The materials used in this study were four commercial varieties of Egyptian cotton; two (Giza 86 and Giza 90) belonging to the long staple class and the others (Giza 88 and Giza 92) belonging to the extra-long staple category. Within each variety, nine lint cotton grades namely: Fully Good (FG), Good/Fully Good (G/FG), Good (G), Fully Good Fair/Good (FGF/G), Fully Good Fair (FGF), Good Fair/ Fully Good Fair (GF/FGF), Good Fair (GF), Fully Fair/Good Fair (FF/GF) and Fully Fair (FF) were used. Thus Fully Good (FG) is the top quality grade and the others are progressively lower; i.e. Fully Fair (FF) is the lowest grade. Fiber properties were measured by using the Cotton Classifying System Version-5 instrument (CCS-V5). Data collected for the following characteristics were: reflectance degree (Rd %), yellowness degree (+b), trash%, dust%, fiber fragments%, total trash%, No. of neps and Micronaire value. Mean squares due to all nine grades of long and extra-long staple cotton varieties in combined analysis were highly significant over seasons for all studied characters except yellowness (+b) and fiber fragments% in Giza 92. Highly significant mean performance values of four cotton varieties and nine lint grades for each variety for all studied characters, it became clear that there were significant genetically differences between studied varieties and fiber cotton lint grades within each variety. Gradually increased for yellowness degree, trash%, dust%, fiber fragments%, total trash% (trash, dust, fiber fragments) and number of neps, while gradually decreased for reflectance degree (Rd%) and micronaire value with significant level as transfer for all tested varieties from (FG) grade down to the (FF). All studied varieties showed highly significant negative correlation between fiber cotton grade with yellowness, trash, dust, total trash% and No. of neps, while highly significant positive correlation between fiber cotton grade with reflectance degree and micronaire value. Total trash% and micronaire value as well as reflectance degree and their interactions were the most contributing and influencing of fiber cotton grades. Also these properties and their joint effects are prevailing factors which affect the personal judgment of the grader at evaluating the cotton grade.

The development of agriculture over the last decades has resulted in a continuous change of the natural vegetation of Egypt. Several species widely cultivated as economic crops or ornamentals. Numbers of species which escaped from cultivation and naturalized in naturals habitats are increasing. It is necessary to study the changes in the natural vegetation and traces the new species. The present study undertakes a survey, taxonomical revision and numerical analysis of the genus Hibiscus L. in Egypt including wild and cultivated species. The taxonomic treatment based on collecting of fresh materials from the studied species, in addition to the investigation of the herbarium specimens as well as information from the literatures. Eleven species of Hibiscus were reported in this study, from which there are 5 wild species (H. diversifolius Jacq., H. micranthus L. f., H. vitifolius L., H. sabdariffa L. and H. trionum L.) and there are 6 cultivated species (H. cannabinus L., H. mutabilis L., H. rosa-sinensis L., H. schizopetalus (Mast.) Hook. f. in Curtis, H. syriacus L. and H. tiliaceus L.). An identification key to the species of Hibiscus is provided. Valid names and synonyms are updated from online sources. Scientific descriptions provided the characters of leaves, flowers, fruits and seeds based on the herbarium specimens and completed from fresh materials and literatures are given. Distribution of wild species in their phytogeographical regions are provided. Type specimen, habitat, selected specimens and economic importance are provided for each species. The numerical analysis based on thirty six morphological characters including vegetative, flowers and fruits parts of the studied species. SPSS Statistics version 22 used to get morphometric analysis. The numerical analysis revealed two main clusters. The first cluster included 3 species, viz. H. micranthus L. f., H. vitifolius L. and H. trionum L. The second cluster included two groups: group (a) viz. H. diversifolius Jacq., H. syriacus L., and H. tiliaceus L., and group (b) viz. Hibiscus cannabinus L., H. mutabilis L., H. rosa-sinensis L., H. sabdariffa L. and H. schizopetalus (Mast.) Hook. f. in Curtis.

