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Blast transformation of adherent macrophages infected in vitro with sporozoites of Theileria parva
1984
Moulton, J. | Büscher, Gottfried | Bovell, D.L. | Doxsey, Stephen J.
Attempt to pharmacologically modulate procoagulant activity of lipopolysaccharide-stimulated adherent bovine alveolar macrophages.
1996
Olchowy T.W.J. | Dean D.F. | Bochsler P.N.
Equine herpesvirus 2 in pulmonary macrophages of horses.
1995
Schlocker N. | Fellenberg R. von
In a search of viral agents in pulmonary macrophages of horses with chronic pulmonary disease, equine herpesvirus 2 was found to be unique. In 8 of 9 horses with chronic pulmonary disease, antigens of equine herpesvirus 2 were detected by indirect immunofluorescence staining of scattered foamy macrophages immediately after harvesting by bronchoalveolar lavage and fractionation on metrizamide gradients. In a healthy horse, antigens were not found. After 1 week of cultivation of bronchoalveolar lavage cells from a second group of 9 horses with chronic pulmonary disease, viral antigens were detected in 90% of the adherent pulmonary macrophages. In 2 of 3 healthy horses, viral antigens also were found in 90% of the adherent pulmonary macrophages. Antigens of equine herpesvirus 1, equine herpesvirus 4, parainfluenza virus 3, or adenovirus were not detected. Antigens of the 5 investigated viruses also were not detected in lung tissue slices from a third group of 14 horses, 4 healthy; 7 with varying degrees of bronchiolitis, 2 of which also had chronic intestitial pneumonia; 2 with eosinophilic bronchitis; and 1 with pulmonary hemorrhage. The exclusive presence of equine herpesvirus 2 in pulmonary macrophages was confirmed qualitatively by isolation of infective virus by cocultivation. In a fourth group of 12 horses with chronic pulmonary disease, infective virus could be isolated from pulmonary macrophages of 3 horses and from mixed-blood leukocytes of 5 horses. Virus isolations from 2 healthy horses were not successful from pulmonary macrophages, whereas 1 isolation was obtained from mixed-blood leukocytes. Other viruses were not detected by cocultivation.
显示更多 [+] 显示较少 [-]Effect of PHA and conditioned medium on blastogenesis and rosette formation of bovine circulating blood lymphocytes.
1994
Kang S.W. | Yoon C.Y. | Song H.J.
Activity and application of 20alpha-hydroxysteroid dehydrogenase in rat-(2)-Changes in activities of the splenic macrophages and ovarian histological findings.
1994
Kang C.B. | Kwak S.D.
Semi-stable production of bovine IL-4 and GM-CSF in the mammalian episomal expression system 全文
2021
Blanco, Federico Carlos | Vazquez, Cristina Lourdes | García, Julia Sabio y | Rocha, Rosana Valeria | Gravisaco, María José | Forrellad, Marina Andrea | Magistrelli, Giovanni | Bigi, Fabiana
Semi-stable production of bovine IL-4 and GM-CSF in the mammalian episomal expression system 全文
2021
Blanco, Federico Carlos | Vazquez, Cristina Lourdes | García, Julia Sabio y | Rocha, Rosana Valeria | Gravisaco, María José | Forrellad, Marina Andrea | Magistrelli, Giovanni | Bigi, Fabiana
Granulocyte-macrophage colony stimulating factor (GM-CSF) and interleukin-4 (IL-4) are cytokines widely used in ex vivo monocyte differentiation experiments, vaccine formulations and disease treatment. The aim of this study was to produce recombinant bovine GM-CSF and IL-4 in an episomal expression system that conserves the postransductional modification of the native proteins and to use the products to differentiate bovine monocytes into dendritic cells. The recombinant proteins rGM-CSF and rIL-4 were expressed in PEAKrapid CRL-2828 human kidney cells, ATCC CRL-2828. The functional activity of the recombinant cytokines was monitored by registering morphological changes in bovine monocytes and assessing the expression of CD14 upon incubation with them. Both recombinant proteins were detected in the cell culture supernatant of transfected cells. Culture supernatants of transfected cells induced in bovine monocytes morphological changes that resemble macrophages or dendritic cells. In addition, bovine cells treated with rGM-CSF and rIL-4 showed reduced expression of the macrophage surface marker CD14 compared with untreated cells. This effect indicates the expected differentiation. The expression of the cytokines was stable after many successive cell passages and a freeze/thaw cycle. The semi-stable mammalian episomal expression system used in this study allowed us to easily produce functional bovine rGM-CSF and rIL-4 without the need for protein purification steps.
