Research on induction and regeneration of callus initiated from seedling-derived young leaf segments of maize | 源于玉米幼苗的幼嫩叶段愈伤诱导及其植株再生的研究
2012
Chen Jing, Shandong Agricultural University, Taian (China), State Key Laboratory of Crop Biology | Sun Quanxi, Shandong Agricultural University, Taian (China), State Key Laboratory of Crop Biology | Ma Haizhen, Shandong Agricultural University, Taian (China), State Key Laboratory of Crop Biology
中国人. 以6个不同基因型的玉米自交系幼嫩叶段为外植体，研究其愈伤诱导、继代和分化过程。结果表明，3mg/L 2,4-D是诱导叶段愈伤组织较为适宜的，玉米愈伤组织的诱导及分化与基因型有密切关系。从供试的6个基因型中筛选出出愈率较高、愈伤组织形态较好的3个基因型齐319、Mo17、鲁原92，对它们的愈伤组织进行GUS瞬时表达检测表明，来源于幼苗叶段的愈伤组织GUS＋转化率较高。
显示更多 [+] 显示较少 [-]英语. The initiation, maintenance and differentiation of callus from seedling-derived leaf segments of six maize genotypes had been studied. It was revealed that the optimum concentration of 2, 4-D for the leaf segments-induced callus was 3 mg/L. Callus induction and regeneration were dependent on the genotypes. Due to their better performance in callus induction and morphology, embryogenic callus of Qi319, Mo17 and Luyuan 92 were transformed with a GUS construct. The transient expression of GUS was performed by the histochemical staining assay. The results showed that the GUS＋ frequency of young leaf segments-derived callus was high. This study provides technological reference for selection of leaf segment as an efficient explant for maize regeneration and genetic transformation.
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