Objective: This study aimed to develop a method for predicting the body weight of beef cattle using meta-analysis based on digital image processing. Materials and Methods: The meta-analysis process commenced by collecting studies with the keywords “beef cattle,” “correlation,” “digital image,” and “body weight” from Google Scholar and Science Direct. The obtained studies were reviewed papers based on their titles, abstracts, and content, and then categorized by authors, year, country, sample size, and correlation coefficient. A digital image of body measurements used included wither and hip height, chest depth, heart girth, body length, and top view. The statistical analysis was conducted by calculating effect sizes using the correlation coefficient and sample sizes. Results: The results of the meta-analysis, based on 3,017 cattle from 13 selected studies, showed the highest and lowest correlation coefficients for the top view variable and hip height. Based on cattle breed, significant differences (p < 0.05) were observed in the wither height variable with correlation coefficients of 0.94, 0.79, and 0.66 for Hanwoo, Holstein, and Simmental, respectively. Based on sex, significant differences (p < 0.05) were seen in the wither height variable, with correlation coefficients of 0.73 for males and 0.90 for females, while for hip height, the values were 0.70 and 0.87, respectively. Conclusion: In conclusion, to achieve the best accuracy in predicting the body weight of beef cattle based on a digital image, the top view variable can be used. However, for ease of field experimentation, body length or chest depth can also be used while taking breed and sex categories into the model. Adv. Vet. Anim. Res., 11(1): 153-160, March 2024 http://doi.org/10.5455/javar.2024.k760

Objective: This study aimed to develop a method for predicting the body weight of beef cattle using meta-analysis based on digital image processing. Materials and Methods: The meta-analysis process commenced by collecting studies with the keywords "beef cattle," "correlation," "digital image," and "body weight" from Google Scholar and Science Direct. The obtained studies were reviewed papers based on their titles, abstracts, and content, and then categorized by authors, year, country, sample size, and correlation coefficient. A digital image of body measurements used included wither and hip height, chest depth, heart girth, body length, and top view. The statistical analysis was conducted by calculating effect sizes using the correlation coefficient and sample sizes. Results: The results of the meta-analysis, based on 3,017 cattle from 13 selected studies, showed the highest and lowest correlation coefficients for the top view variable and hip height. Based on cattle breed, significant differences (p < 0.05) were observed in the wither height variable with correlation coefficients of 0.94, 0.79, and 0.66 for Hanwoo, Holstein, and Simmental, respectively. Based on sex, significant differences (p < 0.05) were seen in the wither height variable, with correlation coefficients of 0.73 for males and 0.90 for females, while for hip height, the values were 0.70 and 0.87, respectively. Conclusion: In conclusion, to achieve the best accuracy in predicting the body weight of beef cattle based on a digital image, the top view variable can be used. However, for ease of field experimentation, body length or chest depth can also be used while taking breed and sex categories into the model. [J Adv Vet Anim Res 2024; 11(1.000): 153-160]

Objective: This research assesses the utilization of sieved olive pulp (SOP) in the diet of growing rabbits through the use of an exogenous enzyme or dry yeast as a pretreatment. Materials and Methods: One hundred sixty-eight male V-Line rabbits aged 5 weeks (weighing 550 ± 25 gm) were randomly divided into seven groups with eight replicates each as follows: the control group was fed a basal diet without supplementation; while the other six groups were fed basal diets containing 20% and 25% of SOP and supplemented with 0.1 gm/kg Econase (E), 0.5 gm/kg dry yeast (Y), and a combination of both. The experiment lasted for 8 weeks. Results: The results indicated that supplementation of E, Y, and EY into rabbit diets containing SOP improved live body weight, body weight gain, feed conversion ratio, and nutrient digestibility. A higher dressing percentage was observed in the groups fed a 20% and 25% SOP diet supplemented with EY (p < 0.05). The treated groups showed an increase in total protein, albumin, globulin, A/G ratio, total antioxidant capacity, superoxide dismutase, and glutathione peroxidase (p < 0.05), while there was a significant decrease in triglycerides, total cholesterol, and malondialdehyde levels (p < 0.05) compared to the control. Rabbit groups fed an SOP diet supplemented with E, Y, or EY demonstrated higher (p < 0.05) economic efficiency compared to the control. Conclusion: Supplementing the diet of rabbits containing SOP with exogenous enzymes and/or dry yeast enhances the nutritional value of SOP while improving rabbit performance, nutrient digestibility, and antioxidant status. Adv. Vet. Anim. Res., 11(1): 161-170, March 2024 http://doi.org/10.5455/javar.2024.k761

