Создание растений картофеля с генами для ингибирования синтеза фосфолипазы C | Creation of potato plants with genes for inhibition of phospholipase C synthesis
2012
Pundik, A.N. | Gapeeva, T.A., [email protected] | Vutto,N.L. | Tretyakova, T.G. | Volotovski,I.D., National Academy of Sciences, Minsk (Belarus). Institute of Biophysics and Cell Engineering
The binary vector system with the cassette for the expression in plants of the antisense RNA of tobacco phospholipase C gene was constructed. The transgenic plants of potato (Solanum tuberosum) of Belarussian variety Vetraz were obtained. The plants created are meant for the investigation of the role of hospholipase C in the mechanisms of adaptation of potato plants to environmental conditions. On the basis of the vector and the agrobacterial strain pBI121deltaGUS GV2260 there was received a vector system for agrobacterial transformation of plants carrying antisense constructs for inhibiting gene phospholipase C. There were selected lines of transgenic potato plants using a selectable marker: for the resistance to kanamycin and by RNA-PCR with primers for the neomycin phosphotransferase gene. Presence of the target gene for the synthesis of antisense RNA gene of phospholipase C tobacco was confirmed by DNA PCR. These lines of potato plants will be used for reception on their basis of transgenic lines with a reduced content of phospholipase C, which can be used as models for studying mechanisms of plant adaptation to environmental conditions and mechanisms of RNA interference.
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