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Features of the morphological structure of plants and biochemical properties of introduced and domestic varieties of Cannabis sativa L
2020
Полякова, А. С | Горшкова, Л. М | Рахметов, Д. Б
Purpose. Identifying the features of the seasonal growth rhythm, development of the plants, morphological and biological differences in monoecious introduced and domestic varieties of Cannabis sativa L. in the North-east region of Ukraine. Methods. The research was conducted from 2014 till 2019. The following research methods were employed: field (phenological observations, sampling for laboratory analysis), microscopic (study of the morphological structure of glandular and cystolithic hairs on vegetative and generative organs, determination of the size and density of hair formation), laboratory and analytical (determination of the content of cannabinoid substances by thin layer and gas-liquid chromatography), biomorphological methods (analysis of morphological characteristics of plants). Chemical analysis for the content of cannabinoid substances was carried out in the laboratory of the breeding and seed production department of the Institute of Bast Crops of the NAAS of Ukraine (Glukhiv, 2014–2019). The content of neutral substances [cannabinol (CBN), cannabidiol (CBD), tetrahydrocannabinol (THC)] and acids (CBDA, THCA) was determined by using a thin layer chromatography (TLC) and gas-liquid chromatography (GLC) methods. For the study, 11 monoecious varieties of C. sativa: ‘YuSO 1’, ‘YuSO 31’, ‘Hliana’, ‘Viktoriia’, ‘Hlesiia’, ‘Hlukhivskyi 46’, ‘Hlukhivski 51’, ‘Zolotoniski 15’ and 3 introduced varieties: ‘Fedora 17’, ‘Felina 32’, ‘Futura 77’ (France) which differed among themselves in morphological, biological, genetic, ecological-geographical and economically valuable characteristics were selected. Results. Biological and morphological characteristics of modern monoecious varieties of C. sativa were investigated depending on the ecological and geographical zones of origin, conditions of the growing season and the stage of ontogenesis. The functional interdependence between the formation of specialized excretory structures and the content of cannabinoid substances in plants of different varieties of C. sativa was revealed. The following promising varieties of C. sativa in a ration of ((THC + CBN) ≤ CBD) point the possibility to use them as the source material for creating the medicinal varieties of plants with a high content of CBD. Conclusions. Nowadays C. sativa is the main source of phyto material for industrial production of natural CBD. In recent years, high-yielding varieties of C. sativa have been created, which, together with high commercially valuable traits, did not contain psychoactive THC and had an insignificant amount of CBD. The results of the research indicated the features of the seasonal growth rhythm, development of the plants and the morphological and biological features of modern monoecious introduced and domestic varieties of C. sativa in the North-east region of Ukraine. The low correlation between the content of cannabinoid substances and their acids in varieties, which are characterized by the low content of cannabinoid compounds was established. According to the developed own classification (THC + KBN): CBD, varieties with a predominant CBD content ‘Hlukhivskyi 46’, ‘Zolotoniski 15’, ‘Hliana’ which are of significant medicinal value were identified.
