Cytological analysis of intervarietal rice hybrids (Oryza sativa L.)
2001
Khailenko, N.A. (National Centre of Biotechnology, 45 Timiryazev Str., 480090 Almaty (Kazakhstan). Inst. of Plant Physiology, Genetics and Bioengineering)
The meiosis was studied in 6 parents and 12 intervarietal F1s of rice. Processes of meiosis in cultivars in all phases was normal, conjugation of chromosomes was complete, 12 bivalents were observed at metaphase 1, A1- on 12 chrromosomes at each pole of cell. Cells of dyads and tetrads had correct form and contained one nucleus. The F1 hybrids percent of cells 1, had 1, less often 2 baculiform bivalents, changes from 0.3 up to 7.6%. In A1 cells with 1-2 lagging chromosomes were remarked, which in dyads organized micronucleus. Percent number of A1 cells and dyads with abnormalities changed, accordingly from 2.9 up to 11.5% and from 1.1 up to 14.9%. In all cells (from 0.9 up to 11.6%) with 1 lagging chromosome and asynchronous divisions were detected. The hybrid plants in F1 frequently had tetrads of incorrect form and abnormal divisions of spindle. In many cells 1-2 micronucleus was observed. F2 rice hybrids showed similar anomalies of meiosis. Abnormalities in meiosis processes were observed and at mutant rice forms. As a result of conducted researches the method of "cytological rejection" of hybrid and mutant rice plants was offered
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