Rossioglossum grande (Lindl.) Garay & G.C. Kenn is a Mexican native orchid threatened to extinguish according to the NOM-059-Semarnat-2010. In vitro asymbiotic germination is a conservation tool widely used for threatened orchids. Generate basic knowledge about in vitro response of R. grande was the object of the work, comparing seed germination in Knudson C medium (KC) versus Dalla Rosa y Laneri medium (DR), modified adding iron chelate and coconut water. We distinguished three different stages of culture: the sowing culture (CM) and two subcultivated culture (C1 and C2). Experimental design was totally aleatory. Anova, Tukey’s test (p≤ 0.05) y Chi-square were used to analyze germination variables averages. After 60 days from sowing (CM), seedling was more developed, and in general it had more percentage of green and vital protocorms using DR medium. After 12 months without subcultivate, we assist at regeneration of protocorms in DR medium in a significant way. In the subcultivation C1 and C2 we test similar responses. We assume that use of iron chelate and coconut water improves the protocorms development and their regeneration of R. grande in in vitro conditions. | Rossioglossum grande (Lindl.) Garay & G.C. Kenn es una orquídea nativa mexicana considerada en peli-gro de extinción por la NOM-059-Semarnat-2010. Teniendo en cuenta que la germinación asimbiótica in vitro es una herramienta estratégica para la conservación de orquídeas amenazadas, en este estudio se generó información básica mediante la comparación de la germinación de R. grande en medios Knudson C (KC) y Dalla Rosa y Laneri modificado con quelato de hierro y agua de coco (DR). Para el efecto se utili-zaron un cultivo madre (CM) y dos subcultivos (C1 y C2) y los tratamientos se dispusieron en un diseño experimental totalmente aleatorio. Los resultados se analizaron mediante análisis de varianza y comparación de medias con la prueba de Tukey (P ≤ 0.05) y Chi-cuadrada. A 60 días en el CM el medio de cultivo DR favoreció el desarrollo, la tasa de sobrevivencia y disminuyó la clorosis de los protocormos. A los 12 meses se presentó mayor desarrollo y mayor regeneración de protocormos. En los subcultivos C1 y C2 se registraron resultados similares lo que confirma que el DR acelera el desarrollo de los protocormos e incrementa su tamaño y tasa de regeneración. En síntesis, el empleo de hierro-quelato y agua de coco promueven el desarrollo in vitro de protocormos de R. grande.

Rossioglossum grande (Lindl.) Garay & G.C. Kenn is a Mexican native orchid threatened to extinguish according to the NOM-059-Semarnat-2010. in vitro asymbiotic germination is a conservation tool widely used for threatened orchids. Generate basic knowledge about in vitro response of R. grande was the object of the work, comparing seed germination in Knudson C medium (KC) versus Dalla Rosa y Laneri medium (DR), modified adding iron chelate and coconut water. We distinguished three different stages of culture: the sowing culture (CM) and two subcultivated culture (C1 and C2). Experimental design was totally aleatory. Anova, Tukey's test (p< 0.05) y Chi-square were used to analyze germination variables averages. After 60 days from sowing (CM), seedling was more developed, and in general it had more percentage of green and vital protocorms using DR medium. After 12 months without subcultivate, we assist at regeneration of protocorms in DR medium in a significant way. In the subcultivation C1 and C2 we test similar responses. We assume that use of iron chelate and coconut water improves the protocorms development and their regeneration of R. grande in in vitro conditions. | Rossioglossum grande (Lindl.) Garay & G.C. Kenn es una orquídea nativa mexicana, considerada en peligro de extinción por la NOM-059-Semarnat-2010. Teniendo en cuenta que la germinación asimbiótica in vitro es una herramienta estratégica para la conservación de orquídeas amenazadas, en este estudio se generó información básica mediante la comparación de la germinación de R. grande en medios Knudson C (KC) y Dalla Rosa y Laneri modificado con quelato de hierro y agua de coco (DR). Para el efecto se utilizaron un cultivo madre (CM) y dos subcultivos (C1 y C2) y los tratamientos se dispusieron en un diseño experimental totalmente aleatorio. Los resultados se analizaron mediante análisis de varianza y comparación de medias con la prueba de Tukey (P < 0.05) y Chi-cuadrada. A 60 días en el CM el medio de cultivo DR favoreció el desarrollo, la tasa de sobrevivencia y disminuyó la clorosis de los protocormos. A los 12 meses se presentó mayor desarrollo y mayor regeneración de protocormos. En los subcultivos C1 y C2 se registraron resultados similares lo que confirma que el DR acelera el desarrollo de los protocormos e incrementa su tamaño y tasa de regeneración. En síntesis, el empleo de hierro-quelato y agua de coco promueven el desarrollo in vitro de protocormos de R. grande.

Compulsory competition occurring in the world trade has led the enterprises to different marketing system. Marketing problems seems to be a problem in Turkey rather than agricultural production problems. In this aspect, marketing alternatives are sought. E-commerce is a system with more opportunities in agricultural marketing. Increasing the applicability of this system in Turkey will eliminate many problems associated with marketing in agriculture. With an active use of E-commerce in agricultural marketing, it provides convenience at marketing products of agro-related industries agencies and producers and all country will have a potential market position. In this study, possibilities offered by e-commerce to the agricultural sector, opportunities, threats, deficiencies and contributions to agricultural sector have been addressed. Without the limitations of place and time via the internet and computers, establishing national and international supply and demand balance of e-commerce seems to play important roles in maintaining an active and alive marketing. Furthermore, it is an important tool in reducing agricultural marketing problems.