显示更多 [+] 显示较少 [-]Cultivars of the Itoh peony group in the collection of the M. M. Hryshko National Botanical Garden National Academy of Sciences of Ukraine
2020
Горобець, В. Ф | Щербакова, Т. О
Purpose. To analyze the variety diversity of the Itoh peony group in the collection of the M. M. Hryshko National Botanical Garden National Botanical Garden National Academy of Sciences of Ukraine and determine its biological features in the new conditions of introduction. Methods. Introduction studies, phenological observations, morphometry, statistical processing of results. Results. The cultivars diversity of the Itoh peony group of the M. M. Hryshko National Botanical Garden National Botanical Garden National Academy of Sciences of Ukraine collection was analyzed. The ornamental properties of the cultivars were described. Features of their growth and development under conditions of introduction were studied. It was revealed that the duration of vegetation of plants of Ito group varieties is 218–225 days. It was determined that plant growth begins on March 23 – April 2, flowering on May 19–28. The budding of plants was observed in the first decade of May and lasts 16–20 days. It was revealed that in the conditions of introduction ‘Morning Lilac’ and ‘Sonoma Apricot’ bloom first (May 19–20), ‘Viking Full Moon’, ‘Yankee Doodle Dandy’, ‘Yellow Waterlily’ bloom later (May 28). The height of generative shoots of plants in flowering phase ranged from 60 to90 cm. Plants go dormant in winter, having formed generative shoots that successfully winter. Reproduction of plants occurs both due to the buds that form on the lower part of the stem, and the buds that form on the rhizome. The shoot-forming ability and flowering productivity are 6.0±2.5 – 19.5±4.0 shoots and 4.5±1.5 – 16.0±3.0 generative shoots per plant, respectively. Cultivars ‘First Arrival’, ‘Sonoma Apricot’, ‘Hillary’, ‘Bartzella’, ‘Morning Lilac’, ‘Lollipop’, ‘Old Rose Dandy’ are highly productive. Conclusions. The collection of peonies of the M. M. Hryshko National Botanical Garden National Botanical Garden National Academy of Sciences of Ukraine has been expanded by Itoh Group cultivars. The R. Anderson’s breeds of 1980–1990 are more fully represented. An analysis of the ornamental features of the varieties showed that the hybrids with a semi-double flower shape of a yellow color dominate the collection. Cultivars retain all their ornamental and economic characteristics under conditions of introduction. The Itoh group varieties of the collection can be material for developing technologies for their cultivation and propagation, can be a source of replenishment and expansion of regional botanical gardens collections, planting material for gardening and landscaping, and also perform an educational and cognitive function.
显示更多 [+] 显示较少 [-]Biomorphological characteristic of breeding samples of representatives of the genus Miscanthus, obtained in vitro
2019
Лашук, С. О
Purpose. Estimate phenological and morphological characteristics of Miscanthus giganteus J. M. Greef & Deuter ex Hodkinson & Renvoize, M. sacchariflorus (Maxim) Benth. and M. sinensis Anderss., obtained in vitro, and M. giganteus, propagated by rhizomimes (ex vitro) to attract them to the breeding process and create new forms of miscanthus for use in bioenergy. Methods. Seeds of M. sinensis, as well as M. sacchariflorus (2n), M. sacchariflorus (4n), introduced into culture and propagated in vitro according to commonly used methods (M. D. Melnychuk, A. Plazek et al.) were used in the studies. Phenological observations were carried out according to the methods of V. V. Zinchenko, M. V. Roik, D. B. Rakhmetov, and others. Statistical processing of the obtained data was carried out according to M. A. Shelamov and others. Results. M. sacchariflorus (2n) in the conditions of the Forest-Steppe of Ukraine does not enter into the flowering phase, whereas in M. sacchariflorus (4n) the flowering phase begins a month earlier than M. sinensis, which is an obstacle for transpollination of these species in the natural environment. M. giganteus, reproduced by rhizomes, in overwhelming majority of indicators (stem height and diameter, number of interstices and leaves, leaf area, length and width of cluster) dominate all species of mescanthus obtained in vitro. But the number of stems in the bush of M. sinensis is the highest (63 pcs.) and is almost 2–4 times higher than those of M. giganteus, obtained from risomes and in vitro. It has been revealed that the most promising forms for bioenergy use are M. sinensis, whose productivity is about 7 kg/m2 of green mass and M. giganteus, propagated by rhizomimes (ex vitro), where the mass of the aerial part is almost 9 kg/m2. But M. sacchariflorus (2n) and M. sacchariflorus (4n) should not be considered as promising species for use in bioenergy purposes, because their performance is very low compared to other species and is only 0.25 and 2.05 kg above ground mass from 1 m2. Conclusions. On the basis of the obtained data, the most promising forms of Miscanthus were established to attract them into the breeding process and to obtain new varieties with high biomass productivity for the needs of bioenergy.
