Morphological and Transcriptional Responses to CRISPRi Knockdown of Essential Genes in Escherichia coli
2021
Melanie R. Silvis | Manohary Rajendram | Handuo Shi | Hendrik Osadnik | Andrew N. Gray | Spencer Cesar | Jason M. Peters | Cameron C. Hearne | Parth Kumar | Horia Todor | Kerwyn Casey Huang | Carol A. Gross
Essential genes make up only ∼5 to 10% of the genetic complement in most organisms but occupy much of their protein synthesis and account for almost all antibiotic targets. Despite the importance of essential genes, their intractability has, until recently, hampered efforts to study them.
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