Purification and characterization of C. maculatus alpha-amylase | การศึกษาคุณลักษณะของเอนไซม์อะไมเลสจากด้วงถั่วเขียว
2008
Anussorn Wisessing(Kasetsart University. Bangkhen Campus, Bangkok (Thailand). Faculty of Science. Department of Biochemistry) | Arunee Engkagul(Kasetsart University. Bangkhen Campus, Bangkok (Thailand). Faculty of Science. Department of Biochemistry) | Arunee Wongpiyasatid(Kasetsart University. Bangkhen Campus, Bangkok (Thailand). Faculty of Science. Department of Applied Radiation and Isotopes) | Kiattawee Chuwongkomon(Kasetsart University. Bangkhen Campus, Bangkok (Thailand). Faculty of Science. Department of Biochemistry) E-mail:[email protected]
Callosobruchus maculatus causes damaging of storage mungbean seeds. This insect feeds on grain product during larval life and depends on its alpha-amylase for survival. Understanding the function of alpha-amylase is essential for a developing method for the insect control. In this studies, C. maculates alpha-amylase was purified and characterized. The alpha-amylase was purified by an affinity chromatography on a selfcoupled beta-cyclodextrin sepharose 6B. The amylase of C. maculatus has one isoform with a molecular weight of 50 kDa. Its specific activity was182.78 U/mg. The optimum temperature of alpha-amylase activity was in the range 50-60 deg C. The pH optimum for alpha-amylase was in the range 5-6.
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