Purpose. Analysis of the process of hexaploid triticale breeding for winter hardiness by intraspecific hybridization method using systemic ecological tests under contrasting conditions. Methods. Dialectical investigation, field experiments, laboratory testing and statistical evaluation. Results. The results of breeding winter and alternate triticale varieties possessing a complex of valuable traits by the method of intraspecific hybridization of forms of different types of development using systemic ecological tests under contrasting conditions (Forest-Steppe – extremely arid Steppe) and at low temperatures were presented. During the years of research (1980–2005), 18 varieties were developed and transferred to the state testing, 17 of them were registered. Conclusions. Thus, the effective selection of highly productive genotypes with increased and high winter hardiness is possible from populations obtained by crossing hexaploid triticale of different types of development (winter triticale with spring and alternate ones) and contrasting level of frost and winter hardiness. During the period of 1980–2015, medium-tall and dwarf varieties of winter (‘Amfidyploid 256’, ‘Garne’, ‘Kharroza’, ‘Rarytet’, ‘Timofey’, etc.) and alternate (‘Nikanor’, ‘Yaroslava’, ‘Plastun Volynskyi’) triticales were developed. They are superior to the standard varieties of soft winter wheat for the critical temperature of freezing by -0.5...- 2.0 °C, characterized by increased (up to 9–12 t/ha) grain yield of various quality depending on the purpose of use.

The results obtained during observations of the growing season of pea collection varieties in different years in terms of interphase periods are presented. The dependence of the growing season length for varieties of various maturity groups on the duration of certain phases of plant growth (seedling, flowering, ripening), ambient environment (temperature, humidity, daylight hours), processing (seed preparation, line production maintenance) and genetic factors (sequential node of the first inflorescence formation) are shown. According to the study results the varieties-sources of «growing season length» characteristic were specified that have been used as components for breeding in the selection process when creating new domestic varieties.

Results of study focusing on impact of environmental factor – time of spring vegetation renewal (TSVR) of soft winter wheat on growth and development of plants, crop productivity and modern varieties response are presented. It is found that in the central part of the Right-Bank of Forest-Steppe of Ukraine this factor is important and it should be considered in planning of spring and summer care techniques, fertilizer system, especially at spring fertilizing, use of pesticides and growth regulators, in taking a decision on reseeding or underseeding of space plants. At the same time, it was determined that the environmental effect of TSVR was not occurred every year, thus it is not always possible to forecast the type of plant development. But in such years it is possible to influence the processes of plants growth, development and survival in spring and summer periods and the formation of their productivity by introducing such intensive technologies as differential crop tending, mineral nutrition optimization, the use of plant growth regulators, trace nutrients, weed, pest and disease control agents.

In the Mironivka Institute for wheat named by V.M. Remeslo UAAN new winter-hardy winter wheat variety material was obtained. The annual breeding was used for the selection df winter wheat forms from spring wheat varieties by the prevernalization, spring sowing and M2 at the beginning of optimal terms in autumn (the influence of low temperature on spring wheat seeds). On the basis of such material, winter-hardy wheat varieties were created, such as Myronivs’ka rannyostygla, Remeslivna, Vo- loshkova and such varieties as Bagira, Vdyachna and Svyankova were submitted to the State Service for Plant Variety Protection.

In the article there are surveyed approaches to the use of biological - ecological potential of white head cabbage plants, given parameters of varieties adaptability and economically-valuable signs variability, depending on a temperature factor, water supply. The yield prognosis is worked out.

Variations in bread volume output in accordance with external temperature factor which balancing increases obtained information reliability are considered.

Purpose. Analysis of the current state and experience on the loop-mediated amplification (LAMP) use to detect genetically modified plants. Methods. Literature search and analysis. Results. General information on the current state and use of the genetically modified plants is provided. Despite the wide distribution of genetically modified plants, the attitude towards them in society continues to remain somewhat wary. About 50 countries have introduced mandatory labeling of GM feed and products, provided that their content exceeds a certain threshold. In order to meet labeling requirements, effective and sensitive methods for detecting known genetic modifications in a variety of plant materials, food products and animal feed must be developed and standardized. The most common approaches to the detection of genetically modified organisms (GMOs) are the determination of specific proteins synthesized in transgenic plants and the detection of new introduced genes. Methods for the determination of GMOs based on the analysis of nucleic acids are more common, since such methods have greater sensitivity and specificity than the analysis of protein composition. Polymerase chain reaction (PCR) method is the main method of nucleic acid analysis, which is now wide used for the detection of GMOs. Loop-mediated amplification (LAMP), which can occur at a constant temperature and therefore does not require the use of expensive equipment may be an alternative to the PCR. Scientific articles about the use of the loop-mediated amplification (LAMP) for the detection of genetically modified plants were analyzed. Advantages and disadvantages of the polymerase chain reaction and loop-mediated amplification are compared. Conclusions. The main criteria for applying a method of GMO detection analysis are as follow: its sensitivity, time of reaction, availability and ease to use, cost of reagents and equipment, and the possibility for simultaneous detection of many samples.

