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A novel association of BoLA DRB3 alleles in BLV infected cattle with different proviral loads 全文
2017
Nieto Farias, María Victoria | Caffaro, María Eugenia | Lendez, Pamela Anahí | Passucci, Juan | Poli, Mario Andres | Ceriani, María Carolina | Dolcini, Guillermina Laura
A novel association of BoLA DRB3 alleles in BLV infected cattle with different proviral loads 全文
2017
Nieto Farias, María Victoria | Caffaro, María Eugenia | Lendez, Pamela Anahí | Passucci, Juan | Poli, Mario Andres | Ceriani, María Carolina | Dolcini, Guillermina Laura
Bovine leukemia virus (BLV) is associated with the most common neoplastic disease of cattle. BLV has a silent dissemination in the herd due to infected cell exchange, thus the concentration of BLV-infected cells in blood should play a major role in the success of viral transmission. Genes from Bovine leukocyte antigen (BoLA), the MHC system of cattle, are associated with genetic resistance and susceptibility to a wide range of diseases, and also with production traits. Some BoLA DRB3.2 allele polymorphisms in Holstein cattle have been associated with resistance or susceptibility to BLV-disease development, or with proviral load (PVL). This investigation studied 107 BLV-infected Argentinean Holstein dairy cows, all of them belonging to one herd. PVL was analysed by qPCR and animals were classified as high proviral load (HPVL, N = 88) and low proviral load (LPVL, N = 19), and BoLA DRB3.2 alleles were genotyped. Alleles BoLA DRB3.2*1501 and *1201 were significantly associated with HPVL (p = 0.0230 and p = 0.0111 respectively), while allele BoLA DRB3.2*0201 was significantly associated with LPVL (p = 0.0030). The present study aims at contributing to the knowledge of the association between BoLA polymorphism and development of a BLV infection profile. Genes that best explain the PVL in this population resulted BoLA DRB3.2*0201 (as a protection factor) and *1501 (as a risk factor). Allelic differences may play an important role in the development of effective immune responses. A better understanding of how BoLA polymorphism contributes to these responses and the establishment of a BLV status is desirable to schedule and evaluate control measures. | O vírus da leucemia bovina (BLV) está associado à doença neoplásica mais comum do gado bovino. O BLV tem uma disseminação silenciosa no rebanho devido à troca de células infectadas, assim, a concentração de células BLV infectadas no sangue deve desempenhar um papel importante no sucesso da transmissão viral. Os genes do antígeno leucocitário bovino (BoLA), sistema MHC do gado bovino, estão associados à resistência genética e à susceptibilidade a uma ampla gama de doenças, bem como às características da produção. Alguns polimorfismos de alelos de BoLA DRB3.2 em bovinos Holstein têm sido associados à resistência ou susceptibilidade ao desenvolvimento da doença BLV, ou com carga proviral (PVL). Esta investigação avaliou 107 vacas leiteiras da raça Holstein argentina infectadas com BLV e pertencentes a um único rebanho. A PVL foi analisada por qPCR, os animais foram classificados em alta carga proviral (HPVL, N = 88) e baixa carga proviral (LPVL, N = 19), e os alelos BoLA DRB3.2 foram genotipados. Os alelos BoLA DRB3.2*1501 e *1201 estavam significativamente relacionados à HPVL (p = 0,0230 e p = 0,0111, respectivamente), enquanto o alelo BoLA DRB3.2*0201, à LPVL (p = 0,0030). O objetivo deste estudo é contribuir para o conhecimento da associação entre o polimorfismo de BoLA e o desenvolvimento de infecção por BLV. Os genes que melhor explicam a PVL na população analisada resultaram em BoLA DRB3.2*0201 (como fator de proteção) e *1501 (como fator de risco). As diferenças alélicas podem desempenhar um papel importante no desenvolvimento de respostas imunitárias eficazes. Uma melhor compreensão de como o polimorfismo BoLA contribui para estas respostas e o estabelecimento de um estado BLV é desejável para agendar e avaliar as medidas de controle. | Inst. de Genética "Ewald A. Favret"- IGEAF | Fil: Nieto Farias, María Victoria. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Centro de Investigación Veterinaria de Tandil, Laboratorio de Virología; Argentina | Fil: Caffaro, María Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina | Fil: Lendez, Pamela Anahí. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Centro de Investigación Veterinaria de Tandil, Laboratorio de Virología; Argentina | Fil: Passucci, Juan. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Área de Epidemiología; Argentina | Fil: Poli, Mario Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina | Fil: Ceriani, María Carolina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Centro de Investigación Veterinaria de Tandil, Laboratorio de Virología; Argentina | Fil: Dolcini, Guillermina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Centro de Investigación Veterinaria de Tandil, Laboratorio de Virología; Argentina
