Food Engineering and Food Microbiology.

Molecular Microbial Diagnostic Methods: Pathways to Implementation in the Food and Water Industries attempts to address the shortage of guidance on implementation of molecular-based methods for routine diagnostic laboratories. What industry and analysts can expect from routine use of these methods is discussed and outlined. The book uncovers industry needs for the use of molecular methods by providing a brief history of water and food analysis for the pathogens of concern. It also describes the potential impact of current and cutting-edge molecular methods. It discusses the advantages of the implementation of molecular methods, describes information on when and how to use specific methods, and presents why one should utilize them for pathogen detection in the routine laboratory. The reference material in the book is also pertinent for anyone carrying out microbiological analysis at the research level and covers a wide spectrum of classical and cutting-edge methods.

Information on the macronutrient composition of the diet is needed in doubly labeled water studies to convert measured rates of carbon dioxide production into values for total energy expenditure. There is no general consensus, however, about the best method to determine food intake for this purpose. Four common methods of measuring food intake (7-day weighed food intake, 24-hour recall, and Fred Hutchinson Cancer Research Center/Block and Willett food frequency questionnaires) were tested for their ability to provide comparable food quotient and total energy expenditure data in doubly labeled water studies in 10 young and 10 older women. All methods gave mean values for total energy expenditure that were within 1% of each other. Individual values obtained using the 24-hour recall and food frequency questionnaires were within +/-3% (standard deviation) of values determined using data from the 7-day weighed food record. These results suggest that it is not necessary to use time-consuming and expensive 7-day food records in doubly labeled water studies; instead, food intake data obtained more easily by 24-hour recall or food frequency questionnaire can provide comparable data.

Current studies on the food-energy-water nexus do not capture effects on human health. This study presents a new methodology for assessing the environmental sustainability in the food-energy-water-health nexus on a life cycle basis. The environmental impacts, estimated through life cycle assessment, are used to determine a total impact on the nexus by assigning each life cycle impact to one of the four nexus aspects. These are then normalised, weighted and aggregated to rank the options for each aspect and determine an overall nexus impact. The outputs of the assessment are visualised in a “nexus quadrilateral” to enable structured and transparent interpretation of results. The methodology is illustrated by considering resource recovery from household food waste within the context of a circular economy. The impact on the nexus of four treatment options is quantified: anaerobic digestion, in-vessel composting, incineration and landfilling. Anaerobic digestion is environmentally the most sustainable option with the lowest overall impact on the nexus. Incineration is the second best option but has a greater impact on the health aspect than landfilling. Landfilling has the greatest influence on the water aspect and the second highest overall impact on the nexus. In-vessel composting is the worst option overall, despite being favoured over incineration and landfilling in circular-economy waste hierarchies. This demonstrates that “circular” does not necessarily mean “environmentally sustainable.” The proposed methodology can be used to guide businesses and policy makers in interpreting a wide range of environmental impacts of products, technologies and human activities within the food-energy-water-health nexus.

The “nexus” is a potentially very appropriate approach to enhance resource efficiency and good governance in transboundary basins. Until now, however, evidence has been confined to isolated case studies and the nexus approach remains largely undefined. The methodology presented in this paper, developed for preparing a series of nexus assessments of selected river basins under the Water Convention of the United Nations Economic Commission for Europe (UNECE), is a timely contribution to this ongoing debate. The nexus assessment of a transboundary basin has the objective of identifying trade-offs and impacts across sectors and countries and to propose possible policy measures and technical actions at national and transboundary levels to reduce intersectoral tensions. This is done jointly with policy makers and local experts. Compared to an Integrated Water Resource Management approach, the water energy food ecosystems nexus approach concurrently considers multiple sectors and their evolution. This offers the opportunity to better involve key economic sectors—energy and agriculture in particular—in the dialogue over transboundary water resource uses, protection and management.