Six local Egyptian commercial cultivars of family Lamiaceae, two cultivars of genus Ocimum L. (Basil), two cultivars of genus Menthe L. (Mint), and two cultivars of genus Thymus L. (Thyme) were analyzed for ribulose 1,5-bisphosphate carboxylase Large (rbcL) gene at the level of DNA sequences. All samples successfully amplified the ± 630 bp fragment. Additionally, the results of alignment analysis using BLASTN tools divulged that the sequence of DNA rbcL for the two local basil cultivars (Basil1 & Basil2) has similarities with (Ocimum basilicum, Ocimum tenuiflorum, Ocimum kilimandscharicum and Ocimum gratissimum) 100, 99.69, 99.37 and 99.06 respectively. In addition, two local mint cultivars (Mint 1 and Mint 2) has similarities with Mentha spicata, Mentha_pulegium, Mentha canadensis and Mentha menthaefolia, 99.85, 99.84, 99.69 and 99.53 respectively. For thyme local cultivars (Thyme1 and Thyme2), Thym1 cultivar sample genotype is genetic closely with species, (Thymus alsinoides and Thymus sibthorpii) with 99.69 and 99.84 respectively and they located nearest from the cluster (Thymus genus) members in phylogenetic trees while, Thym2 is located after the cluster with Artemisia genus belonging to family Asteraceae. The reason of this result may be occurring due to that a Thym2 genotype comes from local marketing, which some of them are selling it as a thymus genus however, it is belongs to Artemisia genus and has similarities with with seven species of Artemisia genus (Artemisia absinthium, Artemisia gmelinii, Artemisia selengensis, Artemisia scoparia, Artemisia maritima, Artemisia capillaris and Artemisia fukudo). Using of rbcL DNA barcode proves to be effective in identifying the plants from the family level up to the genus level. This study demonstrates the efficiency of using rbcL barcoding primer to classify family Lamiaceae phylogenetically. It is also concluded that the rbcL gene showed genuine potentials to distinguish the plant Egypt species under investigation into the proper family and genus. s to distinguish the plant Egypt species under investigation into the proper family and genus.

Key Words: Media filter, Treated wastewater, Drip irrigation, Cotton Growth and Yield.INTRODUCTIONAccording to Ministry of Water Resources and Irrigation (MWIR), Egypt (2014) Agriculture expends a large amount of the obtainable water in Egypt, with its share exceeding 85% of the total demand for water. Utilizing treated wastewater represents a viable option. The study were carried out at Sarapium Forest, Ministry of Agriculture and Land Reclamation in “Sarapium”, Ismailia Governorate, Egypt, during 2018 and 2019 seasons to investigate the effect of media depth on the performance of different types of emitters for irrgating cotton (verity Giza 94) using treated wastewater. Also this study estimates the effect of using treated wastewater on the cotton growth, quantity and quality. The first experiment design for filtration performance was a split-plot with four replications. The main plots involved two media filtration depths (50 cm and 70 cm) and the sub-plots involved the time of operation (0, 25, 50, 75 and 100h). While the second experiment design for planting cotton was a split-plot with three replications. The main plots involved two plant distribution (Mutual and Opposite) and the sub-plots involved the three types of emitters namely: online 4 l/h compensative, online 4 l/h non-compensative and built- in 4 l/h-30cm non-compensative the distance between emitters were (30 cm).The results indicated that: Increasing media filtration depth from 50 to 70 cm has led to decrease the filtration flowrate with increasing pressure losses, biological oxygen demand (BOD5) and total suspended solids (TSS). The filtration flowrate decreased by increasing operation time from 0 to 100 but pressure losses, BOD5 and TSS was increased. Emitters performance of online compensative and built-in non-compensative were generally better than the online non-compensative under using wastewater quality and emitters performance decrease by increasing operation time from zero to 100 hours. Plants distribution significantly effect on growth and yield components of cotton. Planting cotton by mutual method gave the highest values of number of opened bolls per plant, seed cotton yield (Ken./fed.)

Nanoparticles (NPs), especially from micronutrients, are recently motivated for replacing their common mineral counterparts. To evaluate their comparative efficacy, this investigation was conducted to estimate the impact of foliar application of zinc through different sources on productivity, fruit quality and improve marketable fruit of “Wonderful” pomegranate trees. The field experiment was performed during two seasons (2017 and 2018) on seven - year old pomegranate trees “Wonderful” cv., cultivated in a private “Hegazi” farm located at 57 km. from Cairo on the road to Alex., Egypt. Four sources of zinc named “Zinc Sulphate, Zinc mannitol complex, Bio-Nano zinc (Bio Zn NPs) and Zinc Oxide nanoparticle (ZnO NPs) with four rates from each other were sprayed twice (the first before one week from full bloom and the second after a month from the first). So the experiment included seventeen treatments in a sample study spread in a randomized complete block design by five replicates. Results explicated that the greatest significant values of fruit set% were recorded by Bio Nano Zinc (Bio Zn NPs) treatments especially (400 ppm Bio-Nano Zinc (Bio Zn NPs)). Spraying with (3000, 4000 ppm Zn mannitol complex) and (300, 400 ppm Bio-Nano Zinc (Bio Zn NPs)) showed significantly the greatest values of productivity, improves marketable fruits and fruit quality of “Wonderful” pomegranate trees. So it could be recommended by spraying “Wonderful” pomegranate trees by 3000 ppm Zinc mannitol complex or 300 ppm Bio-Nano zinc (Bio Zn NPs). Another important point is that the application of Bio Zn NPs fertilizer at around 10% from the commercial dose of zinc sulphate resulted in the same results without any change in the productivity, further researches are needed to study a further low level of Zinc Oxide nanoparticle (ZnO NPs) below (100 ppm Zinc Oxide nanoparticle (ZnO NPs)) which may be improving yield and fruit quality