显示更多 [+] 显示较少 [-]A novel association of BoLA DRB3 alleles in BLV infected cattle with different proviral loads 全文
2017
María Victoria Nieto Farias | María Eugenia Caffaro | Pamela Anahí Lendez | Juan Passucci | Mario Poli | María Carolina Ceriani | Guillermina Laura Dolcini
Bovine leukemia virus (BLV) is associated with the most common neoplastic disease of cattle. BLV has a silent dissemination in the herd due to infected cell exchange, thus the concentration of BLV-infected cells in blood should play a major role in the success of viral transmission. Genes from Bovine leukocyte antigen (BoLA), the MHC system of cattle, are associated with genetic resistance and susceptibility to a wide range of diseases, and also with production traits. Some BoLA DRB3.2 allele polymorphisms in Holstein cattle have been associated with resistance or susceptibility to BLV-disease development, or with proviral load (PVL). This investigation studied 107 BLV-infected Argentinean Holstein dairy cows, all of them belonging to one herd. PVL was analysed by qPCR and animals were classified as high proviral load (HPVL, N = 88) and low proviral load (LPVL, N = 19), and BoLA DRB3.2 alleles were genotyped. Alleles BoLA DRB3.2*1501 and *1201 were significantly associated with HPVL (p = 0.0230 and p = 0.0111 respectively), while allele BoLA DRB3.2*0201 was significantly associated with LPVL (p = 0.0030). The present study aims at contributing to the knowledge of the association between BoLA polymorphism and development of a BLV infection profile. Genes that best explain the PVL in this population resulted BoLA DRB3.2*0201 (as a protection factor) and *1501 (as a risk factor). Allelic differences may play an important role in the development of effective immune responses. A better understanding of how BoLA polymorphism contributes to these responses and the establishment of a BLV status is desirable to schedule and evaluate control measures.
显示更多 [+] 显示较少 [-]A novel association of BoLA DRB3 alleles with BLV infected cattle with different proviral loads | Uma nova associação de alelos de BoLA DRB3 em bovinos infectados com BLV com diferentes cargas provirais 全文
2017
Nieto Farías, María Victoria | Caffaro, María Eugenia | Lendez, Pamela Anahí | Passucci, Juan Antonio | Poli, Mario Andres | Ceriani, Maria Carolina | Dolcini, Guillermina Laura
Bovine leukemia virus (BLV) is associated with the most common neoplastic disease of cattle. BLV has a silent dissemination in the herd due to infected cell exchange, thus the concentration of BLV-infected cells in blood should play a major role in the success of viral transmission. Genes from Bovine leukocyte antigen (BoLA), the MHC system of cattle, are associated with genetic resistance and susceptibility to a wide range of diseases, and also with production traits. Some BoLA DRB3.2 allele polymorphisms in Holstein cattle have been associated with resistance or susceptibility to BLV-disease development, or with proviral load (PVL). This investigation studied 107 BLV-infected Argentinean Holstein dairy cows, all of them belonging to one herd. PVL was analysed by qPCR and animals were classified as high proviral load (HPVL, N = 88) and low proviral load (LPVL, N = 19), and BoLA DRB3.2 alleles were genotyped. Alleles BoLA DRB3.2*1501 and *1201 were significantly associated with HPVL (p = 0.0230 and p = 0.0111 respectively), while allele BoLA DRB3.2*0201 was significantly associated with LPVL (p = 0.0030). The present study aims at contributing to the knowledge of the association between BoLA polymorphism and development of a BLV infection profile. Genes that best explain the PVL in this population resulted BoLA DRB3.2*0201 (as a protection factor) and *1501 (as a risk factor). Allelic differences may play an important role in the development of effective immune responses. A better understanding of how BoLA polymorphism contributes to these responses and the establishment of a BLV status is desirable to schedule and evaluate control measures. | O vírus da leucemia bovina (BLV) está associado à doença neoplásica mais comum do gado bovino. O BLV tem uma disseminação silenciosa no rebanho devido à troca de células infectadas, assim, a concentração de células BLV infectadas no