Diclofenac was responsible for the decimation of Gyps vulture species on the Indian subcontinent during the 1980s and 1990s. Gyps vultures are extremely sensitive (the lethal dose 50 [LD50] ~ 0.1 mg/kg – 0.2 mg/kg), with toxicity appearing to be linked to metabolic deficiency, demonstrated by the long T1/2 (~12 h – 17 h). This is in striking comparison to the domestic chicken (Gallus gallus domesticus), in which the LD50 is ~10 mg/kg and the T1/2 is ~1 h. The phase 1 cytochrome P450 (CYP) 2C subfamily has been cited as a possible reason for metabolic deficiency. The aim of this study was to determine if CYP2C9 homolog pharmacogenomic differences amongst avian species is driving diclofenac toxicity in Gyps vultures. We exposed each of 10 CYP-inhibited test group chickens to a unique dose of diclofenac (as per the Organisation for Economic Co-operation and Development [OECD] toxicity testing guidelines) and compared the toxicity and pharmacokinetic results to control group birds that received no CYP inhibitor. Although no differences were noted in the LD50 values for each group (11.92 mg/kg in the CYP-inhibited test group and 11.58 mg/kg in the control group), the pharmacokinetic profile of the test group was suggestive of partial inhibition of CYP metabolism. Evaluation of the metabolite peaks produced also suggested partial metabolic inhibition in test group birds, as they produced lower amounts of metabolites for one of the three peaks demonstrated and had higher diclofenac exposure. This pilot study supports the hypothesis that CYP metabolism is varied amongst bird species and may explain the higher resilience to diclofenac in the chicken versus vultures.

This study was carried out on a total of 550 lactating animals; 310 and 240 cows and buffaloes, respectively which were examined for signs of clinical mastitis (swelling, hotness, redness, and apparent milk change) from different dairy farms and veterinary units located at El-Fayoum Governorate during the period from May 2017 to November 2017. Clinical examination proved that out of these animals, a total of 126 animals (87 cattle and 39 buffaloes) were found with clinical mastitis. Streptococcus species were recovered from 73 animals including; 29(39.7%) and 44(60.0%) cows and buffaloes, respectively. Furthermore, out of the 73 Streptococci isolates recovered from cows and buffaloes; there were 10(13.7%) and 15(20.5%) S. agalactiae, 5(6.8%) and 10(23.7%) S. dysgalactiae, 8 (10.6%) and 7 (13.7%) S. uberis, 3(4.1%) and 10(13.7 %) E. fecalis and 3(4.1%) and 2(2.7%) S. lactarius, respectively. Anti-microbial susceptibility testing showed that the highest resistance was recorded against penicillin, gentamicin, streptomycin, and doxycycline (100%). Conversely, the highest sensitivity was recorded against ceftriaxone, ciprofloxacin, and sulfamethoxazole-trimethoprim (100%). Biofilm formation capacity was phenotypically assessed on YESCA CR agar medium and showed that all examined S. agalactiae and S. dysgalactiae were strong biofilm producers, meanwhile, 78%, 50%, and 75% of S. uberis, S. lactarius, and E. fecalis were biofilm positive isolates respectively. Application of PCR technique revealed that enterotoxins producing genes; sed, seb were found in 20% and 80% of isolates, in order. Biofilm-associated genes; fnbA and icaA genes were detected in 90% and 70%, respectively. Resistance genes; mecA and blaZ, genes were possessed in 90% and 70% of isolates, respectively.

Detailed information on specific species of non-aureus staphylococci (NAS) has become a necessity for effective udder health control programs in South Africa. The main objective of this preliminary study was to identify the different NAS species and strains present in dairy herds in South Africa using a cost-effective method. A further objective was to investigate the effects of cow risk factors and farming systems on the NAS isolates identified. A total of 214 NAS, isolated from milk collected from 17 South African dairy herds, were identified using three diagnostic tests (API Staph test, MALDI-TOF and 16s rRNA). There was a good observed agreement between the MALDI-TOF and 16S rRNA sequencing (92.2%) and a poor observed agreement between the MALDI-TOF and API Staph (25.7%). The genetic relatedness within species was investigated in 128 of these isolates using random polymorphic amplified deoxyribonucleic acid (DNA) (RAPD), verified by multilocus sequence typing (MLST), and phylogenetic analysis and cow risk factors were investigated on species level. The main NAS species isolated were Staphylococcus chromogenes (75.2%), Staphylococcus epidermidis (9.4%) and Staphylococcus haemolyticus (8.9%). The RAPD test identified 34 Staphylococcus chromogenes, 13 Staphylococcus epidermidis and nine Staphylococcus haemolyticus strains, indicating genetic diversity amongst strains and herds. The presence of NAS intramammary infections was found to be significantly related to the farming systems, composite cow milk somatic cell count (SCC), parity and days in milk (DIM). Significantly more NAS were isolated from primiparous and from older cows. This knowledge could assist with the management of NAS on dairy farms.

