Morphological and Transcriptional Responses to CRISPRi Knockdown of Essential Genes in Escherichia coli

Melanie R. Silvis; Manohary Rajendram; Handuo Shi; Hendrik Osadnik; Andrew N. Gray; Spencer Cesar; Jason M. Peters; Cameron C. Hearne; Parth Kumar; Horia Todor; Kerwyn Casey Huang; Carol A. Gross

Morphological and Transcriptional Responses to CRISPRi Knockdown of Essential Genes in Escherichia coli

2021

Essential genes make up only ∼5 to 10% of the genetic complement in most organisms but occupy much of their protein synthesis and account for almost all antibiotic targets. Despite the importance of essential genes, their intractability has, until recently, hampered efforts to study them.

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