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Attempted transmission of Ehrlichia canis by Rhipicephalus sanguineus after passage in cell culture.
1996
Mathew J.S. | Ewing S.A. | Barker R.W. | Fox J.C. | Dawson J.E. | Warner C.K. | Murphy G.L. | Kocan K.M.
Complete primary sequence of equine cartilage link protein deduced from complementary DNA.
1995
Dudhia J. | Platt D.
Investigation of the structure of equine articular cartilage link protein (LP) from individuals ranging in age from 1 to 15 years identified 3 distinct isoforms having molecular weights of 46,000, 43,000, and 41,000. The relative amounts of each of the 3 isoforms altered with age. The largest form did not change with age; however, amounts of the Mr 43,000 and 41,000 forms increased with increasing age. The results suggested that an accumulation, in the extracellular matrix of cartilage, of these 2 smaller products may have arisen from proteolytic cleavage. The complete amino acid sequence of the protein core was determined from complementary DNA products prepared by polymerase chain reaction amplification of cartilage LP mRNA. The sequence had 96% similarity with human LP and with that of other species for which the primary structure has been determined. This high degree of sequence conservation and the isoform data indicate that extracellular processing of LP occurs by similar mechanisms in various species. At the transcription level, equine chondrocytes were found to express LP as 2 abundant mRNA of 5.0 and 3.0 kb, and a smaller mRNA of 1.5 kb. Processing of the LP mRNA in horses, thus, appears to be similar to that found in other species investigated, and although multiple transcripts are present, the coding region remains unaltered and only 1 protein product is made.
显示更多 [+] 显示较少 [-]Investigation of the 18S rRNA gene sequence of Hepatozoon canis detected in Indian dogs 全文
2017
Bal, M. S. | Sumbria, Deepak | Kaur, Paramjit | Singla, L. D. | Mandhotra, Ajay
Canine hepatozoonosis isa growing tick-borne disease in Punjab.Two canine hepatozoonosis cases, oneclinical and one subclinical, in Punjabwere analyzed by PCR targeting 18S rRNAgene (666 bp). After sequence analysisof the PCR products, both of them werefound almost identical to each other andwere closely related to the Hepatozooncanis strain found in Saint kitts and Nevisand Brazil with 100% (442/442) and 99%(440/442) nucleotide identity respectively.Isolates from Malta and Philippines ofH. canis were distantly related to IndianH. canis with 437/442 and 436/442 matchidentities. These results suggest that H.canis detected in north Indian dogs mighthave closer ancestral relationship with SaintKitts and Nevis followed by Brazil strain.This is the first molecular characterizationof Hepatozoon from Punjab, India.
显示更多 [+] 显示较少 [-]Characterization of Akabane virus (KV0505) from cattle in Korea
2008
Yang, D.K. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea), E-mail: [email protected] | Kim, Y.H. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Kim, B.H. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Kweon, C.H. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Yoon, S.S. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Song, J.Y. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Lee, S.H. (Jeju Veterinary Research Institute, Jeju, Republic of Korea)
Akabane disease is caused by an arthropod-borne viral pathogen and leads congenital abnormalities of the central nervous system in infected ruminants. One isolate, KV0505, showed cytopathic effect in Vero cells. The KV0505 isolate was obtained from plasma, which was collected from a cattle raised on Jeju Island in May 2005. Jeju Island is located near the southern part of the Korean peninsula. The isolate was confirmed as Akabane virus (AKAV) by immunofluorescence assay using AKAV specific monoclonal antibodies and reverse transcription polymerase chain reaction (RT-PCR). Suckling mice inoculated with the isolate showed signs of paralysis and died within 10 days post-inoculation. Comparisons of the KV0505 N gene sequence with 39 other known AKAV strains revealed nucleotide homologies ranging from 83.6% (MP496 strain) to 99.7% (M171 strain). When compared with the K-9 strain, which was isolated from a cow in Korea in 1994, the nucleotide sequence homology with the N gene was 99.7%. Thus, genes of the KV0505 isolate were closely related to those of the M171 strain, which were clustered into the Ic group of AKAV.