Irrigated agriculture is the backbone of Central Asian economies. Therefore, efficient irrigation water management is of crucial importance to the sustainable crop production in the region. Presented here are two studies aiming to improve agricultural water productivity â?? ET-based irrigation scheduling in Uzbekistan; and valuation of ecosystem services in Kazakhstan. The ET-based irrigation scheduling method has potential to replace subjective daily water management decisions at Water Users Association level with crop water demand-based decisions to improve water-use efficiency. Results from a two year study show that there can be a 32-35% saving of water when irrigation is applied using the ET-based scheduling method. The pilot plots are representative of 38% of irrigated area in Fergana Valley (241,407ha) and 50% in Khorezm (137,500ha) area. If this methodology is widely adopted, large amounts of water can be saved which can be diverted for other purposes. Flood irrigation of cotton is practiced on 128,000ha in the Bugunski Reservoir watershed of Kazakhstan. This practice is unsustainable due to seasonal unavailability in water supply and depletion of river discharges that were historically important at maintaining water levels downstream in nearby wetlands and the Aral Sea. Farmer surveys were used along with RIOS and SWAT modeling to evaluate alternative irrigation practices and cropping systems that can conserve water from the Bugunski Reservoir while maintaining farmer incomes. Simulations show significant reductions in irrigation water demand in the alternative scenario relative to the baseline scenario. Under baseline flood irrigation of cotton, annual irrigation demand was 928 MCM/yr averaged over the 32 year climatic record simulated. Irrigation demand decreased by 38% to 573 MCM/yr when 40,439ha of flood irrigated cotton was converted to drip irrigated cotton, sprinkler irrigated alfalfa and drip irrigated grapes. This represents a savings of 355 MCM/yr in water extracted from irrigation canals and groundwater wells | Vinay Nangia. (7/11/2016). Managing Scarce Water Resources in Irrigated Agri-Food Systems of Central Asia Two Case Studies. Phoenix, United States: American Society of Agronomy.

Contaminated water and food are major causes of malnutrition and mortality in the developing world, particularly among children. Infants are most vulnerable to diarrheal illnesses when introduced to fluids and foods as they are weaned from breastfeeding to a mixed diet. There is scant literature about the role of nutrition professionals in addressing this problem. Considerable progress has been made in identifying strategies to prevent diarrhea in children. Strategies include implementing low-technology methods of sanitizing water, emphasizing the benefits of breastfeeding, protecting prepared foods from unclean environments, and educating and motivating food preparers. Resolution of water and food safety problems requires a collaborative interdisciplinary approach among health professionals and involvement of community leaders. Dietetic professionals have the training to empower individuals and communities with skills to create a safe water and food environment.

A novel method based on ultrasound-assisted surfactant-enhanced emulsification microextraction (UASEME) has been developed for the preconcentration of cobalt prior to its determination by graphite furnace atomic absorption spectrometry. In the UASEME technique, chloroform was used as the extraction solvent, sodium dodecyl sulfate was adopted as emulsifier, and ultrasound was applied to assist emulsification. There is no need of using organic dispersive solvent which is typically required in conventional dispersive liquid–liquid microextraction method. Several parameters that affect the extraction efficiency, such as the kind and volume of the extraction solvent, the type and concentration of the surfactant, pH of sample solution, concentration of the chelating agent, and extraction time and temperature were investigated and optimized. Under the optimal conditions, the linearity of calibration curve was in the range of 0.1–5 ng mL⁻¹with a correlation coefficient (R²) of 0.9992. An enrichment factor of 58 was achieved with a sample volume of 5.0 mL. The detection limit of this method for Co was 15.6 ng L⁻¹, and the relative standard deviation (RSD) was 4.3 % at 1.0 ng mL⁻¹concentration level of Co. The accuracy of the developed method was evaluated by analysis of the certified reference materials GBW07605 tea leaf and GBW10015 spinach. The method was successfully applied to determine trace cobalt in food and water samples with satisfactory results.