显示更多 [+] 显示较少 [-]Semi-stable production of bovine IL-4 and GM-CSF in the mammalian episomal expression system 全文
2021
Blanco Federico Carlos | Vazquez Cristina Lourdes | García Julia Sabio y | Rocha Rosana Valeria | Gravisaco María José | Forrellad Marina Andrea | Magistrelli Giovanni | Bigi Fabiana
Granulocyte-macrophage colony stimulating factor (GM-CSF) and interleukin-4 (IL-4) are cytokines widely used in ex vivo monocyte differentiation experiments, vaccine formulations and disease treatment. The aim of this study was to produce recombinant bovine GM-CSF and IL-4 in an episomal expression system that conserves the postransductional modification of the native proteins and to use the products to differentiate bovine monocytes into dendritic cells.
显示更多 [+] 显示较少 [-]The protective action of piperlongumine against mycobacterial pulmonary tuberculosis in its mitigation of inflammation and macrophage infiltration in male BALB/c mice 全文
2021
Lu Nihong | Yang Yongrui | Li Xiaofei | Li Jie | Cheng Jie | Lv Zhengxuan | Du Yingrong
The protective action of piperlongumine against mycobacterial pulmonary tuberculosis in its mitigation of inflammation and macrophage infiltration in male BALB/c mice 全文
2021
Lu Nihong | Yang Yongrui | Li Xiaofei | Li Jie | Cheng Jie | Lv Zhengxuan | Du Yingrong
Piperlongumine (PL) is a bioactive alkaloid and medicinal compound of piperamide isolated from the long pepper (Piper longum Linn). It has demonstrated bactericidal action against Mycobacterium tuberculosis (MTB), the cause of pulmonary tuberculosis; nevertheless, immunomodulatory activity had not been identified for it in MTB-triggered granulomatous inflammation. This study investigated if piperlongumine could inhibit such inflammation.
显示更多 [+] 显示较少 [-]The protective action of piperlongumine against mycobacterial pulmonary tuberculosis in its mitigation of inflammation and macrophage infiltration in male BALB/c mice 全文
2021
Lu, Nihong | Yang, Yongrui | Li, Xiaofei | Li, Jie | Cheng, Jie | Lv, Zhengxuan | Du, Yingrong
Piperlongumine (PL) is a bioactive alkaloid and medicinal compound of piperamide isolated from the long pepper (Piper longum Linn). It has demonstrated bactericidal action against Mycobacterium tuberculosis (MTB), the cause of pulmonary tuberculosis; nevertheless, immunomodulatory activity had not been identified for it in MTB-triggered granulomatous inflammation. This study investigated if piperlongumine could inhibit such inflammation. Mycobacterium tuberculosis strain H37Rv was subjected to a broth microdilution assay. Piperlongumine at 5, 15, and 25 μg/mL, 0.2% dimethyl sulphoxide as control or 4 μM of dexamethasone were tested in vitro on MH-S murine alveolar macrophages. BALB/c mice were orally administered PL at 50, 100 and 150 mg/kg b.w. after trehalose-6,6-dimycolate (TDM) stimulation. Chemokine and cytokine concentrations were determined in lung supernatants. Flow cytometry and Western blot analysis were performed to determine phosphorylated spleen tyrosine kinase (Syk), c-Jun N-terminal kinase (JNK) and extracellular signal-regulated kinase (ERK) pathways. Piperlongumine inhibited inflammatory mediators and adherence of lymphocyte function-associated antigen 1 to MH-S cells following TDM activation. It also improved macrophage clearance of MTB. In TDM-stimulated MH-S cells, PL significantly influenced the macrophage inducible Ca²⁺-dependent lectin receptor (Mincle)-Syk-ERK signalling pathway. Oral dosing of PL effectively suppressed the development of pulmonary granulomas and inflammatory reactions in the TDM-elicited mouse granuloma model. PL as an inhibitor of MTB-triggered granulomatous inflammation may be an effective complementary treatment for mycobacterial infection.
