Production of Hypoallergenic Cocoa Beans by a Pregermination Treatment Method
2015
Handoyo, T., Plant Physiology Laboratory, Faculty of Agriculture, Jember University, Jember, Indonesia | Mitsugu, A., Department of Biological Chemistry, Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka, Japan | Kim, K.M., Division of Plant Biosciences, School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University, Daegu, Republic of Korea
Pregermination treatment of cocoa beans either with the testa, group PCB ( + T), or without the testa, group PCB ( - T), was studied here to determine whether this treatment (incubation up to 120 h at 25 Celsius) has any effect on the levels of allergenic proteins or on chemical composition. Our proximate analysis included carbohydrates, proteins, and lipids. We used western blotting to measure changes in the amounts of allergenic proteins in the cocoa beans during the pregermination treatment. The protein and carbohydrate content of both groups (with or without the testa) decreased slightly during this treatment, whereas lipid content increased. Group PCB ( - T) showed more rapid metabolic processes than did group PCB ( + T) during the pregermination treatment. Western blot analysis showed that the cocoa beans contained an allergenic protein of ~28 kDa. Removal of the testa strongly reduced the amount of this allergenic protein after 72 h of the pregermination treatment. Generally, the pregermination treatment increased antioxidant activity in both groups. Significant differences in the antioxidant activity were observed between groups PCB ( - T) and PCB ( + T). Particularly, group PCB ( - T) showed high antioxidant activity at 72 h of the pregermination treatment. Thus, the combination of cocoa beans without the testa and pregermination treatment (72 h) seems to be the optimal method for production of hypoallergenic cocoa beans rich in antioxidants for patients with allergic disorders.
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