Objective: This research assesses the utilization of sieved olive pulp (SOP) in the diet of growing rabbits through the use of an exogenous enzyme or dry yeast as a pretreatment. Materials and Methods: One hundred sixty-eight male V-Line rabbits aged 5 weeks (weighing 550 ± 25 gm) were randomly divided into seven groups with eight replicates each as follows: the control group was fed a basal diet without supplementation; while the other six groups were fed basal diets containing 20% and 25% of SOP and supplemented with 0.1 gm/kg Econase (E), 0.5 gm/kg dry yeast (Y), and a combination of both. The experiment lasted for 8 weeks. Results: The results indicated that supplementation of E, Y, and EY into rabbit diets containing SOP improved live body weight, body weight gain, feed conversion ratio, and nutrient digestibil¬ity. A higher dressing percentage was observed in the groups fed a 20% and 25% SOP diet sup¬plemented with EY (p < 0.05). The treated groups showed an increase in total protein, albumin, globulin, A/G ratio, total antioxidant capacity, superoxide dismutase, and glutathione peroxidase (p < 0.05), while there was a significant decrease in triglycerides, total cholesterol, and malondi¬aldehyde levels (p < 0.05) compared to the control. Rabbit groups fed an SOP diet supplemented with E, Y, or EY demonstrated higher (p < 0.05) economic efficiency compared to the control. Conclusion: Supplementing the diet of rabbits containing SOP with exogenous enzymes and/or dry yeast enhances the nutritional value of SOP while improving rabbit performance, nutrient digestibility, and antioxidant status. [J Adv Vet Anim Res 2024; 11(1.000): 161-170]

Objective: Many studies have observed different characteristics among productive systems in the rural territories of Latin America. Therefore, understanding and characterizing them while they function plays an essential role in determining their relationship between development and environment. A study has been conducted in the Orellana province of NE Ecuador to determine their typology and then classify them according to the variables that describe their main traits or attributes using cluster analysis (CA). Materials and Methods: A survey was structured to investigate physical, productive, environmental, as well as socioeconomic character variables, which were subsequently applied to a random sample of the 5,963 agricultural productive units (APUs) through face-to-face contact with producers during an in situ visit to their farms. Result: The CA allowed us to identify three typologies of APUs in the Orellana Province. The first has been Type 1, which is denominated as the most conventional (40%), while Type 2 uses more efficient natural resources but represents an amount of only 9.4%. In contrast, type 3 (50.6%) depends on a significant part of local or national development programs. Conclusion: All groups indicated some peculiarities in common, as there were marked differences in the use and distribution of land as well as production methods among them. Consequently, this pioneering study allowed us to identify different production methods. Therefore, we encourage local and national governments to establish policies for natural resource conservation in such high-diversity zones. Adv. Vet. Anim. Res., 11(1): 171-180, March 2024 http://doi.org/10.5455/javar.2024.k762

