The study was to find out the changes of hormone levels in blood serum and milk of Holstein cows during the estrous cycle. The progesterone, estradio 1-17beta from the blood serum and milk samples were assayed by radioimmunoassay methods. During 20 to 25 days after artificial insemination, the accuracy of pregnancy diagnosis from the blood serum progesterone levels were 87.5% for non pregnant cows (2.Ong/ml) and 83.3% for pregnant cows (3.Ong/ml). The accuracy of pregnancy diagnosis from the milk progesterone levels were 75.0% for non-pregnant cows (2.4ng/ml), and 94.4% for pregnant cows (3.2ng/ml)

Five Korean native goats were used to test the effectiveness of the 2 modified techniques (Method 1 and 2) of Pawlow's pouch method. Both methods turned out to be successful and simpler than the original Pawlow's pouch method. In both methods, the miniature rumens were formed on the junction of 2 veins; vena ruminalis sinistra and vena ruminalis dextra. Both method 1 and 2 have the advantage of shortening the operation time, but caution was necessary to prevent the fistual formation between the rumen and miniature rumen in Method 1.

As a fundamental study to increase the reproductive efficiency in cattle, highly motile sperm were separated and collected from raw semen, extended semen and frozen semen by different methods using various concentrations of bovine serum albumin or Tyrode's solution. Various characteristics and light microscopic and electron microscopic morphology of sperm separated by different methods were compared

The in vitro activity of penicillins (penicillin G, ampicillin, carbenicillin, methicillin and cloxacillin) and gentamicin to Staphylococcus aureus and coagulase-negative staphylococci isolated from bovine mastitic milk samples was determined. The growth of all isolates of staphylococci tested was inhibited by cloxacillin and methicillin at concentration of 0.78 micro g/ml and by gentamicin at a minimum inhibitory concentration (MIC) of 1.56 micro g/ml

An experiment was conducted to measure the cellular immune response in guinea pigs by sensitizing the animals with 2,4-dinitrochlorobenzene (DNCB). The guinea pigs could be sensitizing with one application of DNCB. The sensitizing and challenge dose was standardized. The histological response was characteristic of a delayed hypersensitivity reaction

Fecal material from cattle, which was confirmed to be infected with Johne's disease by clinical and pathological symptoms, was decontaminated with 4% NaOH and inoculated into the Lowenstein-Jensen media supplemented with 1% of heat-killed Mycobacterium bovis. After 2-4 week-incubation at 37 deg C, typical acid-fast mycobacteria was isolated. The requirements of mycobactin for growth and the other biochemical properties, isolated mycobacteria was identified as Mycobacterium paratuberculosis

The histopathological changes on the cardiac and skeletal muscles, and liver of rabbits fed commercial ration were observed. In the skeletal muscle, light microscopic changes observed were cloudy swelling, hyaline degeneration, atrophy, fragmentation and coagulative necrosis of muscle fibers, and proliferation of fibroblasts and infiltration of mononuclear cells. In the myocardium, loss of muscle fibers with necrosis and vacuolization, hyaline degeneration, hemorrhage and congestion were noted

This study was conducted to determine variations in the synthesis of DNA in the nuclei of various elements of the golden hamster (Mesocricetus auratus) placenta with increasing gestational age from the 8th day post coitum to parturition. The mitotic activities of the endodermal cells of the visceral yolk sac and of the parietal yolk sac, the trophoblastic cells of the labyrinth and the trophospongium and the giant cells were decreased with increasing gestational age. The placentation was nearly completed by 13 day of pregnancy and the increase in size of labyrinth was by appositional growth from the trophospongial cells

This study was conducted to investigate in the infection rate of common internal parasites in dogs in Kwangju area (Korea R.) from January to March, 1982. Dog's stools were collected from veterinary hospitals, houses, intensive dog-rearing places and sidewalks. On the basis of egg counting, of 464 fecal samples, 73.5% were appeared as positive. Dogs from veterinary hospitals and intensive dog-rearing places have higher infection rates than others. Of the parasites detected, Toxocara canis was the most commonly encountered (23.7%). Ascarids, flukes and coccidia were more prevalent in pups than in mature dogs, whereas hookworms were detected less often in young dogs than in older ones. There were significant (p0.01) differences among age categories for all parasites except tapeworms

This study was performed to clarify the clinical signs of macroscopical lesions of pigs naturally infected with hog cholera. There was clinical and macroscopical observation on the natural cases of hog cholera and experimental cases inoculated with ALD virus and isolated virus strains. In the clinical inspection of the natural cases, diarrhea (73.1%), blotching of ear (50.0), conjunctivitis (32.7%) and dyspnea (30.8%) were observed. Dyspnea, constipation and erythema of skin were observed mainly in the experimental cases, however, staggering and conjunctivitis in pigs infected with ALD virus were found and convulsion and hemorrhage of skin of pigs infected with isolated virus were seen, respectively. The gross lesions of natural cases were hemorrhage of lymph node (82.5%), pneumonia (55.0%), gastritis and hemorrhage (52.5%), cardial hemorrhage (40.0%) and renal petechiation (37.5%), while in the experimental cases, hemorrhage of lymph node, pneumonia, gastritis and hemorrhage, enteritis and hemorrhage of large intestine and splenic infarction were seen mainly