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Bioassay techniques and high-performance liquid chromatography for detection of oxytetracycline residues in tissues from calves.
1989
MacNeil J.D. | Korsrud G.O. | Naylor J.M. | Yates W.D.G.
Tissue specimens from muscle, liver, kidney, and injection sites were collected, and serum was obtained from 3 calves euthanatized on each of posttreatment days 5 and 22. Calves were treated with 6.7, 13.4, or 20 mg of oxytetracycline (OTC)/kg of body weight, IM, once daily for 3 days; these dosages are 1, 2, and 3 times the label dose, respectively. One control calf was euthanatized on each of posttreatment days 5 and 22. In treated male calves killed 2 days after the last injection, OTC residues were detected in all tissues and serum, using high-performance liquid chromatography. Tissues from all injection sites also were considered positive for antimicrobial residues, using swab test on premises (STOP), microbial inhibition test (MIT), and thin-layer chromatography-biautography (TLCB) test. Kidney tissues from a calf given 13.4 mg of OTC/kg and kidney and liver tissues from a calf given 20 mg of OTC/kg also were considered positive, using the MIT and TLCB. Results of the STOP only were considered positive for the liver and kidney of a calf given 20 mg of OTC/kg, but substitution of Saskatoon antibiotic medium-3 for the original medium (antibiotic medium-5) allowed the STOP to detect residues in these tissues from all treated calves. In female calves killed 19 days after the last injection, the STOP, MIT, and TLCB procedures revealed positive results for tissues from some injection sites, but revealed negative results for other tissues. High-performance liquid chromatographic analyses detected OTC in tissues from injection sites from all treated calves, in muscle and liver from a calf given 20 mg of OTC/kg, and in kidneys from calves given 13.4 or 20 mg of OTC/kg. The STOP, MIT, and TLCB procedures lacked the sensitivity of high-performance liquid chromatography for detection of OTC residues.
显示更多 [+] 显示较少 [-]Species characterization of animal by DNA hybridization
1999
Lee, M.H. | Kim, S.K. (Chungnam National University, Taejon (Korea Republic). College of Veterinary Medicine) | Jung, G.S. | Park, J.M. (National Veterinary Research & Quarantine Service, Anyang (Korea Republic).)
DNA hybridization assay using probes prepared from liver was carried out to identify species characterization of the domestic animals. Gel electrophoresis showed that the target DNA extracted from raw muscle were 1kb and uniform pattern while fragments size of heated muscle were irrgular. Hybridizatino was performed by adding 200ng/ml probe in hybridization solution and incubating for 12 hours at 68 degrees centigrade. To obtain good discrimination, applied washing buffer and washing step differently depending on the species. The probes of pig, horse and dog formed the specific hybrids with each target DNA respectively. Although cross reaction ws detected in cattle, goat and sheep but signal intensity among these species made the discrimination possible each other. Such pattern was the same in the cases of chicken, turkey and duck. The hybridization pattern of heated muscle was similar to that of raw muscle in general, but the signal intensity was inferior to that of raw muscle. Species identification between closely related animal species, hybridized using the target DNA of such closely related animal species as a blocking agent, remarkable increase of discrimination from the evident decrease of non specific reaction compared with the control group. In addition, in the admixture where certain meat was included in the beef, pork, chicken meat, we colud find whether any unjust meat was admixed or not. In this case, detection limit of certain meat in admixture was 1%.
显示更多 [+] 显示较少 [-]Selective culture media for the recovery of Aeromonas spp from poultry meat.
2011
Smita, | Bhong, C. D. | Brahmbhatt, M.N.
Different selective culture media were evaluated for isolation of Aeromonas spp. from 120 poultry meat samples. The recovery ofAeromonas isolates was highest fromAmpicillin DextrinAgar (89.39%), followed by Aeromonas Starch DNAse agar (68.18 %) andAeromonas isolation media (18.18 %).