Purpose. To establish correlation dependence of influence of such factors as seeding rate, sowing time and seeding methods on yield indicators, as well as individual response of winter wheat varieties to them in case of the ecological system of seed production, that would allow to form elements of intensive varietal technology and obtain the genetically determined productivity potential of the variety in the future. Methods. Weight measuring was used to determine seed yield; the reliability of the experiment results was identified by statistical evaluation, analysis of variance and regression procedure. Results. During the period of investigation, the weather conditions differed significantly both for the temperature regime and the amount of precipitation, but the stability of grain and seed yield over years was the main requirement to varieties, that allowed to study the intergenotypic correlation relationships of crop yield indices and the dynamics of their changes under the effect of ecological factors. In the course of investigation, winter wheat yield indices were fixed annually from 684 plots, which were grouped and analyzed for factors of influence in order to ensure the complete certainty. Conclusions. Variants of investigation were defined for the certified seed yield and environmental factors (sowing time and seeding method, seeding rate), that had strong positive correlation relationships. Seeding rate and seeding method had the greatest impact on 1000 kernel weight. For tille­ring coefficient, varieties depending on the variant of the investigation had a negative close correlation relationship, which was greatly affected by seeding rate. For correlation relationships, winter wheat varieties ‘Bohdana’ and ‘Slavna’ appeared to be the most stable, ‘Chorniava’ and ‘Astarta’ were the most plastic.

Purpose. To create a multiplex system for identification glyphosate-tolerant sugar beet by using PCR. Methods. Molecular genetic analysis. Results. The article presents the results of studies to determine the parameters of the polymerase chain reaction (PCR) in order to develop a multiplex system for identification of the structural elements of the design of transgenic gene cp 4 epsps, which provides tole­rance to glyphosate. For amplicon target DNA sequences, the following values of temperature conditions of PCR were determined: step 1 (initial denaturation) 95 °C – 3 min; step 2 (specific reaction products accumulation): denaturation 95 °C – 45 s; hybridization of primers 55 °C – 50 s; elongation 72 °C – 1 min; number of cycles – 40; step 3 (final elongation) 72 °C – 6 min. A series of PCR were carried out for the purpose of selecting the optimal amount of DNA matrix for efficient estimate of transgenic sugar beet plants for the presence of specific sequences. Conclusions. To identify transgenic glyphosate-tolerant sugar beet, it is advisable to determine 35S promoter and gene cp 4 epsps in individual genotypes. It was found that during the selection of temperature parameters of multiplex reaction a 5 °C rise in primer hybridization temperature did not affect the identification of gene als that allowed to include specific primers for determination of this sequence as an internal control. Based on the results of test multiplex reactions, concentrations of dNTPs and Mg2+ ions were determined that allowed to exclude the possibility of non-specific fragments and false-negative results. The optimum amount of matrix DNA (100–150 ng) for an efficient estimate of transgenic sugar beet plants for the presence of specific sequences was determined. Obtained results allowed to develop a multiplex test system for identification of transgenic glyphosate-to­lerant sugar beet which can be used for simultaneous determination of the 35S promoter, cp 4 epsps gene and als gene as an internal reaction control.

The article presents the results of the laboratory investigation of the influence of high temperatures on water regime of red currant in Institute of Fruit Selection. It shows the dependence of water loss by leaves on the phenophase of plant development. High water loss by leaves occurs during the phase of ripening berries (July). Low water loss is noted during the active growth of shoots (June). Red currant genotypes: «Valentinovka», «Bayna» have a high percent of water loss in June and July. Genotypes «Shedraya», «Gollandskay krasnay», «Orlovchanka», «Dar Orla», «Selyanochka», «Osipovskay», «Belka» and «Jonker van Tets» have a low percent of loss water. The loss of water at the temperature shock is the important protective mechanism from over heat and death of plants. The results of the investigation of a degree of water restoration by red currant leaves in the periods of berry ripening and active growth of shoots are given. The most part of genotypes of red currant leaves have a high percent (>50%) of water restoration. Genotypes «Selyanochka» ,«Orlovchanka», «Dana», «Shedraya», «Belka» have a more 100% of water restoration. On the ground of the obtained results the following genotypes: «Belka», «Selyanochka», «Gollandskay krasnay», «Shedraya», «Orlovchanka», «Dar Orla», «Osipovskay» and «Jonker van Tets» are mostly heat resistant.