DNA binding and bending by a chloroplast-encoded HU-like protein overexpressed in Escherichia coli
1997
Wu, H. | Liu, X.Q.
The Guillardia theta chloroplast hlpA gene encodes a protein resembling bacterial histone-like protein HU. This gene was cloned and overexpressed in Escherichia coli cells, and the resulting protein product, HlpA, was purified and characterized in vitro. In addition to exhibiting a general DNA-binding activity, the chloroplast HlpA protein also strongly facilitated cyclization of a short DNA fragment in the presence of T4 DNA ligase, indicating its ability to mediate very tight DNA curvatures.
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书目信息
Plant molecular biology
卷
34
期
2
页码
339
- 343
ISSN
0167-4412
其它主题
Dna-binding proteins; Dna-binding proteins; Hlpa gene; Plant proteins; Algae and seaweeds; Molecular; Guillardia theta; Dna bending; Structural genes; Dna conformation; Dna modification
语言
英语
注释
2019-12-05
类型
Journal Article; Text
2024-02-27
MODS