显示更多 [+] 显示较少 [-]Carcass Quality and Sensory Properties of Guinea Fowls and Broiler Chicken Meat.
2009
Singh, A.K. | Motina, E. | Singh, N. S. | Verma, D. N.
Comparision of the carcass traits and sensory properties of meat between different genetic groups of guinea fowl and broiler chickens at 16 weeks ofa gerevealed that the dressing and eviscerated weight percentage of broiler chickens were significantly lower from that all the guinea fowl groups, except from that of Pearl, where the differences were not significant. Percent giblet weight was lower (P of weeks 16 at meat raw properties sensory their in chickens broiler than grades higher scored fowls guinea general, In weights. wing and leg cent per for except ofcarcass cuts birds groups between observed were differences No cross. ofLXP that from only significant but (67.77%), lowest also was yield Similarly, fowls.
显示更多 [+] 显示较少 [-]Оценка токсичности комплексного препарата для лечения алиментарной анемии и его влияние на качественные показатели мяса свиней
2010
Dremach, G.Eh. | Zajtseva, A.V., Vitebsk State Academy of Veterinary Medicine (Belarus)
Belarus concerns a biogeochemical province with the lowered content in environment of such microelements, as iodine, selenium, copper, cobalt, manganese, zinc and some other. It conducts to infringement of intensity and an orientation of processes of an exchange of proteins, carbohydrates, lipids, and finally - to decrease in their growth and development of an organism of young growth. High cost of foreign preparations for treatment and preventive maintenance of an alimentary anaemia, low bioavailability of iron and toxicity do not provide purposeful disesase control and immunodeficiencies arising on its background. The special urgency is got by research of means of complex preventive maintenance of an alimentary anaemia and immunodeficiencies. It has been established that the recipes N1-3 have proved to be non-toxic for laboratory animals and have no effect on the meat quality.
显示更多 [+] 显示较少 [-]К вопросу о доброкачественности и безопасности продуктов убоя крупного рогатого скота при хламидиозе
2010
Fomchenko, I.V., Vitebsk State Academy of Veterinary Medicine (Belarus)
Analysis of veterinary and sanitary examination and quality of cattle slaughter products after cattle clamidiosis was realized in the conditions of the Republic of Belarus from April, 2004 till January, 2006. Over all these research periods there were studied 1579 cattle heads and their slaughter products. The usual salting of meat and other products with the purpose of their clearing from chlamydiosis was unsuitable for practical application. Due to long-term (1,5-9 months) persistence of chlamydia in parenchymal organs for the prevention of people infection and chlamydiosis spreading it is necessary to realize slaughter of diseased animals at a diseased animal slaughter house or in a killing department at the end of working shift with observance of measures of individual preventive maintenance and the subsequent disinfection of a premise, the equipment, stock and tools.
显示更多 [+] 显示较少 [-]Мясная продуктивность помесных герефорд х черно-пестрых телок в зависимости от содержания концентратов в рационе
2010
Zhdanova, A.A., Vitebsk State Academy of Veterinary Medicine (Belarus)
In the conditions of the Republic of Belarus there was realized a comparative study of meat productivity of mongrel Gereford х black-and-white heifers ad purebred black-and-white heifers in the conditions of different levels of concentrated feedstuff in cattle diets; and determine the most efficient and long-term method of animal breeding for meat. Research results showed that breeding of meaty of hybrids of Gereford х black-and-white heifers of the second generation without using the feeding concentrates in their diets or on using rations with a low rate of feeding concentrates (10-20%) proved to be more effective, than breeding of purebred black-and-white herdmates (for which specific density of the concentrated forages in diets made up 20% through pregnancy). Hybrids on Gereford breed in accordance with meat productivity level surpassed the purebred black-and-white animals. The most considerable results were marked at hybrid heifers with relative density of concentrates in a ration of 20% which in accordance with slaughter weight indexes surpassed the herdmates of black-and-white breed on 39,1 kg or on 23,68%, in accordance with slaughter yield - on 8,2%, in accordance with fresh carcass weight - on 37,9 kg or on 23,8%, in accordance with weight of inner porc - on 1,19 kg or on 20,62%.