Two field experiments were conducted to assess chicken manure (Ch.M.) and its tea on quantity and quality yield of Molokhia (Jew’s mellow) plant during two summer seasons of 2017 and 2018 in Fac. of Agric. -El-Shatby- Alexandria University. This investigate aimed to compare between chicken manure (Ch.M.R 100% and R50%) , chicken manure tea (R100% and R50%) and the mixture of them (R50% of Ch.M. + R50% of its tea) under four levels (NPK0 % , NPK30%, NPK60% and NPK100%) of chemical fertilizers. Every experiment was carried out with 13 treatments in a randomized complete block design (RCBD) with three replicates (total plot area= 2&1=2m2). The results indicated that, chicken manure tea 100% + 30% NPK(T6) gave the highest mean values of vegetative growth i.e. plant height (92.0cm), number of leaves/plant (29.5), leaf length (13.87 cm), increases in vitamin C. (110.77 mg/g F.W.) and dry weight of leaves (10.25 g) as an average of both seasons. While the mixture of “chicken manure 50% and chicken manure tea 50 %” + 30 % NPK (T10) lead to significant increases in fresh leave weight (49.84 g/plant) and total yield of fresh leaves (4.7 kg/ plot) as an average of both seasons. Highest increases in P uptake (1.37mg/g) and K uptake (20.27 mg/g) either chicken manure 100% (T1) or the mixture of “chicken manure 50% and chicken manure tea 50 %” T9, respectively. The maximum N uptake (2.79 mg/g), total chlorophyll in leaves (47.44 SPAD) and leave width (6.82 cm) as an average of both seasons by the mixture of “chicken manure 50 % and chicken manure tea 50 %” + 100 % NPK (T12) compared with the control once. Organic plants “safe food” produced with organic fertilizers and their teas really supply more nutrients and vitamins “chemical free” and health protective so used sustainably in food and environmental friendly.

Two hundred and ten unsexed broiler chicks (Arbor Acres) at 7 day of age were used to study the effect of dietary supplementation of different Copper (Cu) forms i.e. inorganic (copper sulfate, CuSO4), organic (copper methionine Cu2-Met) or nano inorganic copper particles (Cu-NP) on the productive performance, hematological and biochemical constituents of blood and immune response of broiler chicks. They were randomly divided into seven dietary treatments with five replicate cages per treatment, six chicks for each. The first group was fed the basal diet without any supplementation (control); while the 2nd and 3rd groups were fed the same diet supplemented with 50 and 100 ppm of inorganic Cu (copper sulfate, CuSO4), respectively. The 4th and 5th groups were fed the basal diet supplemented with 50 and 100 ppm of organic Cu (copper methionine Cu2-Met), respectively. The 6th and 7th groups were fed the basal diet supplemented with 50 and 100 ppb of nano inorganic copper (Cu-NP), respectively. Results showed that chicks fed the basal diet supplemented with different forms of Cu had significantly better LBW, BWG, FCR, economic efficiency and production index. Both organic and nano Cu-fed groups showed significantly better productive performance traits compared with the inorganic Cu – fed groups. Supplementation of different Cu forms decreased serum levels of total lipids, triglycerides, cholesterol, low density lipoprotein (LDL), whereas RBCs count, hemoglobin, PCV, glucose, globulin, thyroid hormones (T3-T4), immunoglobulin (IgM, IgG), and antioxidant enzymes activity (TAC– GPX- GSH-SOD) were significantly increased compared with the control treatment. Additionally, Cu supplementation increased lactobacillus sp. while decreased total bacterial count (Salmonella, E. coli and Proteus) compared with the control group. In conclusion, Cu supplementation improved the growth performance, immune response and physiological status of broiler chickens. Broilers fed organic and nano Cu-supplemented diets had better growth performance and immune response than those fed inorganic copper.