sangue deve desempenhar um papel importante no sucesso da transmissão viral. Os genes do antígeno leucocitário bovino (BoLA), sistema MHC do gado bovino, estão associados à resistência genética e à susceptibilidade a uma ampla gama de doenças, bem como às características da produção. Alguns polimorfismos de alelos de BoLA DRB3.2 em bovinos Holstein têm sido associados à resistência ou susceptibilidade ao desenvolvimento da doença BLV, ou com carga proviral (PVL). Esta investigação avaliou 107 vacas leiteiras da raça Holstein argentina infectadas com BLV e pertencentes a um único rebanho. A PVL foi analisada por qPCR, os animais foram classificados em alta carga proviral (HPVL, N = 88) e baixa carga proviral (LPVL, N = 19), e os alelos BoLA DRB3.2 foram genotipados. Os alelos BoLA DRB3.2*1501 e *1201 estavam significativamente relacionados à HPVL (p = 0,0230 e p = 0,0111, respectivamente), enquanto o alelo BoLA DRB3.2*0201, à LPVL (p = 0,0030). O objetivo deste estudo é contribuir para o conhecimento da associação entre o polimorfismo de BoLA e o desenvolvimento de infecção por BLV. Os genes que melhor explicam a PVL na população analisada resultaram em BoLA DRB3.2*0201 (como fator de proteção) e *1501 (como fator de risco). As diferenças alélicas podem desempenhar um papel importante no desenvolvimento de respostas imunitárias eficazes. Uma melhor compreensão de como o polimorfismo BoLA contribui para estas respostas e o estabelecimento de um estado BLV é desejável para agendar e avaliar as medidas de controle. | Fil: Nieto Farías, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina | Fil: Caffaro, María Eugenia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina | Fil: Lendez, Pamela Anahí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina | Fil: Passucci, Juan Antonio. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina | Fil: Poli, Mario Andres. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina | Fil: Ceriani, Maria Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina | Fil: Dolcini, Guillermina Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
显示更多 [+] 显示较少 [-]Влияние натрия тиосульфата на морфологию костного мозга птиц при ассоциированной вакцинации против вирусных болезней
2009
Gromov, I.N. | Prudnikov, V.S., Vitebsk State Academy of Veterinary Medicine (Belarus) | Birman, B.Ya., National Academy of Sciences. Scientific and Practical Center of Animal Breding (Belarus). The S.N. Vyshelesskij Inst. of Experimental Veterinary Medicine
Investigation of morphological changes in bone marrow of young hens in the conditions of application of associated virus vaccines and sodium thiosulphate immunostimulant was realized in the Republic of Belarus. The associated virus vaccine was applied against infectious bursal disease, infectious bronchitis, infectious laryngotracheitis, and Newcastle disease. Research results showed that under the influence of inactivated vaccine against mentioned above diseases there was noted the increasing of quantity of granulocytic cells, there was stated an active hyperplasia of pseudoeosinophil cells. Immunization of young hens with the presented vaccine developed in the Institute of Experimental Veterinary Medicine of the National Academy of Sciences of Belarus promoted a short-term increasing of leucoerythroblastic index. Application of an alternative vaccine developed by Scientific and Production Enterprise Avivak did not render substantial influence on the morphology of bone marrow of chickens. Sodium thiosulphate applied in combination with associated vaccines (in 7% concentration in vaccine) caused the hyperplasia of lymphoid and thrombocyte cells, strengthened the proliferation and maturity of granular leukocytes
显示更多 [+] 显示较少 [-]Полиморфизм гена каппа-казеина (CSN3) в популяции крупного рогатого скота белоруской черно-пестрой породы
2009
Yatsyna, O.A. | Smuneva, V.K., Vitebsk State Academy of Veterinary Medicine (Belarus) | Epishko, T.I., Polesje State Univ., Pinsk (Belarus)
