Trypsin inhibitor from Canada goldenrod (Solidago canadensis): activity and physical and chemical properties | Ингибитор трипсина из золотарника канадского (Solidago Canadensis): активность и физико-химические свойства
2012
Ivanov, O.A. | Domash, V.I., National Academy of Sciences, Minsk (Belarus). The V.F. Kuprevich Institute of Experimental Botany | Gvozdeva, E.L. | Ievleva, E.V. | Valueva, T.A., The A.N. Bach Institute of Biochemistry, Moscow (Russia)
The article presents the results of isolation of protein inhibitor of trypsin (ScTI) from Solidago Canadensis rootstock by ammonium sulphate precipitation, ion-exchange chromatography and dual gel-chromatography. SDS-PAGE of ScTI revealed that it is molecular weight is being 17,5 kDa. By MALDY mass spectrometry Mr of inhibitor yielded a single peak with a Mr of 15,9 kDa. ScTI inhibited both trypsin and chymotrypsin in the stoichiometric ratio of 1:1, but did not inhibit subtilisin and thrombin. Inhibitory activity of ScTI remained unchanged over a wide range of PH, but lost at temperature above 50 Celsius degree and in the presence of beta-mercaptoethanol.
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