显示更多 [+] 显示较少 [-]Genogroup position of aquabirnavirus GC-1 isolated from rockfish Sebastes schlegeli in Korea
2008
Joh, S.J. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea), E-mail: [email protected] | Lee, Y.J. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Song, C.S. (Konkuk University, Seoul, Republic of Korea) | Kang, S.Y. (Chungbuk National University, Cheongju, Republic of Korea) | Mo, I.P. (Chungbuk National University, Cheongju, Republic of Korea) | Heo, G.J. (Chungbuk National University, Cheongju, Republic of Korea)
The cDNA of the aquabirnavirus, GC-1 isolated from rockfish Sebastes schlegeli in Korea, was synthesized using the reverse transcriptase-polymerase chain reaction. The nucleotide and deduced amino acid sequences were determined from cDNA of the VP2-NS-VP3 coding region of genome segment A. The nucleotide sequences of the segment A were 3,086 base pairs (bp) in length and contained large open reading frame (ORF) and terminal sequences. The large ORF was comprised of 2,916 bp nucleotides and composed of 972 deduced amino acid sequences. Pairwise comparisons were made with other aquabirnavirus sequences published previously. The study of genetic relationships between GC-1 and aquabirnaviruses in the large ORF and VP2 coding regions demonstrated that the GC-1 has the nearest genetic relationship with the marine birnaviruses (MABV strains), and the GC-1 and MABV strains can be clustered as the same genogroup. GC-1 can be included in MABV, which is the 7th genogroup of family Aquabirnaviridae.
显示更多 [+] 显示较少 [-]Genetic characterization of peste des petits ruminants virus circulating in different regions of Pakistan based on nucleocapsid gene sequence 全文
2019
Usman, M. ((National Agricultural Research Center (NARC), Islamabad (Pakistan). Animal Sciences Institute, Animal Health Research Laboratories), (Quaid-i-Azam University, Islamabad (Pakistan). Department of Animal Sciences)) | Ahsan, A. | Rasheed, T. | Farooq, U. | Ameen, M.K. | Zahur, A.B. | Khan, S.H.
Multi-locus exon-primed intron crossing (EPIC) primer design for regional birds and algorithm design for a combination of introns 全文
2018
Kumakura, K. (Rakuno Gakuen University, Ebetsu, Hokkaido (Japan). School of Veterinary Medicine, Laboratory of Radiation Biology) | Osanai, Y. | Osa, Y. | Fujii, K. | Asakawa, M. | Ushiyama, K. | Onuma, M. | Igarashi, H. | Endoh, D.
Universal nematode detection by degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR) of purified nematode nucleic acids 全文
2018
Yoshino, T. (Rakuno Gakuen University, Ebetsu, Hokkaido (Japan). School of Veterinary Medicine, Laboratory of Parasitology) | Asakawa, M. | Osa, Y. | Kaneko, M. | Endoh, D.
Discovery of genome of an immunodeficiency-associated virus-like virus from pig feces in Japan 全文
2018
Oba, M. (Tokyo University of Agriculture and Technology, Tokyo (Japan). Research and Education Center for Prevention of Global Infectious Diseases of Animals) | Katayama, Y. | Tsuchiaka, S. | Omatsu, T. | Murata, Y. | Ohya, K. | Makino, S. | Nagai, M. | Mizutani, T.
Application of next-generation sequencing to investigation of norovirus diversity in shellfish collected from two coastal sites in Japan from 2013 to 2014 全文
2016
Imamura, S. (Ministry of Agriculture, Forestry and Fisheries, Chiyoda-ku, Tokyo (Japan). Food Safety Consumer Affairs Bureau) | Haruna, M. | Goshima, T. | Kanezashi, H. | Okada, T. | Akimoto, K.