显示更多 [+] 显示较少 [-]Features of forming a conveyor of commodity products of lettuce, Lactuca sativa L., varieties in the Western Forest-Steppe of Ukraine
2019
Лещук, Н. В | Дидів, О. Й | Хареба, О. В
Purpose. To reveal the scientific bases of forming the conveyor production of commercial products of lettuce, Lactuca sativa L., varieties in the conditions of the Western Forest-Steppe of Ukraine. Methods. Field research was conducted during 2015–2017 at the research field of the Department of Horticulture and Vegetable Growing of the Lviv National Agrarian University in accordance with the Methodology on Experimental Techniques in Vegetable and Melon Growing (2001) and Methods of Expertise of lettuce, Lactuca sativa L., varieties. Results. It is revealed that the soil and climatic conditions of the Western Forest-Steppe of Ukraine contribute to the optimal growth and development of all lettuce varieties of the corresponding types, as evidenced by the structure of conveyor receipt of fresh commodity products to the consumer (first decade of April – first decade of November). The shortest growing season was provided by lettuce varieties var. secalina ‘Zorepad, ‘Malakhit, ‘Dublianskyi’, which have the shortest period from seedlings to technical ripeness (21–42 days). Therefore, varieties of this variation occupy the largest share in the conveyor production of commercial products, which, with early spring sowing in open ground (April 14–20), comes to the consumer in the first decade of May. Winter sowing (21.11) ensured the receipt of fresh produce in the first decade of April. Commercial products by mass of edible organs, shape of the rosette of leaves (heads, stems), color, consistency and taste qualities corresponds to the technical requirements for Lactuca sativa L. Conclusions. The selection of varieties of lettuce for the conveyor production of commercial products should be carried out taking into account the variety of the corresponding type, ripeness group, and timing of sowing. The receipt of fresh lettuce to the consumer is directly dependent on the timing of seeds sowing (early spring, late spring, summer-autumn and winter).
显示更多 [+] 显示较少 [-]Features of forming a conveyor of commodity products of lettuce, Lactuca sativa L., varieties in the Western Forest-Steppe of Ukraine
2019
Н. В. Лещук | О. Й. Дидів | О. В. Хареба
Purpose. To reveal the scientific bases of forming the conveyor production of commercial products of lettuce, Lactuca sativa L., varieties in the conditions of the Western Forest-Steppe of Ukraine. Methods. Field research was conducted during 2015–2017 at the research field of the Department of Horticulture and Vegetable Growing of the Lviv National Agrarian University in accordance with the Methodology on Experimental Techniques in Vegetable and Melon Growing (2001) and Methods of Expertise of lettuce, Lactuca sativa L., varieties. Results. It is revealed that the soil and climatic conditions of the Western Forest-Steppe of Ukraine contribute to the optimal growth and development of all lettuce varieties of the corresponding types, as evidenced by the structure of conveyor receipt of fresh commodity products to the consumer (first decade of April – first decade of November). The shortest growing season was provided by lettuce varieties var. secalina ‘Zorepad, ‘Malakhit, ‘Dublianskyi’, which have the shortest period from seedlings to technical ripeness (21–42 days). Therefore, varieties of this variation occupy the largest share in the conveyor production of commercial products, which, with early spring sowing in open ground (April 14–20), comes to the consumer in the first decade of May. Winter sowing (21.11) ensured the receipt of fresh produce in the first decade of April. Commercial products by mass of edible organs, shape of the rosette of leaves (heads, stems), color, consistency and taste qualities corresponds to the technical requirements for Lactuca sativa L. Conclusions. The selection of varieties of lettuce for the conveyor production of commercial products should be carried out taking into account the variety of the corresponding type, ripeness group, and timing of sowing. The receipt of fresh lettuce to the consumer is directly dependent on the timing of seeds sowing (early spring, late spring, summer-autumn and winter).
