Efecto de la adición de aceites vegetales al sustrato de fermentación sobre las comunidades de bacterias ruminales en cultivos continuos (Rusitec) | Effects of the addition of vegetable oils to feed on rumen bacteria communities in continuous cultures (Rusitec)
2009
Vargas, J. E. | Andrés, Sonia | Yáñez Ruiz, David R. | López, Secundino
3 páginas, 1 tabla.-- Trabajo presentado a las XIII Jornadas de Producción Animal AIDA (Zaragoza, 12 y 13 de mayo, 2009)
显示更多 [+] 显示较少 [-]The objective of this work was to investigate the effects of the addition of vegetable oils to the feed on ruminal bacteria communities in continuous cultures of mixed ruminal microorganisms. Rusitec vessels were fed a total mixed ration, without oil supplement (control) or with the addition of one vegetable oil (olive, sunflower or linseed oils). Fermentation was maintained in the cultures for three weeks, samples of digesta were collected and bacterial DNA was extracted for studies of microbial diversity using the T-RFLP method. Experimental treatments were significantly clustered from the T-RFLP profiles, so that unsupplemented cultures were clearly differentiated. Using fragment size libraries for a most probable identification of peaks recorded differences between control and supplemented cultures could be established. It was concluded that oil supplementation had a significant and selective effect on ruminal bacteria communities.
显示更多 [+] 显示较少 [-]Investigación financiada por la CICYT AGL2005-04760-C02-02 y la Junta de Castilla y León (GR158 y LE007A07)
显示更多 [+] 显示较少 [-]Peer reviewed
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