To validate the physiological properties of the histamine receptors of ileal smooth muscle in dog, the effect of adrenergic, cholineric and H-receptor antagonists on the responses of ileal smooth muscle strips to histamine were investigated. Histamine caused the contraction of ileal smooth muscle and the contractile responses increased between the concentration of histamine (10-7M and 10-5 M) with dosedependent manner in dog. The shorter the treatment interval of histamine, the lower the contractile activity until the treatment interval extended to 40 minutes

To investigate the factors influencing the artificial transformation in Escherichia coli, E. coli C 600 was transformed by pBR322 DNA with tetracycline and ampicillin resistant gene purified by CsCL-EtBr equilibrium density gradient centrifugation from E. coli HB 101. The best conditions for the transformation of E. coli C 600 with pBR322 DNA were treatment with 100mM CaCl2, ice incubation at 0 deg C for 45 minutes, heat shock at 42 deg C for 4 min., 30 min. of ice incubation and incubation at 37 deg C for 100 min. for gene expression in that order

Gross and histological findings were obtained on cysts formed in the reproductive organs, particularly in the mesosalpinx, mesovarium, and fimbria, of 144 gilts and 37 sows. Of the 181 gilts and sows, 63 (34.8%) were found to have cysts which totaled 89. The number of cysts totaled 38 for the gilts and 51 for the sows, with relative frequencies of 18.1% 48.6%, respectively. The total number of cysts and the incidence were greater in the sows than in the gilts

In the evaluation of anthelmintic efficacy by fecal egg counts for cattle naturally infected with helminths, the reasonable technique of fecal egg counts and the reliable guidelines were determined. Modified technique of Harashigeru and Kim's sedimental tube was the most reasonable in fluke egg counts. Universal egg counting technique was preciser than McMaster egg counting technique and was lower in coefficient variation. Fecal egg counts of pretreatment should be carried out twice and mean of the egg should be calculated

To investigate the sensitivity of repeat tuberculin test with bovine PPD, seibert's fraction A(SFA) and avian PPD tuberculins in visible and non-visible lesion reactor cattle to HCSM tuberculin. The comparative test, under the condition of this experiment, was entirely unsatisfactory to differentiate between visible and non-visible lesion cases, the high frequency distribution of the skin fold thickness greater than 10mm to SFA tuberculin appeared in visible lesion cases, whereas the skin reaction 1 less than 4mm to the SFA tuberculin appeared in NVL cases

This study was undertaken to investigate congenital microphthalmia that occurred at a pig farm in Kyongbuk Korea [R.]. These microphthalmic piglets composed of 20 piglets farrowed by 3 different sows (1, 11 and 8 piglets by No. 1,2 and 3 sow, respectively) that were apparently normal during pregnancy and after parturition. Attempts were made to know epidemiological findings and histopathological changes of these piglets

The hemoglobin phenotype and the gene frequencies of 44 Formosan deer (Cervus nippon) in Kyunggido Korea [R.] area were examined by using cellulose acetate and starch gel electrophoresis. The method of cellulose acetate electrophoresis was simpler, more clear and preserative than starch gel electrophoresis. The hemoglobin phenotype had 3 types as (HbF) (HbFS and HbS). The frequencies of appearence were HbF 47.7% HbFS 47.7% and HbS 4.5%, respectively. The genetic factors of hemoglobin were observed as HbF and HbS and the rates of gene frequencies were 71.6% and 28.4% respectively

Effects on the healing process of the wound was compared between acupuncture and medicament anesthesia. Ten adult dogs were used and for medicament anesthesia thiopental sodium (Ravonal R) was injected. Incisions were made under anesthesia and thereafter clinical signs were observed and histopathological examinations were performed. No differences were found in the clinical signs between the 2 groups. Macroscopically and microscopically, the healing process was a little faster in the wounds under medicament anesthesia