显示更多 [+] 显示较少 [-]Agrobiological assessment of broccoli hybrids under the conditions of the Western Forest-Steppe of Ukraine
2018
Хареба, В. В | Дидів, О. Й | Дидів, І. В | Лещук, Н. В
Purpose. To conduct a comprehensive agrobiological assessment of broccoli hybrids Brassica oleracea L. convar. botrytis (L.) Alef. var. сymosa Duch. in terms of the key agronomic traits under the conditions of the Western Forest-Steppe of Ukraine. Methods. The object of the study was the following foreign broccoli hybrids: ‘Lucky F1’ (reference), ‘Batavia F1’, ‘Belstar F1’, ‘Monaco F1’, and ‘Rumba F1’. Phenological observations, biometric measurements, and records were conducted in accordance with the Methodology of Experiments in Vegetable and Melons (2001). Results. On average over the years of research, the highest yield was obtained from hybrids ‘Monaco F1’ (41.2 t/ha) and ‘Rumba F1’ (38.0 t/ha). A slightly lower yield was in ‘Belstar F1’ (32.3 t/ha) and ‘Batavia F1’ (29.6 t/ha). In general, all hybrids under investigation provided a significant yield increase (6.3–17.9 t/ha) compared to reference ‘Lucky F1’ (23.3 t/ha). The largest diameter of the main head was recorded in the hybrids ‘Rumba F1’ (19.7 cm), ‘Monaco F1’ (20.5 cm) and the smallest in ‘Lucky F1’ (14.3 cm). The weight of the main head of all hybrids was significantly higher than that of reference (295 g): ‘Batavia F1’ 451 g, ‘Belstar F1’ 477 g, ‘Rumba F1’ 514 g, and ‘Monaco F1’ 542 g. The weight of the side heads of the broccoli ranged from 328 g (‘Lucky F1’) to 807 g (‘Monaco F1’). The highest biochemical indicators of commodity product were provided by hybrids ‘Rumba F1’ and ‘Monaco F1’: the total dry matter content was 11.3 and 11.6%, soluble solids 6.4 and 6.7%, sugars 3.5 and 3.8%, and vitamin C 89.3 and 98.7 mg/100 g, respectively. Conclusions. Under the conditions of the Western Forest-Steppe of Ukraine, it is recommended to grow new foreign broccoli hybrids ‘Rumba F1’ and ‘Monaco F1’, which ensure high yield and quality of commodity product. According to the results of the agrobiological assessment, a typical model of broccoli variety is proposed, which may serve as a project for the development of the evaluation form for ability indicators of Brassica oleracea L. convar. botrytis (L.) Alef. var. сymosa Duch. for distribution inUkraine.
显示更多 [+] 显示较少 [-]Quality and marketable characteristics of highbush blueberry (Vaccinium corym- bosum L.) under the Kyiv region conditions
2013
Сіленко, В. О | Марченко, С. В
In the article the authors present the results of the study biometric and qualitative characteristics of berries highbush blueberry varieties (Vaccinium corymbosum L.) in a specific soil and climatic conditions Forest-steppe of Ukraine (Kiev). Investigations were carried out on 11 varieties of American highbush blueberry. Phenological observations, biometric surveys, tasting score and biochemical analysis of the berries have been conducted during the growing season. By results of researches the group of early-ripening varieties includes Bluestar, Earliblue, Patriot, the middle-ripening – Jonne, Atlantis, Bluegold, Bluecrop, Chyk and late-ripening varieties – Amanda, Darrow, Toro. Duration of collecting period of ripe berries was lower (16 days) in varieties Bluestar, Earliblue, Jonne and Darrow. Berries were large sizes in the varieties of Darrow, Amanda, Jonne, in other varieties berries were medium size. By tasting score the berry of varieties Toro, Earliblue, Jonne and Darrow were the best. For biochemical parameters the better varieties were Amanda, Jonne and Earliblue.
