DNA binding and bending by a chloroplast-encoded HU-like protein overexpressed in Escherichia coli

Wu, H.; Liu, X.Q.

DNA binding and bending by a chloroplast-encoded HU-like protein overexpressed in Escherichia coli

1997

Wu, H. | Liu, X.Q.

The Guillardia theta chloroplast hlpA gene encodes a protein resembling bacterial histone-like protein HU. This gene was cloned and overexpressed in Escherichia coli cells, and the resulting protein product, HlpA, was purified and characterized in vitro. In addition to exhibiting a general DNA-binding activity, the chloroplast HlpA protein also strongly facilitated cyclization of a short DNA fragment in the presence of T4 DNA ligase, indicating its ability to mediate very tight DNA curvatures.

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Ключевые слова АГРОВОК

algae bacterial proteins biosynthesis chemistry chloroplast dna chloroplasts cloning escherichia coli eukaryota evolution gene expression genetics stones

Библиографическая информация

Опубликовано в

Plant molecular biology

Том 34 Выпуск 2 Нумерация страниц 339 - 343 ISSN 0167-4412

Другие темы

Dna-binding proteins; Dna-binding proteins; Hlpa gene; Plant proteins; Algae and seaweeds; Molecular; Guillardia theta; Dna bending; Structural genes; Dna conformation; Dna modification

Язык

Английский

Примечание

2019-12-05

Тип

Journal Article; Text

В АГРИСе с: 2024-02-27

Формат: MODS

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