A preliminary study on the method for in situ incubation of chitosan in the rumen of steers
1998
Sekine, J. (Tottori Univ. (Japan). Faculty of Agriculture) | Fuchigami, H. | Kamel, H.E.M. | Hai, J. | Oura, R. | Hishinuma, M.
To determine the incubation method of chitosan in the rumen of steers, two procedures for in situ incubation were investigated with the use of 4 Holstein steers with ruminal cannulae. Chitosan in a small bag was incubated in the rumen of steers given Italian ryegrass silage ad libitum for 12, 24, 48, 72 and 96 h with the random or orderly arrangement of the bag in a large bag. From the results, it was found that chitosan was not degraded in the rumen of steers, and the arrangement of small bags did not show a significant difference in the degradation of chitosan in the rumen
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