Diagnosis of infection by geminiviruses by means of monoclonal antibodies raised against African Cassava Mosaic Virus
1994
Givord, L. | Regenmortel, M.H.V. van (Centre National de la Recherche Scientifique, Strasbourg (France). Inst. de Biochimie Moleculaire et Cellulaire) | Fargette, D. | Kounounguissa, B. | Thouvenel, J.C.
The aim of the work was to set up a detection test for Cassava mosaic virus which could be used on a large scale on the African continent. Monoclonal antibodies (AcMc) were produced against an isolate of African Cassava Mosaic Virus (ACVM), and tested for use in DAS-ELISA. Some of these AcMc allow the detection of all the different isolates of ACMV tested, and also of other geminiviruses. A DAS-ELISA test using two monoclonal antibiodies has been adapted for routine use for the detection of ACMV and Tomato Yellow Leaf Curl Virus in Senegal.
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