Artificial intelligent provides diverse solutions for the complex problems in agriculture research. The study aimed to use three models of artificial neural networks (Feed Forward Neural Network (FFNN), Generalized Regression Neural Network (GRNN) and Radial-Basis Neural Network (RBNN)) in the field of wheat yield prediction. 27-year data for the period (1986-2012) were utilized to improve the models and four-year data (2013 and 2016) were used to estimate the models, to compare their outputs with the measured data. Prediction data was not entered in the process of building neural network models. The results showed that the optimal configuration of the FFNN model consists of 40 neurons in the hidden layer (8-40-1). The Tan Sigmoid activation function was used in both the hidden layer and the output layer using all of these models (anterior neural feeding network and the regression neural network and radial base neural network) in the 4-year wheat yield forecast field for production (2013-2016) by applying 8 input parameters that were result of NMMS (8.6%, 7.6% and 15.7% resp.), To find that FFNN and GRNN provide the best result from BRNN because while the information set was large or in a wide range, then the range data ranges from -1 to +1 (normalization data) , GRNN gives better outcomes after the information or sample data were in large range.

Excised explants were in-vitro cultured on multiplication medium of Murashige and Skoog (MS). This study was carried away inside the tissue culture lab. Horticulture Research Institute, Agricultural Research Center. Giza, Egypt through the period from 2015 to 2017, to research some factors affecting in-vitro propagation of the indoor ornamental plant Alocasia amazonica using benzyladenine amino purine (BAP) and Kinetin at 0, 1, 2, 3, 4 ppm and their interaction. The obtained results indicated that BAP gave the greatest number of shoots, plus the lowest values for shoot length, shoot fresh weight, number regarding roots and total chlorophyll content. Meanwhile, kinetin achieved the highest values for shoot length, shoot fresh weight although it was not necessarily significant. The same was observed in number of leaves, number of roots and total chlorophyll content with no significant difference. MS medium free of hormones demonstrated the greatest number of leaves, number of roots and total chlorophyll content, and the lowest values of number of shoots and shoot length. Using cytokinn at 1 ppm gave the highest shoot length and number of leaves; and the second position for number of shoot and roots. As for 2 ppm of cytokinin application, it gave the greatest values of shoot length, number of leaves and shoot fresh weight, despite the last one was not significant. this concentration got also the other position for number of shoots, 3 ppm had the greatest number of shoots, and the lowest shoot length, number of roots and shoot fresh weight and 4 ppm occupied the second grade concerning number of shoots, and the lowest grades for shoot length, shoot fresh weight, number of leaves, number of roots and total chlorophyll. Regarding the interaction between cytokinin type and concentration found that, the control treatment (Free MS) gave the highest number of leaves. Using BAP at 2 or 3 ppm attained the highest number of shoots. Using Kin at 1 or 2 ppm attained the highest shoots length. Also, Using Kin at 2 ppm attained the highest fresh weight. The application of Kin at 1 ppm was connected with the highest value of number of leaves. The development of roots showed great values on free medium of BAP and Kin as well as medium supplemented with Kin at 1 and 2 ppm. Whereas, root did not demonstrate any presence at higher concentrations of BAP of 2, 3 and 4 ppm. It is usually recommended to use the MS medium supplemented with BAP at 3 ppm which often gave the highest number of shoots. However, the highest values for shoot length, shoot fresh weight and number of roots were recorded on particularly on MS medium supplemented with Kin at 2 ppm.

Sherbet and Sorbet mixes were prepared to contain 9% fresh mango pulp, per se, 1% cooked pumpkin (CP). The water quantity required for both was replaced with ultrafiltrated milk permeate (UFMP) at the level of 25, 50 or 75%. The obtained results indicated that, separately, CP composed of higher moisture and total nitrogen, total phenolics (TP) as well as radical scavenging activity (RSA) values and lower total flavonoids (TF), carbohydrates contents than mango pulp. In both, Sherbet and Sorbet mixes, water substitution with UFMP was associated with increase in total solids, total and reducing sugars as well as ash contents. Neither acidity %, nor pH values were significantly affected by the partial substitution of water with UFMP in Sherbet mix, although the acidity % of Sorbet mix was significantly increased by UFMP addition. Freezing point of Sherbet or Sorbet mixes was gradually minimized by increasing the substitution level, although the flow behavior index was not influenced in Sherbet but increased in Sorbet mixes. The proportional increment of UFMP level in mix was associated with gradually considerable increase in its consistency coefficient, apparent as well as dynamic viscosities, TP and RSA values of both Sorbet and Sherbet mixes. UFMP led to increase in the specific gravity of mixes before and after freezing as well as elevated the overrun and strengthened the melting resistance of the final product. Furthermore, the frozen dessert of 75% UFMP, whether being Sherbet or Sorbet gained the highest panelists scores for all sensory attributes. It could be concluded that, it could successfully produce a product that meets the intended health purposes based on the substitution of 10% of mango pulp with pumpkin solids of many impressive health benefits as well as the utilization of ultrafiltration milk permeate as a source of the essential electrolytes instead of 75% of required water en route to innovate frozen desserts such as Sherbet and Sorbet