Objective: Many studies have observed different characteristics among productive systems in the rural territories of Latin America. Therefore, understanding and characterizing them while they function plays an essential role in determining their relationship between development and environment. A study has been conducted in the Orellana province of NE Ecuador to determine their typology and then classify them according to the variables that describe their main traits or attributes using cluster analysis (CA). Materials and Methods: A survey was structured to investigate physical, productive, environmental, as well as socioeconomic character variables, which were subsequently applied to a random sample of the 5,963 agricultural productive units (APUs) through face-to-face contact with producers during an in situ visit to their farms. Result: The CA allowed us to identify three typologies of APUs in the Orellana Province. The first has been Type 1, which is denominated as the most conventional (40%), while Type 2 uses more efficient natural resources but represents an amount of only 9.4%. In contrast, type 3 (50.6%) depends on a significant part of local or national development programs. Conclusion: All groups indicated some peculiarities in common, as there were marked differences in the use and distribution of land as well as production methods among them. Consequently, this pioneering study allowed us to identify different production methods. Therefore, we encourage local and national governments to establish policies for natural resource conservation in such high-diversity zones. [J Adv Vet Anim Res 2024; 11(1.000): 171-180]

Objective: Different sources and levels of starch in the total mixed ration might result in different rumen fermentation profiles, rumen microbial composition, and enteric methane production. The current study aimed to evaluate the effect of dietary starch intake on enteric methane production through a meta-analytical approach by integrating related published studies. Materials and Methods: Papers that provided study results on enteric methane production from cattle fed different sources and levels of starch were selected. A total of 52 publications were filtered based on some specified criteria, comprised of 73 studies focused on enteric methane production in cattle fed a basal diet supplemented with starch. The collected data were subjected to statistical meta-analysis through a mixed methodology model. The p-value and root mean square error (RMSE) were applied as the statistical models. Results: Results showed that increasing the level of dietary starch intake and its digestibility in the rumen as well as increasing propionate significantly decreased the acetate to propionate (A/P) ratio as well as enteric methane production (p < 0.01). Conclusions: It is concluded that the addition of starch to diets mitigates enteric methane production from cattle, primarily through two ways, i.e., manipulation of the protozoa population and alteration to a lower A/P ratio. Adv. Vet. Anim. Res., 11(1): 212-230, March 2024    http://doi.org/10.5455/javar.2024.k767

Objective: Different sources and levels of starch in the total mixed ration might result in different rumen fermentation profiles, rumen microbial composition, and enteric methane production. The current study aimed to evaluate the effect of dietary starch intake on enteric methane production through a meta-analytical approach by integrating related published studies. Materials and Methods: Papers that provided study results on enteric methane production from cattle fed different sources and levels of starch were selected. A total of 52 publications were filtered based on some specified criteria, comprised of 73 studies focused on enteric methane production in cattle fed a basal diet supplemented with starch. The collected data were subjected to statistical meta-analysis through a mixed methodology model. The p-value and root mean square error (RMSE) were applied as the statistical models. Results: Results showed that increasing the level of dietary starch intake and its digestibility in the rumen as well as increasing propionate significantly decreased the acetate to propionate (A/P)ratio as well as enteric methane production (p < 0.01). Conclusions: It is concluded that the addition of starch to diets mitigates enteric methane production from cattle, primarily through two ways, i.e., manipulation of the protozoa population and alteration to a lower A/P ratio. [J Adv Vet Anim Res 2024; 11(1.000): 212-230]