This study was designed to determine the prevalence of enterotoxigenic S. aureus in table eggs in El-Fayoum city, Egypt. A total of 250 table egg samples (75 Baladi hens’, 75 white farm hens’, 75 brown farm hens’ and 25 duck egg samples) were collected randomly from poultry farms, groceries, supermarkets, and street vendors in El-Fayoum city, Egypt. Each Baladi hen ҆s egg sample was represented by five eggs, while each farm hen ҆s and duck egg sample was represented by three eggs. The shells and contents of eggs were examined for the presence of Staphylococcus spp < /em>., coagulase (coa), and staphylococcal enterotoxins (Ses) genes. The obtained results revealed that the examined samples of shells and contents of Baladi hens ҆, poultry farms ҆ (white and brown), and ducks ҆ eggs were contaminated with Staphylococcus spp. with incidences of 24.0, 9.3, 5.3, 44.0, 8.0, 2.7, 1.3 and 12.0 %, respectively and coagulase-positive S. aureus with the incidences of 16.7, 14.3, 0.0, 18.2, 0.0, 0.0, 0.0 and 33.3 %, respectively. Enterotoxin profiling by PCR proved that two classical enterotoxin genes (Seb and Sed) were produced from three (42.86%) coagulase-positive S. aureus strains, as two Baladi hens’ ҆ eggshells produced Seb and one of the ducks ҆ egg contents produced Sed. The public health hazards of the isolated strains and enterotoxins had been discussed.

The knowledge of the proboscis morphology is of a particular significance, especially in the field of comparative veterinary anatomy and rhinology. The proboscis is a specialized nasal organ, which has several anatomical modifications that enable the animal to withstand the adverse climatic conditions. Remarkably, the anatomy of the camel nose is distinct in comparison with ruminant and other domestic animals. This has been attributed to the essential proboscideal criteria to which the camel nose possesses. However, little is known about the proboscis-like structure of the dromedarian nose. Therefore, the aim of the study was to elucidate the idea that camel nose is best interpreted as a sort of proboscis.

Feline parvovirus infection (FPV) is one of the serious diseases in Kittens that causes substantial morbidity and death. For the treatment of affected cats and the prevention of disease spread, early diagnosis of FPV infection is critical. To our knowledge, there have been no reports about the disease’s situation in Egypt’s Assiut province. As a result, the goal of this study was to find out how common FPV infection is among ill cats in this province. A total of 30 cats suspected of being infected with FPV were screened using an antigen rapid test to determine whether they were clinically suspicious. To determine the prevalence of FPV, each investigated cat’s age, sex, breed, season, lifestyle (whether kept indoors or outdoors), and immunization were all documented. Overall, 26.7% of examined cats were affected. FPV infection was more common in young, unvaccinated cats who lived outdoor. Epizootiological monitoring of the prevalence rate based on cat breeds and sex revealed no statistically significant differences. In terms of season, spring had the highest infection rate (57.1%), followed by winter (33.3%), and autumn (7.69%). The rapid one-step test is a useful diagnostic tool for detecting FPV, which was found in the research area’s cats.

The objective of the present study was to compare between three anesthetic protocols for long-term anesthesia (2 h); protocol 1: xylazine (1mg/kg)/ketamine (10mg/kg) and inhalation of isoflurane in 100% of oxygenprotocol 2: xylazine (1mg/kg)/ketamine (10mg/kg) and propofol (24mg/kg/h) by CRI, and protocol 3: propofol (5mg/kg) and propofol (12mg/kg/h) by CRI. Three clinically healthy adult mongrel dogs of both sexes were used. Food and water were withheld for 12 and 6 h before induction of anesthesia, respectively. Intravenous catheter was placed in the cephalic vein. A wash-out period of 15 days was allowed between protocols. For all protocols (1, 2, and 3), physiological parameters were recorded using a patient monitor. Depth of anesthesia and muscle relaxation were determined by recording various reflexes. Times of first limb movement, regaining swallowing reflex and extubation, sternal recumbency, and standing were recorded. Quality of recovery was also recorded. Venous blood samples were collected into EDTA-containing Vacutainer tubes immediately before anesthesia, 10min after induction with ketamine/propofol, one hour and two h of anesthesia and after complete recovery for hematological analysis by using an automated machine. Data were recorded and expressed as mean ± SD and analyzed with commercial statistical software. Results revealed slight variations among animals of the three protocols in the physiological parameters. The quality and depth of anesthesia were excellent in dogs anesthetized with isoflurane and were good in dogs during propofol infusion. The duration of deep anesthesia during propofol infusion was shorter than isoflurane, with the shortest duration in protocol 3. There were variations among animals of the three protocols in the recovery parameters. The mean scores of the recovery quality were 4.3, 4.7, and 4 for protocols 1, 2, and 3, respectively, which sited between good and excellent scores of recoveries. Slight ataxia was recorded in a dog of protocol 1. However, ataxia and urination were recorded in dogs of protocols 1 and 2. In conclusion, the effect of isoflurane and propofol on physiological parameters of dogs during long-term anesthesia was minimum and recovery was uneventful. The quality and depth of anesthesia were excellent in dogs anesthetized with isoflurane and were good in dogs during propofol infusion. The duration of deep anesthesia during propofol infusion was shorter than isoflurane, with the shortest duration in protocol 3 (12mg/kg/h). Isoflurane provides more reliable and consistent anesthetic plane plus it's not expensive as propofol. However, it requires special equipment. Propofol achieved effective anesthesia, with fast induction and less hypothermia than isoflurane administration. Apnea was recorded in two dogs after initial induction with propofol.