显示更多 [+] 显示较少 [-]Growth and development of sugar sorghum (Sorghum saccharatum L. Pers.) plants at different terms of sowing and seeding depth in the Forest-Steppe of Ukraine
2013
Герасименко, Л. А
The article features the results of research on phenological observations, biometric parameters of growth and development of the plants of sugar sorghum Silosnoe 42 variety during the vegetation at different terms of sowing and seeding depth in the Forest-Steppe of Ukraine. In particular, we show data on ground germination capacity of the seeds, the duration of the interphase periods, tillering, plant height and the diameter of the stem. It was established that the planting of sugar sorghum in mid-May (the third sowing date) to the seeding depth of 4...6 cm enabled better plant development, as well as in these conditions the maximum values of plant growth and development were registered compared to the other test editions. Therefore, were would suggest the third term of sowing and seed depth 4...6 cm for growing the crop in this area.
显示更多 [+] 显示较少 [-]Methodological aspects of applying histogram and variation curve of morphological traits of Maize (Zea mays L.) hybrids
2013
Лещук, Н. В | Таганцова, М. М | Стадніченко, О. А
The article provides methodological ground (method of morphological description) for identification of qualitative, quantitative and pseudo-qualitative characteristics of maize hybrids (Zea mays L) disclosing methodological facets of practical appliance for histogram and variation curve of morphological characteristics of the phenotype by uniformity test and unveiling the link between quantitative characteristics of the ear and economically valuable indices of plant productivity. Target of the research has covered physiological and biochemical processes of plant productivity development into relevant phenological growth and development stages followed by relevant observations and records of vegetative and generative organs of maize. The field examination results obtained are processed with mathematical and statistical methods with the view of searching for margins (min and max) of confidence intervals and in order to verify reliability of the research results with the view of follow-up construction of histogram and variation curve.
显示更多 [+] 显示较少 [-]Customer-related quality of perspective potato hybrids (Solanum tuberosum L.)
2020
Кожушко, Н. С | Сахошко, М. М | Бердін, С. І | Баштовий, М. Г | Смілик, Д. В
Purpose. Based on the study of consumer qualities of perspective potato hybrids (Solanum tuberosum L.) the ones with a high degree of differentiation depending on the biochemical composition of tubers and culinary-consumer type were identified. Methods. Field (phenology); laboratory (biochemical, technological); mathematical and statistical (linear correlation, regression). Results. Consumer qualities and patterns of their formation under the influence of the biochemical composition of tubers were studied. Excellent taste was determined in mid-early (‘203.703-3’, ‘205.781-9’), mid-season (‘205.786-72’, ‘205. 798-15’) hybrids. High cooking quality of the tubers was detected in early-season hybrids (‘99.523-15’, ‘205.791-3’, ‘205.785-25’, ‘205.792-205’). Soft crispy texture was detected in mid-early (‘205.776-25’, ‘99.546-3’) and mid-season (‘205.782-24’, ‘205.786-72’, ‘205.798-43’) hybrids. The mid-early hybrid '205.780-8' contained high levels of starch. In terms of resistance to browning, three early-season (‘99.517-39’, ‘203.703-2’, ‘205.785-25’) and two mid-season hybrids (‘205.781-9’, ‘205.788-3’) were selected. It is proved, that the taste development depended on the starch content (r=0,657), proportion of starch/protein (r=0,470) and sugars accumulation (r= -450). The positive dependence of the cooking quality of the tubers on their starchiness (r=0,945), proportion of starch and protein (r=0,900) was detected. The influence of proportion of starch/protein over the texture of the tubers (r=0,877) and starch content (r=0,868) was established. Mealiness depended on sugars (r= -0,494) and starch content (r=0,474), resistance to browning – on tyrosine content in protein (r=-0,873) and free tyrosine content (r= - 0,824). In terms of culinary and consumer type of food (table) potatoes, eight hybrids were rated as type B, five and five hybrids belonged to types A and C respectively, and three hybrids belonged to type D. Conclusions. The selected, as for the high level of culinary quality indicators, hybrids can be recommended as valuable output material for practical plant breeding. Determination of biological interrelation of potato culinary quality indicators and biochemical composition of their tubers allowed to develop mathematical simulations to predict taste and texture, and it practically relieved from boiling and subjective evaluation by organoleptic method of tasting results. Systematization of culinary and consumer type food (table) potatoes can be a subject matter in the course of state variety testing.
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