Objective: Infectious bronchitis (IB), a highly infectious acute viral disease, is a major burden to the chicken industry worldwide. The research aimed to develop an inactivated IB vaccine using local isolates and assess its immunogenicity compared to other commercial live IB vaccines. Materials and Methods: An inactivated vaccine using a candidate IB virus (PP067159.1: Alim_IB_1001) of the QX genotype was developed according to WOAH guidelines. Chickens were vaccinated with three doses (0.25, 0.5, and 1.0 ml) at 7 days old, with a booster at 37 days old via subcutaneous (SC) and intramuscular (IM) routes. Blood samples were collected on days 7, 37, and 67 to measure immune response by indirect ELISA. On day 67, chickens were challenged with a virulent IBV strain to assess vaccine protection. The experimental IB vaccine’s immunogenicity, protective efficacy, and antibody duration was compared to a live IB vaccine (Live CEVAC® IBird) using three vaccination schedules: killed-followed-killed, live-followed-killed, and live-followed-live. Results: Chickens vaccinated with SC with 1.0 ml showed higher antibody titers compared to other SC and IM routes of vaccination. SC vaccination with 0.5 and 1 ml provided the highest protection (93%). The killed-followed-killed vaccination method produced a more consistent and protective level of antibody titers in chickens compared to the other vaccination schedules. The experimental inactivated IB vaccine led to a higher survival rate (93%) compared to live-followed-killed (87%) and live-followed-live (73%), with statistical significance (p < 0.01). All three chicken groups maintained protective antibody titers (> 396) at 307 days, but titers declined faster in the live-followed-live and live-followed-killed groups compared to the killed-followed-killed group. Conclusion: The study found that the experimental inactivated IB vaccination can protect commercial-layer chickens from natural IB outbreaks of the QX genotype. J. Adv. Vet. Anim. Res., 11(3): 592–600, September 2024 http://doi.org/10.5455/javar.2024.k809

Objective: Infectious bronchitis (IB), a highly infectious acute viral disease, is a major burden to the chicken industry worldwide. The research aimed to develop an inactivated IB vaccine using local isolates and assess its immunogenicity compared to other commercial live IB vaccines. Materials and Methods: An inactivated vaccine using a candidate IB virus (PP067159.1: Alim_IB_1001) of the QX genotype was developed according to WOAH guidelines. Chickens were vaccinated with three doses (0.25, 0.5, and 1.0 ml) at 7 days old, with a booster at 37 days old via subcutaneous (SC) and intramuscular (IM) routes. Blood samples were collected on days 7, 37, and 67 to measure immune response by indirect ELISA. On day 67, chickens were challenged with a virulent IBV strain to assess vaccine protection. The experimental IB vaccine's immunogenicity, protective efficacy, and antibody duration were compared to a live IB vaccine (Live CEVAC® IBird) using three vaccination schedules: killed-followed-killed, live-followed-killed, and live-followed-live. Results: Chickens vaccinated with SC with 1.0 ml showed higher antibody titers compared to other SC and IM routes of vaccination. SC vaccination with 0.5 and 1 ml provided the highest protection (93%). The killed-followed-killed vaccination method produced a more consistent and protective level of antibody titers in chickens compared to the other vaccination schedules. The experimental inactivated IB vaccine led to a higher survival rate (93%) compared to live-followed-killed (87%) and live-followed-live (73%), with statistical significance (p < 0.01). All three chicken groups maintained protective antibody titers (>396) at 307 days, but titers declined faster in the live-followed-live and live-followed-killed groups compared to the killed-followed-killed group. Conclusion: The study found that the experimental inactivated IB vaccination can protect com¬mercial-layer chickens from natural IB outbreaks of the QX genotype. [J Adv Vet Anim Res 2024; 11(3.000): 592-600]