Determination of genetic structure of different population lines of Belarusian white-and-black breed in accordance with locus of kappa-casein gene (CSN3) was realized in the conditions of the Republic of Belarus. In course of study there were used DNA samples of Belarusian white-and-black breed cows (380 samples) and bioprobes of servicing bulls (83 samples). With the help of endonuclease Hindill there were revealed two alleles of kappa-casein (A and B) in DNA preparations. As a result of genetic typing of breeding animals by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) methods there were revealed three genotypes CSN3**AA, CSN3**AB, and CSN3**BB. The frequency of occurrence of desirable genotype CSN3**BB of Belarusian white-and-black breed of cows depending on breed and linear pertain varied from 2,2% up to 7,69%, for servicing bulls – from 0 up to 11,1% . The majority of homozygotic cows with undesirable cow genotype CSN3**AA belonged to the following lines: of Ruties Edward (2,31646 - 92,3%), Niko (86,0%), Vis Ajdal (933122 - 71,9%), Reflection Sovereign (198998 – 63,9%). The desirable CSN3**BB genotype in most of the cases was stated at Annas-Adema (30587) and Hiltes-Adema (37910) lines. Presence of heterozygote genotype predominated in Annas-Adema (46,1%) and Montvik Chiftein (36,3%) lines. Realized analysis of cow population lines of Danish and Holstein breeds showed that the average frequency of occurrence of CSN3**B allel was 17%, but lower concentration (15%) of CSN3**b allel was typical for the Danish breeds. Frequency of occurrence of CSN3**B allel in Holsten lines was 18%. In was revealed that in 86,6% of cases bulls were the genetic carriers of CSN3**A allel and only 11,4% of animals had the desirable allel CSN3**B in their genotype
显示更多 [+] 显示较少 [-]Плейотропное действие гена каппа-казеина (CSN3) на воспроизводительные качества быков-производителей
2010
Epishko, T.I., Polesje State Univ., Pinsk (Belarus) | Yatsyna, O.A. | Smuneva, V.K. | Yatsyna, V.V., Vitebsk State Academy of Veterinary Medicine (Belarus)
In the conditions of the Republic of Belarus there were analyzed indices of impregnating ability and efficiency of stud bull mothers of various genotypes on a kappa-casein gene (CSN3). As a result of genotyping of kappa-casein gene by DNA-diagnostics method it is revealed three genotypes - CSN3АА, CSN3AB, CSN3BB. Frequency of occurrence of homozygous genotype CSN3AA at stud bulls - 87,3%; heterozygotic CSN3AB - 20,5%; homozygous CSN3BB - 1,2%. Statistically authentic difference between these indices depending on a genotype it is not established. Thus, authentic connection between indices of impregnating ability and efficiency of stud bull mothers and their genotype on a locus of a kappa-casein gene that specifies in absence of influence of the last on the given indices is not established and gives the chance to conduct selection on gene CSN3 without decrease in breeding value of stud bulls on reproductive qualities.
显示更多 [+] 显示较少 [-]Взаимосвязь полиморфизма генов белков молока с показателями молочной продуктивности племенных коров белорусской черно-пестрой породы
2009
Gribanova, Zh.A., National Academy of Sciences. Scientific and Practical Center of Animal Breeding (Belarus)
Determination of influence of polymorphic variants of genes of kappa casein, beta-lactoglodulin and alpha-lactoalbumin on indexes of milk producing ability (milk yield, fat and protein content) of brood cows of Belarusian white-and-black breed was studied in the conditions of the Republic of Belarus. In course of analysis of polymorphism of kappa casein gene there was revealed the exceeding of frequency index of allel CSN3**a over the index of allel CSN3**b (0,795 and 0,205 respectively). Studying of polymorphism of beta-lactoglodulin gene showed that the frequency indexes of allel variants formed in the following way: BLG**a - 0,489 and BLG**b - 0,511. There were revealed three genotype variants of alpha-lactoalbumin among which the highest frequency indexes were stated for heterozygous genotype LALBA**aa *(32%) and LALBA**bb (14%). There was calculated the genetic equilibrium for loci of studied genes: x2 value for all studied loci was 0,008 – 1,96. Analysis of milk producing ability data in groups with different genotypes by locus of CSN3 gene showed that the highest indexes of milk yield were stated for group of cows with genotype CSN3**aa. Animals with genotype CSN3**bb had the highest fat percent, animals with genotype CSN3**aa - the lowest. Analysis of milk yield indexes showed that animals with homozygous genotypes BLG**aa and BLG**bb showed lower average milk yield than animals with genotype BLG**ab on 130 and 570 kg, respectively
显示更多 [+] 显示较少 [-]Влияние генотипа хряков по генам EPOR, MUC4 и ICF-2 на продуктивность потомства
2009
Dojlidov, V.A. | Kaspirovich, D.A., Vitebsk State Academy of Veterinary Medicine (Belarus) | Loban, N.A., National Academy of Sciences. Scientific and Practical Center of Animal Breeding (Belarus) | Bykova, M.I. | Mikhajlova, T.I., Selection and Hybrid Centre Zadneprovski (Belarus)