The anatomical description of the beak, oropharyngeal and nasal cavities of broad-breasted white turkey (BBWT) is unavailable in comparison with other birds. This study was conducted on five adult healthy birds (16.63 ± 5.54 Kg bwt). Beak was boat-shaped with pointed apex and was hard and rigid horny structure.  The oral roof (palate) was formed of one median, two rostral, and two caudal palatine ridges. Shallow wide groove was observed between the rostral palatine ridges and lateral edges of the upper beak. The choanal slit was occupied 47.5% of the palate. There were two types of mechanical papillae distributed around the choanal slit. This slit was separated from the infundibular slit by a shallow groove. The tongue was triangular-shaped with pointed apex accompanied by V-shaped row of caudally directed lingual papillae between the body and the root. The lingual root was triangular-shaped, marked by shallow crescentic-shaped groove. The laryngeal mound was elongated pyramidal-shaped, contained glottis continued caudally by shallow longitudinal groove termed laryngeal sulcus. The nasal cavity was divided by a cartilaginous nasal septum into two symmetrical haves and opened externally by an elliptical-shaped nostril. The nostril was covered with a cartilaginous operculum which appeared as dome-shaped. Three nasal conchae were noticed within the nasal cavity: rostral, middle, and caudal nasal conchae. The middle nasal concha was the largest one, scrolled into 11/2 turns rostrally and scrolled 2 turns caudally. In conclusion, this study provides sufficient data on the anatomic and morphometric investigations of the nasal and oropharyngeal cavities of broad breast white turkey which may beneficial for manipulation of head during treatment of diseases.

Normal lactation records of 1717 Holstein cows belong to commercial dairy farm covered a period of 11 years from 1995 to 2005 were used in this study to evaluate the effects of calving season and parity on reproductive performance under subtropical desert conditions. The reproductive traits studied were age at first calving (AFC, month), first service period (FSP, day), number of services per conception (NSC, number), days open (DO, day), calving interval (CI, month), projected minimum calving interval (PMCI, month), breeding interval (BI, day) and conception rate (CR, %). NSC, DO, CI, and PMCI were enhanced significantly in winter compared to summer. Also, PMCI value in winter was lower compared to summer. The overall means of FSP, DO, PMCI, and BI were lower in multiparous cows compared to those in primiparous. No influences of interaction between season of calving and parity were detected on the reproductive traits of FSP, NSC, DO, CI and PMCI. CR had been influenced by season of calving as cows calved in winter exhibited higher CR (44%) than those calved in summer (39%). Heritability estimate of NSC, DO, CI and PMCI heritabilities were low and ranged from 0.07 to 0.19. In contrast, FSP and BI had medium heritabilities. Genetic correlations were low and positive between each of NSC, FSP or BI, between CI and each of NSC or FSP. In contrast, Genetic correlations were medium to high positive among other traits. The phenotypic correlations between NSC and each of FSP or BI were negatively low. Other phenotypic correlations were mostly similar in value and direction as the corresponding genetic correlations. Genetic trends of FSP, DO, CI and PMCI exhibited clear deterioration by time. They tended to decrease throughout the years of study. In contrast NSC and BI breeding values tended to increase during the same period.

Schmallenberg virus (SBV) is an emerging arthropod-borne pathogen of domestic and wild ruminants responsible for devasting economic losses in European countries with a potential for spreading across boundaries. Three hundred sixty-two (362) serum samples were randomly collected from 7 selected sheep and goat farms from January to October 2019 to investigate the seroprevalence of SBV among small ruminants in Negeri Sembilan and Terengganu states. Seroprevalence of SBV was determined using commercial ID vet® SBV multispecies cELISA test. The farmers completed a structured questionnaire to furnish farm management data to evaluate the risk factors associated with the seroprevalence of SBV among individual animals. A Chi-square test was performed to determine the association between seroprevalence of SBV and putative risk factors of individual animals. Based on the ELISA test, a total of 78 individual animals were seropositive with an overall prevalence of 21.5% and the true prevalence of 22.1% (95% CI:18.06-26.71). Multivariable logistic regression analysis further revealed that Negeri Sembilan state (p<0.001; OR=14.290; 95% CI=5.384-37.930), Breed: Malin sheep (p<0.001; OR=20.453; 95% CI=4.807-87.022), Barbados Black Belly sheep (p<0.01; OR=69.947; 95% CI=16.819-290.901), Boer goat (p<0.001; OR=9.108; 95% CI=3.191-25.997) and crossbred goat (p=0.001; OR=4.964; 95% CI=1.905-12.934) are risk factors of SBV among individual animals. This study highlights the prevalence of a new transboundary disease among small ruminants in Malaysia. The result obtained here provides preliminary data that could guide livestock producers and policy makers in designing suitable prevention and control measures.