Objective: The purpose of this study was to characterize the circulating Peste des Petits Ruminants virus (PPRV) from slaughtered goats and conduct a phylogenetic analysis of the N gene of PPRV. Materials and Methods: A total of 196 slaughtered goats were investigated at the marketplaces of Mymensingh division from January 2019 to March 2021. Lungs, spleen, and mesenteric lymph nodes were collected for histology and molecular study. In-house developed Reverse-Transcriptase-Polymerase Chain Reaction (RT-PCR) protocols were carried out using designed primer sets (PPRV NF-gctctgtgattgcggctgagc and PPRV NR-cctggtcctccagaatcttggcc). The CLC sequence viewer was used for phylogenetic analysis. Results: Grossly pneumonic lungs, shrinkage spleen, and enlarged mesenteric lymph nodes with hemorrhages were recorded. Both intracytoplasmic and intranuclear inclusion bodies were seen in lymphocytes of the mesenteric lymph node, spleen, and lungs. PPRV was detected in 37 goats (18.9%) by RT-PCR test. The 402-bp amplicon was generated in PPRV-positive cases. The phylogenetic analysis showed that the studied PPRV isolates of the Mymensingh division belonged to lineage IV. Conclusion: The prevalence of PPR was 18.9% in slaughtered goats at marketplaces in the Mymensingh division. Slaughterhouses may be a source of PPRV, and it can be horizontally transmitted from the meat market to the farm. Restricting sick animal movement within the country, mass PPR vaccination campaigns, increased awareness, and improved biosecurity in the meat market may lessen the incidence of PPR in goats. J. Adv. Vet. Anim. Res., 11(3): 655–663, September 2024 http://doi.org/10.5455/javar.2024.k815

Objective: The purpose of this study was to characterize the circulating Peste des Petits Ruminants virus (PPRV) from slaughtered goats and conduct a phylogenetic analysis of the N gene of PPRV. Materials and Methods: A total of 196 slaughtered goats were investigated at the marketplaces of Mymensingh division from January 2019 to March 2021. Lungs, spleen, and mesenteric lymph nodes were collected for histology and molecular study. In-house developed Reverse- Transcriptase-Polymerase Chain Reaction (RT-PCR) protocols were carried out using designed primer sets (PPRV NF-gctctgtgattgcggctgagc and PPRV NR-cctggtcctccagaatcttggcc). The CLC sequence viewer was used for phylogenetic analysis. Results: Grossly pneumonic lungs, shrinkage spleen, and enlarged mesenteric lymph nodes with hemorrhages were recorded. Both intracytoplasmic and intranuclear inclusion bodies were seen in lymphocytes of the mesenteric lymph node, spleen, and lungs. PPRV was detected in 37 goats (18.9%) by RT-PCR test. The 402-bp amplicon was generated in PPRV-positive cases. The phylo¬genetic analysis showed that the studied PPRV isolates of the Mymensingh division belonged to lineage IV. Conclusion: The prevalence of PPR was 18.9% in slaughtered goats at marketplaces in the Mymensingh division. Slaughterhouses may be a source of PPRV, and it can be horizontally trans¬mitted from the meat market to the farm. Restricting sick animal movement within the country, mass PPR vaccination campaigns, increased awareness, and improved biosecurity in the meat market may lessen the incidence of PPR in goats. [J Adv Vet Anim Res 2024; 11(3.000): 655-663]

Objective: The current study evaluated the greenhouse gas (GHG) emissions of dairy cattle through the Global Livestock Environmental Assessment Model (GLEAM) model and illustrated potential mitigation strategies by modifying nutrition interventions. Materials and Methods: A semi-structural questionnaire was developed to calculate dairy animal GHG emissions. This study comprised 40 farmers from four districts: river basin (Pabna), drought prone (Chapainobabganj), floodplain (Nilphamari), and saline-prone (Sathkhira) areas. Ten lactating cows (two cows from each farmer) were also selected to collect information on feeding practices, feed resources, feed intake (roughages and concentrate), water intake, and productive and reproductive parameters for 7 days at each site during two seasons: dry (November– February) and wet (June–October). Results: The GHG emissions from the river basin area were significantly (p < 0.05) higher due to low-quality roughages (75%), whereas CH4 /kg of milk production was the lowest (77.0 gm). In contrast, the area that frequently experiences drought showed a different pattern. For instance, the generation of CH4 from enteric fermentation was 1187.4 tons/year, while the production of CH4 and N2O from manure management was 323.1 tons/year and 4.86 tons/year, respectively. In comparison to other climatic areas, these values were the lowest because the supply of green grass was twice as abundant as in the other climatic areas (40%). The quantity of CH4 /kg of milk produced in an area susceptible to drought did not vary. Conclusion: Implementing feeding systems in drought-prone areas is a successful approach to reducing GHG emissions in the dairy industry in Bangladesh. Consequently, implementing feed-balancing techniques can enhance productivity and foster environmentally sustainable animal production. J. Adv. Vet. Anim. Res., 11(3): 664–674, September 2024http://doi.org/10.5455/javar.2024.k816

