Comparison of alpha-galactosidase activity in cells and cultivation medium of lemon balm (Melissa officinalis L.)
2004
Vargova, E.,Slovenska polnohospodarska univ. v Nitre (Slovak Republic) | Korenova, M.,Univerzita Komenskeho, Bratislava (Slovak Republic) | Stano, J.
A rapid, simple and reproducible method for the identification of extracelullar alpha-galactosidase has been developed. The alpha-galactosidase secreted by callus culture or by roots of seedlings hydrolyses the substrate (1-naphthyl-alpha-D-galactopyranoside) in alpha-D-galactose and 1-naphthol, the latter forming slightly soluble reddish brown or reddish violet azo-dye after simultaneous azocoupling with hexazonium p-rosaniline or Fast Blue B. Colour changes under callus culture and around it as well as those in seedling roots are a demonstration of the activity of extracellular alpha-galactosidase by the subjects under study. The intensity of colouring is a measure of their enzymatic activity.
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