Legumes, which include a great variety of seeds, are distinguished by their protein content. Legume seeds produce defensive compounds against fungi and insect predators, and these compounds can be extracted or isolated for antimicrobial use. Isolation and identification of legume proteins and peptides have been extensively studied as part of the search for antifungal compounds. Researchers have recently started to pay attention to the antimicrobial activities of legume proteins, lectins, and peptides; however, few overviews regarding their antifungal activity are available, particularly concerning food applications.This review summarizes the main legume proteins and peptides with antifungal activity and their principal antifungal mechanisms of action. Further, potential food applications of legume water extracts and legume crude protein extracts with antifungal activity are discussed.Most studies have focused on isolating and identifying proteins and peptides with antifungal activity. Antifungal mechanism of action has been established for legume defensins. In contrast, legume water extracts and legume crude protein extracts have been subjected to less investigation; however, these preparations have been explored for food applications, particularly in bread, with interesting results. Despite their antifungal activity, practical applications of legume proteins and peptides have yet to be found. This is due to their low yields, high costs, and poor safety regulatory status. Therefore, further research on legume water extracts is necessary before food applications can be broadly developed.

Pickering emulsions stabilized by food-grade particles have garnered increasing interest in recent years due to their promising applications in biorelated fields such as foods, cosmetics, and drug delivery. However, it remains a big challenge to formulate nanoscale Pickering emulsions from these edible particles. Herein we show that a new Pickering nanoemulsion that is stable, monodisperse, and controllable can be produced by employing the spherical micellar nanoparticles (EYPNs), self-assembled from the food-derived, amphiphilic egg yolk peptides, as an edible particulate emulsifier. As natural peptide-based nanoparticles, the EYPNs have a small particle size, intermediate wettability, high surface activity, and deformability at the interface, which enable the formation of stable Pickering nanodroplets with a mean dynamic light scattering diameter below 200 nm and a polydispersity index below 0.2. This nanoparticle system is versatile for different oil phases with various polarities and demonstrates the easy control of nanodroplet size through tuning the microfluidization conditions or the ratio of EYPNs to oil phase. These food-grade Pickering nanoemulsions, obtained when the internal phase is an edible vegetable oil, have superior stability during long-term storage and spray-drying based on the irreversible and compact adsorption of intact EYPNs at the nanodroplet surface. This is the first finding of a natural edible nano-Pickering emulsifier that can be used solely to make stable food Pickering nanoemulsions with the qualities of simplicity, versatility, low cost, and the possibility of controllable and mass production, which make them viable for many sustainable applications.

The particular effect of 4 kinds of amino acid and peptide-rich food material (APRM) containing different charged amino acid contents on the gelatinization and retrogradation behavior of potato starch granules and on the water-vaporization behavior was analyzed by differential scanning calorimetry, rapid viscoanalysis, x-ray diffractometry, thermal gravimetry-differential thermal analysis, and pulsed NMR. APRM with a high-charged amino acid content produced unique gelatinization and retrogradation behavior in terms of an elevated gelatinization temperature, reduced viscosity, higher setback, and lower retrograded starch melting enthalpy. The recovered x-ray diffraction intensity decreased with increasing charged amino acid content. APRM with high-charged amino acid content could provide an improved paste having easy vaporization of external water in the swollen starch granules due to the reduced swelling.

Food industry processing wastes are produced in enormous amounts every year, such wastes are usually disposed with the corresponding economical cost it implies, in the best scenario they can be used for pet food or composting. However new promising technologies and tools have been developed in the last years aimed at recovering valuable compounds from this type of materials. In particular, sub-critical water hydrolysis (SWH) has been revealed as an interesting way for recovering high added-value molecules, and its applications have been broadly referred in the bibliography. Special interest has been focused on recovering protein hydrolysates in form of peptides or amino acids, from both animal and vegetable wastes, by means of SWH. These recovered biomolecules have a capital importance in fields such as biotechnology research, nutraceuticals, and above all in food industry, where such products can be applied with very different objectives.Present work reviews the current state of art of using sub-critical water hydrolysis for protein recovering from food industry wastes. Key parameters as reaction time, temperature, amino acid degradation and kinetic constants have been discussed. Besides, the characteristics of the raw material and the type of products that can be obtained depending on the substrate have been reviewed. Finally, the application of these hydrolysates based on their functional properties and antioxidant activity is described.

Legume waster water has been revealed to contain sufficent nutritional value as an industry by-product. In this study, physicochemical properties and protein profile of pea, chickpea and soybean soaking and cooking water were investigated. Among the three legumes isolates with their wastewater, all of the cooking wastewater exhibited the lowest content of free amino acid. In the yellow pea free amino acid assay, the soaking method contains the highest volume of Ser (6.00mg/g), His (6.01 mg/g), Phe (7.48 mg/g), Ile (4.12mg/g) Thr (4.27 mg/g) and Met (1.68mg/g). Chickpea soaking water has the highest volume of His (12.05mg/g) Phe (16.43mg/g), Ile (9.77mg/g), Thr (10.12mg/g) and Met (11.08mg/g). Pea cooking water Met to Lys is 0.8, which gave it the most elastic properties to form the sponge cake and more nutritional value. To identificate the emulsifying properties, oil-in-water emulsions containing 50% Canola oil were prepared using the legumes wastewater (pea, chickpea and soybeans). These emulsions were then stored at 4°C, and changes in particle size were monitored throughout storage. Chickpea cooking emulsions showed the smallest droplets distribution in the original emulsions. The mean emulsion droplet diameter (nm) evaluated differed significantly(p<0.05) for all centrifugated legume wastewater-based emulsions. Different types of legumes affected emulsifier stability because of different protein content. In conclusion, soybean soaking and cooking water have the best emulsifier stability both at room temperature and the refrigerator temperature, pea cooking water was investigated to form the hardest sponge cake because of its highest protein content.