Single-Drop and Nanogram Level Determination of Sulfite (SO32-) in Alcoholic and Nonalcoholic Beverage Samples Based on Diffuse Reflectance Fourier Transform Infrared Spectroscopic (DRS-FTIR) Analysis on KBr Matrix
2007
Verma, S.K. | Deb, M.K.
The diffuse reflectance Fourier transform spectroscopic (DRS-FTIR) method, using potassium bromide matrix, has been developed for the one-drop microdetermination of sulfite in beverage samples. The present method is very simple, rapid, and precise for the determination of sulfite. The nanogram level of sulfite determination is based on the selection of a quantitative analytical peak at 495 cm-1 among the three observed vibrational peaks obtained by diffuse reflectance Fourier transform infrared spectroscopy (DRS-FTIR). As little as a single drop of sample is sufficient for quantitative analysis of sulfite. The limit of detection (LOD) and the limit of quantification (LOQ) of the method are found to be 8 and 40 ng of SO32- 0.1 g-1 of KBr matrix, respectively. The linear range of the method (LR) as well as the LOD based on the concentration of sulfite in the solution are 5-500 and 0.8 μg/mL, respectively. The precision in terms of standard deviation and relative standard deviation value at a level of 100 ng of SO32- 0.1 g-1 of KBr for n = 10 are found to be 2 ng of SO32- and 2.3%, respectively. The relative standard deviation (n = 10) for the determination of sulfite in beverage samples available in the local market was observed to be in the range of 2.4-7.8%. The method is free from interionic effects of foreign species. No sample pretreatment is required in this method. The proposed method avoids the requirement of large numbers and bulk amounts of reagents. The method is well-suited to the need of green chemistry.
显示更多 [+] 显示较少 [-]