显示更多 [+] 显示较少 [-]Construction and activity analyses of single functional mouse peroxiredoxin 6 (Prdx6) 全文
2019
Wang, Lu-Lu | Lu, Shi-Ying | Hu, Pan | Fu, Bao-Quan | Li, Yan-Song | Zhai, Fei-Fei | Ju, Dan-Di | Zhang, Shi-Jun | Su, Bing | Zhou, Yu | Liu, Zeng-Shan | Ren, Hong-Lin
Construction and activity analyses of single functional mouse peroxiredoxin 6 (Prdx6) 全文
2019
Wang, Lu-Lu | Lu, Shi-Ying | Hu, Pan | Fu, Bao-Quan | Li, Yan-Song | Zhai, Fei-Fei | Ju, Dan-Di | Zhang, Shi-Jun | Su, Bing | Zhou, Yu | Liu, Zeng-Shan | Ren, Hong-Lin
Introduction: Peroxiredoxin 6 (Prdx6) is a bifunctional protein with glutathione peroxidase activity and phospholipase A2 activity. Previous studies have shown a significant positive correlation between the intracellular survival ability of Brucella and Prdx6. Here, the Prdx6 enzyme with a single activity was constructed to facilitate study of the relationship between the single function of Prdx6 and Brucella infection. Material and Methods: The target open reading frame (ORF) DNAs of Prdx6 with a single active centre were prepared using gene splicing by overlap extension PCR (SOE-PCR), and the recombinant eukaryotic expression plasmids inserted by Prdx6 with the single activity centre were constructed and transfected into murine Raw264.7 macrophages. The glutathione peroxidase activity and phospholipase A2 activity of the constructed Prdx6 were examined. Results: The core centres (Ser³² and Cys⁴⁷) of Prdx6 were successfully mutated by changing the 94ᵗʰ nucleotide from T to G and the 140ᵗʰ nucleotide from G to C in the two enzyme activity cores, respectively. The constructed recombinant plasmids of Prdx6 with the single active centre were transfected into murine macrophages showing the expected single functional enzyme activity, which MJ33 or mercaptosuccinate inhibitors were able to inhibit. Conclusion: The constructed mutants of Prdx6 with the single activity cores will be a benefit to further study of the biological function of Prdx6 with different enzyme activity.
显示更多 [+] 显示较少 [-]Construction and activity analyses of single functional mouse peroxiredoxin 6 (Prdx6) 全文
2019
Wang Lu-Lu | Lu Shi-Ying | Hu Pan | Fu Bao-Quan | Li Yan-Song | Zhai Fei-Fei | Ju Dan-Di | Zhang Shi-Jun | Su Bing | Zhou Yu | Liu Zeng-Shan | Ren Hong-Lin
Introduction: Peroxiredoxin 6 (Prdx6) is a bifunctional protein with glutathione peroxidase activity and phospholipase A2 activity. Previous studies have shown a significant positive correlation between the intracellular survival ability of Brucella and Prdx6. Here, the Prdx6 enzyme with a single activity was constructed to facilitate study of the relationship between the single function of Prdx6 and Brucella infection.
显示更多 [+] 显示较少 [-]Fasciola hepatica isolates induce different immune responses in unmaturated bovine macrophages 全文
2019
Bąska, Piotr | Zawistowska-Deniziak, Anna | Norbury, Luke James | Wiśniewski, Marcin | Januszkiewicz, Kamil
Fasciola hepatica isolates induce different immune responses in unmaturated bovine macrophages 全文
2019
Bąska, Piotr | Zawistowska-Deniziak, Anna | Norbury, Luke James | Wiśniewski, Marcin | Januszkiewicz, Kamil
Introduction: Fasciola hepatica (liver fluke) is a parasite of great socioeconomic importance. A number of fluke isolates have been identified; however, to date the differences between the immunomodulatory properties of different parasite isolates have not been sufficiently investigated. The aim of this study was to explore differences between the immunomodulatory properties of two F. hepatica isolates using unmaturated bovine macrophages. Material and Methods: A cell line of bovine macrophages was stimulated with excretory/secretory products released by adult flukes from either a laboratory (Fh-WeyES) or wild (Fh-WildES) strain and subsequently subjected to microarray and ELISA analyses. Results: Both Fh-WeyES and Fh-WildES dampened the release of interleukin-10 by bovine macrophages, but only Fh-WildES dampened the release of proinflammatory tumour necrosis factor-α. Microarray analysis revealed that Fh-WildES down- and upregulated 90 and 18 genes, respectively, when compared to Fh-WeyES. Conclusion: The results indicated different impacts of the isolates on macrophages. A number of researchers use flukes obtained from local slaughterhouses for experiments. Our findings may explain some discrepancies between published results arising from parasite strain choice. The findings indicate that consideration should be given to the use of different strains, and open new and currently unexplored avenues in parasitology for controlling the parasite.
显示更多 [+] 显示较少 [-]Fasciola hepatica isolates induce different immune responses in unmaturated bovine macrophages 全文
2019
Bąska Piotr | Zawistowska-Deniziak Anna | Norbury Luke James | Wiśniewski Marcin | Januszkiewicz Kamil
Introduction:Fasciola hepatica (liver fluke) is a parasite of great socioeconomic importance. A number of fluke isolates have been identified; however, to date the differences between the immunomodulatory properties of different parasite isolates have not been sufficiently investigated. The aim of this study was to explore differences between the immunomodulatory properties of two F. hepatica isolates using unmaturated bovine macrophages.
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