Objective: The current study evaluated the greenhouse gas (GHG) emissions of dairy cattle through the Global Livestock Environmental Assessment Model (GLEAM) model and illustrated potential mitigation strategies by modifying nutrition interventions. Materials and Methods: A semi-structural questionnaire was developed to calculate dairy animal GHG emissions. This study comprised 40 farmers from four districts: river basin (Pabna), drought-prone (Chapainobabganj), floodplain (Nilphamari), and saline-prone (Sathkhira) areas. Ten lac¬tating cows (two cows from each farmer) were also selected to collect information on feeding practices, feed resources, feed intake (roughages and concentrate), water intake, and production and reproductive parameters for 7 days at each site during two seasons: dry (November– February) and wet (June–October). Results: The GHG emissions from the river basin area were significantly (p < 0.05) higher due to low-quality roughages (75%), whereas CH4/kg of milk production was the lowest (77.0 gm). In contrast, the area that frequently experiences drought showed a different pattern. For instance, the generation of CH4 from enteric fermentation was 1187.4 tons/year, while the production of CH4 and N2O from manure management was 323.1 tons/year and 4.86 tons/year, respectively. In comparison to other climatic areas, these values were the lowest because the supply of green grass was twice as abundant as in the other climatic areas (40%). The quantity of CH4/kg of milk produced in an area susceptible to drought did not vary. Conclusion: Implementing feeding systems in drought-prone areas is a successful approach to reducing GHG emissions in the dairy industry in Bangladesh. Consequently, implementing feed-balancing techniques can enhance productivity and foster environmentally sustainable ani¬mal production. [J Adv Vet Anim Res 2024; 11(3.000): 664-674]

Objectives: This study aimed to investigate the physical properties of Indonesian local sheep skin collagen extracted by acid and enzymatic methods. Materials and Methods: Collagen was isolated from Pure Breed Garut Sheep (Ovis aries sp.) skin, 1.5 years old. The skins were obtained from a local slaughterhouse in Cirebon, Indonesia. The solvents used were CH3COOH and three different enzymes: neutrase, alcalase, and bromelain. Results: The highest yields of extracted collagen were bromelain-soluble collagen (BSC), which reached 37.07%. The range of Ph values for all samples started from 4.01 to 4.76. The viscosity values (cP) of acid-soluble collagen (ASC), neutrase-soluble collagen (NSC), alcalase-soluble collagen (LSC), and BSC were 3.42, 3.90, 3.45, and 3.12, respectively. Regarding SDS-PAGE analyses, Garut sheepskin collagen is categorized as collagen type I, which has a molecular weight of about 140.99 to 148.74 kDa for α1 and around 110 to 111.86 kDa for α2. The results of FTIR and DSC analyses for all samples show the same motif with commercial collagen motifs based on the literature. Conclusion: Garut sheep skin has the potential to be an alternative raw material source for producing collagen. Collagen extracted using a combination of CH3COOH and bromelain enzyme showed the most desirable results in almost every characteristic. J. Adv. Vet. Anim. Res., 11(3): 722–728, September 2024 http://doi.org/10.5455/javar.2024.k823