During the investigation of nuclear DNA of boas of Belarusian meaty breed there was studied the polymorphism of EPOR, MUC4 and IGF-2 genes with the further determination of frequency of alleles and genotypes. Among boars tested for the polymorphism EPOR gene there was stated high percentage of heterozygous samples (EPOR**st): 43,3% for Belarusian meaty breed boars and 66,7% for Belarusian Kripnaya Belaya breed. In course of analysis of the genetic structure by MUC4 gene there was revealed the predominance of animals with the desirable d allele combination (MUC**cc): 60% for Belarusian Kripnaya Belaya breed and 82,2% for Belarusian meaty breed; by IGF-2 gene with homozygotic manifestation of recessive alleles: 46,4% and 63,4%, respectively. In the conditions of the Republic of Belarus there were determined the preferred boar genotypes with EPOR, MUC4 and ICF-2 genes and combinations which made it possible to increase the fattening and meaty qualities of posterity. The research results showed that in the selection breeding schemes it was necessary to consider not only maternal genotype, but also male genotypes giving preference to EPOR**st, MUC4**ss and ICF-2**qq genotypes
显示更多 [+] 显示较少 [-]Взаимосвязь между основными показателями молочной продуктивности крупного рогатого скота и различными генотипами каппа-казеина
2010
Tanana, L.A., Grodno State Agrarian Univ. (Belarus) | Peshko, V.V., Grodno State Agrarian Univ. (Belarus) | Epishko, T.I., Polesje State Univ., Pinsk (Belarus)
In the conditions of the Republic of Belarus there was analyzed the interrelation between the main indexes of cattle milk performance of dairy cattle of Large Red Belarusian breed and Belarusian Black-and-White breed with different genotypes of kappa casein gene. In course of the study there was determined the frequency of occurrence of genotypes and alleles by kappa-casein gene in population of cows of mentioned above breeds. Values of correlation coefficients between some indexes of milk performance of the studied breeds were analyzed. Correlation factors between the basic indexes of milk performance of cows with different genotypes of kappa casein. Research results showed that milk protein content and butter-fat yielding capacity were in negative association with the main breeding trait of dairy cattle – amount of milk yield. Butter-fat yielding capacity and milk protein content correlated positively. Thus, selection breeding in accordance with one of this traits will promote increasing of the other. Besides, it is necessary to realize DNA-diagnostics of cattle according to kappa casein gene and to select animals having in their genomes allele CSN3**B connected with higher milk protein content.
显示更多 [+] 显示较少 [-]Влияние генотипа хряков белорусской крупной белой и белорусской мясной породы по гену MUC4 на сохранность и продуктивность потомства
2009
Kaspirovich, D.A. | Dojlidov, V.A., Vitebsk State Academy of Veterinary Medicine (Belarus) | Loban, N.A., National Academy of Sciences. Scientific and Practical Center of Animal Breeding (Belarus) | Bykova, M.A. | Mikhajlova, T.A., Selection and Hybrid Centre Zadneprovski (Belarus)
Analysis of influence of boar genotype by MUC4 gene on viability of progeny was realized in the conditions in the Republic of Belarus, as well as the evaluation of possibilities of association of polymorphic variants of gene with fattening and meaty qualities of obtained from the boars progeny were conducted. In course of studies there was analyzed the polymorphism of MUC4 gene at producer boars of studied breeds. There was revealed high occurring frequency of desirable allele MUC4**c and MUC4**cc genotype in all studied populations. Boars of Belarusian Meaty breed with genotypes MUC4**cc by MUC4 gene authentically (P less than 0,01) exceeded boars with genotype MUC4**cg according to piglet viability indexes at weaning period on 5,6%. For the further evaluation of the potential role of MUC4gene in marker selection there was realized determination of relations between genotypes of studied marker and other economic traits of swine (fattening and meaty traits of boar progeny). There was stated the authentic increasing of fattening and meaty traits of stores obtained from boars with homozygous genotype MUC4**cc in comparison with progeny fathers of which in their genotype had mutant allel MUC4**g. For the forecasting and modeling of genotypes resistant to colibacillosis there was presented a scheme of selection boars and sows of different genotypes by MUC4 gene for the increasing of progeny viability. According to the scheme the preferred selection was MUC4**cc x MUC4**cc. This selection was recommended for the self-replacement and creation of nuclear stock
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