Oil and gas extraction in the western United States generates significant volumes of produced water (PW) that is typically injected into deep disposal wells. Recently, crop irrigation has emerged as an attractive PW reuse option, but the impact on plant immune response is not known. In this study, we conducted a 3-month greenhouse pot study. Spring wheat (Triticum aestivum) was irrigated 3 times a week with 150 mL (∼80–100% of soil water holding capacity) with one of four irrigation treatments: tap water control, 10% PW dilution, 50% PW dilution, and salt water (NaCl50) control containing the same amount of total dissolved solids as PW50 to determine the effect on disease resistance. The wheat leaves were inoculated with either bacterial or fungal pathogens and changes in pathogenesis-related PR-1 and PR-5 gene expression were measured from the leaf tissue. PW50 experienced the largest relative suppression of PR-1 and PR-5 gene expression compared to noninfected wheat, followed by PW10, NaCl50, and the tap water control. A combination of PW contaminants (boron, total petroleum hydrocarbons, and NaCl) are likely reducing PR-gene expression by reallocating metabolic resources to fight abiotic stresses, which then makes it more challenging for the plants to produce PR genes to fight pathogens. This study provides the first evidence that plant disease resistance is reduced due to irrigation with reused PW, which could have negative implications for food security.

Water resources are one of the important factors that influence regional crop production and the food security of humans. Most traditional models of crop water demand analysis are built on the basis of a certain crop or macroscopic analysis, which neglect regional crop allocation and the difference of water demand in different crop growing periods. In this paper, a new assessing model, the satisfied degree of crop water requirement, is developed to assess the impacts of water resources on production of six main food crops in China. The six main food crops are spring wheat, winter wheat, corn, early season rice, middle-season rice and late rice. The results show that: (1) there are serious risks of water shortage in China, even in south China with its abundant precipitation; (2) the satisfied degree of crop water demand represents great temporal–spatial changes. On spatial distribution the risks are high in major bases of food production due to influences of cropping system and crop-combinations. Northwest China is a special interesting case. In seasonal fluctuation water shortage is severe in March and September. These risks seriously restrict food production in China. The results also show that the strategic measures of water resources management must be chosen carefully to deal with food security and regional sustainable development in China.