显示更多 [+] 显示较少 [-]Формирование мясной продуктивности и синтез белка, жира в тканях тела бычков при повышенном уровне энергии в рационе
2010
Tsaj, V.P. | Lemeshevskij, V.O. | Shevtsov, A.N. | Gurina, D.V., National Academy of Sciences. Scientific and Practical Center of Animal Breeding (Belarus) | Zhalnerovskaya, A.V., Vitebsk State Academy of Veterinary Medicine (Belarus)
In the conditions of the Republic of Belarus there was analyzed the productivity of 13-18-month old young stock of cattle which was bred for meat with various levels of energy value nutrition. Also, there were noted their slaughter qualities and chemical composition of meat. Administration of diet rations with an increased level of exchange energy and the best indices of protein fissility rendered a positive influence on productivity of young stock of fattening cattle and made it possible to obtain 1103-1100 gram of body weight gain. As a result, the energy value gain was 22,9-22,6 MJ along with conversion of metabolizable energy at a gain - 24,7-25,3%. Consumption of metabolizable energy per 1 MJ along with body weight gain were within 4,7-5,0 MJ. Feeding of rations with various levels of the studied factor rendered the positive influence on slaughter qualities, made it possible obtain slaughter yield of 53,6-54,6% at an optimum ratio of nutrients in meat. Feeding of bull calves with diets with excessive content (up to 10%) of metabolizable energy made it possible to lower the prime cost of obtained breeding products on 0,55%.
显示更多 [+] 显示较少 [-]Ветеринарно-санитарные показатели мяса кур-несушек при использовании в рационе ферментных препаратов
2010
Shulga, L.V. | Pakhomov, P.I., Vitebsk State Academy of Veterinary Medicine (Belarus)
In the conditions of the Republic of Belarus there was analyzed the efficiency of application multi-enzyme preparations (drugs) Ehkozim (contained beta-glucanases; endo-xilanase; cellulose) and Vitazim (contained complex of enzymatic carbohydrases: xylanase; cellulose; beta-glucanases) in diets of lying hens. Vitazim was applied for destruction of complex structures: dietary fibre, protein, starch. Ehkozim was applied for albuminolysis of the main nonstarch polysaccharides of fodders. It was established that application of preparations in feeding of lying hens promoted the increasing of biological value of poultry meat, and prove to be economically expedient. Poultry meat of analyzed samples in accordance with physical, chemical and bacteriological indices, as well as in accordance with biological value and harmlessness was not worse than meat of control samples. As a result of the realized bacteriological research of all experimental samples of meat and internal organs there were not stated microorganisms Е. coli, S. aureus, bacteria of sort Proteus, B. сеreus and sulfate-reducing clostridium, salmonellas.
显示更多 [+] 显示较少 [-]Интерьерные особенности утят при межлинейной гибридизации
2010
Petrukovich, T.V. | Ryskova, I.P., Vitebsk State Academy of Veterinary Medicine (Belarus)
In the conditions of the Republic of Belarus there was studied the possibility of increasing of meat yield productivity of ducklings of Temp cross in the conditions application of parental lines of Peking ducks of German breeding Stolle Seddin Vital (SVV) cross. In course of the study there was realized a comparative evaluation of linear and crossbred ducklings in accordance with meat qualities and blood indexes. There were presented the results of morphological composition of liner and crossbred duck carcasses; dynamics of changes of lipid exchange (total lipids; cholesterine; triglyceride) of ducklings; morphological composition of carcasses of father and mother combinations; blood indexes of ducklings; blood indexes of crossbred ducklings of final combinations. Research results showed that crossbred ducklings had high meat properties which exceeded the purebred ones. In ducklings with lower indexes of fattiness of carcasses there was noted the lowering of concentration of total lipids, cholesterine and triglyceride in blood.
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