Immunoenzymatic analysis of zeins from normal and mutant corn Opaque-2 seeds
1995
Shupranova, L.V. | Zhmareva, E.N. | Eggi, Eh.Eh. | Sidorova, V.V. | Vinnichenko, A.N. | Gavrilyuk, I.P. | Konarev, V.G.
Using semipreparative isoelectric focusing in polyacrylamide gel in the pH range of 5-6, alpha-zein polypeptides have been isolated from total zeins of normal and mutant at Opaque-2 gene seeds of the corn A204. Rabbit antibodies against seven IEF zein components (both normal and mutant strains) have been obtained. A high degree of immunogenicity of alpha-zein polypeptides has been established. Data from immunoenzymatic analysis point to an extensive cross-reaction between alpha-zein polypeptides. Differences were found between antibody responses to total zeins in normal and mutant corn A204 seeds
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