Functional characterization and comparative proteomic analysis of Arabidopsis thaliana prolyl-4-hydroxylazes knockout mutants [Thesis (M.Sc.)]
2012
Najjar Zein R.
英语. Prolyl 4-hydroxylases (P4H
显示更多 [+] 显示较少 [-]p4h9-1 and p4h11-1 using LM14 and MAC207 primary antibodies. There was similar expression of (LM14/MAC207) -bound AGP(s) in the wild type and P4H11, and P4H9 deficient lines. The use of ethanol and ß-Yariv reagent to precipitate AGPs was also involved to determine their expression. This proteomic investigation possibly reveals the involvement of other AtP4Hs than AtP4H11 that determine the expression levels of Arabinogalactan proteins.
显示更多 [+] 显示较少 [-]E.C, 1.14.11.2) are enzymes that catalyze the formation of 4-hydroxyproline residues in glycoproteins resulting in hydroxyproline-rich glycoproteins (HRGPs) which are the major proteinaceous components in the extracellular matrix in plants and they are divided into several subgroups including Arabinogalactan proteins (AGPs). They play a crucial role in plant growth and development as biological regulatory molecules. In this work, we studied the functional characterization of Atp4h11-1 T-DNA knockout mutant and the complemented mutant which should recover the wild type phenotype. The expression levels of AGPs were determined for two different mutants
显示更多 [+] 显示较少 [-]