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Improvement of indirect enzyme-linked immunosorbent assay for detection of Japanese encephalitis virus antibodies in swine sera
2017
Yang, D.K., Viral Disease Division,Animal and Plant Quarantine Agency, Gimcheon, Republic of Korea | Kim, H.H., Viral Disease Division,Animal and Plant Quarantine Agency, Gimcheon, Republic of Korea | Jo, H.Y., Viral Disease Division,Animal and Plant Quarantine Agency, Gimcheon, Republic of Korea | Lee, S.H., Viral Disease Division,Animal and Plant Quarantine Agency, Gimcheon, Republic of Korea | Jang, S.H., R and D Center, MEDIAN Diagnostics, Chuncheon, Republic of Korea | Lee, S.O., R and D Center, MEDIAN Diagnostics, Chuncheon, Republic of Korea | Choi, S.S., Viral Disease Division,Animal and Plant Quarantine Agency, Gimcheon, Republic of Korea | Cho, I.S., Viral Disease Division,Animal and Plant Quarantine Agency, Gimcheon, Republic of Korea
Japanese encephalitis (JE) is an important zoonosis caused by the mosquito-transmitted JE virus (JEV), which is a causative agent of reproductive failure in pregnant sows. Detection of JEV antibodies in swine is performed by hemagglutination inhibition (HI), virus neutralization (VN), and the plaque reduction neutralization test (PRNT). The most stringent PRNT is the 90% endpoint PRNT (PRNT∧90). These conventional assays are difficult to carry out in diagnostic laboratories with insufficient instruments or cell culture systems. An alternative assay that is easily conducted and time efficient is required. In this study, we improved the indirect enzyme-linked immunosorbent assay (I-ELISA) with clarified antigen for the detection of JEV antibodies. The I-ELISA results obtained from 175 swine serum samples were compared with HI, VN, and PRNT∧90 results. The sensitivity of I-ELISA was 91.8%, 95.0%, and 94.7% compared with HI, VN, and PRNT∧90 results, respectively. The specificity of I-ELISA was 92.2%, 94.7%, and 94.7% compared with HI, VN, and PRNT∧90 results, respectively. Moreover, the I-ELISA results were significantly correlated with the HI (r = 0.93), VN (r = 0.95), and PRN∧T90 (r = 0.92) results. These results suggest that the improved I-ELISA is useful for serosurveillance of JEV in swine.
显示更多 [+] 显示较少 [-]Gonadotropin-releasing hormone immunization and castration in male pigs: effects on growth, hormonal levels, antibody titer response, testicular function, back fat, and consumers' sensory perceptions
2017
Yamsakul, P., Chiang Mai University, Chiang Mai, Thailand | Patchanee, P., Chiang Mai University, Chiang Mai, Thailand | Yano, T., Chiang Mai University, Chiang Mai, Thailand | Boonma, T., Chiang Mai University, Chiang Mai, Thailand | Somkert, C., Chiang Mai University, Chiang Mai, Thailand | Sathanawongs, A., Chiang Mai University, Chiang Mai, Thailand
This study investigated the effects of gonadotropin-releasing hormone (GnRH) vaccine on pig growth, carcass quality, and sensory perceptions of pork meat by Thai consumers. Male crossbred pigs (n = 30) were separated into three groups of 10 pigs each: boars, immunocastrated pigs, and surgically castrated pigs. The immunocastrated group was immunized at 13 and 19 weeks of age with GnRH vaccine (400 킽/dose). All pigs were slaughtered at 24 weeks of age. Blood samples were collected and testes size determined. Testes weight and back fat thickness were recorded at the time of slaughter, and meat samples were collected for sensory assessment by Thai consumers. Testosterone and progesterone concentration levels decreased significantly two weeks after the second dose of GnRH (p less than 0.05), and the GnRH antibody titer of the immunocastrated pigs was significantly high two weeks after the second dose of GnRH (p less than 0.05). GnRH vaccine significantly reduced the weight and size of testes. Thai consumers reported no significant differences in odor or flavor among meat samples from the three groups. In conclusion, immunocastration improved growth performance, removed the need for castration surgery, and avoided "boar taint" in the meat. It has also no distinct trace of the boar taint same as pork that is currently produced from physically castrated pig which is making it acceptable to consumers.
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