Objectives: This study aimed to investigate the physical properties of Indonesian local sheep skin collagen extracted by acid and enzymatic methods. Materials and Methods: Collagen was isolated from Pure Breed Garut Sheep (Ovis aries sp.) skin, 1.5 years old. The skins were obtained from a local slaughterhouse in Cirebon, Indonesia. The solvents used were CH3COOH and three different enzymes: neutrase, alcalase, and bromelain. Results: The highest yields of extracted collagen were bromelain-soluble collagen (BSC), which reached 37.07%. The range of Ph values for all samples started from 4.01 to 4.76. The viscosity values (cP) of acid-soluble collagen (ASC), neutrase-soluble collagen (NSC), alcalase-soluble collagen (LSC), and BSC were 3.42, 3.90, 3.45, and 3.12, respectively. Regarding SDS-PAGE analyses, Garut sheepskin collagen is categorized as collagen type I, which has a molecular weight of about 140.99 to 148.74 kDa for α1 and around 110 to 111.86 kDa for α2. The results of FTIR and DSC analyses for all samples show the same motif with commercial collagen motifs based on the literature. Conclusion: Garut sheep skin has the potential to be an alternative raw material source for producing collagen. Collagen extracted using a combination of CH3COOH and bromelain enzyme showed the most desirable results in almost every characteristic. [J Adv Vet Anim Res 2024; 11(3.000): 722-728]

Objective: Mastitis is the most common and costly dairy cow disease worldwide. We performed an intensive analysis of mastitis prevalence, pathogens, and treatments using retrospective data from a commercial dairy farm in Germany to estimate the severity of mastitis in the commercial production system and to give on-farm insights. Material and Methods: Milking system data and cow-individual data were collected over 9 years (2012-2021). A resilient amount of data from 1537 cows, >1,000 mastitis infections, 1901 pathogens, and 5729 treatments have been analyzed. Results: Mastitis occurrence was highest in summer (45.0%), in first lactation (51.1%), and in the late lactation stage (36.7%). The relative mastitis frequency increased sharply with a high lactation number (>7). The leading pathogens causing mastitis were coagulase-negative staphylococci (28.3%). Approximately 25% of mastitis cases were treated with non-antibiotic medicine and 75% with antibiotics. For the latter, cephalosporins and aminoglycosides were the most administered. The average mastitis treatment duration was 3.48 days. During the study time, the farm changed from a conventional milking system to an automatic milking system in 2015, which has not negatively affected the number of recorded mastitis infections. Conclusion: This case report gives detailed insights about mastitis incidences gained under practical conditions. Novel information about mastitis drug usage and duration is presented. Potential mastitis risk factors identified from the results of this study were the summer season, first or >7 lactation(s), and the late lactation stage. J. Adv. Vet. Anim. Res., 11(4): 856–868, December 2024 http://doi.org/10.5455/javar.2024.k837

Objective: Mastitis is the most common and costly dairy cow disease worldwide. We performed an intensive analysis of mastitis prevalence, pathogens, and treatments using retrospective data from a commercial dairy farm in Germany to estimate the severity of mastitis in the commercial production system and to give on-farm insights. Material and Methods: Milking system data and cow-individual data were collected over 9 years (2012-2021). A resilient amount of data from 1537 cows, >1,000 mastitis infections, 1901 patho¬gens, and 5729 treatments have been analyzed. Results: Mastitis occurrence was highest in summer (45.0%), in first lactation (51.1%), and in the late lactation stage (36.7%). The relative mastitis frequency increased sharply with a high lacta¬tion number (>7). The leading pathogens causing mastitis were coagulase-negative staphylococci (28.3%). Approximately 25% of mastitis cases were treated with non-antibiotic medicine and 75% with antibiotics. For the latter, cephalosporins and aminoglycosides were the most administered. The average mastitis treatment duration was 3.48 days. During the study time, the farm changed from a conventional milking system to an automatic milking system in 2015, which has not nega¬tively affected the number of recorded mastitis infections. Conclusion: This case report gives detailed insights about mastitis incidences gained under practical conditions. Novel information about mastitis drug usage and duration is presented. Potential mastitis risk factors identified from the results of this study were the summer season, first or >7 lactation(s), and the late lactation stage. [J Adv Vet Anim Res 2024; 11(4.000): 856-868]