Continuous blue lighting and elevated carbon dioxide concentration rapidly increase chlorogenic acid content in young lettuce plants
2020
Shimomura, Michiru | Yoshida, Hideo | Fujiuchi, Naomichi | Ariizumi, Toru | Ezura, Hiroshi | Fukuda, Naoya
Chlorogenic acid (CGA) is a strong antioxidant that potentially reduces oxidative damage in human cells. In this study, the effects of environmental factors such as photoperiod, light quality and intensity, and CO₂ concentration on the growth and CGA content of lettuce (Lactuca sativa L.) were evaluated. CGA content in fresh lettuce increased under high light intensity treatments, doubling in concentration under 200 μmol m⁻² s⁻¹ compared to 100 μmol m⁻² s⁻¹. Elevated CO₂ concentration also increased CGA content in fresh lettuce, quadrupling in concentration when grown at 1000 ppm compared to 400 ppm. Furthermore, there was a compound effect of light intensity and CO₂ concentration whereby a light intensity level of 200 μmol m⁻² s⁻¹ and CO₂ of 1000 ppm produced an even higher concentration of CGA, 199 mg per 100 g of fresh lettuce. Increased CGA concentration because of continuous lighting and elevated CO₂ was observed under both fluorescent light and blue LED, but not under red LED treatment. Increased day length also induced higher CGA content in lettuce plants. These results show that continuous lighting, including blue spectrum and elevated CO₂ concentration can cause higher CGA accumulation in lettuce plants. The observed increase in CGA content was induced only for 2 days after treatment was initiated. One possible interpretation of the data is that physiological stress caused by excess photosynthesis under continuous lighting results in higher CGA content to protect the plant body from high levels of reactive oxidative species. In addition, blue light and CO₂ could be stimulus signals for inducing high CGA accumulation via metabolite changes.
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