Isolation and Characterization of Helicobacter pylori Urease Inhibitor from Rubus coreanus Miquel
Yang, S.W.;Ho, J.N.;Lee, Y.H.;Shin, D.H.;Hong, B.S.;Cho, H.Y.(Korea University, Seoul, Republic of Korea)E-mail:hycho@korea.ac.kr
A Helicobacter pylori urease inhibitor from Rubus coreanus Miquel has been isolated and partially characterized for aiming to prevent H. pylori growth and decrease harmful accumulation of ammonia in human gastric mucosa. We screened urease inhibitory activities in 519 extracts library prepared by solvent extractioin from 173 kinds of edible plants, medicinal herbs, herbs and seaweeds using a colorimetric urease assay system. As results of primary and secondary screening, 70% acetone extract of Rubus coreanus Miquel was selected as potent candidate, showing about 24% inhibitory activity. The acetone extract was sequentially partitioned into RCE/RCWI and RCB/RCW2 layers with ethyl acetate and butanol.
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